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The map label for this gene is nusA [H]

Identifier: 108563823

GI number: 108563823

Start: 1452679

End: 1453866

Strand: Reverse

Name: nusA [H]

Synonym: HPAG1_1398

Alternate gene names: 108563823

Gene position: 1453866-1452679 (Counterclockwise)

Preceding gene: 108563824

Following gene: 108563822

Centisome position: 91.07

GC content: 39.9

Gene sequence:

>1188_bases
ATGGAGAAAATCAGCGATCTTATAGAATGCATTGCGTATGAAAAAAATTTGCCTAAAGAGATGATTTCAAAAGTGATTCA
AGGCTGTTTGTTAAAAATGGCGCAAAATGAGTTAGATCCCCTAGCGCGCTACTTGGTGGTTGAAGAAAACAAGCAGCTCC
AGCTTATCCAGTTGGTAGAAGTTTTAGAAGATAATGATGAAAGATTGATTAACGACCCTTCTAAATACATCAGCCTGTCT
AAAGCCAAAGAAATGGATCCAAGCGTTAAGATTAAAGACGAATTGTCTTACAGCCTGAGTTTGGAGAGCATGAAGCAAGG
AGCGATCAACCGCCTTTTTAAAGATTTGCAATACCAGTTAGAAAAAGCGTTAGAAGACAGCCACTTTGAAGCGTTTCAAA
AGCGTCTTAACAGCGTTTTAATGGGGCAAGTGATTTTAGTGGATCATAATCAAAACACTTTTATTGAGATTGAGCAGCAA
TTTCAGGGCGTTCTTTCCATGCGCCATCGCATCAAGGGCGAGAGTTTTAAAGTAGGCGATAGCATTAAAGCGGTTTTAAC
GCAAGTCAAACGCACGAAAAAAGGCTTATTATTAGAGCTGAGCCGCACCACCCCTAAAATGCTTGAAGCTCTGTTGGAAT
TAGAAGTCCCTGAAATTAAAGATAAGGAAATTGAAATCATCCATTGCGCGCGAATCCCAGGCAACAGAGCGAAAGTGAGC
TTTTTTTCCCATAACGCCAGGATTGACCCCATAGGCGCGGCTGTGGGGGTTAAGGGCGTGCGCATTAATGCGATCAGTAA
CGAATTGAATAAAGAAAACATTGATTGCATAGAGTATTCTAATGTGCCTGAAATTTATATCACTTTAGCGCTCGCTCCAG
CCAAAATTTTAAGCGTTGAGGTTAAAAAAATCCCCATAGAAGAATTGAATGCTGAAGAAAAAGAATCCGTTCAAGAGCGT
TTCATTGTCAATAACCATTTGCAAAAGGCTAAAGTGCGTTTATTAGATATTGAAAAATCTAAGGCTATCGGTAAAGGTGG
GGTGAATGTGTGCTTAGCGTCCATGCTTACAGGCTATCACATAGAGTTTGAAACCATTCCTAGCGTGAAAGAAAACGCAG
AAAATGAAAGCGAAAAAGAAACGCAAAAAGTAGGGGTAGAAGCTTTAGAGTCTTTGTTTAAGAATTGA

Upstream 100 bases:

>100_bases
AATTCGCTATAATAAAATTTCTTTTAAACGTGCCATATCCCCCACAAAACGCTAGAGAATGATAGAAAACGATAGAACAT
CAATTTAAAGGAACTTAAGA

Downstream 100 bases:

>100_bases
AATGATTTCTGTGAAACTTATTGAGTTCTTAATCAAAAGGACTTTAAGAAAATACTATGCAAGATTTACAAGATTTTAAA
AATGATATTACGCTCATTCT

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MEKISDLIECIAYEKNLPKEMISKVIQGCLLKMAQNELDPLARYLVVEENKQLQLIQLVEVLEDNDERLINDPSKYISLS
KAKEMDPSVKIKDELSYSLSLESMKQGAINRLFKDLQYQLEKALEDSHFEAFQKRLNSVLMGQVILVDHNQNTFIEIEQQ
FQGVLSMRHRIKGESFKVGDSIKAVLTQVKRTKKGLLLELSRTTPKMLEALLELEVPEIKDKEIEIIHCARIPGNRAKVS
FFSHNARIDPIGAAVGVKGVRINAISNELNKENIDCIEYSNVPEIYITLALAPAKILSVEVKKIPIEELNAEEKESVQER
FIVNNHLQKAKVRLLDIEKSKAIGKGGVNVCLASMLTGYHIEFETIPSVKENAENESEKETQKVGVEALESLFKN

Sequences:

