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The map label for this gene is rlmL [H]

Identifier: 108758528

GI number: 108758528

Start: 2363380

End: 2364408

Strand: Reverse

Name: rlmL [H]

Synonym: MXAN_2032

Alternate gene names: 108758528

Gene position: 2364408-2363380 (Counterclockwise)

Preceding gene: 108763044

Following gene: 108760026

Centisome position: 25.87

GC content: 69.0

Gene sequence:

>1029_bases
ATGAGGGGGGCCGCGGTGGTCTGGACGGAAGGACGCTTGGGCATGGCTGACACACAAGGTACGCATGGCTCTCACGACGC
AACCGGCCCCGAGGGCATGTTCGCCAACCGCCTGCGCAAGGGGGACAAGCGCTTCCGGAAATGGGCCCGGACGCAGGGGC
TGACGGCCTTCCGCGTCTACGACAGGGACATCCCCGAGTACCCGTACGCCATCGACGTCTACGGCGACCGCGTCCACGTC
GTGGAATACCCCCGGCGGCGTGCCATCGCCTCCGGTGCCGCGGACACGCAGCGGGAGGAAGTCCTGGCCGCAGTGACGCA
GGTGCTGGCCGTTCCCTCCGAGCGCGTCTTCGTGAAGACGCACACGCCCCAGCCCTGGGGCCGCTCTCAGTACGGCCGGG
TGGGCCAGGACAGCGGGCGCATCGTGGTGGAGGAGCAGGGCCTGAAGTTCTGGGTGAACCTGGGCGACTACCTCGACACC
GGCCTCTTCATGGACCACCGCAACACCCGCGCGCGCGTGCGGGAGGAGGCCCGGGGCAAGCGCTTCCTCAACCTCTTCGC
GTACACCGGGGCCTTCACCGTCTACGCCGCCGCGGGAGGCGCCGCGAGCACGATGACGGTGGACCTGTCCAACACCTACC
TGGACTGGGCCGAGGACAACCTGGACCTCAACGGCCTGGCCGACGCGCGGCACACGCTGGTGCGCGCGGATGCCAAGGCC
TGGGTGGAGGCCCAGGCCGACGCGCCGGAGCGGTACGACCTGGTGGTGTGCGACCCGCCGTCCTTCTCCACGTCGAAGAA
GATGTCGGGCAGCTTCAACGTGCAGCGCGACCACCCGCGCCTGCTGGCCGCCATCCGGGCGCTGCTCGCACCCGGTGGCG
TCCTCTACTTCTCCACCAACTTCCTGGGGTTCCAGTTGGAACCGAAGGCCACGCGGGGAATGGAGGTGGAGGAAATCACC
CCTCGCTCCATCCCCGAGGACTTCCAGCGCAAGGAGATCCACCGCTGCTGGCGCATGGTGGCTCCCTGA

Upstream 100 bases:

>100_bases
GGGCTCGCCAAAGACAGTCTGGGTGCCCGTGCGCCAGAGCGGACAGGCCTGACACCCCGCAGCCGCCTTTCGGAGCTTGT
CGTACGTCGGAGACTCAGGG

Downstream 100 bases:

>100_bases
CGCTGGCGTCCGTCCCGACTACAGCCAGCAGACGTTGTCGATGCAGCGCTCGCCGGCGGGGCACTCGCAGGTGGCGTGAC
AGCTGTTGCCGCTGCCCGTG

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MRGAAVVWTEGRLGMADTQGTHGSHDATGPEGMFANRLRKGDKRFRKWARTQGLTAFRVYDRDIPEYPYAIDVYGDRVHV
VEYPRRRAIASGAADTQREEVLAAVTQVLAVPSERVFVKTHTPQPWGRSQYGRVGQDSGRIVVEEQGLKFWVNLGDYLDT
GLFMDHRNTRARVREEARGKRFLNLFAYTGAFTVYAAAGGAASTMTVDLSNTYLDWAEDNLDLNGLADARHTLVRADAKA
WVEAQADAPERYDLVVCDPPSFSTSKKMSGSFNVQRDHPRLLAAIRALLAPGGVLYFSTNFLGFQLEPKATRGMEVEEIT
PRSIPEDFQRKEIHRCWRMVAP

Sequences:

>Translated_342_residues
MRGAAVVWTEGRLGMADTQGTHGSHDATGPEGMFANRLRKGDKRFRKWARTQGLTAFRVYDRDIPEYPYAIDVYGDRVHV
VEYPRRRAIASGAADTQREEVLAAVTQVLAVPSERVFVKTHTPQPWGRSQYGRVGQDSGRIVVEEQGLKFWVNLGDYLDT
GLFMDHRNTRARVREEARGKRFLNLFAYTGAFTVYAAAGGAASTMTVDLSNTYLDWAEDNLDLNGLADARHTLVRADAKA
WVEAQADAPERYDLVVCDPPSFSTSKKMSGSFNVQRDHPRLLAAIRALLAPGGVLYFSTNFLGFQLEPKATRGMEVEEIT
PRSIPEDFQRKEIHRCWRMVAP
>Mature_342_residues
MRGAAVVWTEGRLGMADTQGTHGSHDATGPEGMFANRLRKGDKRFRKWARTQGLTAFRVYDRDIPEYPYAIDVYGDRVHV
VEYPRRRAIASGAADTQREEVLAAVTQVLAVPSERVFVKTHTPQPWGRSQYGRVGQDSGRIVVEEQGLKFWVNLGDYLDT
GLFMDHRNTRARVREEARGKRFLNLFAYTGAFTVYAAAGGAASTMTVDLSNTYLDWAEDNLDLNGLADARHTLVRADAKA
WVEAQADAPERYDLVVCDPPSFSTSKKMSGSFNVQRDHPRLLAAIRALLAPGGVLYFSTNFLGFQLEPKATRGMEVEEIT
PRSIPEDFQRKEIHRCWRMVAP

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=311, Percent_Identity=45.016077170418, Blast_Score=275, Evalue=2e-75,
Organism=Escherichia coli, GI145693111, Length=276, Percent_Identity=29.3478260869565, Blast_Score=101, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 38388; Mature: 38388

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGAAVVWTEGRLGMADTQGTHGSHDATGPEGMFANRLRKGDKRFRKWARTQGLTAFRVY
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
DRDIPEYPYAIDVYGDRVHVVEYPRRRAIASGAADTQREEVLAAVTQVLAVPSERVFVKT
CCCCCCCCEEEEEECCEEEEEECCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEE
HTPQPWGRSQYGRVGQDSGRIVVEEQGLKFWVNLGDYLDTGLFMDHRNTRARVREEARGK
CCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCHHHCCCEEEECCCHHHHHHHHHHHH
RFLNLFAYTGAFTVYAAAGGAASTMTVDLSNTYLDWAEDNLDLNGLADARHTLVRADAKA
HHHHHHHHHCCEEEEEECCCCCEEEEEECCCCEECCCCCCCCCCCCCHHHHHHHHHCHHH
WVEAQADAPERYDLVVCDPPSFSTSKKMSGSFNVQRDHPRLLAAIRALLAPGGVLYFSTN
HEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECC
FLGFQLEPKATRGMEVEEITPRSIPEDFQRKEIHRCWRMVAP
EEEEEECCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRGAAVVWTEGRLGMADTQGTHGSHDATGPEGMFANRLRKGDKRFRKWARTQGLTAFRVY
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
DRDIPEYPYAIDVYGDRVHVVEYPRRRAIASGAADTQREEVLAAVTQVLAVPSERVFVKT
CCCCCCCCEEEEEECCEEEEEECCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEE
HTPQPWGRSQYGRVGQDSGRIVVEEQGLKFWVNLGDYLDTGLFMDHRNTRARVREEARGK
CCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCHHHCCCEEEECCCHHHHHHHHHHHH
RFLNLFAYTGAFTVYAAAGGAASTMTVDLSNTYLDWAEDNLDLNGLADARHTLVRADAKA
HHHHHHHHHCCEEEEEECCCCCEEEEEECCCCEECCCCCCCCCCCCCHHHHHHHHHCHHH
WVEAQADAPERYDLVVCDPPSFSTSKKMSGSFNVQRDHPRLLAAIRALLAPGGVLYFSTN
HEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECC
FLGFQLEPKATRGMEVEEITPRSIPEDFQRKEIHRCWRMVAP
EEEEEECCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA