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The map label for this gene is proS [H]

Identifier: 108562666

GI number: 108562666

Start: 245046

End: 246779

Strand: Reverse

Name: proS [H]

Synonym: HPAG1_0241

Alternate gene names: 108562666

Gene position: 246779-245046 (Counterclockwise)

Preceding gene: 108562667

Following gene: 108562665

Centisome position: 15.46

GC content: 40.54

Gene sequence:

>1734_bases
ATGCTATTTTCAAAACTCTTTGCCCCCACTCTCAAAGAACCCCCTAAAGATGCCGTGTTAAAAAGCCATAAACACTTAGC
TCAAGCAGGATACATTTATCAAGTAGGCAGCGGGATTTATAATTTTTTGCCTTTAGCTAAAAAAGTGCTAGACAAAATAG
AAAACATCACGCACAAACGCATGCAAGAGCATGGGGCGCAAAATATTTTAATGAGCTTTGTGGTTTTGGCGAGTTTGTGG
GAAAAATCAGGCCGTTTGGATAAATACGGCAAGGAATTACTGGTTTTTAAAGACCGAAAAGACAATGATTTTGTTTTAAG
CCCCACTTTAGAAGAAAATATCACCGAAATTGCCGCTAATTTCATCAAAAGCTACAAGCAATTACCCGTACATCTCTATC
AAATCCACACGAAATTCCGTGATGAAATCCGCCCACGATTCGGGCTGGTGAGAGCGAGGGAATTTATCATGAAAGATGGT
TATAGCTTTCATGAAGACGCTGAGAGCTTGGATAAGGAATTTTTAAACACGCAAAGCGCTTATAAAGAGATTTTAAACGA
TTTGGGTTTGGATTTTCGCATTGTGGAAGCGGATAGCGGGGCGATTGGAGGGAGTAAAAGCAAGGAATTTGTCGTTTTAA
CAGAATGCGGGGAAGACACGATCGTGGTGTGTAAAAATTGCGATTATGCGGCCAACATTGAAATCGCTAAACGCTCTAAA
AGGCCTGAGCCTTTAAATGTCCCAAAAGCGCAATTAGCGAAATTCCCTACCCCTAATACCACCAGCGCGCAAAGCGTGGC
GGAGTTTTTTAAAACAGAGCCTTATTTTGTTTTAAAAGCGCTTGTTAGAAAAGTGATCCATAAAGATAAAGAAACCCTAG
CGTGCTTTTTTGTTAGGGGCGATGACAATTTAGAGGAAGTTAAGGCTCTAAACGCCTTGAACATTATAGGGGCGAACGCT
TTAGAATTGAGAGAGGCGAGTCAAAAAGATTTGGATAGTGCGGGGTTAATAGCGGGTTTTATAGGGCCTTATGGCTTGAA
AAAGCATGTTCCTTACATTATTTTTGATGAAGATTTAAAAGAGGGCGATTGCTTGATCACTGGGGCTAATGAAAAGGATT
TTCATGCGGTGGGCGTGGATTTAAAAGGGTTTGAAAATCTTGTTTATGCGGATATTGTCCAGGTTAAAGAAAGCGATCAT
TGCCCTAATTGTCAAGGGGCGTTGAAATACCATAAGAGTTTGGAAGTGGGGCATATTTTCAAACTCGGGCAAGGCTATGC
TAAAAGCTTAAGGGCTAGTTTCTTGGATAAGAATGGTAAGGAGCAATTTTTTGAAATGGGGTGCTATGGGATAGGCATTA
GCCGATTGCTCAGCGCGATTTTAGAGCAAAAAAGCGATGATTTAGGCTGTGTGTGGACGAAAAATACCGCTCCTTTTGAT
GTGGTGATTGTGGTTTCTAATTGGAAAGATGAAGCGCAAAAAAAACTCGCTTTTGAAGTGTATGAAAGGCTGCTCCAAAA
GGGCGTTGATGCGCTGTTAGATGACAGAGATGCGCGTTTTGGGGCGAAGATGAGGGATTTTGAATTGATTGGGGAACGAT
TGGCGCTCATTATTGGGAAGCAAACTTTAGAGAATAAAGAATTTGAATGCATCAAACGCGCTAATTTAGAAAAACAAACG
ATCAAAGACATTGAATTAGAAGAAAAAATTTTAGAAATGTTAGCAAGCGAATAA

Upstream 100 bases:

>100_bases
TGTGGGCGCGATTAAAAACTTGTTTAATTTAGACAAATCTAACGCTAACCATGCCCAGAATTTGAATCTCTATAAATGCG
AATATTACGAGGAATAATGC

Downstream 100 bases:

>100_bases
GGGGGAGTGAGTGGGAAATTTAGTGATTGGCTCTAGGGGGAGCGAACTAGCCTTATGGCAAGCGAATCACATTAAAGAAC
GCCTGAAAAAAGAATGCTTG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MLFSKLFAPTLKEPPKDAVLKSHKHLAQAGYIYQVGSGIYNFLPLAKKVLDKIENITHKRMQEHGAQNILMSFVVLASLW
EKSGRLDKYGKELLVFKDRKDNDFVLSPTLEENITEIAANFIKSYKQLPVHLYQIHTKFRDEIRPRFGLVRAREFIMKDG
YSFHEDAESLDKEFLNTQSAYKEILNDLGLDFRIVEADSGAIGGSKSKEFVVLTECGEDTIVVCKNCDYAANIEIAKRSK
RPEPLNVPKAQLAKFPTPNTTSAQSVAEFFKTEPYFVLKALVRKVIHKDKETLACFFVRGDDNLEEVKALNALNIIGANA
LELREASQKDLDSAGLIAGFIGPYGLKKHVPYIIFDEDLKEGDCLITGANEKDFHAVGVDLKGFENLVYADIVQVKESDH
CPNCQGALKYHKSLEVGHIFKLGQGYAKSLRASFLDKNGKEQFFEMGCYGIGISRLLSAILEQKSDDLGCVWTKNTAPFD
VVIVVSNWKDEAQKKLAFEVYERLLQKGVDALLDDRDARFGAKMRDFELIGERLALIIGKQTLENKEFECIKRANLEKQT
IKDIELEEKILEMLASE

Sequences:

>Translated_577_residues
MLFSKLFAPTLKEPPKDAVLKSHKHLAQAGYIYQVGSGIYNFLPLAKKVLDKIENITHKRMQEHGAQNILMSFVVLASLW
EKSGRLDKYGKELLVFKDRKDNDFVLSPTLEENITEIAANFIKSYKQLPVHLYQIHTKFRDEIRPRFGLVRAREFIMKDG
YSFHEDAESLDKEFLNTQSAYKEILNDLGLDFRIVEADSGAIGGSKSKEFVVLTECGEDTIVVCKNCDYAANIEIAKRSK
RPEPLNVPKAQLAKFPTPNTTSAQSVAEFFKTEPYFVLKALVRKVIHKDKETLACFFVRGDDNLEEVKALNALNIIGANA
LELREASQKDLDSAGLIAGFIGPYGLKKHVPYIIFDEDLKEGDCLITGANEKDFHAVGVDLKGFENLVYADIVQVKESDH
CPNCQGALKYHKSLEVGHIFKLGQGYAKSLRASFLDKNGKEQFFEMGCYGIGISRLLSAILEQKSDDLGCVWTKNTAPFD
VVIVVSNWKDEAQKKLAFEVYERLLQKGVDALLDDRDARFGAKMRDFELIGERLALIIGKQTLENKEFECIKRANLEKQT
IKDIELEEKILEMLASE
>Mature_577_residues
MLFSKLFAPTLKEPPKDAVLKSHKHLAQAGYIYQVGSGIYNFLPLAKKVLDKIENITHKRMQEHGAQNILMSFVVLASLW
EKSGRLDKYGKELLVFKDRKDNDFVLSPTLEENITEIAANFIKSYKQLPVHLYQIHTKFRDEIRPRFGLVRAREFIMKDG
YSFHEDAESLDKEFLNTQSAYKEILNDLGLDFRIVEADSGAIGGSKSKEFVVLTECGEDTIVVCKNCDYAANIEIAKRSK
RPEPLNVPKAQLAKFPTPNTTSAQSVAEFFKTEPYFVLKALVRKVIHKDKETLACFFVRGDDNLEEVKALNALNIIGANA
LELREASQKDLDSAGLIAGFIGPYGLKKHVPYIIFDEDLKEGDCLITGANEKDFHAVGVDLKGFENLVYADIVQVKESDH
CPNCQGALKYHKSLEVGHIFKLGQGYAKSLRASFLDKNGKEQFFEMGCYGIGISRLLSAILEQKSDDLGCVWTKNTAPFD
VVIVVSNWKDEAQKKLAFEVYERLLQKGVDALLDDRDARFGAKMRDFELIGERLALIIGKQTLENKEFECIKRANLEKQT
IKDIELEEKILEMLASE

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=243, Percent_Identity=40.3292181069959, Blast_Score=190, Evalue=3e-48,
Organism=Escherichia coli, GI1786392, Length=579, Percent_Identity=39.7236614853195, Blast_Score=406, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI115532348, Length=210, Percent_Identity=41.4285714285714, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI193203271, Length=85, Percent_Identity=37.6470588235294, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320931, Length=543, Percent_Identity=31.4917127071823, Blast_Score=244, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24656200, Length=242, Percent_Identity=37.603305785124, Blast_Score=176, Evalue=3e-44,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 65186; Mature: 65186

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFSKLFAPTLKEPPKDAVLKSHKHLAQAGYIYQVGSGIYNFLPLAKKVLDKIENITHKR
CCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
MQEHGAQNILMSFVVLASLWEKSGRLDKYGKELLVFKDRKDNDFVLSPTLEENITEIAAN
HHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEEECCCCCCEEECCCHHHHHHHHHHH
FIKSYKQLPVHLYQIHTKFRDEIRPRFGLVRAREFIMKDGYSFHEDAESLDKEFLNTQSA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
YKEILNDLGLDFRIVEADSGAIGGSKSKEFVVLTECGEDTIVVCKNCDYAANIEIAKRSK
HHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCHHHHCCC
RPEPLNVPKAQLAKFPTPNTTSAQSVAEFFKTEPYFVLKALVRKVIHKDKETLACFFVRG
CCCCCCCCHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEEEC
DDNLEEVKALNALNIIGANALELREASQKDLDSAGLIAGFIGPYGLKKHVPYIIFDEDLK
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCHHHCCCEEEECCCCC
EGDCLITGANEKDFHAVGVDLKGFENLVYADIVQVKESDHCPNCQGALKYHKSLEVGHIF
CCCEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHH
KLGQGYAKSLRASFLDKNGKEQFFEMGCYGIGISRLLSAILEQKSDDLGCVWTKNTAPFD
HHHHHHHHHHHHHHHCCCCHHHHHHHCHHHCCHHHHHHHHHHCCCCCCCEEEECCCCCEE
VVIVVSNWKDEAQKKLAFEVYERLLQKGVDALLDDRDARFGAKMRDFELIGERLALIIGK
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHH
QTLENKEFECIKRANLEKQTIKDIELEEKILEMLASE
HHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLFSKLFAPTLKEPPKDAVLKSHKHLAQAGYIYQVGSGIYNFLPLAKKVLDKIENITHKR
CCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
MQEHGAQNILMSFVVLASLWEKSGRLDKYGKELLVFKDRKDNDFVLSPTLEENITEIAAN
HHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEEECCCCCCEEECCCHHHHHHHHHHH
FIKSYKQLPVHLYQIHTKFRDEIRPRFGLVRAREFIMKDGYSFHEDAESLDKEFLNTQSA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
YKEILNDLGLDFRIVEADSGAIGGSKSKEFVVLTECGEDTIVVCKNCDYAANIEIAKRSK
HHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCHHHHCCC
RPEPLNVPKAQLAKFPTPNTTSAQSVAEFFKTEPYFVLKALVRKVIHKDKETLACFFVRG
CCCCCCCCHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEEEC
DDNLEEVKALNALNIIGANALELREASQKDLDSAGLIAGFIGPYGLKKHVPYIIFDEDLK
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCHHHCCCEEEECCCCC
EGDCLITGANEKDFHAVGVDLKGFENLVYADIVQVKESDHCPNCQGALKYHKSLEVGHIF
CCCEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHH
KLGQGYAKSLRASFLDKNGKEQFFEMGCYGIGISRLLSAILEQKSDDLGCVWTKNTAPFD
HHHHHHHHHHHHHHHCCCCHHHHHHHCHHHCCHHHHHHHHHHCCCCCCCEEEECCCCCEE
VVIVVSNWKDEAQKKLAFEVYERLLQKGVDALLDDRDARFGAKMRDFELIGERLALIIGK
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHH
QTLENKEFECIKRANLEKQTIKDIELEEKILEMLASE
HHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA