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The map label for this gene is livK [C]

Identifier: 108757194

GI number: 108757194

Start: 1341877

End: 1342941

Strand: Reverse

Name: livK [C]

Synonym: MXAN_1151

Alternate gene names: 108757194

Gene position: 1342941-1341877 (Counterclockwise)

Preceding gene: 108763377

Following gene: 108759240

Centisome position: 14.69

GC content: 68.83

Gene sequence:

>1065_bases
GTGGACTCGAACACGATTCTGCTGGGAGAGGTCGGCAGCCTCACGGGGAGCGAGGCGACCTTCGGGGTGTCCGCGCGCAA
CGGCATCGAGCTGGCGCTGAACGAGGCCAACGAGGCGGGCGGCGTGAAGGGCAAGAAGCTCCAGGTGCGCGTCTACGACA
GCCAGGGCCGGCCAGAGGAAGGCGCCCAGGCCGTGACGCGGCTCATCACCCAGGACAAGGTGGTGGCGATTCTCGGCGAG
GCGGCCTCGTCGGTGTCCATGGCCATGGCGGAGAAGGCGCAGGCGGGCAAGGTGCCCATGGTGACGCCCACGTCCACCGC
GCCGGAAGTGACGCAGAAGGGCGACTACATCTTCCGCGTCTGCTTCATCGACCCCTTCCAGGGGCTCGCCATGGCGAAGT
TCGCGCGGGAGAACCTGCAGCTGTCCCGCGTCGCGGTCCTGCGCGACAACAAGAGCGCGTTCTCCATGGGCCTGGCGGAC
GTGTTCACCGCCAGGTTCAAGGAGTTCGGCGGCGAGGTGCCGGGCGACGAGAGCTACTCCAAGGGCGACACGGACTTCCG
CGCGCAACTGACGTCCCTCAAGCGGCTGAAGCCGGACGCCGTCTTCGTCCCTGGCTACTACACGGACGTGGGCATCATCG
CGCGGCAGGCGCGTGAAATCGGGCTCAAGGTGCCGCTGCTCGGCGGCGACGGCTGGGACTCCGACAAGCTGTACGAGCTG
GGCGGCTCCGCGCTGGAGGGCAGCTACTTCGCGAACCACTACTCGCCGGACAACCCGGACCCGGTGCTCCAGAGGTTCCT
GGCCCGCTACAAGGCCACCTACGGCGGCGTGCCGGACAGCGTGGCCGCGCTGGCGTACGACGCGGCGCGGGTGACCATCG
ACGCCATGAAGCGCGCGCCGGACCTGAGCGGCCCTTCGCTGCGCGACGCGATTGCCGCGACGAAGGAGTTCCCGGGCGTG
ACGGGCAAAATCACCCTGGACGCCAACCGCGACGCGGTGAAGGAGGCCGTGGTGCTCAAGGTCTCCGGCGGCAAGGCGGA
GTTCGTCACCACGGTGACGCCGTAA

Upstream 100 bases:

>100_bases
GCTCGCGACCCTGGCCCTGGCCGTGGTGGCCTGCGAGAAGAAGAACGCGCCCGCCGCGCCCTCGCCGGAGGGGCAGGCGA
CCGGGGTACCGGCCGCTCCG

Downstream 100 bases:

>100_bases
CCGCGGCGCCCAGGCCCCCGTGCCTCAGATGGGGGCCTGGATGACGTAGTAGATGGAGTTGAAGTAGCGGTGCAGCGCGT
TGAGCAACTGCACCAGGAAG

Product: receptor family ligand-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MDSNTILLGEVGSLTGSEATFGVSARNGIELALNEANEAGGVKGKKLQVRVYDSQGRPEEGAQAVTRLITQDKVVAILGE
AASSVSMAMAEKAQAGKVPMVTPTSTAPEVTQKGDYIFRVCFIDPFQGLAMAKFARENLQLSRVAVLRDNKSAFSMGLAD
VFTARFKEFGGEVPGDESYSKGDTDFRAQLTSLKRLKPDAVFVPGYYTDVGIIARQAREIGLKVPLLGGDGWDSDKLYEL
GGSALEGSYFANHYSPDNPDPVLQRFLARYKATYGGVPDSVAALAYDAARVTIDAMKRAPDLSGPSLRDAIAATKEFPGV
TGKITLDANRDAVKEAVVLKVSGGKAEFVTTVTP

