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The map label for this gene is agaA33 [H]

Identifier: 108757927

GI number: 108757927

Start: 6486062

End: 6487492

Strand: Direct

Name: agaA33 [H]

Synonym: MXAN_5194

Alternate gene names: 108757927

Gene position: 6486062-6487492 (Clockwise)

Preceding gene: 108760848

Following gene: 108761131

Centisome position: 70.97

GC content: 69.81

Gene sequence:

>1431_bases
GTGATGACCGAGCGCTTCCCGATGACGCGTCCTTCCCTGGTCACGCTGCTTCCCCTCCTCTTCTCCCTCTCCTGTGTCAG
CGGCAGCAAGATTCGCGCGGACACGGAGGTGCTCGCGGCCAACGTGGAGCGCGCCCGCCGTAGCGGCGCCCTGCGCTGCG
CGCCCGTGGAGCTGGCCACCGCCGAGGCCCACCTCGACTTCGCCCGTGGCGAGCTGAGCCAGGGCAACAGCGGCCGCGCC
GACACCCACGCGCGCACCGCCGACGCCGCGGTGACCCGCGCGCTGGAGCTCTCCAAGAACTGTGGCCCGCGCCAGGTGCT
GGTCCGCGACCGGCCGGAGGCCTCGCAGCCGCAACAGCCCCAGCAGCCGCAACAGCCGCAAGTGGTGGTCCGTATCGAGG
AGACGGACAGCGACGGCGACGGCATCCTGGACAAGGACGACCCCTGCCCCGACCAGGCCGAGGACATGGACGGGTTCCAG
GACGAGGACGGCTGCCCCGACCCGGACAACGACAACGACGGCGTGCTGGACGGCAACGACAAGTGCCCGCTCACCCCCGG
CGTGCCGGAGAACCAGGGCTGCCCGGCCGAGGCGCCCAAGGACCGCGACGGCGACGGCATCATCGACTCCGAGGACAAGT
GCCCCGACCAGGCCGAGGACAAGGACGGCTTCCAGGACGAGGACGGCTGCCCCGACCTGGACAATGACGGCGACGGCATC
GTCGACACGGCCGACAAGTGCCCCAACGAGGCCGGCCCCATGCAGAACCTGGGCTGCCCCATCGTCGACAAGGATGGTGA
CGGCATCCTCGACGTGGACGACAAGTGCCCGGACGAGCCCGAGGACAAGGACGGCTTCCAGGACGAGGACGGCTGCCCCG
ACCTGGACAATGACGGCGACGGTACGCCGGACGTCCAGGACAAGTGCCCGAACGAGGCCGGCCCGACGGAGAACGGCGGC
TGCCCGGACAAGGACACCGACGGTGACGGCATCGTGGACCGGCTGGATGCGTGCCCCGAGGAGCCGGGCGTCCAGGAGGA
GCGTGGCTGCGCCAAGCAGTACAAGATGGTCGTCATCAAGCGCGACCGGATTGAAATCAAGAAGCAGATCCTCTTCAGCT
CCGGCTCGCACAAAATCTACGGCAAGCAGAGCACCGCGGTGCTCGACGACGTGGCGCAGGCCCTGCGCGACGCGCCGTGG
ATCAAGCGCATCCGCATCGAGGGCCACACCGACTCGATGGGCAACGACGCCTCCAACCTGCGGCTGTCCCAGCGCCGCGC
GGACGCCGTCATGGCGCAGCTCCTCCGCCGCGGCATCGACCCGGGCCGCATCCAGGCCGTGGGCTTTGGCGAGACGCAGC
CCATTGCCCCCAACAGCACCAAGACGGGCCGCGCGATGAACCGCCGGACCGAGTTCAAGGTGGCGGAGTAA

Upstream 100 bases:

>100_bases
TCGACTTCGCCGTGAAGGCCTCCCGCTACGCCAACGAGGCCCGCGAGAAGGCCATGGCCGTGGCCGGAAGCACCGAGCCC
GGCGGCCGTACTCCGAACCC

Downstream 100 bases:

>100_bases
CAACCGCCCTGCCCCGGCCGCACCTGGCCGGGGCACGACGCACCAGACGCCTCCGGGGCCTCGCGCCTGCGGAGGCGTCG
TCGTTTCGGGGCTCAGCCGT

Product: OmpA domain-containing protein

Products: NA

Alternate protein names: AgaraseA33 [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MMTERFPMTRPSLVTLLPLLFSLSCVSGSKIRADTEVLAANVERARRSGALRCAPVELATAEAHLDFARGELSQGNSGRA
DTHARTADAAVTRALELSKNCGPRQVLVRDRPEASQPQQPQQPQQPQVVVRIEETDSDGDGILDKDDPCPDQAEDMDGFQ
DEDGCPDPDNDNDGVLDGNDKCPLTPGVPENQGCPAEAPKDRDGDGIIDSEDKCPDQAEDKDGFQDEDGCPDLDNDGDGI
VDTADKCPNEAGPMQNLGCPIVDKDGDGILDVDDKCPDEPEDKDGFQDEDGCPDLDNDGDGTPDVQDKCPNEAGPTENGG
CPDKDTDGDGIVDRLDACPEEPGVQEERGCAKQYKMVVIKRDRIEIKKQILFSSGSHKIYGKQSTAVLDDVAQALRDAPW
IKRIRIEGHTDSMGNDASNLRLSQRRADAVMAQLLRRGIDPGRIQAVGFGETQPIAPNSTKTGRAMNRRTEFKVAE

Sequences:

