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The map label for this gene is purF [H]

Identifier: 108757527

GI number: 108757527

Start: 1284364

End: 1285800

Strand: Reverse

Name: purF [H]

Synonym: MXAN_1103

Alternate gene names: 108757527

Gene position: 1285800-1284364 (Counterclockwise)

Preceding gene: 108761592

Following gene: 108763311

Centisome position: 14.07

GC content: 68.41

Gene sequence:

>1437_bases
ATGCGTTCCTGTTCCGCTCACCGCTCGAAAGGAGTAAGGCAGCGCGCCATGTGCGGCATCTTCGGAATCGTGGGGCACGC
CGAGGCCTCCAACCTGACATACCTGGGGCTGCACGCCCTCCAGCACCGCGGGCAGGAGTCCGCGGGCATTGTCGCCTCTG
ACGGGATGGGGCTGCGCGCTCACCGGCAGATGGGACTCGTCGCCGACATCTTCGATGCGCCGGTGCTCGCCGACCTGCCC
GGGCAGGCGGCCATCGGCCACGTGCGCTACTCCACCGCTGGAGGCAGCGCCCTCAAGAACGCCCAGCCGCTCTTCGTGCA
GTACGCGGGCGGGCAGTGCGCCATCGCGCACAACGGCAACCTGGTGAACGCGGCGGAGCTGAAGCAGCAGCTCGAGGCCG
ACGGCGCCATCTTCCAGTCGGACGCGGACACGGAAGTCATCCTGCACCTGCTGGCGCGCTCCAAGCAGGCGACCTTCGAG
CAGAAGCTCGTGGAGGCGCTGCGCAAGGTGACGGGCGCCTACAGCCTCCTGGTGCTCACGGAGAACAAGCTCGTCGCGGT
GAGAGACCCGCACGGCATCCGGCCGCTGGTGCTGGGGCGGATGAAGGAAGGTGCCTACGTGCTGGCCAGCGAGTCGACCG
CGCTGGACCTCATCGAGGCGGAAACCGTGCGCGAGCTGGAGCCGGGCGAGCTACTCGTCATCGAGAACGGGCTGCTGCGC
ACCAGCAAGCCCTTCGCGGAGCCTCCGCGTCAGGCGCGCTGCATCTTCGAGCACGTGTACTTCGCGCGGCCGGACTCCAC
GCTGTTCGGCAGCAACGTGTACGAAGTGCGCAAGGAAATGGGCCGTCAGCTCGCGAAGGAGCAGCCGGCGCCCTCCGCGG
ACCTGGTCATCGCGGTGCCGGACTCGGGCGTGCCGGCGGCCATCGGCTTCGCGCAGGCCAGCGGCATTCCCTACGACGTG
GGCCTCATCCGCAGCCACTACGTGGGCCGCACCTTCATCGAGCCGCAGCAGTCCATCCGCCACTTCGGCGTGAAGCTGAA
GCTGTCCGCGGTGCGGCAGGTGCTCAAGGGCAAGCGCGTGGTGGTGGTGGACGACTCCATCGTCCGCGGCACCACGAGCC
GCAAAATCGTGAAGATGATCAAGGCGGCGGGCGCGGTGGAGGTGCACCTGCGCATCTCCTCGCCGCCCACGAAGTGGCCT
TGCTACTACGGCATCGACACGCCGAGCCGCCAGGAGCTCATCGCCGCGACGCACAGCCTGGAGGAGATTGCCACGTACGT
GACCGCAGACTCGCTCGGCTACATCTCCCAGGAAGGCCTGGGCCGCGCGGTGGGCGACCCGGAGCGCGGCAGCTTCTGCA
CCGCCTGCTTCTCCGGCAAGTACCTCATCGGCGACTTCGGCGAAGAACGCGCCGAGCCCAGCAAGCTCACCGCCTGA

Upstream 100 bases:

>100_bases
CCATGCGGGAACCAGACTAACCGCAGGGCCTGGCCTTTGGGACGCCTGACTGCCCACATGAAACGACTCTGGCGTCGGGT
GGAATACCCACCAGAGGGGC

Downstream 100 bases:

>100_bases
CGTCACCACCTGCTTCACGCCCCTGGCCCCCTGCCCGCTTCATTCCAGGCTGGGGGCCTTCGCGTTTCAGGCCGGCAAGG
CCACGGGCCGGCCCTCGATG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MRSCSAHRSKGVRQRAMCGIFGIVGHAEASNLTYLGLHALQHRGQESAGIVASDGMGLRAHRQMGLVADIFDAPVLADLP
GQAAIGHVRYSTAGGSALKNAQPLFVQYAGGQCAIAHNGNLVNAAELKQQLEADGAIFQSDADTEVILHLLARSKQATFE
QKLVEALRKVTGAYSLLVLTENKLVAVRDPHGIRPLVLGRMKEGAYVLASESTALDLIEAETVRELEPGELLVIENGLLR
TSKPFAEPPRQARCIFEHVYFARPDSTLFGSNVYEVRKEMGRQLAKEQPAPSADLVIAVPDSGVPAAIGFAQASGIPYDV
GLIRSHYVGRTFIEPQQSIRHFGVKLKLSAVRQVLKGKRVVVVDDSIVRGTTSRKIVKMIKAAGAVEVHLRISSPPTKWP
CYYGIDTPSRQELIAATHSLEEIATYVTADSLGYISQEGLGRAVGDPERGSFCTACFSGKYLIGDFGEERAEPSKLTA

Sequences:

>Translated_478_residues
MRSCSAHRSKGVRQRAMCGIFGIVGHAEASNLTYLGLHALQHRGQESAGIVASDGMGLRAHRQMGLVADIFDAPVLADLP
GQAAIGHVRYSTAGGSALKNAQPLFVQYAGGQCAIAHNGNLVNAAELKQQLEADGAIFQSDADTEVILHLLARSKQATFE
QKLVEALRKVTGAYSLLVLTENKLVAVRDPHGIRPLVLGRMKEGAYVLASESTALDLIEAETVRELEPGELLVIENGLLR
TSKPFAEPPRQARCIFEHVYFARPDSTLFGSNVYEVRKEMGRQLAKEQPAPSADLVIAVPDSGVPAAIGFAQASGIPYDV
GLIRSHYVGRTFIEPQQSIRHFGVKLKLSAVRQVLKGKRVVVVDDSIVRGTTSRKIVKMIKAAGAVEVHLRISSPPTKWP
CYYGIDTPSRQELIAATHSLEEIATYVTADSLGYISQEGLGRAVGDPERGSFCTACFSGKYLIGDFGEERAEPSKLTA
>Mature_478_residues
MRSCSAHRSKGVRQRAMCGIFGIVGHAEASNLTYLGLHALQHRGQESAGIVASDGMGLRAHRQMGLVADIFDAPVLADLP
GQAAIGHVRYSTAGGSALKNAQPLFVQYAGGQCAIAHNGNLVNAAELKQQLEADGAIFQSDADTEVILHLLARSKQATFE
QKLVEALRKVTGAYSLLVLTENKLVAVRDPHGIRPLVLGRMKEGAYVLASESTALDLIEAETVRELEPGELLVIENGLLR
TSKPFAEPPRQARCIFEHVYFARPDSTLFGSNVYEVRKEMGRQLAKEQPAPSADLVIAVPDSGVPAAIGFAQASGIPYDV
GLIRSHYVGRTFIEPQQSIRHFGVKLKLSAVRQVLKGKRVVVVDDSIVRGTTSRKIVKMIKAAGAVEVHLRISSPPTKWP
CYYGIDTPSRQELIAATHSLEEIATYVTADSLGYISQEGLGRAVGDPERGSFCTACFSGKYLIGDFGEERAEPSKLTA

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=510, Percent_Identity=39.2156862745098, Blast_Score=340, Evalue=2e-93,
Organism=Escherichia coli, GI1788651, Length=473, Percent_Identity=39.9577167019027, Blast_Score=304, Evalue=8e-84,
Organism=Escherichia coli, GI1790167, Length=205, Percent_Identity=31.7073170731707, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17554892, Length=477, Percent_Identity=42.1383647798742, Blast_Score=341, Evalue=5e-94,
Organism=Saccharomyces cerevisiae, GI6323958, Length=474, Percent_Identity=36.4978902953587, Blast_Score=262, Evalue=7e-71,
Organism=Drosophila melanogaster, GI24659598, Length=482, Percent_Identity=41.9087136929461, Blast_Score=357, Evalue=8e-99,
Organism=Drosophila melanogaster, GI24659604, Length=462, Percent_Identity=43.0735930735931, Blast_Score=354, Evalue=9e-98,
Organism=Drosophila melanogaster, GI28573187, Length=484, Percent_Identity=41.1157024793388, Blast_Score=344, Evalue=9e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 51518; Mature: 51518

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSCSAHRSKGVRQRAMCGIFGIVGHAEASNLTYLGLHALQHRGQESAGIVASDGMGLRA
CCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHCCCCCCCEEEECCCCCHH
HRQMGLVADIFDAPVLADLPGQAAIGHVRYSTAGGSALKNAQPLFVQYAGGQCAIAHNGN
HHHCCCHHHHHCCCCEECCCCCCEECEEEEECCCCCHHCCCCCEEEEECCCEEEEEECCC
LVNAAELKQQLEADGAIFQSDADTEVILHLLARSKQATFEQKLVEALRKVTGAYSLLVLT
EECHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEEEE
ENKLVAVRDPHGIRPLVLGRMKEGAYVLASESTALDLIEAETVRELEPGELLVIENGLLR
CCCEEEEECCCCCCHHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCC
TSKPFAEPPRQARCIFEHVYFARPDSTLFGSNVYEVRKEMGRQLAKEQPAPSADLVIAVP
CCCCCCCCCHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEC
DSGVPAAIGFAQASGIPYDVGLIRSHYVGRTFIEPQQSIRHFGVKLKLSAVRQVLKGKRV
CCCCCCEEEHHHHCCCCEEHHHHHHHHCCCCCCCHHHHHHHHCCEEEHHHHHHHHCCCEE
VVVDDSIVRGTTSRKIVKMIKAAGAVEVHLRISSPPTKWPCYYGIDTPSRQELIAATHSL
EEECCCHHCCCHHHHHHHHHHHCCEEEEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHH
EEIATYVTADSLGYISQEGLGRAVGDPERGSFCTACFSGKYLIGDFGEERAEPSKLTA
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEECCCHHHCCCCCCCC
>Mature Secondary Structure
MRSCSAHRSKGVRQRAMCGIFGIVGHAEASNLTYLGLHALQHRGQESAGIVASDGMGLRA
CCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHCCCCCCCEEEECCCCCHH
HRQMGLVADIFDAPVLADLPGQAAIGHVRYSTAGGSALKNAQPLFVQYAGGQCAIAHNGN
HHHCCCHHHHHCCCCEECCCCCCEECEEEEECCCCCHHCCCCCEEEEECCCEEEEEECCC
LVNAAELKQQLEADGAIFQSDADTEVILHLLARSKQATFEQKLVEALRKVTGAYSLLVLT
EECHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEEEE
ENKLVAVRDPHGIRPLVLGRMKEGAYVLASESTALDLIEAETVRELEPGELLVIENGLLR
CCCEEEEECCCCCCHHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCC
TSKPFAEPPRQARCIFEHVYFARPDSTLFGSNVYEVRKEMGRQLAKEQPAPSADLVIAVP
CCCCCCCCCHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEC
DSGVPAAIGFAQASGIPYDVGLIRSHYVGRTFIEPQQSIRHFGVKLKLSAVRQVLKGKRV
CCCCCCEEEHHHHCCCCEEHHHHHHHHCCCCCCCHHHHHHHHCCEEEHHHHHHHHCCCEE
VVVDDSIVRGTTSRKIVKMIKAAGAVEVHLRISSPPTKWPCYYGIDTPSRQELIAATHSL
EEECCCHHCCCHHHHHHHHHHHCCEEEEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHH
EEIATYVTADSLGYISQEGLGRAVGDPERGSFCTACFSGKYLIGDFGEERAEPSKLTA
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEECCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]