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The map label for this gene is trmE

Identifier: 108563803

GI number: 108563803

Start: 1427104

End: 1428489

Strand: Direct

Name: trmE

Synonym: HPAG1_1378

Alternate gene names: 108563803

Gene position: 1427104-1428489 (Clockwise)

Preceding gene: 108563802

Following gene: 108563804

Centisome position: 89.4

GC content: 39.47

Gene sequence:

>1386_bases
ATGAAAAATACATCGTCATCAACGACTTTAACAATGAATGACACCATCGCCGCTATCGCTACCCCTTCAGGCAAGGGAGC
GATTAGCATCATTAAAATCAGCGGCCATAACGCCCTAAACATCCTCAAACAACTCACAAAAAAACAAGACTTCACCCCCA
GATACGCTTACGTGCGCGATATTTTTTCTGATGGTGTTTTATTGGACAAAGCGTTAGTGATTTATTTCAAAGCCCCCTAT
AGTTTCACCGGTGAAGATGTGTGCGAAATCCAATGCCATGGAAGCCCCCTTTTAGCACAAAATATCTTACAAGCTTGTTT
GAATTTAGGGGCTAGGCTTGCTAAAGCGGGGGAATTTAGCAAAAAAGCCTTTTTAAACCATAAAATGGATTTGAGCGAGA
TTGAAGCGAGCGTTCAGCTCATCCTTTGTGAAGATGAAAGCGTTTTAAACGCTCTAGCCAGGCAGCTTAAGGGCGAGTTA
AAAATTTTTATTGAAGAGGCTAGGAGCGATCTTTTAAAGCTTTTGGCCAGCTCAGAAGTTTTGATTGATTATAGCGAAGA
AGACATTCCTAGCGATTTTTTAAAGGAAGTGTCTTTTAATTTAGAAAAACAAATCGCCTCTTTTAAAGATTTATTGGATT
TTTCTAACGCGCAAAAACAAAAAAACAAAGGGCATGCCTTAAGCATTGTTGGCAAACCCAATGCCGGCAAAAGCTCCCTA
TTAAACGCCATGCTGTTAGAAGAAAGGGCTTTGGTGAGCGACATTAAAGGCACGACAAGAGACACTATAGAAGAAGTCAT
TGAACTAAAAGGGCATAAGGTGCGCTTGATTGATACGGCCGGTATTAGAGAGAGCGCGGATAAAATAGAGCGTTTAGGGA
TTGAAAAAAGCCTTAAAAGTTTAGAAAATTGCGACATTATTTTAGGCGTGTTTGATCTTTCTAAACCCCTAGAAAAAGAA
GATTTTACTATCATTGATGCCCTTAATCGCGCTAAAAAGCCTTGCATTGTTGTTTTGAATAAAAACGATTTAGCCCCTAA
ACTAGAGCTTGAAATTTTAAAATCCCATCTTAAAATCCCTTATTCTATCCTAGAGACCAACACCTTAAATTCTAAGGCTT
GTTTGAAAGATTTGGGCCAAAAAATCAGCGCCTTTTTCCCCAAACTAGACACTCAAAACAAGCTTTTACTCACTTCCCTA
GCCCAAAAAACCGCCCTAGAAAACGCCATTACTGAATTGCAAAACGCTAAAAACCATTTAGAAACTTTGGAGCTTTTTTC
TTATCATCTTTTAAGCGCGATAGAAAACTTGAACTCGCTCACCCGCCCTTATGAAACCAGCCAAATGCTAGACAGCATGT
TCAGCGAATTTTGCTTAGGCAAATGA

Upstream 100 bases:

>100_bases
CAGATGAAAGCCCCTGATGGCGTTCTAACCTATATCGCTTTAAAGAAATTACGAAAAGCGTTCCCTAATAAATATGTCTC
TATCAAAACCAATTTAAACG

Downstream 100 bases:

>100_bases
AACCGCTTAAAGAATGGGTTAAAACCCTTTTTAGCGCTACTTTTTAAGACTTTTTTACCCATTAGTGCGGTCTAAGATCC
ATTTTTACAAAAAACAATAC

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MKNTSSSTTLTMNDTIAAIATPSGKGAISIIKISGHNALNILKQLTKKQDFTPRYAYVRDIFSDGVLLDKALVIYFKAPY
SFTGEDVCEIQCHGSPLLAQNILQACLNLGARLAKAGEFSKKAFLNHKMDLSEIEASVQLILCEDESVLNALARQLKGEL
KIFIEEARSDLLKLLASSEVLIDYSEEDIPSDFLKEVSFNLEKQIASFKDLLDFSNAQKQKNKGHALSIVGKPNAGKSSL
LNAMLLEERALVSDIKGTTRDTIEEVIELKGHKVRLIDTAGIRESADKIERLGIEKSLKSLENCDIILGVFDLSKPLEKE
DFTIIDALNRAKKPCIVVLNKNDLAPKLELEILKSHLKIPYSILETNTLNSKACLKDLGQKISAFFPKLDTQNKLLLTSL
AQKTALENAITELQNAKNHLETLELFSYHLLSAIENLNSLTRPYETSQMLDSMFSEFCLGK

Sequences:

>Translated_461_residues
MKNTSSSTTLTMNDTIAAIATPSGKGAISIIKISGHNALNILKQLTKKQDFTPRYAYVRDIFSDGVLLDKALVIYFKAPY
SFTGEDVCEIQCHGSPLLAQNILQACLNLGARLAKAGEFSKKAFLNHKMDLSEIEASVQLILCEDESVLNALARQLKGEL
KIFIEEARSDLLKLLASSEVLIDYSEEDIPSDFLKEVSFNLEKQIASFKDLLDFSNAQKQKNKGHALSIVGKPNAGKSSL
LNAMLLEERALVSDIKGTTRDTIEEVIELKGHKVRLIDTAGIRESADKIERLGIEKSLKSLENCDIILGVFDLSKPLEKE
DFTIIDALNRAKKPCIVVLNKNDLAPKLELEILKSHLKIPYSILETNTLNSKACLKDLGQKISAFFPKLDTQNKLLLTSL
AQKTALENAITELQNAKNHLETLELFSYHLLSAIENLNSLTRPYETSQMLDSMFSEFCLGK
>Mature_461_residues
MKNTSSSTTLTMNDTIAAIATPSGKGAISIIKISGHNALNILKQLTKKQDFTPRYAYVRDIFSDGVLLDKALVIYFKAPY
SFTGEDVCEIQCHGSPLLAQNILQACLNLGARLAKAGEFSKKAFLNHKMDLSEIEASVQLILCEDESVLNALARQLKGEL
KIFIEEARSDLLKLLASSEVLIDYSEEDIPSDFLKEVSFNLEKQIASFKDLLDFSNAQKQKNKGHALSIVGKPNAGKSSL
LNAMLLEERALVSDIKGTTRDTIEEVIELKGHKVRLIDTAGIRESADKIERLGIEKSLKSLENCDIILGVFDLSKPLEKE
DFTIIDALNRAKKPCIVVLNKNDLAPKLELEILKSHLKIPYSILETNTLNSKACLKDLGQKISAFFPKLDTQNKLLLTSL
AQKTALENAITELQNAKNHLETLELFSYHLLSAIENLNSLTRPYETSQMLDSMFSEFCLGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=479, Percent_Identity=32.776617954071, Blast_Score=205, Evalue=6e-53,
Organism=Homo sapiens, GI193082988, Length=511, Percent_Identity=30.7240704500978, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI193082993, Length=476, Percent_Identity=31.7226890756302, Blast_Score=191, Evalue=1e-48,
Organism=Escherichia coli, GI2367268, Length=474, Percent_Identity=34.1772151898734, Blast_Score=233, Evalue=3e-62,
Organism=Escherichia coli, GI87082120, Length=120, Percent_Identity=36.6666666666667, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17507259, Length=461, Percent_Identity=29.7180043383948, Blast_Score=192, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6323665, Length=490, Percent_Identity=32.6530612244898, Blast_Score=205, Evalue=1e-53,
Organism=Drosophila melanogaster, GI45550813, Length=304, Percent_Identity=32.8947368421053, Blast_Score=155, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_HELPH (Q1CRH7)

Other databases:

- EMBL:   CP000241
- RefSeq:   YP_628119.1
- ProteinModelPortal:   Q1CRH7
- SMR:   Q1CRH7
- STRING:   Q1CRH7
- GeneID:   4098049
- GenomeReviews:   CP000241_GR
- KEGG:   hpa:HPAG1_1378
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- BioCyc:   HPYL357544:HPAG1_1378-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 51136; Mature: 51136

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: BINDING 32-32 BINDING 89-89 BINDING 128-128 BINDING 461-461

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNTSSSTTLTMNDTIAAIATPSGKGAISIIKISGHNALNILKQLTKKQDFTPRYAYVRD
CCCCCCCEEEEECCCEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHH
IFSDGVLLDKALVIYFKAPYSFTGEDVCEIQCHGSPLLAQNILQACLNLGARLAKAGEFS
HHHCCHHHHCEEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHH
KKAFLNHKMDLSEIEASVQLILCEDESVLNALARQLKGELKIFIEEARSDLLKLLASSEV
HHHHHHCCCCHHHHHCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
LIDYSEEDIPSDFLKEVSFNLEKQIASFKDLLDFSNAQKQKNKGHALSIVGKPNAGKSSL
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHH
LNAMLLEERALVSDIKGTTRDTIEEVIELKGHKVRLIDTAGIRESADKIERLGIEKSLKS
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
LENCDIILGVFDLSKPLEKEDFTIIDALNRAKKPCIVVLNKNDLAPKLELEILKSHLKIP
HCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCC
YSILETNTLNSKACLKDLGQKISAFFPKLDTQNKLLLTSLAQKTALENAITELQNAKNHL
HHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETLELFSYHLLSAIENLNSLTRPYETSQMLDSMFSEFCLGK
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKNTSSSTTLTMNDTIAAIATPSGKGAISIIKISGHNALNILKQLTKKQDFTPRYAYVRD
CCCCCCCEEEEECCCEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHH
IFSDGVLLDKALVIYFKAPYSFTGEDVCEIQCHGSPLLAQNILQACLNLGARLAKAGEFS
HHHCCHHHHCEEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHH
KKAFLNHKMDLSEIEASVQLILCEDESVLNALARQLKGELKIFIEEARSDLLKLLASSEV
HHHHHHCCCCHHHHHCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
LIDYSEEDIPSDFLKEVSFNLEKQIASFKDLLDFSNAQKQKNKGHALSIVGKPNAGKSSL
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHH
LNAMLLEERALVSDIKGTTRDTIEEVIELKGHKVRLIDTAGIRESADKIERLGIEKSLKS
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
LENCDIILGVFDLSKPLEKEDFTIIDALNRAKKPCIVVLNKNDLAPKLELEILKSHLKIP
HCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCC
YSILETNTLNSKACLKDLGQKISAFFPKLDTQNKLLLTSLAQKTALENAITELQNAKNHL
HHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETLELFSYHLLSAIENLNSLTRPYETSQMLDSMFSEFCLGK
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA