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The map label for this gene is ansA [H]

Identifier: 108761131

GI number: 108761131

Start: 6491020

End: 6492081

Strand: Direct

Name: ansA [H]

Synonym: MXAN_5198

Alternate gene names: 108761131

Gene position: 6491020-6492081 (Clockwise)

Preceding gene: 108757927

Following gene: 108763771

Centisome position: 71.02

GC content: 70.34

Gene sequence:

>1062_bases
ATGCCCAGAGTCCTGCTGCTGCATACCGGGGGTACGCTCGGAATGGCCGGGGGGCGCCCGTCCGCCCTGAAGCCCGCGGC
CTTCTTCAAGACGCTCAAGGCCCGCGTGCCGGAGCTGTTCGAGCTGGCCGACATCGAGTTGGAGCTGTTCAGCAACCTGG
ACAGCTCGGAGATGCAGCCGGAGCTCTGGAGCCGGATGGCCGCGCACCTCCACGCGCGCCTGCCCCACTTCGACGGGGCC
GTGGTGACCCATGGCACGGACACGCTCGCCTACACCGCGAGCGCCCTCTCCTTCATGTTGCGCAACCCGCCCTGCCCCGT
GGTGTTGACGGGTTCGCAGCGGCCGTTGGGCAAGATTCGCTCAGACGCGCGGCTCAACCTCATCGACGCGGTGCTGTCCG
CGCTGCACGGGCCCCGCGAGGTCACCATCTGCTTCGACTCGCGCCTGTACCGAGGCAACCGGGCGCGCAAGGTGAAGGTG
TCCGAGTACGACGCCTTCGACAGCCCCAACTTCCCCGTGCTGGGCACCCTGGGCGTGGACACCACCTTCGAGCCGGGCCT
CAAGTCCCGCGGGGCCTTCCGCCTCTACGAGCGGTTGGACGCCAAGGTCTTCCTCCTCAAGGTGTACCCGGGGCTGGACC
CCGCGCTGCCGCTCCAGTTGCTACCGCACGTCCGGGGACTGGTGCTGGAGGCCTACGGGGCAGGTAACTTCCCCATCGCC
CCGGAACTGGGGCGCTCGCTGACGCCCCTCTTCCTCCAGGCGCGCGAGCGGGGCGTCCCCGTGGTGGTGGTGAGCCAGGC
GCACCGGAATGGCGTGGACCTTTCCTTGTATGAGTCCGGAGCGGCGGCACTGGAGGCCGGGGCGATGGGCGGCGCGGACA
TGACGCCGTCGGCGGCCCTGGTGAAGCTGATGCAGGGGCTGGCCTACCACTCCGGCTCGTCTGGGGCCCTGGCGCGCTAC
ATCCAGACCCCGGTGGCCGGAGAATTGACCGTCGGAAGGCCGACAGTCGCTCCGGAGGCCCCCGCCGGCCCCCCCGCCAC
TCGGCGGCCCCGAGGAGAGTAG

Upstream 100 bases:

>100_bases
CGACACGCTTCCAGCAGAAAAGGAGCACGCGGCGGGTCTTAGCGACGCGCGGGGGGCTTGTAAAGCAACGCCAGCCTTGG
CAGGGAAGCGCCTGTTCATC

Downstream 100 bases:

>100_bases
TGGAGAGTCGGGCAGTATGCTTGCCGCCGCGAAAAGGCGGCCCGTAAGATGGGTGCCGCGATGTCCGAGGAGAAAACTTC
CGTCCATTCGATTTCGGACC

Product: L-asparaginase

Products: NA

Alternate protein names: L-ASNase 1; L-asparagine amidohydrolase 1 [H]

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MPRVLLLHTGGTLGMAGGRPSALKPAAFFKTLKARVPELFELADIELELFSNLDSSEMQPELWSRMAAHLHARLPHFDGA
VVTHGTDTLAYTASALSFMLRNPPCPVVLTGSQRPLGKIRSDARLNLIDAVLSALHGPREVTICFDSRLYRGNRARKVKV
SEYDAFDSPNFPVLGTLGVDTTFEPGLKSRGAFRLYERLDAKVFLLKVYPGLDPALPLQLLPHVRGLVLEAYGAGNFPIA
PELGRSLTPLFLQARERGVPVVVVSQAHRNGVDLSLYESGAAALEAGAMGGADMTPSAALVKLMQGLAYHSGSSGALARY
IQTPVAGELTVGRPTVAPEAPAGPPATRRPRGE

Sequences:

>Translated_353_residues
MPRVLLLHTGGTLGMAGGRPSALKPAAFFKTLKARVPELFELADIELELFSNLDSSEMQPELWSRMAAHLHARLPHFDGA
VVTHGTDTLAYTASALSFMLRNPPCPVVLTGSQRPLGKIRSDARLNLIDAVLSALHGPREVTICFDSRLYRGNRARKVKV
SEYDAFDSPNFPVLGTLGVDTTFEPGLKSRGAFRLYERLDAKVFLLKVYPGLDPALPLQLLPHVRGLVLEAYGAGNFPIA
PELGRSLTPLFLQARERGVPVVVVSQAHRNGVDLSLYESGAAALEAGAMGGADMTPSAALVKLMQGLAYHSGSSGALARY
IQTPVAGELTVGRPTVAPEAPAGPPATRRPRGE
>Mature_352_residues
PRVLLLHTGGTLGMAGGRPSALKPAAFFKTLKARVPELFELADIELELFSNLDSSEMQPELWSRMAAHLHARLPHFDGAV
VTHGTDTLAYTASALSFMLRNPPCPVVLTGSQRPLGKIRSDARLNLIDAVLSALHGPREVTICFDSRLYRGNRARKVKVS
EYDAFDSPNFPVLGTLGVDTTFEPGLKSRGAFRLYERLDAKVFLLKVYPGLDPALPLQLLPHVRGLVLEAYGAGNFPIAP
ELGRSLTPLFLQARERGVPVVVVSQAHRNGVDLSLYESGAAALEAGAMGGADMTPSAALVKLMQGLAYHSGSSGALARYI
QTPVAGELTVGRPTVAPEAPAGPPATRRPRGE

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=331, Percent_Identity=35.9516616314199, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI1788065, Length=308, Percent_Identity=37.3376623376623, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1789327, Length=147, Percent_Identity=29.9319727891156, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI193207374, Length=365, Percent_Identity=33.1506849315069, Blast_Score=171, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI193207372, Length=365, Percent_Identity=33.1506849315069, Blast_Score=171, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI17558278, Length=365, Percent_Identity=33.1506849315069, Blast_Score=171, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI17558280, Length=365, Percent_Identity=33.1506849315069, Blast_Score=171, Evalue=7e-43,
Organism=Saccharomyces cerevisiae, GI6320527, Length=255, Percent_Identity=26.6666666666667, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323189, Length=282, Percent_Identity=26.5957446808511, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323187, Length=282, Percent_Identity=26.5957446808511, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323186, Length=282, Percent_Identity=26.5957446808511, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323184, Length=282, Percent_Identity=26.5957446808511, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24639609, Length=366, Percent_Identity=33.879781420765, Blast_Score=178, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24645530, Length=297, Percent_Identity=35.3535353535354, Blast_Score=159, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 37785; Mature: 37654

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRVLLLHTGGTLGMAGGRPSALKPAAFFKTLKARVPELFELADIELELFSNLDSSEMQP
CCCEEEEECCCEEECCCCCCCCCCHHHHHHHHHHHCCHHHHHHCCHHHHHCCCCCCCCCH
ELWSRMAAHLHARLPHFDGAVVTHGTDTLAYTASALSFMLRNPPCPVVLTGSQRPLGKIR
HHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHC
SDARLNLIDAVLSALHGPREVTICFDSRLYRGNRARKVKVSEYDAFDSPNFPVLGTLGVD
CCCHHHHHHHHHHHHCCCCEEEEEECCEEECCCCCEEEEECCCCCCCCCCCCEEEECCCC
TTFEPGLKSRGAFRLYERLDAKVFLLKVYPGLDPALPLQLLPHVRGLVLEAYGAGNFPIA
CCCCCCCCCCCHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHEEEEECCCCCCCCC
PELGRSLTPLFLQARERGVPVVVVSQAHRNGVDLSLYESGAAALEAGAMGGADMTPSAAL
HHHCCCCCHHHHHHHHCCCCEEEEECCCCCCCEEEEECCCCHHHHHCCCCCCCCCHHHHH
VKLMQGLAYHSGSSGALARYIQTPVAGELTVGRPTVAPEAPAGPPATRRPRGE
HHHHHHHHHCCCCCCHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PRVLLLHTGGTLGMAGGRPSALKPAAFFKTLKARVPELFELADIELELFSNLDSSEMQP
CCEEEEECCCEEECCCCCCCCCCHHHHHHHHHHHCCHHHHHHCCHHHHHCCCCCCCCCH
ELWSRMAAHLHARLPHFDGAVVTHGTDTLAYTASALSFMLRNPPCPVVLTGSQRPLGKIR
HHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHC
SDARLNLIDAVLSALHGPREVTICFDSRLYRGNRARKVKVSEYDAFDSPNFPVLGTLGVD
CCCHHHHHHHHHHHHCCCCEEEEEECCEEECCCCCEEEEECCCCCCCCCCCCEEEECCCC
TTFEPGLKSRGAFRLYERLDAKVFLLKVYPGLDPALPLQLLPHVRGLVLEAYGAGNFPIA
CCCCCCCCCCCHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHEEEEECCCCCCCCC
PELGRSLTPLFLQARERGVPVVVVSQAHRNGVDLSLYESGAAALEAGAMGGADMTPSAAL
HHHCCCCCHHHHHHHHCCCCEEEEECCCCCCCEEEEECCCCHHHHHCCCCCCCCCHHHHH
VKLMQGLAYHSGSSGALARYIQTPVAGELTVGRPTVAPEAPAGPPATRRPRGE
HHHHHHHHHCCCCCCHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377; 8478318 [H]