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The map label for this gene is yafJ [H]

Identifier: 108758393

GI number: 108758393

Start: 5351641

End: 5352474

Strand: Direct

Name: yafJ [H]

Synonym: MXAN_4346

Alternate gene names: 108758393

Gene position: 5351641-5352474 (Clockwise)

Preceding gene: 108759220

Following gene: 108762829

Centisome position: 58.55

GC content: 68.35

Gene sequence:

>834_bases
ATGTGCCGACTATTTGGATTTCGTTCAGCGATTCCCGCCGCCGTACACCCCTCGCTGGTGACGGAGAAGAACTCGCTCCT
CATCCAGTCGCGCGAGCACAAGGATGGATGGGGCATCGCGGCCTACGGCGCCGAGCAGGCGCCGGTGGTGGCGCACGGTG
TGGGACCCGCGCACAGCGACCCGGACTTCGAGCGGGTGAGCAGCCGGGTGTCCTCCCACACGGTGGTGGCGCACATCCGC
CTGGCCTCGGTGGGCGCGGTGGAGCTGCGCAACTCGCACCCCTTCCTGCATGGCCGCTGGTCGTTCGTGCATAACGGTAC
GCTGCGGGAGTTCGCGCAGCACCGGGCTGCCGTGGAAGCGCTCATCTGCCCGAGCCTGCGGACGAACATCAGGGGCACCA
CGGACAGCGAGCGGTGCTTCTACCTGTTCCTCACCCGCTTGCATGCCCGTCATCCGATTGACCGGAAGGTCCCCGTGGAG
GCCGTGGCGCGCGCGCTGGCGGAGACGATGTCGTTGGTGGCCGCCATCACGGATGCCGTGGGACAGGATGGCCGCTCGGC
AATGAACTTCCTCGTGTCCGACGGCGAGTTGATGGTGGCCTCGCGGCGCAACCGAACGCTGTTCGTGTCCACGGGCCCGT
CGCGCGACGACGTGTCGACGCTGCCAGCGCCAGGGACGAAGCTGGAGCAGCTCGTCGTGGCCAGTGAGTCGCTGTGTGGC
GGTCCGTACTGGGCACCGGTGGCCGAAGAGGACGTCATCGGCGTGGACGCGAACCTCGTGTTCCACCACTGGCGCGTGCC
GGAGCTCGCGGGCCCGGACCTCTTCCCCGCGTGA

Upstream 100 bases:

>100_bases
CTGCGCGTGAACCGGCGTCGCAGAGACACAGCGGCTCCGAGTGGATAAAGACGGAACGACATCCGTCAGGGCCGGACGTT
AAGAATATTCGTTGCTAGCC

Downstream 100 bases:

>100_bases
AGCCGGCCACGTCACCCATCGTGGCCTGAGGCCTCGGTTGCGCGCCGAACTCATCGAACGGTGGAAGACGGCCGACGGTC
AGCGGCGGCGCTCGGAGCTC

Product: class II glutamine amidotransferase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MCRLFGFRSAIPAAVHPSLVTEKNSLLIQSREHKDGWGIAAYGAEQAPVVAHGVGPAHSDPDFERVSSRVSSHTVVAHIR
LASVGAVELRNSHPFLHGRWSFVHNGTLREFAQHRAAVEALICPSLRTNIRGTTDSERCFYLFLTRLHARHPIDRKVPVE
AVARALAETMSLVAAITDAVGQDGRSAMNFLVSDGELMVASRRNRTLFVSTGPSRDDVSTLPAPGTKLEQLVVASESLCG
GPYWAPVAEEDVIGVDANLVFHHWRVPELAGPDLFPA

Sequences:

>Translated_277_residues
MCRLFGFRSAIPAAVHPSLVTEKNSLLIQSREHKDGWGIAAYGAEQAPVVAHGVGPAHSDPDFERVSSRVSSHTVVAHIR
LASVGAVELRNSHPFLHGRWSFVHNGTLREFAQHRAAVEALICPSLRTNIRGTTDSERCFYLFLTRLHARHPIDRKVPVE
AVARALAETMSLVAAITDAVGQDGRSAMNFLVSDGELMVASRRNRTLFVSTGPSRDDVSTLPAPGTKLEQLVVASESLCG
GPYWAPVAEEDVIGVDANLVFHHWRVPELAGPDLFPA
>Mature_277_residues
MCRLFGFRSAIPAAVHPSLVTEKNSLLIQSREHKDGWGIAAYGAEQAPVVAHGVGPAHSDPDFERVSSRVSSHTVVAHIR
LASVGAVELRNSHPFLHGRWSFVHNGTLREFAQHRAAVEALICPSLRTNIRGTTDSERCFYLFLTRLHARHPIDRKVPVE
AVARALAETMSLVAAITDAVGQDGRSAMNFLVSDGELMVASRRNRTLFVSTGPSRDDVSTLPAPGTKLEQLVVASESLCG
GPYWAPVAEEDVIGVDANLVFHHWRVPELAGPDLFPA

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786417, Length=270, Percent_Identity=30, Blast_Score=81, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6324138, Length=226, Percent_Identity=26.5486725663717, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: NA

Molecular weight: Translated: 30102; Mature: 30102

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCRLFGFRSAIPAAVHPSLVTEKNSLLIQSREHKDGWGIAAYGAEQAPVVAHGVGPAHSD
CCEECCCHHCCCCCCCCHHHCCCCCEEEEECCCCCCCEEEEECCCCCCEEEECCCCCCCC
PDFERVSSRVSSHTVVAHIRLASVGAVELRNSHPFLHGRWSFVHNGTLREFAQHRAAVEA
CCHHHHHHHHHCCEEEEEEEEECCCEEEEECCCCEEEEEEEEEECCHHHHHHHHHHHHHH
LICPSLRTNIRGTTDSERCFYLFLTRLHARHPIDRKVPVEAVARALAETMSLVAAITDAV
HHCCHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GQDGRSAMNFLVSDGELMVASRRNRTLFVSTGPSRDDVSTLPAPGTKLEQLVVASESLCG
CCCCHHHHHHEECCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
GPYWAPVAEEDVIGVDANLVFHHWRVPELAGPDLFPA
CCCCCCCCCCCEEECCCEEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MCRLFGFRSAIPAAVHPSLVTEKNSLLIQSREHKDGWGIAAYGAEQAPVVAHGVGPAHSD
CCEECCCHHCCCCCCCCHHHCCCCCEEEEECCCCCCCEEEEECCCCCCEEEECCCCCCCC
PDFERVSSRVSSHTVVAHIRLASVGAVELRNSHPFLHGRWSFVHNGTLREFAQHRAAVEA
CCHHHHHHHHHCCEEEEEEEEECCCEEEEECCCCEEEEEEEEEECCHHHHHHHHHHHHHH
LICPSLRTNIRGTTDSERCFYLFLTRLHARHPIDRKVPVEAVARALAETMSLVAAITDAV
HHCCHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GQDGRSAMNFLVSDGELMVASRRNRTLFVSTGPSRDDVSTLPAPGTKLEQLVVASESLCG
CCCCHHHHHHEECCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
GPYWAPVAEEDVIGVDANLVFHHWRVPELAGPDLFPA
CCCCCCCCCCCEEECCCEEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7596361; 9278503 [H]