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The map label for this gene is eno [H]

Identifier: 108562577

GI number: 108562577

Start: 159636

End: 160916

Strand: Direct

Name: eno [H]

Synonym: HPAG1_0152

Alternate gene names: 108562577

Gene position: 159636-160916 (Clockwise)

Preceding gene: 108562576

Following gene: 108562578

Centisome position: 10.0

GC content: 43.72

Gene sequence:

>1281_bases
ATGCTAACCATTAAAGATATTCATGCTTTAGAAGTGATGGATAGTAGGGGCAATCCTACCATTCAAGCTAGCGTGATTTT
AAGCGATAACACTAAGGCGAGCGCGATTGTGCCTAGCGGGGCGAGCACCGGTAAAAGAGAAGCGTTAGAATTAAGGGATA
ATGACAAAACCCGTTTTTTGGGTAAAGGGGTTTTAAGGGCATGCGAGAATGTCAATAGCGTGATCAAACACCATTTAATA
GGGCTTGAAGCGATCAATCAAGCCTTTGTAGATGAGAGGTTAAGGGCTTTAGACGGCACGCCTAATTACGCTAATTTAGG
GGCGAACGCTGTTTTGGGCGTTTCTATGGCGTTAGCAAGGGCTAGCGCGAAGGCTTTAAATCTGCCATTATACCGCTATT
TAGGGGGGGCTAACGCTCTGACTTTGCCTGTGCCGATGCTCAATATCATCAACGGCGGAACGCATGCGAATAATTCCATA
GACTTCCAAGAATACATGATCATGCCCTTAGGGTTTGAAAGTTTTAGAGAAGCCTTAAGAGCGAGCACGGAAGTCTATCA
CACGCTTAAAAAACTTTTAGATGGGAAGAATCAGCTCACAAGCGTGGGCGATGAGGGGGGCTTTGCGCCTAATTTTAGCA
ACAATGTAGAACCCCTTGAAGCCATTTCTCAAGCCATTGAAAAAGCCGGCTATAAGTTAGGCGAAGAAATCGCACTCGCT
TTAGATGTAGCGAGCAGCGAATTGGTGGATGAAAATTTCAATTACCATTTAAAGGGTGAAAATAAGATTTTAGATTCGCA
TGAATTAGTGGCTTATTATAAAGAGTTGGTGGCAAAATACCCGATTGTATCCATTGAAGATGGTTTGAGCGAAGACGATT
GGGAGGGTTGGGCGTTTTTAAGCAAGGAATTAGGGCGTCAAATCCAGTTGGTGGGCGATGATTTGTTTGTAACGAACGCA
AGCATTTTGCAAAAAGGCATTGAAAAAAACATCGCGAACGCCATTTTAATCAAACCCAATCAAATCGGCACCATTAGTGA
GACTTTAGAGACCATAAGATTAGCCAAACACCATGCCTATCAATGCGTGATGAGCCATAGAAGCGGGGAGAGTGAGGACA
GCTTTATCGCTGATTTTGCCGTTGCATTGAATGCGGGAGAAATTAAAACCGGATCCACCGCAAGGAGTGAAAGGATCGCT
AAATACAACCGCCTTTTAGAGATTGAGCATGAATTAAAAGGGGGGATTTATATCGGTAAAGAGTTGTTTAAGCATGGCTA
G

Upstream 100 bases:

>100_bases
TGAAATCACTCTTAAGATTAAAGAGAGTATTGGCTCTAATGAAGAGATCATGCCCTTACCAGATGAGCCTTTAGAAGAAA
TGGAATAAAAAAGGATTTTG

Downstream 100 bases:

>100_bases
TGGCCTTTTTGAAAACGATGGAATCAAAGAAAACAAAGTGCGAGATTTTTTCTATAGCCATAGCTCCCTTATTGTCTTTT
TCCTCTTACTGCTTGGGTTT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MLTIKDIHALEVMDSRGNPTIQASVILSDNTKASAIVPSGASTGKREALELRDNDKTRFLGKGVLRACENVNSVIKHHLI
GLEAINQAFVDERLRALDGTPNYANLGANAVLGVSMALARASAKALNLPLYRYLGGANALTLPVPMLNIINGGTHANNSI
DFQEYMIMPLGFESFREALRASTEVYHTLKKLLDGKNQLTSVGDEGGFAPNFSNNVEPLEAISQAIEKAGYKLGEEIALA
LDVASSELVDENFNYHLKGENKILDSHELVAYYKELVAKYPIVSIEDGLSEDDWEGWAFLSKELGRQIQLVGDDLFVTNA
SILQKGIEKNIANAILIKPNQIGTISETLETIRLAKHHAYQCVMSHRSGESEDSFIADFAVALNAGEIKTGSTARSERIA
KYNRLLEIEHELKGGIYIGKELFKHG

Sequences:

>Translated_426_residues
MLTIKDIHALEVMDSRGNPTIQASVILSDNTKASAIVPSGASTGKREALELRDNDKTRFLGKGVLRACENVNSVIKHHLI
GLEAINQAFVDERLRALDGTPNYANLGANAVLGVSMALARASAKALNLPLYRYLGGANALTLPVPMLNIINGGTHANNSI
DFQEYMIMPLGFESFREALRASTEVYHTLKKLLDGKNQLTSVGDEGGFAPNFSNNVEPLEAISQAIEKAGYKLGEEIALA
LDVASSELVDENFNYHLKGENKILDSHELVAYYKELVAKYPIVSIEDGLSEDDWEGWAFLSKELGRQIQLVGDDLFVTNA
SILQKGIEKNIANAILIKPNQIGTISETLETIRLAKHHAYQCVMSHRSGESEDSFIADFAVALNAGEIKTGSTARSERIA
KYNRLLEIEHELKGGIYIGKELFKHG
>Mature_426_residues
MLTIKDIHALEVMDSRGNPTIQASVILSDNTKASAIVPSGASTGKREALELRDNDKTRFLGKGVLRACENVNSVIKHHLI
GLEAINQAFVDERLRALDGTPNYANLGANAVLGVSMALARASAKALNLPLYRYLGGANALTLPVPMLNIINGGTHANNSI
DFQEYMIMPLGFESFREALRASTEVYHTLKKLLDGKNQLTSVGDEGGFAPNFSNNVEPLEAISQAIEKAGYKLGEEIALA
LDVASSELVDENFNYHLKGENKILDSHELVAYYKELVAKYPIVSIEDGLSEDDWEGWAFLSKELGRQIQLVGDDLFVTNA
SILQKGIEKNIANAILIKPNQIGTISETLETIRLAKHHAYQCVMSHRSGESEDSFIADFAVALNAGEIKTGSTARSERIA
KYNRLLEIEHELKGGIYIGKELFKHG

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=422, Percent_Identity=47.6303317535545, Blast_Score=388, Evalue=1e-108,
Organism=Homo sapiens, GI4503571, Length=422, Percent_Identity=45.9715639810426, Blast_Score=370, Evalue=1e-102,
Organism=Homo sapiens, GI301897477, Length=435, Percent_Identity=45.5172413793103, Blast_Score=367, Evalue=1e-102,
Organism=Homo sapiens, GI301897469, Length=435, Percent_Identity=45.5172413793103, Blast_Score=367, Evalue=1e-102,
Organism=Homo sapiens, GI301897479, Length=433, Percent_Identity=42.0323325635104, Blast_Score=323, Evalue=1e-88,
Organism=Homo sapiens, GI169201331, Length=340, Percent_Identity=26.4705882352941, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI169201757, Length=340, Percent_Identity=26.4705882352941, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI239744207, Length=340, Percent_Identity=26.4705882352941, Blast_Score=112, Evalue=6e-25,
Organism=Escherichia coli, GI1789141, Length=417, Percent_Identity=53.7170263788969, Blast_Score=433, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI71995829, Length=431, Percent_Identity=46.8677494199536, Blast_Score=380, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17536383, Length=423, Percent_Identity=47.2813238770686, Blast_Score=379, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI32563855, Length=190, Percent_Identity=43.1578947368421, Blast_Score=164, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6321693, Length=439, Percent_Identity=45.7858769931663, Blast_Score=351, Evalue=1e-97,
Organism=Saccharomyces cerevisiae, GI6324974, Length=435, Percent_Identity=43.6781609195402, Blast_Score=338, Evalue=1e-93,
Organism=Saccharomyces cerevisiae, GI6324969, Length=435, Percent_Identity=43.6781609195402, Blast_Score=338, Evalue=1e-93,
Organism=Saccharomyces cerevisiae, GI6323985, Length=435, Percent_Identity=43.6781609195402, Blast_Score=337, Evalue=2e-93,
Organism=Saccharomyces cerevisiae, GI6321968, Length=439, Percent_Identity=45.7858769931663, Blast_Score=333, Evalue=4e-92,
Organism=Drosophila melanogaster, GI24580918, Length=435, Percent_Identity=45.9770114942529, Blast_Score=359, Evalue=2e-99,
Organism=Drosophila melanogaster, GI24580916, Length=435, Percent_Identity=45.9770114942529, Blast_Score=359, Evalue=2e-99,
Organism=Drosophila melanogaster, GI24580920, Length=435, Percent_Identity=45.9770114942529, Blast_Score=359, Evalue=2e-99,
Organism=Drosophila melanogaster, GI24580914, Length=435, Percent_Identity=45.9770114942529, Blast_Score=359, Evalue=2e-99,
Organism=Drosophila melanogaster, GI281360527, Length=435, Percent_Identity=45.9770114942529, Blast_Score=358, Evalue=3e-99,
Organism=Drosophila melanogaster, GI17137654, Length=435, Percent_Identity=45.9770114942529, Blast_Score=358, Evalue=3e-99,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 46562; Mature: 46562

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTIKDIHALEVMDSRGNPTIQASVILSDNTKASAIVPSGASTGKREALELRDNDKTRFL
CCEECCCHHHHHCCCCCCCEEEEEEEEECCCCCEEEECCCCCCCCHHHHHCCCCCCHHHH
GKGVLRACENVNSVIKHHLIGLEAINQAFVDERLRALDGTPNYANLGANAVLGVSMALAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHH
ASAKALNLPLYRYLGGANALTLPVPMLNIINGGTHANNSIDFQEYMIMPLGFESFREALR
HHHHHHCCCHHHHHCCCCEEEECCHHHHHHCCCCCCCCCCCHHHHEEECCCHHHHHHHHH
ASTEVYHTLKKLLDGKNQLTSVGDEGGFAPNFSNNVEPLEAISQAIEKAGYKLGEEIALA
HHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHEEE
LDVASSELVDENFNYHLKGENKILDSHELVAYYKELVAKYPIVSIEDGLSEDDWEGWAFL
EEHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHH
SKELGRQIQLVGDDLFVTNASILQKGIEKNIANAILIKPNQIGTISETLETIRLAKHHAY
HHHHCCEEEEECCCEEEECHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHH
QCVMSHRSGESEDSFIADFAVALNAGEIKTGSTARSERIAKYNRLLEIEHELKGGIYIGK
HHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECH
ELFKHG
HHHHCC
>Mature Secondary Structure
MLTIKDIHALEVMDSRGNPTIQASVILSDNTKASAIVPSGASTGKREALELRDNDKTRFL
CCEECCCHHHHHCCCCCCCEEEEEEEEECCCCCEEEECCCCCCCCHHHHHCCCCCCHHHH
GKGVLRACENVNSVIKHHLIGLEAINQAFVDERLRALDGTPNYANLGANAVLGVSMALAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHH
ASAKALNLPLYRYLGGANALTLPVPMLNIINGGTHANNSIDFQEYMIMPLGFESFREALR
HHHHHHCCCHHHHHCCCCEEEECCHHHHHHCCCCCCCCCCCHHHHEEECCCHHHHHHHHH
ASTEVYHTLKKLLDGKNQLTSVGDEGGFAPNFSNNVEPLEAISQAIEKAGYKLGEEIALA
HHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHEEE
LDVASSELVDENFNYHLKGENKILDSHELVAYYKELVAKYPIVSIEDGLSEDDWEGWAFL
EEHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHH
SKELGRQIQLVGDDLFVTNASILQKGIEKNIANAILIKPNQIGTISETLETIRLAKHHAY
HHHHCCEEEEECCCEEEECHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHH
QCVMSHRSGESEDSFIADFAVALNAGEIKTGSTARSERIAKYNRLLEIEHELKGGIYIGK
HHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECH
ELFKHG
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA