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The map label for this gene is ltaA [H]

Identifier: 108758654

GI number: 108758654

Start: 1689251

End: 1690276

Strand: Direct

Name: ltaA [H]

Synonym: MXAN_1432

Alternate gene names: 108758654

Gene position: 1689251-1690276 (Clockwise)

Preceding gene: 108761299

Following gene: 108757855

Centisome position: 18.48

GC content: 71.44

Gene sequence:

>1026_bases
ATGAAGCGCATCGACTTCCGTTCCGACACCGTCACCCAGCCCACGGCCGCCATGCGGCGCGCCATCGCCGAAGCCGAAGT
AGGGGATGACGTTTATGGCGAAGACCCCACCGTGCTCCGTCTGGAGGCACGGGTGGCCGAGCGGCTCGGCCTGGAGGCCG
CCGTCTTCGTGCCCTCGGGGACGCAGGCCAACCAGTTGGCCATCGGCGCGCATTGCCGGGCGGGCGACGAGGTGCTCACC
GAAGCAGGCAGCCACATCCTCCACTACGAAGGGGGCGCGGTGCCCGCCTTGTGGGGCGTGCAGCCGCAGCCACTGCCAGG
GCAGCGCGGTCTGCTGTCGCCGGAGGATGTGAGCGCGGCCGTGCGCGAGGACAACATCCACAACCCGCGCACGCGCCTGC
TGTCGCTGGAGAACACGCACAACCGGGGTGGTGGTTCGGTATGGCCGGTGGAGCGCTTCCGGGCCGTCGTGGAAGCGGCG
CGCAAGGCGGGGTTGGCCGTCCACCTGGATGGAGCCAGGCTCTTCAACGCCGGTGTCGCCCTGGGCGTGCCGGTGTCCGC
CTGGGCCGCGCTGACGGACACGACGTCCGTGTGTTTCTCGAAGGGCCTGGGCGCTCCGGTGGGCTCGGCCCTGGCGGGGC
GCGCGGACGTCATTCGCGAGGCGCGCCGGCTGCGCAAGCGGCTGGGCGGTGGCATGCGGCAGGCAGGCATCCTGGCGGCG
GCCGCGCTGCATGCACTGGAGCACCATGTAGAGCGGCTGGCGGATGACCATGCGAACGCCCGCAGGCTGGCCGCGGGGCT
GGCGGAGCTGCCCGGCGTGGAGGTGGACGCTTCGCGGGTGGAGACCAACATGGTCTTCGCGGAGTTCTCCCGGTCCGCTC
TGGAGATGGTGGCACGACTGGAGCAGCACGGGGTCCTGACGAACCCCGCGGGTGCTCCTCGGACGCTGCGCCTCGTCACC
CACCTGGATGTGTCCGCTGCCGACATCGACGAAGCCCTGCAGCGCATCCGCCGCGCCGTGGGCTGA

Upstream 100 bases:

>100_bases
CGCTTCTCCGCGACCGTCTCACGTCCAGGCACGGCGCCGTTGTATGTCACACCGGCTGCGAGCCCAAGCACCGGTTGGAC
GCACCCATTCGGGGAGATTC

Downstream 100 bases:

>100_bases
GCACAGCCTTGGAGTGAAACGCGCGTCCGTGGACATCCCTGGGCGCGCACACGGGGCGGGGAAGGAGCGCTTCGGGCGGG
CCATGGTAGGCTCGCACCGC

Product: L-allo-threonine aldolase

Products: glycine; acetaldehyde

Alternate protein names: L-allo-TA; L-allo-threonine acetaldehyde-lyase [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MKRIDFRSDTVTQPTAAMRRAIAEAEVGDDVYGEDPTVLRLEARVAERLGLEAAVFVPSGTQANQLAIGAHCRAGDEVLT
EAGSHILHYEGGAVPALWGVQPQPLPGQRGLLSPEDVSAAVREDNIHNPRTRLLSLENTHNRGGGSVWPVERFRAVVEAA
RKAGLAVHLDGARLFNAGVALGVPVSAWAALTDTTSVCFSKGLGAPVGSALAGRADVIREARRLRKRLGGGMRQAGILAA
AALHALEHHVERLADDHANARRLAAGLAELPGVEVDASRVETNMVFAEFSRSALEMVARLEQHGVLTNPAGAPRTLRLVT
HLDVSAADIDEALQRIRRAVG

Sequences:

>Translated_341_residues
MKRIDFRSDTVTQPTAAMRRAIAEAEVGDDVYGEDPTVLRLEARVAERLGLEAAVFVPSGTQANQLAIGAHCRAGDEVLT
EAGSHILHYEGGAVPALWGVQPQPLPGQRGLLSPEDVSAAVREDNIHNPRTRLLSLENTHNRGGGSVWPVERFRAVVEAA
RKAGLAVHLDGARLFNAGVALGVPVSAWAALTDTTSVCFSKGLGAPVGSALAGRADVIREARRLRKRLGGGMRQAGILAA
AALHALEHHVERLADDHANARRLAAGLAELPGVEVDASRVETNMVFAEFSRSALEMVARLEQHGVLTNPAGAPRTLRLVT
HLDVSAADIDEALQRIRRAVG
>Mature_341_residues
MKRIDFRSDTVTQPTAAMRRAIAEAEVGDDVYGEDPTVLRLEARVAERLGLEAAVFVPSGTQANQLAIGAHCRAGDEVLT
EAGSHILHYEGGAVPALWGVQPQPLPGQRGLLSPEDVSAAVREDNIHNPRTRLLSLENTHNRGGGSVWPVERFRAVVEAA
RKAGLAVHLDGARLFNAGVALGVPVSAWAALTDTTSVCFSKGLGAPVGSALAGRADVIREARRLRKRLGGGMRQAGILAA
AALHALEHHVERLADDHANARRLAAGLAELPGVEVDASRVETNMVFAEFSRSALEMVARLEQHGVLTNPAGAPRTLRLVT
HLDVSAADIDEALQRIRRAVG

Specific function: Stereospecifically catalyzes the interconversion of L- allo-threonine and glycine [H]

COG id: COG2008

COG function: function code E; Threonine aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1787095, Length=324, Percent_Identity=46.2962962962963, Blast_Score=261, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI193206520, Length=341, Percent_Identity=43.6950146627566, Blast_Score=233, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI193206522, Length=341, Percent_Identity=43.6950146627566, Blast_Score=233, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI193206524, Length=341, Percent_Identity=43.6950146627566, Blast_Score=233, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6320789, Length=287, Percent_Identity=37.9790940766551, Blast_Score=177, Evalue=2e-45,
Organism=Drosophila melanogaster, GI21355439, Length=368, Percent_Identity=42.3913043478261, Blast_Score=246, Evalue=1e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: 4.1.2.5

Molecular weight: Translated: 36219; Mature: 36219

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIDFRSDTVTQPTAAMRRAIAEAEVGDDVYGEDPTVLRLEARVAERLGLEAAVFVPSG
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHCCEEEEEECCC
TQANQLAIGAHCRAGDEVLTEAGSHILHYEGGAVPALWGVQPQPLPGQRGLLSPEDVSAA
CCCCCEEECCCCCCCHHHHHHCCCEEEEECCCCCCEECCCCCCCCCCCCCCCCHHHHHHH
VREDNIHNPRTRLLSLENTHNRGGGSVWPVERFRAVVEAARKAGLAVHLDGARLFNAGVA
HHHCCCCCCHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCHHHHHCCEE
LGVPVSAWAALTDTTSVCFSKGLGAPVGSALAGRADVIREARRLRKRLGGGMRQAGILAA
ECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
AALHALEHHVERLADDHANARRLAAGLAELPGVEVDASRVETNMVFAEFSRSALEMVARL
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EQHGVLTNPAGAPRTLRLVTHLDVSAADIDEALQRIRRAVG
HHCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRIDFRSDTVTQPTAAMRRAIAEAEVGDDVYGEDPTVLRLEARVAERLGLEAAVFVPSG
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHCCEEEEEECCC
TQANQLAIGAHCRAGDEVLTEAGSHILHYEGGAVPALWGVQPQPLPGQRGLLSPEDVSAA
CCCCCEEECCCCCCCHHHHHHCCCEEEEECCCCCCEECCCCCCCCCCCCCCCCHHHHHHH
VREDNIHNPRTRLLSLENTHNRGGGSVWPVERFRAVVEAARKAGLAVHLDGARLFNAGVA
HHHCCCCCCHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCHHHHHCCEE
LGVPVSAWAALTDTTSVCFSKGLGAPVGSALAGRADVIREARRLRKRLGGGMRQAGILAA
ECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
AALHALEHHVERLADDHANARRLAAGLAELPGVEVDASRVETNMVFAEFSRSALEMVARL
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EQHGVLTNPAGAPRTLRLVTHLDVSAADIDEALQRIRRAVG
HHCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 1.7

Km value (mM): 2.85 {L-Thr}} 0.12 {DL-threo-phenylserine}} 0.24 {DL-erythro-phenylserine}} 0.22 {L-allo-Thr}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = glycine + acetaldehyde

General reaction: Elimination of an aldehyde, C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9171400; 9228760 [H]