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The map label for this gene is gidB [H]

Identifier: 108562809

GI number: 108562809

Start: 394832

End: 395368

Strand: Reverse

Name: gidB [H]

Synonym: HPAG1_0384

Alternate gene names: 108562809

Gene position: 395368-394832 (Counterclockwise)

Preceding gene: 108562810

Following gene: 108562808

Centisome position: 24.77

GC content: 35.2

Gene sequence:

>537_bases
ATGAACCCCTTATTGCAAGATTATGCACGCATCCTTTTAGAATGGAATCAAACGCACAACTTGAGCGGCGCGAAATGTTT
AAGCGAGTTAGAACCCCAGATCACAGACGCTCTAAAACCTTTAGAATTTATCAAAGATTTTAAAAGTTGTTTGGATATTG
GGAGCGGGGCGGGACTTCCTGCTATCCCTTTAGCCCTTGAAAAACCTGAAGTAAAATTCATTCTTTTAGAGCCAAGAATA
AAAAGAGCGGCTTTTTTAAACTACCTTAAAAGCGTTTTGCCTTTAAAAAATATTGAAATCATTAAAAAGCGTTTAGAAAA
TTATCAAAGCCCCTTACAAGTAGATTTAATCACTTCTAGAGCGGTCGCTAGCTCTTCTTTTTTGATAGAAAAAAGCCAAC
GCTTTCTAAAAGATAAGGGGTATTTTTTATTCTATAAAGGCGAGCAGTTAAAAGATGAAATCGCTTGTAAAGACACTGAA
TGCTTTATGCATCAAAAGCGTGTTTATTTTTACAAATCAAAGGAAAGTTTATGTTAA

Upstream 100 bases:

>100_bases
TAAGCGCGATGATAGGCTTAGAAAAAACCAAAGAAATCTACAAAATAGCCATAGAAAAGAAGTATCGTTTTTATTCTTAT
GGCGATGGGATGCTGATTTT

Downstream 100 bases:

>100_bases
GAATTTTAATCCCCTTACTCATTATCGTGTGGGTTTTATGGCGTTTGTTTTTGAGGCAAAAACCCCACAAAGACAACCAC
TCTTACACGCAACAAACCCC

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase [H]

Number of amino acids: Translated: 178; Mature: 178

Protein sequence:

>178_residues
MNPLLQDYARILLEWNQTHNLSGAKCLSELEPQITDALKPLEFIKDFKSCLDIGSGAGLPAIPLALEKPEVKFILLEPRI
KRAAFLNYLKSVLPLKNIEIIKKRLENYQSPLQVDLITSRAVASSSFLIEKSQRFLKDKGYFLFYKGEQLKDEIACKDTE
CFMHQKRVYFYKSKESLC

Sequences:

>Translated_178_residues
MNPLLQDYARILLEWNQTHNLSGAKCLSELEPQITDALKPLEFIKDFKSCLDIGSGAGLPAIPLALEKPEVKFILLEPRI
KRAAFLNYLKSVLPLKNIEIIKKRLENYQSPLQVDLITSRAVASSSFLIEKSQRFLKDKGYFLFYKGEQLKDEIACKDTE
CFMHQKRVYFYKSKESLC
>Mature_178_residues
MNPLLQDYARILLEWNQTHNLSGAKCLSELEPQITDALKPLEFIKDFKSCLDIGSGAGLPAIPLALEKPEVKFILLEPRI
KRAAFLNYLKSVLPLKNIEIIKKRLENYQSPLQVDLITSRAVASSSFLIEKSQRFLKDKGYFLFYKGEQLKDEIACKDTE
CFMHQKRVYFYKSKESLC

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA [H]

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family [H]

Homologues:

Organism=Escherichia coli, GI1790179, Length=149, Percent_Identity=33.5570469798658, Blast_Score=88, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003682 [H]

Pfam domain/function: PF02527 GidB [H]

EC number: =2.1.1.170 [H]

Molecular weight: Translated: 20522; Mature: 20522

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPLLQDYARILLEWNQTHNLSGAKCLSELEPQITDALKPLEFIKDFKSCLDIGSGAGLP
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
AIPLALEKPEVKFILLEPRIKRAAFLNYLKSVLPLKNIEIIKKRLENYQSPLQVDLITSR
CCCEEECCCCEEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEHHHH
AVASSSFLIEKSQRFLKDKGYFLFYKGEQLKDEIACKDTECFMHQKRVYFYKSKESLC
HHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHCCCHHHHHHHHHHEEEECCCCCC
>Mature Secondary Structure
MNPLLQDYARILLEWNQTHNLSGAKCLSELEPQITDALKPLEFIKDFKSCLDIGSGAGLP
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
AIPLALEKPEVKFILLEPRIKRAAFLNYLKSVLPLKNIEIIKKRLENYQSPLQVDLITSR
CCCEEECCCCEEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEHHHH
AVASSSFLIEKSQRFLKDKGYFLFYKGEQLKDEIACKDTECFMHQKRVYFYKSKESLC
HHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHCCCHHHHHHHHHHEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]