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The map label for this gene is queA

Identifier: 108562810

GI number: 108562810

Start: 395365

End: 396402

Strand: Reverse

Name: queA

Synonym: HPAG1_0385

Alternate gene names: 108562810

Gene position: 396402-395365 (Counterclockwise)

Preceding gene: 108562811

Following gene: 108562809

Centisome position: 24.83

GC content: 38.44

Gene sequence:

>1038_bases
TTGAAAGAATTTGATTTAGAAAGCTATGATTATTATTTGCCTAAGGAATTGATCGCCAACTACCCCGTTTTGCCCAAAGA
AAAGGCTAAATTGCTCGTCTATGAAAGGCGTTCGCAAAAAATCACGCACACCACTTTTGAGCATGTTTTAGATTTTTTCC
CTAAAAACGCCCTTGTGGTGTTGAACGACACTAAAGTGATGAAGGCCAGGCTTTTTGGATCTAAGCATGCCCTTTTGCCA
TCAAAAACGACCGAAGTGTTTTTCCACCGCTTTTTTAAAGACAATACCGCCCTAACTCAAATCAAGGGTAAGATCAAAGC
GGGGGATAAAATCTTTTTTGATGCAAATTATCACGCTGAAGTTTTGGAATTGCTTCATAACGGCCAACGCTTGATCGCTT
TTTATAACCATAAAACCCCCTTAAATCAAGAAAATATCTTAAAGCTTTTAGAGCAATACGGGCATATGCCCTTACCTCCT
TATATTAAAAGAACCGATGAAAGTTTGGATGCGCATGAATACCAGAGCGTGTTCGCTAAACACATCGGCGCGGTGGCTGC
CCCTACGGCGTCATTGCATTTTTCTCAAAATACCTTAGAAAAATTATTGAAAGATTTCAAGCACGCTTTTTTAACCTTGC
ATGTGGGGGCTGGGACTTTTCTTGGCGTAGAAACTAAAGATATTAGAGAGCATCAAATCCATACAGAGATTTTACACATT
CCTAAAAAAAGTCAAGAAATTTTGCGAGAATCCCAAGAGGTTTTATGCATCGGCACGACCGCTTTAAGGAGCGTGGAATA
TTTCAAACGCTTAAAAAACTCTAATCAAGAATCGTTTGAATGCGATATATTCTTGCATCTTGCTAATCCTATCCAGCATG
TCAATTATTTGCTCACTAATTTCCATTTGCCCAAATCAAGCCTTTTAATGCTTGTAAGCGCGATGATAGGCTTAGAAAAA
ACCAAAGAAATCTACAAAATAGCCATAGAAAAGAAGTATCGTTTTTATTCTTATGGCGATGGGATGCTGATTTTATGA

Upstream 100 bases:

>100_bases
GGCGTTGCCTTTAGTGGGGCTTTATGGGCTTTCTATTTTGATTGCCAAAATGGTCAATCCGGCTCCTAAAGATAACGAAA
ATGACACCAAAGAGAATTAG

Downstream 100 bases:

>100_bases
ACCCCTTATTGCAAGATTATGCACGCATCCTTTTAGAATGGAATCAAACGCACAACTTGAGCGGCGCGAAATGTTTAAGC
GAGTTAGAACCCCAGATCAC

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MKEFDLESYDYYLPKELIANYPVLPKEKAKLLVYERRSQKITHTTFEHVLDFFPKNALVVLNDTKVMKARLFGSKHALLP
SKTTEVFFHRFFKDNTALTQIKGKIKAGDKIFFDANYHAEVLELLHNGQRLIAFYNHKTPLNQENILKLLEQYGHMPLPP
YIKRTDESLDAHEYQSVFAKHIGAVAAPTASLHFSQNTLEKLLKDFKHAFLTLHVGAGTFLGVETKDIREHQIHTEILHI
PKKSQEILRESQEVLCIGTTALRSVEYFKRLKNSNQESFECDIFLHLANPIQHVNYLLTNFHLPKSSLLMLVSAMIGLEK
TKEIYKIAIEKKYRFYSYGDGMLIL

Sequences:

>Translated_345_residues
MKEFDLESYDYYLPKELIANYPVLPKEKAKLLVYERRSQKITHTTFEHVLDFFPKNALVVLNDTKVMKARLFGSKHALLP
SKTTEVFFHRFFKDNTALTQIKGKIKAGDKIFFDANYHAEVLELLHNGQRLIAFYNHKTPLNQENILKLLEQYGHMPLPP
YIKRTDESLDAHEYQSVFAKHIGAVAAPTASLHFSQNTLEKLLKDFKHAFLTLHVGAGTFLGVETKDIREHQIHTEILHI
PKKSQEILRESQEVLCIGTTALRSVEYFKRLKNSNQESFECDIFLHLANPIQHVNYLLTNFHLPKSSLLMLVSAMIGLEK
TKEIYKIAIEKKYRFYSYGDGMLIL
>Mature_345_residues
MKEFDLESYDYYLPKELIANYPVLPKEKAKLLVYERRSQKITHTTFEHVLDFFPKNALVVLNDTKVMKARLFGSKHALLP
SKTTEVFFHRFFKDNTALTQIKGKIKAGDKIFFDANYHAEVLELLHNGQRLIAFYNHKTPLNQENILKLLEQYGHMPLPP
YIKRTDESLDAHEYQSVFAKHIGAVAAPTASLHFSQNTLEKLLKDFKHAFLTLHVGAGTFLGVETKDIREHQIHTEILHI
PKKSQEILRESQEVLCIGTTALRSVEYFKRLKNSNQESFECDIFLHLANPIQHVNYLLTNFHLPKSSLLMLVSAMIGLEK
TKEIYKIAIEKKYRFYSYGDGMLIL

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=346, Percent_Identity=37.8612716763006, Blast_Score=222, Evalue=2e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_HELPH (Q1CUC0)

Other databases:

- EMBL:   CP000241
- RefSeq:   YP_627126.1
- ProteinModelPortal:   Q1CUC0
- SMR:   Q1CUC0
- STRING:   Q1CUC0
- GeneID:   4098936
- GenomeReviews:   CP000241_GR
- KEGG:   hpa:HPAG1_0385
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   CYSHEIN
- ProtClustDB:   PRK00147
- BioCyc:   HPYL357544:HPAG1_0385-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39996; Mature: 39996

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEFDLESYDYYLPKELIANYPVLPKEKAKLLVYERRSQKITHTTFEHVLDFFPKNALVV
CCCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEE
LNDTKVMKARLFGSKHALLPSKTTEVFFHRFFKDNTALTQIKGKIKAGDKIFFDANYHAE
EECCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCEECCCCEEEEECCCHHH
VLELLHNGQRLIAFYNHKTPLNQENILKLLEQYGHMPLPPYIKRTDESLDAHEYQSVFAK
HHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHH
HIGAVAAPTASLHFSQNTLEKLLKDFKHAFLTLHVGAGTFLGVETKDIREHQIHTEILHI
HHCHHCCCCHHEEECHHHHHHHHHHHHHHEEEEEECCCEEECCCCHHHHHHHHHHHHHHC
PKKSQEILRESQEVLCIGTTALRSVEYFKRLKNSNQESFECDIFLHLANPIQHVNYLLTN
CCHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCHHHHHHHHHHH
FHLPKSSLLMLVSAMIGLEKTKEIYKIAIEKKYRFYSYGDGMLIL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCEEEC
>Mature Secondary Structure
MKEFDLESYDYYLPKELIANYPVLPKEKAKLLVYERRSQKITHTTFEHVLDFFPKNALVV
CCCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEE
LNDTKVMKARLFGSKHALLPSKTTEVFFHRFFKDNTALTQIKGKIKAGDKIFFDANYHAE
EECCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCEECCCCEEEEECCCHHH
VLELLHNGQRLIAFYNHKTPLNQENILKLLEQYGHMPLPPYIKRTDESLDAHEYQSVFAK
HHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHH
HIGAVAAPTASLHFSQNTLEKLLKDFKHAFLTLHVGAGTFLGVETKDIREHQIHTEILHI
HHCHHCCCCHHEEECHHHHHHHHHHHHHHEEEEEECCCEEECCCCHHHHHHHHHHHHHHC
PKKSQEILRESQEVLCIGTTALRSVEYFKRLKNSNQESFECDIFLHLANPIQHVNYLLTN
CCHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCHHHHHHHHHHH
FHLPKSSLLMLVSAMIGLEKTKEIYKIAIEKKYRFYSYGDGMLIL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA