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The map label for this gene is exoA [H]

Identifier: 108563814

GI number: 108563814

Start: 1438897

End: 1439649

Strand: Direct

Name: exoA [H]

Synonym: HPAG1_1389

Alternate gene names: 108563814

Gene position: 1438897-1439649 (Clockwise)

Preceding gene: 108563813

Following gene: 108563817

Centisome position: 90.14

GC content: 37.72

Gene sequence:

>753_bases
ATGAAACTGATTTCATGGAATGTGAACGGGTTAAGGGCTTGCATGACTAAGGGTTTTATGGATTTTTTCAATAGCGTTGA
TGCAGATGTTTTTTGCATTCAAGAATCTAAAATGCAGCAAGAACAAAACACCTTTGAATTTAGAGGATATTTTGATTTTT
GGAATTGCGCGATTAAAAAGGGCTATTCTGGGGTGGTAACTTTCACTAAAAAAGAGCCTTTAAGCGTAAGCTATGGTATT
AATATTGAAGAGCATGACAAAGAGGGGCGTGTGGTAACTTGCGAATTTGAGTCCTTTTATTTAGTGAATGTTTATACCCC
TAATTCCCAACAAGCCCTATCCAGGCTTAGTTATCGCATGAGTTGGGAAGTGGAATTTAAAAAATTTTTAAAAGCTTTAG
AATTGAAAAAACCGGTCATTGTGTGTGGGGATTTGAATGTGGCCCACAATGAAATTGATTTAGAAAACCCCAAAACAAAC
CGAAAAAACGCGGGCTTTAGCGATGAAGAGAGAGGGAAATTCAGCGAGCTTTTGAACGCCGGCTTTATTGACACTTTCCG
TTATTTTTACCCCAACAAAGAAAAGGCTTACACCTGGTGGAGTTATATGCAACAAGCAAGGGATAAAAACATTGGTTGGC
GCATTGATTATTTTCTATGCTCTAATCCTTTAAAAACGCGCTTAAAAGACGCTTTAATCTATAAAGATATTTTAGGGAGC
GATCATTGCCCGGTGGGGTTGGAATTAGTTTAA

Upstream 100 bases:

>100_bases
AGCAAAAAACAATTATTACATAAAAAAGGTAAAATAAAGCTTAATTTTTAAAAGAATGACTAAAAAGATATAATTTATTC
AAAAGGGAACAAGGATTAAA

Downstream 100 bases:

>100_bases
AGGTAGAAAGTGTGCGAAATAAAGACAGAAAAAAGCCTTACAAAGGGTGAGTCGTATTCAAAAGCGAAAGGCCATTTGAT
CCCCACTTCAATACTAGCGT

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MKLISWNVNGLRACMTKGFMDFFNSVDADVFCIQESKMQQEQNTFEFRGYFDFWNCAIKKGYSGVVTFTKKEPLSVSYGI
NIEEHDKEGRVVTCEFESFYLVNVYTPNSQQALSRLSYRMSWEVEFKKFLKALELKKPVIVCGDLNVAHNEIDLENPKTN
RKNAGFSDEERGKFSELLNAGFIDTFRYFYPNKEKAYTWWSYMQQARDKNIGWRIDYFLCSNPLKTRLKDALIYKDILGS
DHCPVGLELV

Sequences:

>Translated_250_residues
MKLISWNVNGLRACMTKGFMDFFNSVDADVFCIQESKMQQEQNTFEFRGYFDFWNCAIKKGYSGVVTFTKKEPLSVSYGI
NIEEHDKEGRVVTCEFESFYLVNVYTPNSQQALSRLSYRMSWEVEFKKFLKALELKKPVIVCGDLNVAHNEIDLENPKTN
RKNAGFSDEERGKFSELLNAGFIDTFRYFYPNKEKAYTWWSYMQQARDKNIGWRIDYFLCSNPLKTRLKDALIYKDILGS
DHCPVGLELV
>Mature_250_residues
MKLISWNVNGLRACMTKGFMDFFNSVDADVFCIQESKMQQEQNTFEFRGYFDFWNCAIKKGYSGVVTFTKKEPLSVSYGI
NIEEHDKEGRVVTCEFESFYLVNVYTPNSQQALSRLSYRMSWEVEFKKFLKALELKKPVIVCGDLNVAHNEIDLENPKTN
RKNAGFSDEERGKFSELLNAGFIDTFRYFYPNKEKAYTWWSYMQQARDKNIGWRIDYFLCSNPLKTRLKDALIYKDILGS
DHCPVGLELV

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=256, Percent_Identity=49.609375, Blast_Score=262, Evalue=2e-70,
Organism=Homo sapiens, GI18375503, Length=256, Percent_Identity=49.609375, Blast_Score=262, Evalue=2e-70,
Organism=Homo sapiens, GI18375501, Length=256, Percent_Identity=49.609375, Blast_Score=262, Evalue=2e-70,
Organism=Homo sapiens, GI18375507, Length=317, Percent_Identity=27.4447949526814, Blast_Score=107, Evalue=7e-24,
Organism=Escherichia coli, GI1788046, Length=266, Percent_Identity=28.9473684210526, Blast_Score=104, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI71989536, Length=253, Percent_Identity=42.2924901185771, Blast_Score=208, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI71989539, Length=143, Percent_Identity=34.2657342657343, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI221330655, Length=250, Percent_Identity=47.6, Blast_Score=258, Evalue=3e-69,
Organism=Drosophila melanogaster, GI17136678, Length=250, Percent_Identity=47.6, Blast_Score=258, Evalue=3e-69,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29250; Mature: 29250

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLISWNVNGLRACMTKGFMDFFNSVDADVFCIQESKMQQEQNTFEFRGYFDFWNCAIKK
CEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHCCCEEEEEEHHHHHHHHHC
GYSGVVTFTKKEPLSVSYGINIEEHDKEGRVVTCEFESFYLVNVYTPNSQQALSRLSYRM
CCCEEEEEECCCCCEEEECCCCCCCCCCCCEEEEEECCEEEEEEECCCHHHHHHHHHHHE
SWEVEFKKFLKALELKKPVIVCGDLNVAHNEIDLENPKTNRKNAGFSDEERGKFSELLNA
EEEEHHHHHHHHHHCCCCEEEEECCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHC
GFIDTFRYFYPNKEKAYTWWSYMQQARDKNIGWRIDYFLCSNPLKTRLKDALIYKDILGS
CHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHCC
DHCPVGLELV
CCCCCCCEEC
>Mature Secondary Structure
MKLISWNVNGLRACMTKGFMDFFNSVDADVFCIQESKMQQEQNTFEFRGYFDFWNCAIKK
CEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHCCCEEEEEEHHHHHHHHHC
GYSGVVTFTKKEPLSVSYGINIEEHDKEGRVVTCEFESFYLVNVYTPNSQQALSRLSYRM
CCCEEEEEECCCCCEEEECCCCCCCCCCCCEEEEEECCEEEEEEECCCHHHHHHHHHHHE
SWEVEFKKFLKALELKKPVIVCGDLNVAHNEIDLENPKTNRKNAGFSDEERGKFSELLNA
EEEEHHHHHHHHHHCCCCEEEEECCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHC
GFIDTFRYFYPNKEKAYTWWSYMQQARDKNIGWRIDYFLCSNPLKTRLKDALIYKDILGS
CHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHCC
DHCPVGLELV
CCCCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]