>Translated_476_residues
MMTERFPMTRPSLVTLLPLLFSLSCVSGSKIRADTEVLAANVERARRSGALRCAPVELATAEAHLDFARGELSQGNSGRA
DTHARTADAAVTRALELSKNCGPRQVLVRDRPEASQPQQPQQPQQPQVVVRIEETDSDGDGILDKDDPCPDQAEDMDGFQ
DEDGCPDPDNDNDGVLDGNDKCPLTPGVPENQGCPAEAPKDRDGDGIIDSEDKCPDQAEDKDGFQDEDGCPDLDNDGDGI
VDTADKCPNEAGPMQNLGCPIVDKDGDGILDVDDKCPDEPEDKDGFQDEDGCPDLDNDGDGTPDVQDKCPNEAGPTENGG
CPDKDTDGDGIVDRLDACPEEPGVQEERGCAKQYKMVVIKRDRIEIKKQILFSSGSHKIYGKQSTAVLDDVAQALRDAPW
IKRIRIEGHTDSMGNDASNLRLSQRRADAVMAQLLRRGIDPGRIQAVGFGETQPIAPNSTKTGRAMNRRTEFKVAE
>Mature_476_residues
MMTERFPMTRPSLVTLLPLLFSLSCVSGSKIRADTEVLAANVERARRSGALRCAPVELATAEAHLDFARGELSQGNSGRA
DTHARTADAAVTRALELSKNCGPRQVLVRDRPEASQPQQPQQPQQPQVVVRIEETDSDGDGILDKDDPCPDQAEDMDGFQ
DEDGCPDPDNDNDGVLDGNDKCPLTPGVPENQGCPAEAPKDRDGDGIIDSEDKCPDQAEDKDGFQDEDGCPDLDNDGDGI
VDTADKCPNEAGPMQNLGCPIVDKDGDGILDVDDKCPDEPEDKDGFQDEDGCPDLDNDGDGTPDVQDKCPNEAGPTENGG
CPDKDTDGDGIVDRLDACPEEPGVQEERGCAKQYKMVVIKRDRIEIKKQILFSSGSHKIYGKQSTAVLDDVAQALRDAPW
IKRIRIEGHTDSMGNDASNLRLSQRRADAVMAQLLRRGIDPGRIQAVGFGETQPIAPNSTKTGRAMNRRTEFKVAE

Specific function: Alpha-agarase. Hydrolyzes agarose, agarohexaose, neoagarohexaose and porphyran. Hydrolysis of porphyran by this enzyme improves its antioxidant activity. Does not hydrolyze kappa-carrageenan, iota-carrageenen or lambda-carrageenan [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Attached To The Membrane By A Lipid Anchor [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CBM6 (carbohydrate binding type-6) domain [H]

Homologues:

Organism=Escherichia coli, GI48994946, Length=88, Percent_Identity=43.1818181818182, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005084
- InterPro:   IPR008979
- InterPro:   IPR003367 [H]

Pfam domain/function: PF03422 CBM_6; PF02412 TSP_3 [H]

EC number: =3.2.1.158 [H]

Molecular weight: Translated: 51447; Mature: 51447

Theoretical pI: Translated: 4.12; Mature: 4.12

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTERFPMTRPSLVTLLPLLFSLSCVSGSKIRADTEVLAANVERARRSGALRCAPVELAT
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEECEEHHH
AEAHLDFARGELSQGNSGRADTHARTADAAVTRALELSKNCGPRQVLVRDRPEASQPQQP
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
QQPQQPQVVVRIEETDSDGDGILDKDDPCPDQAEDMDGFQDEDGCPDPDNDNDGVLDGND
CCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCEECCCC
KCPLTPGVPENQGCPAEAPKDRDGDGIIDSEDKCPDQAEDKDGFQDEDGCPDLDNDGDGI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
VDTADKCPNEAGPMQNLGCPIVDKDGDGILDVDDKCPDEPEDKDGFQDEDGCPDLDNDGD
EECHHHCCCCCCCHHHCCCCEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GTPDVQDKCPNEAGPTENGGCPDKDTDGDGIVDRLDACPEEPGVQEERGCAKQYKMVVIK
CCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHEEEEEE
RDRIEIKKQILFSSGSHKIYGKQSTAVLDDVAQALRDAPWIKRIRIEGHTDSMGNDASNL
CHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHH
RLSQRRADAVMAQLLRRGIDPGRIQAVGFGETQPIAPNSTKTGRAMNRRTEFKVAE
HHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHCCCCEEECC
>Mature Secondary Structure
MMTERFPMTRPSLVTLLPLLFSLSCVSGSKIRADTEVLAANVERARRSGALRCAPVELAT
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEECEEHHH
AEAHLDFARGELSQGNSGRADTHARTADAAVTRALELSKNCGPRQVLVRDRPEASQPQQP
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
QQPQQPQVVVRIEETDSDGDGILDKDDPCPDQAEDMDGFQDEDGCPDPDNDNDGVLDGND
CCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCEECCCC
KCPLTPGVPENQGCPAEAPKDRDGDGIIDSEDKCPDQAEDKDGFQDEDGCPDLDNDGDGI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
VDTADKCPNEAGPMQNLGCPIVDKDGDGILDVDDKCPDEPEDKDGFQDEDGCPDLDNDGD
EECHHHCCCCCCCHHHCCCCEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GTPDVQDKCPNEAGPTENGGCPDKDTDGDGIVDRLDACPEEPGVQEERGCAKQYKMVVIK
CCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHEEEEEE
RDRIEIKKQILFSSGSHKIYGKQSTAVLDDVAQALRDAPWIKRIRIEGHTDSMGNDASNL
CHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHH
RLSQRRADAVMAQLLRRGIDPGRIQAVGFGETQPIAPNSTKTGRAMNRRTEFKVAE
HHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA