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The map label for this gene is slt [H]

Identifier: 108563055

GI number: 108563055

Start: 655058

End: 656740

Strand: Direct

Name: slt [H]

Synonym: HPAG1_0630

Alternate gene names: 108563055

Gene position: 655058-656740 (Clockwise)

Preceding gene: 108563054

Following gene: 108563056

Centisome position: 41.03

GC content: 38.15

Gene sequence:

>1683_bases
ATGCGTTTTTTTATTTTATTTTTTATGGGTGCACTTGGTGTTGGTTTTTCTCAAACTGAGTTCAATTTAAAAGATTTAGA
AAAAAAGCCCGCTGGGATCGTTAGGGATTATTATTTGTGGCGTTATATTAGCGATAAAAAAACCAGTTTAGAAAACGCTA
AAAAAGCCTATGAATTGACTCAAAATAAAAACAACGCCCTACAAAAGGCCATGCAAGAAAAAGGCTCAGACAATTCAGAA
AAAAACCCTGATGTTAAATTGCCTGAAGATATTTATTGCAAGCAAACGGCTTTAGAAAGCATGCTAGAAGAAACAGGCAC
TTTCCAAGCAAGCTGTATCGCTATCGCTTTAAAATCAAAAATCAGAGATTTTGATAAAATCCCCCTTCAAACCCTTAAGC
CCTTACAAATTAAAATCAAAGAGGCTTACCCCGTTCTCTATGAAGAATTAGAAATTTTGCAAAGTAAGCATGTGAGCGCT
TCTTTGTTTAAGGCTAACGCGCAAGTGTTTAGCGCGCTTTTCAACCATTTGAGTTATGAAAAAAAGCTCCAAATTTTTGA
AAAGCATATCCCTATTAAAGAGTTAAACCGCCTTTTAGACGAAGATTACCCGGCGTTTAACCGCTTGATCTATCAAGTTA
TTTTAGATCCTAAATTGGATCATTTTAAAGACGCTCTGGCTAAAAGTAACGCTACCCACAGCAACGCGCAAACCTTTTTC
ATCCTGGGGATTAATGAAATTTTGCGCAAAAAACCCTCTAAAGCGCTCAAGTATTTTGAACGCTCAGAAGCGGTGGTTAA
AGACGATGATTTTTCAAAAGACAGAGCGATTTTTTGGCAGTATTTAGTCTCTAAAAAGAAAAAAACTTTGGAGCGCCTTT
CACAAAGCCCAGCTTTAAACCTCTATAGTCTTTATGCGAGCCGAAAACTCCAAACCACGCCCAGTTACCGCATCATTTCT
CACATCCAGAATTTAAGCCAAGAAGATCCTCCTTTTAACACCTACGATCCTTTTTCGTGGCAAATTTTTAAGGAAAAAAC
CTTGAGTTTGAAAGATGAGGGCGCGTTTAATGCGATGCTAAAAAGCCTGTATTATGAAAAAAGCTCCCCTGAATTGACCT
ATCTTTTAAGCCAACGCAATAAAGACAAGATTTATTATTATTTATCCCCTTATGAAGGCATTATTGAATGGCAAAATGTT
GATGAAAAGGCTATGGCGTATGCGATCGCTAGGCAAGAAAGCTTTTTGCTCCCGGCAGTCATTTCGCGCTCGTTCGCTCT
AGGGCTTATGCAAATCATGCCCTTTAATGTAGGGCCTTTCGCTAAAAGCCTTGGCATGGATAATGTTGATCTAAACGACA
TGTTTAACCCCAACATCGCTCTCAAACTTGGCAATTATTACTTGAACCATTTGAAAAAAGAATTCAACCACCCCCTTTTT
GTCGCCTACGCCTATAACGCTGGGCCTGGGTTTTTAAGGAGGTGGTTAGAAAGCTCCAAACGATTTAAAGAAAAAAATCA
TTTTGAGCCATGGCTTAGCATGGAGCTTATGCCTTATAGCGAGACTCGCATGTATGGCTTTAGAGTCATGCTCAATTACT
TGATTTATCAAGAAATTTTTGGGAATTTCATCCCTATTGATGCATTTTTAGAACAAACTCTTAACTCAAAGGACAAACCA
TGA

Upstream 100 bases:

>100_bases
TAGATTTCGCTCCTTTAGTGGTAGTCATTGTGTTGAAATTTTTAGATTTAACCCTCATCCAATGGCTTTTCATGCTCGCT
AAAAACCTTTAAAGAAAATC

Downstream 100 bases:

>100_bases
TTAAAAAATGCCTTTTTCCTGCCGCTGGCTACGGCACGCGCTTTTTGCCGATCACTAAAACCATTCCTAAAGAAATGCTG
CCCATTGTGGATAAGCCTTT

Product: soluble lytic murein transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 560; Mature: 560

Protein sequence:

>560_residues
MRFFILFFMGALGVGFSQTEFNLKDLEKKPAGIVRDYYLWRYISDKKTSLENAKKAYELTQNKNNALQKAMQEKGSDNSE
KNPDVKLPEDIYCKQTALESMLEETGTFQASCIAIALKSKIRDFDKIPLQTLKPLQIKIKEAYPVLYEELEILQSKHVSA
SLFKANAQVFSALFNHLSYEKKLQIFEKHIPIKELNRLLDEDYPAFNRLIYQVILDPKLDHFKDALAKSNATHSNAQTFF
ILGINEILRKKPSKALKYFERSEAVVKDDDFSKDRAIFWQYLVSKKKKTLERLSQSPALNLYSLYASRKLQTTPSYRIIS
HIQNLSQEDPPFNTYDPFSWQIFKEKTLSLKDEGAFNAMLKSLYYEKSSPELTYLLSQRNKDKIYYYLSPYEGIIEWQNV
DEKAMAYAIARQESFLLPAVISRSFALGLMQIMPFNVGPFAKSLGMDNVDLNDMFNPNIALKLGNYYLNHLKKEFNHPLF
VAYAYNAGPGFLRRWLESSKRFKEKNHFEPWLSMELMPYSETRMYGFRVMLNYLIYQEIFGNFIPIDAFLEQTLNSKDKP

Sequences:

>Translated_560_residues
MRFFILFFMGALGVGFSQTEFNLKDLEKKPAGIVRDYYLWRYISDKKTSLENAKKAYELTQNKNNALQKAMQEKGSDNSE
KNPDVKLPEDIYCKQTALESMLEETGTFQASCIAIALKSKIRDFDKIPLQTLKPLQIKIKEAYPVLYEELEILQSKHVSA
SLFKANAQVFSALFNHLSYEKKLQIFEKHIPIKELNRLLDEDYPAFNRLIYQVILDPKLDHFKDALAKSNATHSNAQTFF
ILGINEILRKKPSKALKYFERSEAVVKDDDFSKDRAIFWQYLVSKKKKTLERLSQSPALNLYSLYASRKLQTTPSYRIIS
HIQNLSQEDPPFNTYDPFSWQIFKEKTLSLKDEGAFNAMLKSLYYEKSSPELTYLLSQRNKDKIYYYLSPYEGIIEWQNV
DEKAMAYAIARQESFLLPAVISRSFALGLMQIMPFNVGPFAKSLGMDNVDLNDMFNPNIALKLGNYYLNHLKKEFNHPLF
VAYAYNAGPGFLRRWLESSKRFKEKNHFEPWLSMELMPYSETRMYGFRVMLNYLIYQEIFGNFIPIDAFLEQTLNSKDKP
>Mature_560_residues
MRFFILFFMGALGVGFSQTEFNLKDLEKKPAGIVRDYYLWRYISDKKTSLENAKKAYELTQNKNNALQKAMQEKGSDNSE
KNPDVKLPEDIYCKQTALESMLEETGTFQASCIAIALKSKIRDFDKIPLQTLKPLQIKIKEAYPVLYEELEILQSKHVSA
SLFKANAQVFSALFNHLSYEKKLQIFEKHIPIKELNRLLDEDYPAFNRLIYQVILDPKLDHFKDALAKSNATHSNAQTFF
ILGINEILRKKPSKALKYFERSEAVVKDDDFSKDRAIFWQYLVSKKKKTLERLSQSPALNLYSLYASRKLQTTPSYRIIS
HIQNLSQEDPPFNTYDPFSWQIFKEKTLSLKDEGAFNAMLKSLYYEKSSPELTYLLSQRNKDKIYYYLSPYEGIIEWQNV
DEKAMAYAIARQESFLLPAVISRSFALGLMQIMPFNVGPFAKSLGMDNVDLNDMFNPNIALKLGNYYLNHLKKEFNHPLF
VAYAYNAGPGFLRRWLESSKRFKEKNHFEPWLSMELMPYSETRMYGFRVMLNYLIYQEIFGNFIPIDAFLEQTLNSKDKP

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=139, Percent_Identity=35.2517985611511, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 65362; Mature: 65362

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFFILFFMGALGVGFSQTEFNLKDLEKKPAGIVRDYYLWRYISDKKTSLENAKKAYELT
CHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
QNKNNALQKAMQEKGSDNSEKNPDVKLPEDIYCKQTALESMLEETGTFQASCIAIALKSK
CCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
IRDFDKIPLQTLKPLQIKIKEAYPVLYEELEILQSKHVSASLFKANAQVFSALFNHLSYE
HHHHHCCCHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHH
KKLQIFEKHIPIKELNRLLDEDYPAFNRLIYQVILDPKLDHFKDALAKSNATHSNAQTFF
HHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEEE
ILGINEILRKKPSKALKYFERSEAVVKDDDFSKDRAIFWQYLVSKKKKTLERLSQSPALN
EEEHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCH
LYSLYASRKLQTTPSYRIISHIQNLSQEDPPFNTYDPFSWQIFKEKTLSLKDEGAFNAML
HHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHCCCCCCHHHHHHH
KSLYYEKSSPELTYLLSQRNKDKIYYYLSPYEGIIEWQNVDEKAMAYAIARQESFLLPAV
HHHHHCCCCCCEEEHCCCCCCCEEEEEECCCCCCEECCCCCHHHHHHHHHHCCCHHHHHH
ISRSFALGLMQIMPFNVGPFAKSLGMDNVDLNDMFNPNIALKLGNYYLNHLKKEFNHPLF
HHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCEE
VAYAYNAGPGFLRRWLESSKRFKEKNHFEPWLSMELMPYSETRMYGFRVMLNYLIYQEIF
EEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
GNFIPIDAFLEQTLNSKDKP
CCCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRFFILFFMGALGVGFSQTEFNLKDLEKKPAGIVRDYYLWRYISDKKTSLENAKKAYELT
CHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
QNKNNALQKAMQEKGSDNSEKNPDVKLPEDIYCKQTALESMLEETGTFQASCIAIALKSK
CCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
IRDFDKIPLQTLKPLQIKIKEAYPVLYEELEILQSKHVSASLFKANAQVFSALFNHLSYE
HHHHHCCCHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHH
KKLQIFEKHIPIKELNRLLDEDYPAFNRLIYQVILDPKLDHFKDALAKSNATHSNAQTFF
HHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEEE
ILGINEILRKKPSKALKYFERSEAVVKDDDFSKDRAIFWQYLVSKKKKTLERLSQSPALN
EEEHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCH
LYSLYASRKLQTTPSYRIISHIQNLSQEDPPFNTYDPFSWQIFKEKTLSLKDEGAFNAML
HHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHCCCCCCHHHHHHH
KSLYYEKSSPELTYLLSQRNKDKIYYYLSPYEGIIEWQNVDEKAMAYAIARQESFLLPAV
HHHHHCCCCCCEEEHCCCCCCCEEEEEECCCCCCEECCCCCHHHHHHHHHHCCCHHHHHH
ISRSFALGLMQIMPFNVGPFAKSLGMDNVDLNDMFNPNIALKLGNYYLNHLKKEFNHPLF
HHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCEE
VAYAYNAGPGFLRRWLESSKRFKEKNHFEPWLSMELMPYSETRMYGFRVMLNYLIYQEIF
EEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
GNFIPIDAFLEQTLNSKDKP
CCCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]