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The map label for this gene is thiD [H]

Identifier: 108563254

GI number: 108563254

Start: 863228

End: 864037

Strand: Reverse

Name: thiD [H]

Synonym: HPAG1_0829

Alternate gene names: 108563254

Gene position: 864037-863228 (Counterclockwise)

Preceding gene: 108563255

Following gene: 108563253

Centisome position: 54.13

GC content: 43.09

Gene sequence:

>810_bases
GTGAAAATTTACCCGCAAGTTTTAAGCATTGCTGGCAGCGATAGCGGTGGGGGCGCTGGGATACAAGCCGATTTGAAAGC
GTTCCAAACTTTGGGTGTGTTTGGGACAAGCGTGATCACTTGCATCACCGCGCAAAACACACAGGGCGTGCATGGGGTTT
ATCCGTTGAGCGTTGAGAGCGTGAAAGCGCAAATCTTAGCCATTAGAGATGATTTTTCTATCAAAGCGTTCAAAATAGGG
GCTTTATGCAACAATCAAATCATTGAATGCGTAGCGGACACTTTAGAAACTTGTGATTTTGGGTTGTGCGTTTTAGATCC
GGTAATGGTGGCAAAGAATGGGGCTTTGCTTTTAGAAGAAGAGGCGATTTTAAGCTTAAAAAAACGCCTTTTACCTAAAA
CCCATTTACTAACCCCTAACCTCCCTGAAGTTTATGCGCTCACAGGCGTTCAAGTGCGAGATGATAAAAGCGCTTCAAAA
GCGATGGGCGTTTTAAGGGATTTAGGCGTTAAAAACGCTGTGATTAAAGGGGGGCATACAGAGCGTTTTCAAGGGGAATT
TAGCAACGACTGGGTGTTTTTAGAAGATATTGAATTTATCTTAAACGCCAAGCGCTTTAACACGAAAAACACGCATGGCA
CGGGCTGCACTCTTTCTAGCTTGATTGTGGGCTTATTCGCTCAAGGGTTGGATTTAAAAAACGCTATTTCAAAGGCTAAA
GAGCTTTTAACTACCATCATTCAAAACCCCTTAAACATTGGGCATGGGCATGGGCCTTTGAATTTATGGAGTATTAAAAA
TCTTGTTTGA

Upstream 100 bases:

>100_bases
CGTTGGAAAATAACGGCTCTAATGGTTCGTTCTTGTTTTATTTTTTAGACGCTCTGAGCTTGCCCATAAAGTTAGAAAAT
AGCCTTATTAAGGAAGTATT

Downstream 100 bases:

>100_bases
TGCGAATTGTTTGAAACTCATGTTTGTGGCTGGCTCGCAAGACTTCTACCACATAAAGGGCGGCAAAAACGATAGGATAA
ACGCACTTTTAGACACTTTA

Product: thiamine biosynthesis protein

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MKIYPQVLSIAGSDSGGGAGIQADLKAFQTLGVFGTSVITCITAQNTQGVHGVYPLSVESVKAQILAIRDDFSIKAFKIG
ALCNNQIIECVADTLETCDFGLCVLDPVMVAKNGALLLEEEAILSLKKRLLPKTHLLTPNLPEVYALTGVQVRDDKSASK
AMGVLRDLGVKNAVIKGGHTERFQGEFSNDWVFLEDIEFILNAKRFNTKNTHGTGCTLSSLIVGLFAQGLDLKNAISKAK
ELLTTIIQNPLNIGHGHGPLNLWSIKNLV

Sequences:

>Translated_269_residues
MKIYPQVLSIAGSDSGGGAGIQADLKAFQTLGVFGTSVITCITAQNTQGVHGVYPLSVESVKAQILAIRDDFSIKAFKIG
ALCNNQIIECVADTLETCDFGLCVLDPVMVAKNGALLLEEEAILSLKKRLLPKTHLLTPNLPEVYALTGVQVRDDKSASK
AMGVLRDLGVKNAVIKGGHTERFQGEFSNDWVFLEDIEFILNAKRFNTKNTHGTGCTLSSLIVGLFAQGLDLKNAISKAK
ELLTTIIQNPLNIGHGHGPLNLWSIKNLV
>Mature_269_residues
MKIYPQVLSIAGSDSGGGAGIQADLKAFQTLGVFGTSVITCITAQNTQGVHGVYPLSVESVKAQILAIRDDFSIKAFKIG
ALCNNQIIECVADTLETCDFGLCVLDPVMVAKNGALLLEEEAILSLKKRLLPKTHLLTPNLPEVYALTGVQVRDDKSASK
AMGVLRDLGVKNAVIKGGHTERFQGEFSNDWVFLEDIEFILNAKRFNTKNTHGTGCTLSSLIVGLFAQGLDLKNAISKAK
ELLTTIIQNPLNIGHGHGPLNLWSIKNLV

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=257, Percent_Identity=41.6342412451362, Blast_Score=191, Evalue=5e-50,
Organism=Saccharomyces cerevisiae, GI6325378, Length=263, Percent_Identity=30.0380228136882, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324997, Length=281, Percent_Identity=33.0960854092527, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324517, Length=279, Percent_Identity=30.8243727598566, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 28914; Mature: 28914

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYPQVLSIAGSDSGGGAGIQADLKAFQTLGVFGTSVITCITAQNTQGVHGVYPLSVES
CCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCEECCCHHH
VKAQILAIRDDFSIKAFKIGALCNNQIIECVADTLETCDFGLCVLDPVMVAKNGALLLEE
HHEEEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEH
EAILSLKKRLLPKTHLLTPNLPEVYALTGVQVRDDKSASKAMGVLRDLGVKNAVIKGGHT
HHHHHHHHHHCCCHHCCCCCCCCCEEECCEEEECCCHHHHHHHHHHHCCCCHHEECCCCC
ERFQGEFSNDWVFLEDIEFILNAKRFNTKNTHGTGCTLSSLIVGLFAQGLDLKNAISKAK
HHCCCCCCCCEEEHEEHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHH
ELLTTIIQNPLNIGHGHGPLNLWSIKNLV
HHHHHHHHCCCCCCCCCCCEEEHHHHHCC
>Mature Secondary Structure
MKIYPQVLSIAGSDSGGGAGIQADLKAFQTLGVFGTSVITCITAQNTQGVHGVYPLSVES
CCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCEECCCHHH
VKAQILAIRDDFSIKAFKIGALCNNQIIECVADTLETCDFGLCVLDPVMVAKNGALLLEE
HHEEEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEH
EAILSLKKRLLPKTHLLTPNLPEVYALTGVQVRDDKSASKAMGVLRDLGVKNAVIKGGHT
HHHHHHHHHHCCCHHCCCCCCCCCEEECCEEEECCCHHHHHHHHHHHCCCCHHEECCCCC
ERFQGEFSNDWVFLEDIEFILNAKRFNTKNTHGTGCTLSSLIVGLFAQGLDLKNAISKAK
HHCCCCCCCCEEEHEEHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHH
ELLTTIIQNPLNIGHGHGPLNLWSIKNLV
HHHHHHHHCCCCCCCCCCCEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9252185 [H]