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The map label for this gene is mpl [H]

Identifier: 108758004

GI number: 108758004

Start: 2242442

End: 2243866

Strand: Direct

Name: mpl [H]

Synonym: MXAN_1912

Alternate gene names: 108758004

Gene position: 2242442-2243866 (Clockwise)

Preceding gene: 108757137

Following gene: 108758314

Centisome position: 24.54

GC content: 70.88

Gene sequence:

>1425_bases
GTGCGCCGCATCCACCTGGTGGGCGTGGCGGGCACGGGCATGGGCTCCTTCGCCGGCATGCTCAAGGCCGCCGGCTACGA
CGTCACCGGCAGCGACGAGAATGTCTACCCGCCCATGAGCGACATGCTCCGGACGTGGGGCATCCCCGCCGCCACGCCGT
ACCGTCCGGAGAACCTGGACGCGGCGAAGCCGGACCTCGTCATCATCGGCAATGTCATCCGCCGCGTGAATCCCGAGGCC
ACCGCCGTGCGCGAGCGCGGCCTCAAGCAGATGAGCTTCCCCGCCGCGCTGGGCTCGCTCTTCCTGGACCGCTCGCACTC
CGTCGTCGTGGCCGGCACCCACGGGAAGACGACGACGTCCTCGCTCATGGCCCACGTACTGGTGGCGGCGGGCAAGGACC
CGTCCTTCCTCGTGGGCGGCGTCACGCAGAACTACGCGGGCAACTACCGCGTGGGGAAGGGGCCGCACTTCGTCGTCGAG
GGCGACGAGTACGACACCGCGTACTGGGACAAGGGCTCCAAGTTCCTCCACTACCGGCCGCGCACCGCCATCCTCACCAG
CGTGGAGTTCGACCACGCGGACATCTTCCGCGACTTGCCGCACTACGAGGCGACCTTCGAGAAGTTCGTCCGGCTCATCC
CCCAGGACGGGCAGCTCGTCGTCTGCGCCGCCTACCCCAACGCGGTGAAGCTGGCGCGCGAGGGTTGCCCGGGGCGCGTG
GTGACGTACGTGGCGAAGGAGGGCGCGGACGCGGACTACACGCCGCGCAACCTGTCCTTCGGCCCGGAGGGCGCGCGCTT
CGACGTGGTGGCGCGCGGCGAGGTGCAGGGCACGGTGCAGCTCCCCATGGGCGGCGCGCACAACGTGGAGAACGCGCTGG
CCGTCATCGCCGCGGCGCAGGGCCTGGGGCTCACCTTCGACGAAATCGCGCAGGGGCTGGCCACCTTCAGCGGCGTGAAG
CGCCGGCAGGAGATTCGCGGCGAGCCAGACGGCGTCCTGGTGATTGACGACTTCGCGCACCACCCGACGGCGGTGCGGGA
GACCATCGCCGCCATCCACCACCGCTACCCGGAGCGGCGGCTGTGGGCCATCTTCGAGCCGCGCTCCAACACCAGCCGCC
GCAACATCCACCAGGAGGACTACGCCCACGCCTTCCCCGGCGCGGCCCGCGCCAGCCTCAAGGTGCCCGAGCGCCACGAC
AAGGTGCCCGTGGGCGAGGAACTGGACGTGCGCAAGCTCATCACCGACCTCCAGGCCCAGGGCATTCCCGCCGAGGGCTC
CACGGACGTGCAGGCCCTGGTGGACCTGGCCGCCCGCGAGGCCCGTCCCGGCGACGTGCTGCTGGTGATGAGCAACGGGG
CCTTTGGTGGTTTCATCGACAAGGTGCTCGCGGCCCTCGCGGCCCGGGCGCAGAAGGGAGCCTGA

Upstream 100 bases:

>100_bases
CATGACTTCGTCGTGGAGGCGCTCGAGCTCACTGAAACGAAGCAGGGACACCATGGCTGACGACAACGGAAACGTCCTCG
ACACCCTCGAACTCGGCAGC

Downstream 100 bases:

>100_bases
CATGCCGCGCCTGCCATTCGCCGTCGTCGTTGGGGCGCTCGCGCTGTCCGGCTGTGCGAGCGCTCCCGCCATTCCGCCTC
TGGGGCCAGAGGGGCAGGGG

Product: UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MRRIHLVGVAGTGMGSFAGMLKAAGYDVTGSDENVYPPMSDMLRTWGIPAATPYRPENLDAAKPDLVIIGNVIRRVNPEA
TAVRERGLKQMSFPAALGSLFLDRSHSVVVAGTHGKTTTSSLMAHVLVAAGKDPSFLVGGVTQNYAGNYRVGKGPHFVVE
GDEYDTAYWDKGSKFLHYRPRTAILTSVEFDHADIFRDLPHYEATFEKFVRLIPQDGQLVVCAAYPNAVKLAREGCPGRV
VTYVAKEGADADYTPRNLSFGPEGARFDVVARGEVQGTVQLPMGGAHNVENALAVIAAAQGLGLTFDEIAQGLATFSGVK
RRQEIRGEPDGVLVIDDFAHHPTAVRETIAAIHHRYPERRLWAIFEPRSNTSRRNIHQEDYAHAFPGAARASLKVPERHD
KVPVGEELDVRKLITDLQAQGIPAEGSTDVQALVDLAAREARPGDVLLVMSNGAFGGFIDKVLAALAARAQKGA

Sequences:

>Translated_474_residues
MRRIHLVGVAGTGMGSFAGMLKAAGYDVTGSDENVYPPMSDMLRTWGIPAATPYRPENLDAAKPDLVIIGNVIRRVNPEA
TAVRERGLKQMSFPAALGSLFLDRSHSVVVAGTHGKTTTSSLMAHVLVAAGKDPSFLVGGVTQNYAGNYRVGKGPHFVVE
GDEYDTAYWDKGSKFLHYRPRTAILTSVEFDHADIFRDLPHYEATFEKFVRLIPQDGQLVVCAAYPNAVKLAREGCPGRV
VTYVAKEGADADYTPRNLSFGPEGARFDVVARGEVQGTVQLPMGGAHNVENALAVIAAAQGLGLTFDEIAQGLATFSGVK
RRQEIRGEPDGVLVIDDFAHHPTAVRETIAAIHHRYPERRLWAIFEPRSNTSRRNIHQEDYAHAFPGAARASLKVPERHD
KVPVGEELDVRKLITDLQAQGIPAEGSTDVQALVDLAAREARPGDVLLVMSNGAFGGFIDKVLAALAARAQKGA
>Mature_474_residues
MRRIHLVGVAGTGMGSFAGMLKAAGYDVTGSDENVYPPMSDMLRTWGIPAATPYRPENLDAAKPDLVIIGNVIRRVNPEA
TAVRERGLKQMSFPAALGSLFLDRSHSVVVAGTHGKTTTSSLMAHVLVAAGKDPSFLVGGVTQNYAGNYRVGKGPHFVVE
GDEYDTAYWDKGSKFLHYRPRTAILTSVEFDHADIFRDLPHYEATFEKFVRLIPQDGQLVVCAAYPNAVKLAREGCPGRV
VTYVAKEGADADYTPRNLSFGPEGARFDVVARGEVQGTVQLPMGGAHNVENALAVIAAAQGLGLTFDEIAQGLATFSGVK
RRQEIRGEPDGVLVIDDFAHHPTAVRETIAAIHHRYPERRLWAIFEPRSNTSRRNIHQEDYAHAFPGAARASLKVPERHD
KVPVGEELDVRKLITDLQAQGIPAEGSTDVQALVDLAAREARPGDVLLVMSNGAFGGFIDKVLAALAARAQKGA

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=473, Percent_Identity=39.323467230444, Blast_Score=319, Evalue=3e-88,
Organism=Escherichia coli, GI1786279, Length=402, Percent_Identity=32.3383084577114, Blast_Score=145, Evalue=7e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 51205; Mature: 51205

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRIHLVGVAGTGMGSFAGMLKAAGYDVTGSDENVYPPMSDMLRTWGIPAATPYRPENLD
CCEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCC
AAKPDLVIIGNVIRRVNPEATAVRERGLKQMSFPAALGSLFLDRSHSVVVAGTHGKTTTS
CCCCCEEEEEHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHH
SLMAHVLVAAGKDPSFLVGGVTQNYAGNYRVGKGPHFVVEGDEYDTAYWDKGSKFLHYRP
HHHHHHHHHCCCCCCEEECCCCCCCCCCEEECCCCEEEEECCCCCCHHHCCCCCEEEECC
RTAILTSVEFDHADIFRDLPHYEATFEKFVRLIPQDGQLVVCAAYPNAVKLAREGCPGRV
CEEEEEEECCCHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHCCCCCCE
VTYVAKEGADADYTPRNLSFGPEGARFDVVARGEVQGTVQLPMGGAHNVENALAVIAAAQ
EEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHC
GLGLTFDEIAQGLATFSGVKRRQEIRGEPDGVLVIDDFAHHPTAVRETIAAIHHRYPERR
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCE
LWAIFEPRSNTSRRNIHQEDYAHAFPGAARASLKVPERHDKVPVGEELDVRKLITDLQAQ
EEEEECCCCCCCCCCCCHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHC
GIPAEGSTDVQALVDLAAREARPGDVLLVMSNGAFGGFIDKVLAALAARAQKGA
CCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRRIHLVGVAGTGMGSFAGMLKAAGYDVTGSDENVYPPMSDMLRTWGIPAATPYRPENLD
CCEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCC
AAKPDLVIIGNVIRRVNPEATAVRERGLKQMSFPAALGSLFLDRSHSVVVAGTHGKTTTS
CCCCCEEEEEHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHH
SLMAHVLVAAGKDPSFLVGGVTQNYAGNYRVGKGPHFVVEGDEYDTAYWDKGSKFLHYRP
HHHHHHHHHCCCCCCEEECCCCCCCCCCEEECCCCEEEEECCCCCCHHHCCCCCEEEECC
RTAILTSVEFDHADIFRDLPHYEATFEKFVRLIPQDGQLVVCAAYPNAVKLAREGCPGRV
CEEEEEEECCCHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHCCCCCCE
VTYVAKEGADADYTPRNLSFGPEGARFDVVARGEVQGTVQLPMGGAHNVENALAVIAAAQ
EEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHC
GLGLTFDEIAQGLATFSGVKRRQEIRGEPDGVLVIDDFAHHPTAVRETIAAIHHRYPERR
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCE
LWAIFEPRSNTSRRNIHQEDYAHAFPGAARASLKVPERHDKVPVGEELDVRKLITDLQAQ
EEEEECCCCCCCCCCCCHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHC
GIPAEGSTDVQALVDLAAREARPGDVLLVMSNGAFGGFIDKVLAALAARAQKGA
CCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]