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The map label for this gene is hoxA [H]

Identifier: 108758095

GI number: 108758095

Start: 6852295

End: 6852990

Strand: Direct

Name: hoxA [H]

Synonym: MXAN_5505

Alternate gene names: 108758095

Gene position: 6852295-6852990 (Clockwise)

Preceding gene: 108757324

Following gene: 108758978

Centisome position: 74.97

GC content: 66.95

Gene sequence:

>696_bases
ATGGATGCGTTCAAGGTTCTGGTGGTCGACGATGAGCAGCCCATCGCACGCGCGCTGCAGCGTCTGTTGAAGCGAGAGGG
ATTCGAGGTCCAGATTGCCTTCAATGGCAACGAGGCGCTGGAGCGGCTGGAAGGGTTCAGCCCGGACATCGTCCTGACGG
ATTTCCGGATGCCAGGAATGACGGGCAGCGAGCTGCTCCAGCGCCTCAAACGCAGCCATCCGCTGGCCCTGCGCCTCATC
ATTTCCGGCTATGCGGACTTCAAGTCCGTGGTGGCATCCGTGAACGAGGGCGAAATCTGCCGGTTCATCAGCAAGCCCTG
GGACGACGCGGAGCTGGTGACGTACCTCAAGGGCCTGCTGCGGCACCGCGAGACGATGGCGTGCCTCTACGCCCCCTTCC
GGCAAGCGCCGGACGGCGTGAACGCGGAGGTGGGCATGTCGGCCGCGTCCATGGTGCTCAAGGTCCAGGTGGCTGACCCG
TCCTTCCCGGCGGACCAGGCCGTTGCGCTCATCGAGCGCTTCGCGGGCCTGCTGGCCGACGACCGCCTCAAGGTGGTGGG
CGGATTGCTCGAGCGCTACGGCGGGCGCCTGTCCTTCATGGCGGACGTCGGTGGCCCCCAGCGGCTCCGGCTGGAGGTGC
CGATGCCCACCCTGGAAACCGTCAACGCCCTCCGCCCTGGCGCGAGCGACGCCTGA

Upstream 100 bases:

>100_bases
CCTGTCGCAGGTGCGAGCATGACCCGGCATTCATTTTCCAGACTCTGGGATTTTCCGTAGGTGCCCGGTCTAGAGTGCGC
GCCCGAGGAGCGACGTTGTC

Downstream 100 bases:

>100_bases
CGGAAACACACTCCACCCCACACCCAGGCACACCCTCGGCCGATAACGCACTGCGCCGTCCGATTTCACCGAAGGGAATT
TCCCCGGCCCGTTCTCACCG

Product: response regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MDAFKVLVVDDEQPIARALQRLLKREGFEVQIAFNGNEALERLEGFSPDIVLTDFRMPGMTGSELLQRLKRSHPLALRLI
ISGYADFKSVVASVNEGEICRFISKPWDDAELVTYLKGLLRHRETMACLYAPFRQAPDGVNAEVGMSAASMVLKVQVADP
SFPADQAVALIERFAGLLADDRLKVVGGLLERYGGRLSFMADVGGPQRLRLEVPMPTLETVNALRPGASDA

Sequences:

>Translated_231_residues
MDAFKVLVVDDEQPIARALQRLLKREGFEVQIAFNGNEALERLEGFSPDIVLTDFRMPGMTGSELLQRLKRSHPLALRLI
ISGYADFKSVVASVNEGEICRFISKPWDDAELVTYLKGLLRHRETMACLYAPFRQAPDGVNAEVGMSAASMVLKVQVADP
SFPADQAVALIERFAGLLADDRLKVVGGLLERYGGRLSFMADVGGPQRLRLEVPMPTLETVNALRPGASDA
>Mature_231_residues
MDAFKVLVVDDEQPIARALQRLLKREGFEVQIAFNGNEALERLEGFSPDIVLTDFRMPGMTGSELLQRLKRSHPLALRLI
ISGYADFKSVVASVNEGEICRFISKPWDDAELVTYLKGLLRHRETMACLYAPFRQAPDGVNAEVGMSAASMVLKVQVADP
SFPADQAVALIERFAGLLADDRLKVVGGLLERYGGRLSFMADVGGPQRLRLEVPMPTLETVNALRPGASDA

Specific function: Probable member of the two-component regulatory system involved in the regulation of the hydrogenase activity. HoxA is probably phosphorylated by a sensory component (which could be hoxX) and then acts in conjunction with sigma-54 as a transcriptional act

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=117, Percent_Identity=35.042735042735, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788550, Length=108, Percent_Identity=32.4074074074074, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1790437, Length=125, Percent_Identity=32, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 25388; Mature: 25388

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAFKVLVVDDEQPIARALQRLLKREGFEVQIAFNGNEALERLEGFSPDIVLTDFRMPGM
CCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCCEEEEECCCCCC
TGSELLQRLKRSHPLALRLIISGYADFKSVVASVNEGEICRFISKPWDDAELVTYLKGLL
CHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHHHH
RHRETMACLYAPFRQAPDGVNAEVGMSAASMVLKVQVADPSFPADQAVALIERFAGLLAD
HHHHHHHHHHHHHHCCCCCCCCHHCHHHEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCC
DRLKVVGGLLERYGGRLSFMADVGGPQRLRLEVPMPTLETVNALRPGASDA
CHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure
MDAFKVLVVDDEQPIARALQRLLKREGFEVQIAFNGNEALERLEGFSPDIVLTDFRMPGM
CCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCCEEEEECCCCCC
TGSELLQRLKRSHPLALRLIISGYADFKSVVASVNEGEICRFISKPWDDAELVTYLKGLL
CHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHHHH
RHRETMACLYAPFRQAPDGVNAEVGMSAASMVLKVQVADPSFPADQAVALIERFAGLLAD
HHHHHHHHHHHHHHCCCCCCCCHHCHHHEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCC
DRLKVVGGLLERYGGRLSFMADVGGPQRLRLEVPMPTLETVNALRPGASDA
CHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8510650; 12597275 [H]