Sequences:

>Translated_354_residues
MDSNTILLGEVGSLTGSEATFGVSARNGIELALNEANEAGGVKGKKLQVRVYDSQGRPEEGAQAVTRLITQDKVVAILGE
AASSVSMAMAEKAQAGKVPMVTPTSTAPEVTQKGDYIFRVCFIDPFQGLAMAKFARENLQLSRVAVLRDNKSAFSMGLAD
VFTARFKEFGGEVPGDESYSKGDTDFRAQLTSLKRLKPDAVFVPGYYTDVGIIARQAREIGLKVPLLGGDGWDSDKLYEL
GGSALEGSYFANHYSPDNPDPVLQRFLARYKATYGGVPDSVAALAYDAARVTIDAMKRAPDLSGPSLRDAIAATKEFPGV
TGKITLDANRDAVKEAVVLKVSGGKAEFVTTVTP
>Mature_354_residues
MDSNTILLGEVGSLTGSEATFGVSARNGIELALNEANEAGGVKGKKLQVRVYDSQGRPEEGAQAVTRLITQDKVVAILGE
AASSVSMAMAEKAQAGKVPMVTPTSTAPEVTQKGDYIFRVCFIDPFQGLAMAKFARENLQLSRVAVLRDNKSAFSMGLAD
VFTARFKEFGGEVPGDESYSKGDTDFRAQLTSLKRLKPDAVFVPGYYTDVGIIARQAREIGLKVPLLGGDGWDSDKLYEL
GGSALEGSYFANHYSPDNPDPVLQRFLARYKATYGGVPDSVAALAYDAARVTIDAMKRAPDLSGPSLRDAIAATKEFPGV
TGKITLDANRDAVKEAVVLKVSGGKAEFVTTVTP

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=246, Percent_Identity=31.7073170731707, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI48994941, Length=243, Percent_Identity=31.6872427983539, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 37647; Mature: 37647

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSNTILLGEVGSLTGSEATFGVSARNGIELALNEANEAGGVKGKKLQVRVYDSQGRPEE
CCCCEEEEEECCCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCCHH
GAQAVTRLITQDKVVAILGEAASSVSMAMAEKAQAGKVPMVTPTSTAPEVTQKGDYIFRV
HHHHHHHHHHCCHHEEEEHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHHCCCCEEEEE
CFIDPFQGLAMAKFARENLQLSRVAVLRDNKSAFSMGLADVFTARFKEFGGEVPGDESYS
EEECCCCHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
KGDTDFRAQLTSLKRLKPDAVFVPGYYTDVGIIARQAREIGLKVPLLGGDGWDSDKLYEL
CCCHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHC
GGSALEGSYFANHYSPDNPDPVLQRFLARYKATYGGVPDSVAALAYDAARVTIDAMKRAP
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC
DLSGPSLRDAIAATKEFPGVTGKITLDANRDAVKEAVVLKVSGGKAEFVTTVTP
CCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHCEEEEEEECCCCEEEEEEECC
>Mature Secondary Structure
MDSNTILLGEVGSLTGSEATFGVSARNGIELALNEANEAGGVKGKKLQVRVYDSQGRPEE
CCCCEEEEEECCCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCCHH
GAQAVTRLITQDKVVAILGEAASSVSMAMAEKAQAGKVPMVTPTSTAPEVTQKGDYIFRV
HHHHHHHHHHCCHHEEEEHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHHCCCCEEEEE
CFIDPFQGLAMAKFARENLQLSRVAVLRDNKSAFSMGLADVFTARFKEFGGEVPGDESYS
EEECCCCHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
KGDTDFRAQLTSLKRLKPDAVFVPGYYTDVGIIARQAREIGLKVPLLGGDGWDSDKLYEL
CCCHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHC
GGSALEGSYFANHYSPDNPDPVLQRFLARYKATYGGVPDSVAALAYDAARVTIDAMKRAP
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC
DLSGPSLRDAIAATKEFPGVTGKITLDANRDAVKEAVVLKVSGGKAEFVTTVTP
CCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHCEEEEEEECCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]