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The map label for this gene is trmH [C]

Identifier: 108758120

GI number: 108758120

Start: 1732525

End: 1733202

Strand: Direct

Name: trmH [C]

Synonym: MXAN_1472

Alternate gene names: 108758120

Gene position: 1732525-1733202 (Clockwise)

Preceding gene: 108759348

Following gene: 108762741

Centisome position: 18.96

GC content: 65.63

Gene sequence:

>678_bases
ATGTCGGGTGGTGGTGCGCGCTACGAGCGCTATGAGCGGGAGCAGTTCGAGCCGGAGCAGTTCCTGCTCGACGTGCGCAA
GGAGAAGATTGATCGCGTCGTCAGTCACCGGACGCGCAACTTCACGGTGGTCCTGGACCGGCTGGAGGACAGCTTCAACA
TGGCCGCGGTGCTGCGCACCTGCGAGTCCATGGGCGTTCAGGAAGTCCACATCATCATCAACCCGGAAGCGCCCTTCGTC
CCCAACTCGAGGGTGGCCCAGGGCTGCGACAAGTGGCTGGACGTGAAGCTGTACAGGACGTTCGCCGAGTGCCGCGAGCA
CCTGAAGTCCCGTGGCTTCTCGCTGTATGCCTCCGCCATTCAGGAGGGGGCCACCAGCCTCTACACCCTGCGCTTCGACG
GGAAGATGGCGCTGGTGTTCGGCAACGAGCGGCGCGGCGTGAGCGAGGACGTGCTGGCCGGCGTGGACGGCACGTTCTGG
GTCCCCATGAAGGGTTTCAGCCAGAGCCTGAACATCTCCGCCGCGGCGTCCGCCTGCATCAGCCGGGCGATTGCCTGGCG
GGACGAGCACCTGGGCCAGTCGGGGGACCTGTCTCCCGAGGATGCCCAGGCCCTGCGCGAGCGCTTCTACGTGCTGGCCA
TCAAACAGAGGAAGCGCCTGTTCAAGAAGGCCCCGTGA

Upstream 100 bases:

>100_bases
CGCAAGAGCGGCTCGTCGGGCAAGGCCGCCGCGAAGAAGGCATCCGCGGCCACGAAGCGCGCTCCGGCGCGCAAGTCGGG
CGGCGGGAGGAGCTGAGTCC

Downstream 100 bases:

>100_bases
ATGCTGGGGCTCAGGCCCCGTCGGGATGGTTTAGATGGAGGTACGCCATGTTCTTCGAAGCCCTGCTCGCTACGCTCCTG
TCCGCCCCTGCCACCCCTGC

Product: putative tRNA (guanosine-2'-O-)-methyltransferase

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MSGGGARYERYEREQFEPEQFLLDVRKEKIDRVVSHRTRNFTVVLDRLEDSFNMAAVLRTCESMGVQEVHIIINPEAPFV
PNSRVAQGCDKWLDVKLYRTFAECREHLKSRGFSLYASAIQEGATSLYTLRFDGKMALVFGNERRGVSEDVLAGVDGTFW
VPMKGFSQSLNISAAASACISRAIAWRDEHLGQSGDLSPEDAQALRERFYVLAIKQRKRLFKKAP

Sequences:

>Translated_225_residues
MSGGGARYERYEREQFEPEQFLLDVRKEKIDRVVSHRTRNFTVVLDRLEDSFNMAAVLRTCESMGVQEVHIIINPEAPFV
PNSRVAQGCDKWLDVKLYRTFAECREHLKSRGFSLYASAIQEGATSLYTLRFDGKMALVFGNERRGVSEDVLAGVDGTFW
VPMKGFSQSLNISAAASACISRAIAWRDEHLGQSGDLSPEDAQALRERFYVLAIKQRKRLFKKAP
>Mature_224_residues
SGGGARYERYEREQFEPEQFLLDVRKEKIDRVVSHRTRNFTVVLDRLEDSFNMAAVLRTCESMGVQEVHIIINPEAPFVP
NSRVAQGCDKWLDVKLYRTFAECREHLKSRGFSLYASAIQEGATSLYTLRFDGKMALVFGNERRGVSEDVLAGVDGTFWV
PMKGFSQSLNISAAASACISRAIAWRDEHLGQSGDLSPEDAQALRERFYVLAIKQRKRLFKKAP

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=159, Percent_Identity=27.6729559748428, Blast_Score=92, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 25545; Mature: 25414

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGGGARYERYEREQFEPEQFLLDVRKEKIDRVVSHRTRNFTVVLDRLEDSFNMAAVLRT
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHH
CESMGVQEVHIIINPEAPFVPNSRVAQGCDKWLDVKLYRTFAECREHLKSRGFSLYASAI
HHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
QEGATSLYTLRFDGKMALVFGNERRGVSEDVLAGVDGTFWVPMKGFSQSLNISAAASACI
HHCCCEEEEEEECCEEEEEECCCCCCCCHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHH
SRAIAWRDEHLGQSGDLSPEDAQALRERFYVLAIKQRKRLFKKAP
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SGGGARYERYEREQFEPEQFLLDVRKEKIDRVVSHRTRNFTVVLDRLEDSFNMAAVLRT
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHH
CESMGVQEVHIIINPEAPFVPNSRVAQGCDKWLDVKLYRTFAECREHLKSRGFSLYASAI
HHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
QEGATSLYTLRFDGKMALVFGNERRGVSEDVLAGVDGTFWVPMKGFSQSLNISAAASACI
HHCCCEEEEEEECCEEEEEECCCCCCCCHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHH
SRAIAWRDEHLGQSGDLSPEDAQALRERFYVLAIKQRKRLFKKAP
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]