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The map label for this gene is thiE

Identifier: 108563253

GI number: 108563253

Start: 862576

End: 863235

Strand: Reverse

Name: thiE

Synonym: HPAG1_0828

Alternate gene names: 108563253

Gene position: 863235-862576 (Counterclockwise)

Preceding gene: 108563254

Following gene: 108563244

Centisome position: 54.08

GC content: 42.27

Gene sequence:

>660_bases
TTGTTTGATGCGAATTGTTTGAAACTCATGTTTGTGGCTGGCTCGCAAGACTTCTACCACATAAAGGGCGGCAAAAACGA
TAGGATAAACGCACTTTTAGACACTTTAGAATTAGCTTTACAATCTCAAATCACAGCGTTTCAATTCCGCCAAAAAGGCG
ATTTAGCCTTACAAGATCCTGTTGAAATCAAACAATTAGCCCTGAAGTGTCAAAAATTATGCCAAAAATACGGCACGCCT
TTTATTATTAATGATGAGGTGCGACTCGCGCTAGAATTAAAGGCTGATGGCGTGCATGTGGGGCAAGAAGACATGGCTAT
AGAAGAGGTGATAGCTTTATGCAAAAAGCACCAATTTATAGGTTTGAGCGTCAATACTTTAGAGCAAGCCCTAAAAGCGC
GCCATTTAGACGCCGTAGCCTATTTTGGGGTAGGCCCTATTTTCCCCACACCATCTAAAAAAGACAAACAAGTTGTAGGC
GTAGAGCTTTTAAAAAAAATACGAGACAGCGGGGTAAAAAAACCCCTCATAGCGATTGGGGGCATCACGACGCATAACGC
TTCAAAATTGCGCGAATATGGGGGTATCGCAGTCATTAGCGCGATCACGCAAGCCAGAGATAAAGCCTTAGCAATTGAAA
AACTTTTAAACAATGCGTGA

Upstream 100 bases:

>100_bases
GCTATTTCAAAGGCTAAAGAGCTTTTAACTACCATCATTCAAAACCCCTTAAACATTGGGCATGGGCATGGGCCTTTGAA
TTTATGGAGTATTAAAAATC

Downstream 100 bases:

>100_bases
GGGATTCTAAAATCCCACAAAAAGCGTTTTTTAAAAAAATCAAGTTTTAGTTTTCTACCCTATCGTGTCCAATAAACGCT
CTTCAAAGGCAAAAAGCATG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MFDANCLKLMFVAGSQDFYHIKGGKNDRINALLDTLELALQSQITAFQFRQKGDLALQDPVEIKQLALKCQKLCQKYGTP
FIINDEVRLALELKADGVHVGQEDMAIEEVIALCKKHQFIGLSVNTLEQALKARHLDAVAYFGVGPIFPTPSKKDKQVVG
VELLKKIRDSGVKKPLIAIGGITTHNASKLREYGGIAVISAITQARDKALAIEKLLNNA

Sequences:

>Translated_219_residues
MFDANCLKLMFVAGSQDFYHIKGGKNDRINALLDTLELALQSQITAFQFRQKGDLALQDPVEIKQLALKCQKLCQKYGTP
FIINDEVRLALELKADGVHVGQEDMAIEEVIALCKKHQFIGLSVNTLEQALKARHLDAVAYFGVGPIFPTPSKKDKQVVG
VELLKKIRDSGVKKPLIAIGGITTHNASKLREYGGIAVISAITQARDKALAIEKLLNNA
>Mature_219_residues
MFDANCLKLMFVAGSQDFYHIKGGKNDRINALLDTLELALQSQITAFQFRQKGDLALQDPVEIKQLALKCQKLCQKYGTP
FIINDEVRLALELKADGVHVGQEDMAIEEVIALCKKHQFIGLSVNTLEQALKARHLDAVAYFGVGPIFPTPSKKDKQVVG
VELLKKIRDSGVKKPLIAIGGITTHNASKLREYGGIAVISAITQARDKALAIEKLLNNA

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=164, Percent_Identity=34.1463414634146, Blast_Score=75, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6325042, Length=209, Percent_Identity=31.5789473684211, Blast_Score=92, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_HELPH (Q1CT27)

Other databases:

- EMBL:   CP000241
- RefSeq:   YP_627569.1
- ProteinModelPortal:   Q1CT27
- SMR:   Q1CT27
- STRING:   Q1CT27
- GeneID:   4098194
- GenomeReviews:   CP000241_GR
- KEGG:   hpa:HPAG1_0828
- eggNOG:   COG0352
- HOGENOM:   HBG754477
- OMA:   CERYKTR
- ProtClustDB:   PRK00043
- BioCyc:   HPYL357544:HPAG1_0828-MONOMER
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 24052; Mature: 24052

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDANCLKLMFVAGSQDFYHIKGGKNDRINALLDTLELALQSQITAFQFRQKGDLALQDP
CCCCCEEEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
VEIKQLALKCQKLCQKYGTPFIINDEVRLALELKADGVHVGQEDMAIEEVIALCKKHQFI
HHHHHHHHHHHHHHHHCCCCEEECCCEEEEEEEECCCEEECHHHHHHHHHHHHHHCCCEE
GLSVNTLEQALKARHLDAVAYFGVGPIFPTPSKKDKQVVGVELLKKIRDSGVKKPLIAIG
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEC
GITTHNASKLREYGGIAVISAITQARDKALAIEKLLNNA
CCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFDANCLKLMFVAGSQDFYHIKGGKNDRINALLDTLELALQSQITAFQFRQKGDLALQDP
CCCCCEEEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
VEIKQLALKCQKLCQKYGTPFIINDEVRLALELKADGVHVGQEDMAIEEVIALCKKHQFI
HHHHHHHHHHHHHHHHCCCCEEECCCEEEEEEEECCCEEECHHHHHHHHHHHHHHCCCEE
GLSVNTLEQALKARHLDAVAYFGVGPIFPTPSKKDKQVVGVELLKKIRDSGVKKPLIAIG
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEC
GITTHNASKLREYGGIAVISAITQARDKALAIEKLLNNA
CCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA