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The map label for this gene is cofH [H]

Identifier: 108563066

GI number: 108563066

Start: 667988

End: 669139

Strand: Direct

Name: cofH [H]

Synonym: HPAG1_0641

Alternate gene names: 108563066

Gene position: 667988-669139 (Clockwise)

Preceding gene: 108563065

Following gene: 108563067

Centisome position: 41.84

GC content: 40.89

Gene sequence:

>1152_bases
ATGGCAAGAAATGCAAAGGGCTATGCTTCAACCCTAACATGGACGATTACACGCAACACTTTGAATTTTCTATGGGAACA
AGGTTTTAAAATGTGCATTAACAGAGAAGAAATTTTGGATTTAATGAAAAACGCGCCCTTGAAAGAATTAGGGCAAAAGG
CTTTGAGAGTGAAGCAACGCTTGCACCCTGAAAACTTGACGACTTTTATTGTGGATAGGAATATCAATTACACCAATATT
TGTTTTGTGGATTGCAAGTTTTGCGCGTTCAAACGCACCTTAAAAGAAAAAGACGCCTATGTGTTGAGCTATGAAGAAAT
TGATCAAAAGATTGAAGAATTGCTCGCTATTGGCGGCACGCAGATCCTTTTTCAAGGGGGGGTGCACCCGCAGCTAAAGA
TTGATTATTATGAAAATTTAGTCAGCCATATCGCTCAAAAATTCCCCACCATCACCATTCATGGTTTTAGCGCGGTTGAA
ATTGATTACATTTCTAAAATCTCTAAATTGCCTTTAAAAGAAGTTTTAGAAAGGTTGAAAAACGCCGGTTTAAGCTCCAT
TCCAGGAGCGGGAGCAGAGATATTAAGCGATAGGGTGCGCGATGTGATCGCTCCTAAAAAATTGAGCAGTGATCGCTGGA
TTGAAGTGCATAGAATGGCACATCTTTGCGGGATTAAAAGCACGGCTACCATGATGTTTGGGAGCGTGGATAATGAAGAA
GATGTAATAGAGCATTTACAAAGGGTGCGCGATTTGCAAGATGAAACCGGCGGCTTTAGGGCTTTTATTTTATGGAGTTT
TCAGCCCGACAACACCCCCTTAAAAGAAGAAATCCCAAGCATTAAAAAAGCGAGTTCCAATCGGTATTTACGCTATTTGG
CATGCAGTAGGATTTTTTTAGATAACATTCAAAACATACAAAGCTCATGGGTTACTCAAGGCTCTATGATAGGGCAGTTA
GCCTTATTGTTTGGAGCGAATGATTTAGGGAGTGTGATGATGGAAGAAAATGTAGTGAAAGCGGCCGGAACGAGTTTTTG
CATGAATGAAGCGGGAATGATAGAGCTTATTGAAGACATTGGGAGCGTGGCGGCTAAACGAAACACCGCCTATGAAATCT
TAAAGCGTTATCCGGCAAAAGCAAAGGTATAA

Upstream 100 bases:

>100_bases
TGGAGAGCTTCTACAGGCTTGCAGATTGAATGGATTTCGCCCATGGGGCCTTTGGTGTTGATTTTCCCTATAGCGTTTTT
CAACCAATGGGGCGATGGCA

Downstream 100 bases:

>100_bases
ACAGCATGAAAAAAATTTTAATCATTTTATTATTAGGAGTTTTTATGGGGTTACAAGCGAGCGCTTTGACACACCAAGAA
ATCAATCAAGCTAAAGTCCC

Product: hypothetical protein

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 [H]

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MARNAKGYASTLTWTITRNTLNFLWEQGFKMCINREEILDLMKNAPLKELGQKALRVKQRLHPENLTTFIVDRNINYTNI
CFVDCKFCAFKRTLKEKDAYVLSYEEIDQKIEELLAIGGTQILFQGGVHPQLKIDYYENLVSHIAQKFPTITIHGFSAVE
IDYISKISKLPLKEVLERLKNAGLSSIPGAGAEILSDRVRDVIAPKKLSSDRWIEVHRMAHLCGIKSTATMMFGSVDNEE
DVIEHLQRVRDLQDETGGFRAFILWSFQPDNTPLKEEIPSIKKASSNRYLRYLACSRIFLDNIQNIQSSWVTQGSMIGQL
ALLFGANDLGSVMMEENVVKAAGTSFCMNEAGMIELIEDIGSVAAKRNTAYEILKRYPAKAKV

Sequences:

>Translated_383_residues
MARNAKGYASTLTWTITRNTLNFLWEQGFKMCINREEILDLMKNAPLKELGQKALRVKQRLHPENLTTFIVDRNINYTNI
CFVDCKFCAFKRTLKEKDAYVLSYEEIDQKIEELLAIGGTQILFQGGVHPQLKIDYYENLVSHIAQKFPTITIHGFSAVE
IDYISKISKLPLKEVLERLKNAGLSSIPGAGAEILSDRVRDVIAPKKLSSDRWIEVHRMAHLCGIKSTATMMFGSVDNEE
DVIEHLQRVRDLQDETGGFRAFILWSFQPDNTPLKEEIPSIKKASSNRYLRYLACSRIFLDNIQNIQSSWVTQGSMIGQL
ALLFGANDLGSVMMEENVVKAAGTSFCMNEAGMIELIEDIGSVAAKRNTAYEILKRYPAKAKV
>Mature_382_residues
ARNAKGYASTLTWTITRNTLNFLWEQGFKMCINREEILDLMKNAPLKELGQKALRVKQRLHPENLTTFIVDRNINYTNIC
FVDCKFCAFKRTLKEKDAYVLSYEEIDQKIEELLAIGGTQILFQGGVHPQLKIDYYENLVSHIAQKFPTITIHGFSAVEI
DYISKISKLPLKEVLERLKNAGLSSIPGAGAEILSDRVRDVIAPKKLSSDRWIEVHRMAHLCGIKSTATMMFGSVDNEED
VIEHLQRVRDLQDETGGFRAFILWSFQPDNTPLKEEIPSIKKASSNRYLRYLACSRIFLDNIQNIQSSWVTQGSMIGQLA
LLFGANDLGSVMMEENVVKAAGTSFCMNEAGMIELIEDIGSVAAKRNTAYEILKRYPAKAKV

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 43354; Mature: 43222

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARNAKGYASTLTWTITRNTLNFLWEQGFKMCINREEILDLMKNAPLKELGQKALRVKQR
CCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHH
LHPENLTTFIVDRNINYTNICFVDCKFCAFKRTLKEKDAYVLSYEEIDQKIEELLAIGGT
CCCCCCEEEEEECCCCEEEEEEEEHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCC
QILFQGGVHPQLKIDYYENLVSHIAQKFPTITIHGFSAVEIDYISKISKLPLKEVLERLK
EEEEECCCCCCEEHHHHHHHHHHHHHHCCEEEEECCCEEHHHHHHHHHCCCHHHHHHHHH
NAGLSSIPGAGAEILSDRVRDVIAPKKLSSDRWIEVHRMAHLCGIKSTATMMFGSVDNEE
HCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCHH
DVIEHLQRVRDLQDETGGFRAFILWSFQPDNTPLKEEIPSIKKASSNRYLRYLACSRIFL
HHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHCCHHHHCCCCCHHHHHHHHHHHH
DNIQNIQSSWVTQGSMIGQLALLFGANDLGSVMMEENVVKAAGTSFCMNEAGMIELIEDI
HHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHH
GSVAAKRNTAYEILKRYPAKAKV
HHHHHCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ARNAKGYASTLTWTITRNTLNFLWEQGFKMCINREEILDLMKNAPLKELGQKALRVKQR
CCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHH
LHPENLTTFIVDRNINYTNICFVDCKFCAFKRTLKEKDAYVLSYEEIDQKIEELLAIGGT
CCCCCCEEEEEECCCCEEEEEEEEHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCC
QILFQGGVHPQLKIDYYENLVSHIAQKFPTITIHGFSAVEIDYISKISKLPLKEVLERLK
EEEEECCCCCCEEHHHHHHHHHHHHHHCCEEEEECCCEEHHHHHHHHHCCCHHHHHHHHH
NAGLSSIPGAGAEILSDRVRDVIAPKKLSSDRWIEVHRMAHLCGIKSTATMMFGSVDNEE
HCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCHH
DVIEHLQRVRDLQDETGGFRAFILWSFQPDNTPLKEEIPSIKKASSNRYLRYLACSRIFL
HHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHCCHHHHCCCCCHHHHHHHHHHHH
DNIQNIQSSWVTQGSMIGQLALLFGANDLGSVMMEENVVKAAGTSFCMNEAGMIELIEDI
HHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHH
GSVAAKRNTAYEILKRYPAKAKV
HHHHHCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]