>Translated_395_residues
MEKISDLIECIAYEKNLPKEMISKVIQGCLLKMAQNELDPLARYLVVEENKQLQLIQLVEVLEDNDERLINDPSKYISLS
KAKEMDPSVKIKDELSYSLSLESMKQGAINRLFKDLQYQLEKALEDSHFEAFQKRLNSVLMGQVILVDHNQNTFIEIEQQ
FQGVLSMRHRIKGESFKVGDSIKAVLTQVKRTKKGLLLELSRTTPKMLEALLELEVPEIKDKEIEIIHCARIPGNRAKVS
FFSHNARIDPIGAAVGVKGVRINAISNELNKENIDCIEYSNVPEIYITLALAPAKILSVEVKKIPIEELNAEEKESVQER
FIVNNHLQKAKVRLLDIEKSKAIGKGGVNVCLASMLTGYHIEFETIPSVKENAENESEKETQKVGVEALESLFKN
>Mature_395_residues
MEKISDLIECIAYEKNLPKEMISKVIQGCLLKMAQNELDPLARYLVVEENKQLQLIQLVEVLEDNDERLINDPSKYISLS
KAKEMDPSVKIKDELSYSLSLESMKQGAINRLFKDLQYQLEKALEDSHFEAFQKRLNSVLMGQVILVDHNQNTFIEIEQQ
FQGVLSMRHRIKGESFKVGDSIKAVLTQVKRTKKGLLLELSRTTPKMLEALLELEVPEIKDKEIEIIHCARIPGNRAKVS
FFSHNARIDPIGAAVGVKGVRINAISNELNKENIDCIEYSNVPEIYITLALAPAKILSVEVKKIPIEELNAEEKESVQER
FIVNNHLQKAKVRLLDIEKSKAIGKGGVNVCLASMLTGYHIEFETIPSVKENAENESEKETQKVGVEALESLFKN

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=391, Percent_Identity=25.5754475703325, Blast_Score=139, Evalue=2e-34,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N [H]

EC number: NA

Molecular weight: Translated: 44724; Mature: 44724

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKISDLIECIAYEKNLPKEMISKVIQGCLLKMAQNELDPLARYLVVEENKQLQLIQLVE
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHH
VLEDNDERLINDPSKYISLSKAKEMDPSVKIKDELSYSLSLESMKQGAINRLFKDLQYQL
HHHCCCCHHHCCHHHHHHHHHHHCCCCCEEECCCCCHHEEHHHHHHHHHHHHHHHHHHHH
EKALEDSHFEAFQKRLNSVLMGQVILVDHNQNTFIEIEQQFQGVLSMRHRIKGESFKVGD
HHHHCHHHHHHHHHHHHHHHHCEEEEEECCCCCEEEHHHHHHHHHHHHHHHCCCCEECCH
SIKAVLTQVKRTKKGLLLELSRTTPKMLEALLELEVPEIKDKEIEIIHCARIPGNRAKVS
HHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCEEE
FFSHNARIDPIGAAVGVKGVRINAISNELNKENIDCIEYSNVPEIYITLALAPAKILSVE
EEECCCCCCCCCHHHCCCCEEEEEHHHHCCCCCCCEEECCCCCEEEEEEEECCHHHHEEH
VKKIPIEELNAEEKESVQERFIVNNHLQKAKVRLLDIEKSKAIGKGGVNVCLASMLTGYH
HHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEECHHHHCCCCCHHHHHHHHHHHCCE
IEFETIPSVKENAENESEKETQKVGVEALESLFKN
EEEEECCCHHHCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEKISDLIECIAYEKNLPKEMISKVIQGCLLKMAQNELDPLARYLVVEENKQLQLIQLVE
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHH
VLEDNDERLINDPSKYISLSKAKEMDPSVKIKDELSYSLSLESMKQGAINRLFKDLQYQL
HHHCCCCHHHCCHHHHHHHHHHHCCCCCEEECCCCCHHEEHHHHHHHHHHHHHHHHHHHH
EKALEDSHFEAFQKRLNSVLMGQVILVDHNQNTFIEIEQQFQGVLSMRHRIKGESFKVGD
HHHHCHHHHHHHHHHHHHHHHCEEEEEECCCCCEEEHHHHHHHHHHHHHHHCCCCEECCH
SIKAVLTQVKRTKKGLLLELSRTTPKMLEALLELEVPEIKDKEIEIIHCARIPGNRAKVS
HHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCEEE
FFSHNARIDPIGAAVGVKGVRINAISNELNKENIDCIEYSNVPEIYITLALAPAKILSVE
EEECCCCCCCCCHHHCCCCEEEEEHHHHCCCCCCCEEECCCCCEEEEEEEECCHHHHEEH
VKKIPIEELNAEEKESVQERFIVNNHLQKAKVRLLDIEKSKAIGKGGVNVCLASMLTGYH
HHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEECHHHHCCCCCHHHHHHHHHHHCCE
IEFETIPSVKENAENESEKETQKVGVEALESLFKN
EEEEECCCHHHCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]