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The map label for this gene is def [H]

Identifier: 108761299

GI number: 108761299

Start: 1687979

End: 1688512

Strand: Direct

Name: def [H]

Synonym: MXAN_1430

Alternate gene names: 108761299

Gene position: 1687979-1688512 (Clockwise)

Preceding gene: 108762878

Following gene: 108758654

Centisome position: 18.47

GC content: 64.61

Gene sequence:

>534_bases
ATGGTCCGTGAGATCCTCATCTGGCCCGACCCTGTCCTGAAACAGAAGGCCAAGCCGGTGGCGAAGGTGGATGACTCCAC
CCGCACGCTGGTGAAGGACATGTTCGAAACGATGTACTCCGCTGAAGGCGTGGGCCTGGCCGCCCCGCAAATCGGCGTGC
TCCAGCGCGTCATCGTGCTGGACACCACGCACAGCCAGCCGGAGCTGAAGCCCGTTGCGATGATCAATCCGGAGATCATC
GCCATGGAAGGCGACACGACCTACAACGAGGGCTGCCTGTCCATCCCTGGTGAGGCCGCGGATGTCGACCGGGCCGCCGT
GGTCACCGTGAAGTTCCTGGACCCGGACGGTCAGGAGCAGACGCTGCGCTGTGACGGGCTGCTGTCCATCGCGGTGCAGC
ACGAGACGGACCACCTCAACGGCACCGTCTTCGTGGACCACATTTCGTCCCTGAAGCGCGAGTTCATCCGCAAGCGGATG
AAGCGCCTCAAGACTTCGCGCGAGCGCGAGGCGAGCGCGCCGGCGTCTCCGTAG

Upstream 100 bases:

>100_bases
TGCTCGACTGACGCCCGTCGGACGGACGGCCTCCCGGGAGTGCAGGGCGGCCCACCCGGGGCGGTTGCCCGTCCGCTCCG
GGGAGATTAAGACGGCGTCC

Downstream 100 bases:

>100_bases
CGCCGGCGTCCTTCTGGACCTTCACGCCCTTCTTCGGGCACAGGTCCGCGACGACGCAGCGCTCACACGCGGGCGAGCGG
GCAAAGCAGGTCCGCCGCCC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MVREILIWPDPVLKQKAKPVAKVDDSTRTLVKDMFETMYSAEGVGLAAPQIGVLQRVIVLDTTHSQPELKPVAMINPEII
AMEGDTTYNEGCLSIPGEAADVDRAAVVTVKFLDPDGQEQTLRCDGLLSIAVQHETDHLNGTVFVDHISSLKREFIRKRM
KRLKTSREREASAPASP

Sequences:

>Translated_177_residues
MVREILIWPDPVLKQKAKPVAKVDDSTRTLVKDMFETMYSAEGVGLAAPQIGVLQRVIVLDTTHSQPELKPVAMINPEII
AMEGDTTYNEGCLSIPGEAADVDRAAVVTVKFLDPDGQEQTLRCDGLLSIAVQHETDHLNGTVFVDHISSLKREFIRKRM
KRLKTSREREASAPASP
>Mature_177_residues
MVREILIWPDPVLKQKAKPVAKVDDSTRTLVKDMFETMYSAEGVGLAAPQIGVLQRVIVLDTTHSQPELKPVAMINPEII
AMEGDTTYNEGCLSIPGEAADVDRAAVVTVKFLDPDGQEQTLRCDGLLSIAVQHETDHLNGTVFVDHISSLKREFIRKRM
KRLKTSREREASAPASP

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=159, Percent_Identity=30.8176100628931, Blast_Score=78, Evalue=4e-15,
Organism=Escherichia coli, GI1789682, Length=168, Percent_Identity=44.6428571428571, Blast_Score=149, Evalue=9e-38,
Organism=Drosophila melanogaster, GI24645728, Length=157, Percent_Identity=30.5732484076433, Blast_Score=80, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24645726, Length=157, Percent_Identity=31.2101910828025, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19563; Mature: 19563

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVREILIWPDPVLKQKAKPVAKVDDSTRTLVKDMFETMYSAEGVGLAAPQIGVLQRVIVL
CCCEEEECCCHHHHHHCCCCHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHEEEEE
DTTHSQPELKPVAMINPEIIAMEGDTTYNEGCLSIPGEAADVDRAAVVTVKFLDPDGQEQ
ECCCCCCCCCEEEEECCEEEEEECCCCCCCCCEECCCCCCCCCCEEEEEEEEECCCCCCC
TLRCDGLLSIAVQHETDHLNGTVFVDHISSLKREFIRKRMKRLKTSREREASAPASP
EEEECCEEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MVREILIWPDPVLKQKAKPVAKVDDSTRTLVKDMFETMYSAEGVGLAAPQIGVLQRVIVL
CCCEEEECCCHHHHHHCCCCHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHEEEEE
DTTHSQPELKPVAMINPEIIAMEGDTTYNEGCLSIPGEAADVDRAAVVTVKFLDPDGQEQ
ECCCCCCCCCEEEEECCEEEEEECCCCCCCCCEECCCCCCCCCCEEEEEEEEECCCCCCC
TLRCDGLLSIAVQHETDHLNGTVFVDHISSLKREFIRKRMKRLKTSREREASAPASP
EEEECCEEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA