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The map label for this gene is metK [H]

Identifier: 108562616

GI number: 108562616

Start: 197831

End: 198988

Strand: Direct

Name: metK [H]

Synonym: HPAG1_0191

Alternate gene names: 108562616

Gene position: 197831-198988 (Clockwise)

Preceding gene: 108562615

Following gene: 108562617

Centisome position: 12.39

GC content: 44.99

Gene sequence:

>1158_bases
ATGAAAGATAGTTTTCTTTTCACTTCTGAATCAGTAACCGAGGGGCATCCTGACAAAATGGCTGATCAAATCAGCGATGC
GGTCTTGGATTACATTATTGAGCGCGATCAAAAAGCCAAAGTCGCATGCGAGACTTTAGTTTCTAATGGCTTTTGCATGA
TCACTGGCGAGTTAAAAACTTCTGTTTATGCCCCTATGCAAGAGATCGCAAGAGAAGTGGTTAAAAAAATTGGCTATACA
GACGCCCTTTATGGCTTTGATTACAGGAGTGCGGCGGTTTTAAATGGTATTGGCGAGCAAAGCCCTGATATTAATCAAGG
CGTGGATAGAGAAGATGGCGAGATTGGGGCAGGGGATCAAGGGCTTATGTTTGGTTATGCATGCAAAGAGACTGAAACGC
TCATGCCCTTACCCATCCATTTAGCGCACCAGCTCGCTTTCGCTCTGGCTCAAAAAAGAAAAGACAACACCTTGCCTTTT
TTAAGGCCTGATGGCAAGTCTCAAGTGAGCGTGCATTATGAAAACAACAAGCCTGTAAGCATTGATACGATCGTCATTTC
CACCCAACATTCCCCAGAAGTTTCGCAAAAGCATTTAAAAGAAGCCGTGATTGAAGAGATCGTGTATAAGGTTTTACCCA
AAGAATATTTGCATGACAATATCAAGTTTTTTGTCAATCCCACAGGAAAATTCGTTATCGGTGGGCCGCAAGGCGATGCG
GGTTTGACGGGCAGAAAAATCATCGTGGACACTTATGGGGGGAGTTGCCCGCATGGTGGGGGAGCGTTTAGCGGGAAAGA
CCCTAGCAAAGTGGATAGGAGCGCAGCTTATGCGGCCCGCTATGTGGCTAAAAATTTGGTAGCGAGTGGGGTTTGCGATA
AAGCGACCGTGCAGCTTGCTTATGCGATTGGGGTGATAGAGCCCGTGTCTATTTATGTGAACACGCATAACACGAGCAAG
TATTCAAGCGTGGAGTTGGAAAAATGCGTGAAAGCGGTTTTCAAACTCACGCCAAAAGGCATTATTGAAAGCCTGGATTT
GTTAAGACCCATTTATTCGCTCACTTCAGCTTATGGGCATTTTGGGCGCGAGTTAGAGGAATTCACTTGGGAAAAAACCA
ACAAAGATGAGGAGATTAAAGCGTTCTTTAAGCGTTAA

Upstream 100 bases:

>100_bases
TTGTTGCAAAAAGCTAAAGGGTTTTTTAAGTCTTAAAGAATGAAATAAGCTATAATAAGCCCATTTTGAATACGAATGAT
TATTTTAATAAGGACAATCA

Downstream 100 bases:

>100_bases
AAAATATTTTAAGGGTAATATTTTAAAAAATTTTTGTATAATCAACAATTCACAAGGAGTTTAAATTGAAACAAAGAACG
CTGTCTATTATTAAACCGGA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MKDSFLFTSESVTEGHPDKMADQISDAVLDYIIERDQKAKVACETLVSNGFCMITGELKTSVYAPMQEIAREVVKKIGYT
DALYGFDYRSAAVLNGIGEQSPDINQGVDREDGEIGAGDQGLMFGYACKETETLMPLPIHLAHQLAFALAQKRKDNTLPF
LRPDGKSQVSVHYENNKPVSIDTIVISTQHSPEVSQKHLKEAVIEEIVYKVLPKEYLHDNIKFFVNPTGKFVIGGPQGDA
GLTGRKIIVDTYGGSCPHGGGAFSGKDPSKVDRSAAYAARYVAKNLVASGVCDKATVQLAYAIGVIEPVSIYVNTHNTSK
YSSVELEKCVKAVFKLTPKGIIESLDLLRPIYSLTSAYGHFGRELEEFTWEKTNKDEEIKAFFKR

Sequences:

>Translated_385_residues
MKDSFLFTSESVTEGHPDKMADQISDAVLDYIIERDQKAKVACETLVSNGFCMITGELKTSVYAPMQEIAREVVKKIGYT
DALYGFDYRSAAVLNGIGEQSPDINQGVDREDGEIGAGDQGLMFGYACKETETLMPLPIHLAHQLAFALAQKRKDNTLPF
LRPDGKSQVSVHYENNKPVSIDTIVISTQHSPEVSQKHLKEAVIEEIVYKVLPKEYLHDNIKFFVNPTGKFVIGGPQGDA
GLTGRKIIVDTYGGSCPHGGGAFSGKDPSKVDRSAAYAARYVAKNLVASGVCDKATVQLAYAIGVIEPVSIYVNTHNTSK
YSSVELEKCVKAVFKLTPKGIIESLDLLRPIYSLTSAYGHFGRELEEFTWEKTNKDEEIKAFFKR
>Mature_385_residues
MKDSFLFTSESVTEGHPDKMADQISDAVLDYIIERDQKAKVACETLVSNGFCMITGELKTSVYAPMQEIAREVVKKIGYT
DALYGFDYRSAAVLNGIGEQSPDINQGVDREDGEIGAGDQGLMFGYACKETETLMPLPIHLAHQLAFALAQKRKDNTLPF
LRPDGKSQVSVHYENNKPVSIDTIVISTQHSPEVSQKHLKEAVIEEIVYKVLPKEYLHDNIKFFVNPTGKFVIGGPQGDA
GLTGRKIIVDTYGGSCPHGGGAFSGKDPSKVDRSAAYAARYVAKNLVASGVCDKATVQLAYAIGVIEPVSIYVNTHNTSK
YSSVELEKCVKAVFKLTPKGIIESLDLLRPIYSLTSAYGHFGRELEEFTWEKTNKDEEIKAFFKR

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=378, Percent_Identity=54.7619047619048, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI4557737, Length=377, Percent_Identity=52.5198938992042, Blast_Score=380, Evalue=1e-105,
Organism=Escherichia coli, GI1789311, Length=376, Percent_Identity=62.2340425531915, Blast_Score=478, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI17551082, Length=378, Percent_Identity=51.8518518518518, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25145633, Length=389, Percent_Identity=50.6426735218509, Blast_Score=377, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17538494, Length=374, Percent_Identity=52.4064171122995, Blast_Score=374, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17538498, Length=374, Percent_Identity=51.8716577540107, Blast_Score=371, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI32565905, Length=374, Percent_Identity=48.1283422459893, Blast_Score=321, Evalue=5e-88,
Organism=Caenorhabditis elegans, GI25145635, Length=338, Percent_Identity=48.2248520710059, Blast_Score=308, Evalue=3e-84,
Organism=Caenorhabditis elegans, GI32565911, Length=326, Percent_Identity=49.3865030674847, Blast_Score=305, Evalue=3e-83,
Organism=Caenorhabditis elegans, GI32565903, Length=301, Percent_Identity=50.8305647840532, Blast_Score=284, Evalue=7e-77,
Organism=Caenorhabditis elegans, GI32565909, Length=82, Percent_Identity=56.0975609756098, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320710, Length=380, Percent_Identity=54.2105263157895, Blast_Score=403, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6323209, Length=378, Percent_Identity=52.9100529100529, Blast_Score=392, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580527, Length=375, Percent_Identity=51.4666666666667, Blast_Score=355, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580525, Length=375, Percent_Identity=51.4666666666667, Blast_Score=355, Evalue=2e-98,
Organism=Drosophila melanogaster, GI45552159, Length=375, Percent_Identity=51.7333333333333, Blast_Score=355, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580529, Length=375, Percent_Identity=51.7333333333333, Blast_Score=355, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580537, Length=375, Percent_Identity=51.7333333333333, Blast_Score=355, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580533, Length=375, Percent_Identity=51.7333333333333, Blast_Score=355, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580535, Length=375, Percent_Identity=51.7333333333333, Blast_Score=355, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580531, Length=375, Percent_Identity=51.7333333333333, Blast_Score=355, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580541, Length=361, Percent_Identity=50.1385041551247, Blast_Score=331, Evalue=6e-91,
Organism=Drosophila melanogaster, GI24580539, Length=119, Percent_Identity=50.4201680672269, Blast_Score=126, Evalue=2e-29,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42394; Mature: 42394

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDSFLFTSESVTEGHPDKMADQISDAVLDYIIERDQKAKVACETLVSNGFCMITGELKT
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEECHHH
SVYAPMQEIAREVVKKIGYTDALYGFDYRSAAVLNGIGEQSPDINQGVDREDGEIGAGDQ
HHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
GLMFGYACKETETLMPLPIHLAHQLAFALAQKRKDNTLPFLRPDGKSQVSVHYENNKPVS
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCCCEEEEEECCCCCEE
IDTIVISTQHSPEVSQKHLKEAVIEEIVYKVLPKEYLHDNIKFFVNPTGKFVIGGPQGDA
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCCCEEEECCCCCC
GLTGRKIIVDTYGGSCPHGGGAFSGKDPSKVDRSAAYAARYVAKNLVASGVCDKATVQLA
CCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
YAIGVIEPVSIYVNTHNTSKYSSVELEKCVKAVFKLTPKGIIESLDLLRPIYSLTSAYGH
HHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
FGRELEEFTWEKTNKDEEIKAFFKR
HHHHHHHHHHCCCCCCHHHHHHHCC
>Mature Secondary Structure
MKDSFLFTSESVTEGHPDKMADQISDAVLDYIIERDQKAKVACETLVSNGFCMITGELKT
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEECHHH
SVYAPMQEIAREVVKKIGYTDALYGFDYRSAAVLNGIGEQSPDINQGVDREDGEIGAGDQ
HHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
GLMFGYACKETETLMPLPIHLAHQLAFALAQKRKDNTLPFLRPDGKSQVSVHYENNKPVS
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCCCEEEEEECCCCCEE
IDTIVISTQHSPEVSQKHLKEAVIEEIVYKVLPKEYLHDNIKFFVNPTGKFVIGGPQGDA
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCCCEEEECCCCCC
GLTGRKIIVDTYGGSCPHGGGAFSGKDPSKVDRSAAYAARYVAKNLVASGVCDKATVQLA
CCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
YAIGVIEPVSIYVNTHNTSKYSSVELEKCVKAVFKLTPKGIIESLDLLRPIYSLTSAYGH
HHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
FGRELEEFTWEKTNKDEEIKAFFKR
HHHHHHHHHHCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA