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The map label for this gene is topA [H]

Identifier: 108562541

GI number: 108562541

Start: 122006

End: 124216

Strand: Direct

Name: topA [H]

Synonym: HPAG1_0116

Alternate gene names: 108562541

Gene position: 122006-124216 (Clockwise)

Preceding gene: 108562538

Following gene: 108562542

Centisome position: 7.64

GC content: 39.76

Gene sequence:

>2211_bases
ATGAAGCACCTTATTATCGTAGAATCCCCCGCAAAAGCCAAAACCATTAAAAATTTTTTGGATAAAAATTACGAAGTCAT
CGCCTCTAAAGGGCATGTTAGGGATTTATCCAAATTCGCTTTAGGCATTAAGATTGATGAAACAGGCTTCACTCCTAATT
ATGTCGTGGATAAAGACCATAAAGAGCTTGTCAAACAGATCATAGAGCTTTCTAAAAAGGCATCTACCACCTACATCGCT
ACCGATGAGGACAGAGAAGGGGAAGCGATAGGCTATCATGTGGCATGCTTGATTGGGGGGAAATTAGAGAGCTATCCTAG
GATTGTTTTTCATGAGATCACGCAAAATGCGATCTTAAACGCTCTAAAAACCCCACGAAAAATTGACATGTCTAAGGTCA
ACGCCCAACAAGCCAGACGCTTTTTAGATCGAATCGTGGGTTTTAAGCTCAGTTCGTTGATTGCATCAAAAATCACTAAA
GGTTTGAGCGCTGGGCGGGTGCAAAGCGCGGCTTTAAAGCTTGTGATTGATAAAGAAAGGGAGATCAAAGCCTTTAAGCC
TTTAACCTACTTCACGCTAGACGCTTATTTTGAGTCGCATTTAGAAGCGCAACTCATTAGCTATAAGGGCAACAAACTCA
AAGCCCAAGAACTCATTGATGAAAAAAAAGCTCAAGAGATTAAAAACGAATTAGAAAAAGAAAGCTATATTATTTCCAGT
ATCGTTAAAAAATCTAAAAAATCCCCTACACCGCCCCCTTTTATGACTTCTACTTTACAGCAAAGCGCTTCCAGTCTTTT
AGGCTTTTCGCCCACAAAAACTATGAGTATCGCTCAAAAATTATATGAAGGCGTAGCCACCCCACAAGGGGTTATGGGCG
TGATCACTTACATGAGGACCGATAGCTTGAATATCGCTAAAGAAGCTTTAGAAGAAGCGAGGAATAAGATTTTAAAAGAC
TATGGCAAAGACTACTTACCCCCTAAAGCCAAAGTCTATTCCAGCAAGAATAAAAACGCCCAAGAAGCCCATGAAGCGAT
CAGGCCCACTTCTATTATTTTAGAGCCAAACGCTTTAAAAGACTACCTTAAGCCTGAAGAATTAAAGCTCTATACTTTAA
TTTATAAACGCTTTTTAGCTTCTCAAATGCAAGACGCTCTTTTTGAAAGCCAAAGCGTGGTTGTGGCTTGCGAAAAAGGC
GAGTTTAAAGCCAGTGGCAGAAAACTCCTTTTTGATGGCTATTATAAAATTTTAGGCAATGACGATAAGGACAAATTGCT
CCCTAATTTGAAAGAAAATGACCCCATTAAATTAGAAAAACTAGAGAGCAACGCCCATGTTACAGAACCTCCAGCACGCT
ATTCAGAGGCGAGTTTGATTAAAGTTTTAGAAAGTTTAGGCATAGGCAGGCCCAGCACCTACGCCCCAACGATTTCCCTC
TTACAAAATAGAGATTACATCAAGGTAGAAAAAAAGCAAATCAGCGCTTTAGAGAGCGCGTTTAAGGTGATAGAAATTTT
AGAAAAGCATTTTGAAGAAATCGTGGATTCCAAATTCAGCGCTTCTTTAGAAGAGGAACTGGACAATATCGCTCAAAATA
AAGCCGACTACCAGCAAGTCTTAAAGGACTTTTACTACCCTTTTATGGATAAAATTGAAGCCGGGAAAAAGAATATCATC
TCTCAAAAAGTGCATGAAAAAACCGGCGAGTCATGCCCTAAATGCGGTGGGGAATTAGTCAAAAAAAATAGCCGTTATGG
GGAGTTTATCGCTTGCAACAATTACCCTAAATGCAAATATGTCAAACAGACTGAAAACGCCAATGATGGAGCCAATCAAG
AATTGTGCGAAAAATGCGGAGGGGAAATGGTGCAAAAATTCAGCAGAAACGGGGCGTTTTTAGCTTGCAACAATTACCCT
GAATGCAAAAACACCAAATCGTTAAAAAACACCCCTAACGCAAAAGAAATAATAGAAGGCGTGAAATGCCCAGAATGCGG
GGGGGATATTGCCTTAAAAAGGAGTAGGAAAGGCTCGTTTTATGGCTGCAACAATTACCCTAAATGCAATTTTTTATCCA
ACCATAAGCCTATCAATAAGCGTTGCGAAAAATGCCATTATTTGATGAGTGAAAGAATCTATCGTAAAAAAAAGGTGCAT
GAATGCATCCAATGCAAAGAGCGCGTGTTTTTAGAGGAAGAGAATGGCTAA

Upstream 100 bases:

>100_bases
GAGCGCCGCTATAGTTTTTATGGTTTAATGAATCGTTTAGTCAAAATTCTATGCTACAATCATTCATTAATAGGTATAAA
CATTTTATTAAAGGCGTTCA

Downstream 100 bases:

>100_bases
AGAAAATCTGCCTGTCGTTTTTGGGCCTGTTTTATCCAGGCGTTTTGGGAGGTCTTTGGGCGTGGATCTATCGCCCTCTA
AAAAACAATGCAATTACAAT

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 736; Mature: 736

Protein sequence:

>736_residues
MKHLIIVESPAKAKTIKNFLDKNYEVIASKGHVRDLSKFALGIKIDETGFTPNYVVDKDHKELVKQIIELSKKASTTYIA
TDEDREGEAIGYHVACLIGGKLESYPRIVFHEITQNAILNALKTPRKIDMSKVNAQQARRFLDRIVGFKLSSLIASKITK
GLSAGRVQSAALKLVIDKEREIKAFKPLTYFTLDAYFESHLEAQLISYKGNKLKAQELIDEKKAQEIKNELEKESYIISS
IVKKSKKSPTPPPFMTSTLQQSASSLLGFSPTKTMSIAQKLYEGVATPQGVMGVITYMRTDSLNIAKEALEEARNKILKD
YGKDYLPPKAKVYSSKNKNAQEAHEAIRPTSIILEPNALKDYLKPEELKLYTLIYKRFLASQMQDALFESQSVVVACEKG
EFKASGRKLLFDGYYKILGNDDKDKLLPNLKENDPIKLEKLESNAHVTEPPARYSEASLIKVLESLGIGRPSTYAPTISL
LQNRDYIKVEKKQISALESAFKVIEILEKHFEEIVDSKFSASLEEELDNIAQNKADYQQVLKDFYYPFMDKIEAGKKNII
SQKVHEKTGESCPKCGGELVKKNSRYGEFIACNNYPKCKYVKQTENANDGANQELCEKCGGEMVQKFSRNGAFLACNNYP
ECKNTKSLKNTPNAKEIIEGVKCPECGGDIALKRSRKGSFYGCNNYPKCNFLSNHKPINKRCEKCHYLMSERIYRKKKVH
ECIQCKERVFLEEENG

Sequences:

>Translated_736_residues
MKHLIIVESPAKAKTIKNFLDKNYEVIASKGHVRDLSKFALGIKIDETGFTPNYVVDKDHKELVKQIIELSKKASTTYIA
TDEDREGEAIGYHVACLIGGKLESYPRIVFHEITQNAILNALKTPRKIDMSKVNAQQARRFLDRIVGFKLSSLIASKITK
GLSAGRVQSAALKLVIDKEREIKAFKPLTYFTLDAYFESHLEAQLISYKGNKLKAQELIDEKKAQEIKNELEKESYIISS
IVKKSKKSPTPPPFMTSTLQQSASSLLGFSPTKTMSIAQKLYEGVATPQGVMGVITYMRTDSLNIAKEALEEARNKILKD
YGKDYLPPKAKVYSSKNKNAQEAHEAIRPTSIILEPNALKDYLKPEELKLYTLIYKRFLASQMQDALFESQSVVVACEKG
EFKASGRKLLFDGYYKILGNDDKDKLLPNLKENDPIKLEKLESNAHVTEPPARYSEASLIKVLESLGIGRPSTYAPTISL
LQNRDYIKVEKKQISALESAFKVIEILEKHFEEIVDSKFSASLEEELDNIAQNKADYQQVLKDFYYPFMDKIEAGKKNII
SQKVHEKTGESCPKCGGELVKKNSRYGEFIACNNYPKCKYVKQTENANDGANQELCEKCGGEMVQKFSRNGAFLACNNYP
ECKNTKSLKNTPNAKEIIEGVKCPECGGDIALKRSRKGSFYGCNNYPKCNFLSNHKPINKRCEKCHYLMSERIYRKKKVH
ECIQCKERVFLEEENG
>Mature_736_residues
MKHLIIVESPAKAKTIKNFLDKNYEVIASKGHVRDLSKFALGIKIDETGFTPNYVVDKDHKELVKQIIELSKKASTTYIA
TDEDREGEAIGYHVACLIGGKLESYPRIVFHEITQNAILNALKTPRKIDMSKVNAQQARRFLDRIVGFKLSSLIASKITK
GLSAGRVQSAALKLVIDKEREIKAFKPLTYFTLDAYFESHLEAQLISYKGNKLKAQELIDEKKAQEIKNELEKESYIISS
IVKKSKKSPTPPPFMTSTLQQSASSLLGFSPTKTMSIAQKLYEGVATPQGVMGVITYMRTDSLNIAKEALEEARNKILKD
YGKDYLPPKAKVYSSKNKNAQEAHEAIRPTSIILEPNALKDYLKPEELKLYTLIYKRFLASQMQDALFESQSVVVACEKG
EFKASGRKLLFDGYYKILGNDDKDKLLPNLKENDPIKLEKLESNAHVTEPPARYSEASLIKVLESLGIGRPSTYAPTISL
LQNRDYIKVEKKQISALESAFKVIEILEKHFEEIVDSKFSASLEEELDNIAQNKADYQQVLKDFYYPFMDKIEAGKKNII
SQKVHEKTGESCPKCGGELVKKNSRYGEFIACNNYPKCKYVKQTENANDGANQELCEKCGGEMVQKFSRNGAFLACNNYP
ECKNTKSLKNTPNAKEIIEGVKCPECGGDIALKRSRKGSFYGCNNYPKCNFLSNHKPINKRCEKCHYLMSERIYRKKKVH
ECIQCKERVFLEEENG

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=691, Percent_Identity=22.575976845152, Blast_Score=123, Evalue=8e-28,
Organism=Homo sapiens, GI10835218, Length=595, Percent_Identity=24.0336134453782, Blast_Score=118, Evalue=3e-26,
Organism=Escherichia coli, GI1787529, Length=751, Percent_Identity=37.6830892143808, Blast_Score=457, Evalue=1e-130,
Organism=Escherichia coli, GI48994931, Length=176, Percent_Identity=38.0681818181818, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788061, Length=567, Percent_Identity=23.4567901234568, Blast_Score=114, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32563869, Length=624, Percent_Identity=23.7179487179487, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17555378, Length=466, Percent_Identity=26.6094420600858, Blast_Score=120, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6323263, Length=571, Percent_Identity=23.4676007005254, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24585251, Length=633, Percent_Identity=25.434439178515, Blast_Score=123, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24640096, Length=544, Percent_Identity=21.5073529411765, Blast_Score=89, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 83236; Mature: 83236

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHLIIVESPAKAKTIKNFLDKNYEVIASKGHVRDLSKFALGIKIDETGFTPNYVVDKDH
CCCEEEEECCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHEEEEEECCCCCCCCEECCHH
KELVKQIIELSKKASTTYIATDEDREGEAIGYHVACLIGGKLESYPRIVFHEITQNAILN
HHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEEEEECCCHHHCCHHHHHHHHHHHHHH
ALKTPRKIDMSKVNAQQARRFLDRIVGFKLSSLIASKITKGLSAGRVQSAALKLVIDKER
HHCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCHHHHHHHEEEECCCH
EIKAFKPLTYFTLDAYFESHLEAQLISYKGNKLKAQELIDEKKAQEIKNELEKESYIISS
HHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVKKSKKSPTPPPFMTSTLQQSASSLLGFSPTKTMSIAQKLYEGVATPQGVMGVITYMRT
HHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHC
DSLNIAKEALEEARNKILKDYGKDYLPPKAKVYSSKNKNAQEAHEAIRPTSIILEPNALK
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHCCCEEEECCHHHH
DYLKPEELKLYTLIYKRFLASQMQDALFESQSVVVACEKGEFKASGRKLLFDGYYKILGN
HHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEHHHHHHCCC
DDKDKLLPNLKENDPIKLEKLESNAHVTEPPARYSEASLIKVLESLGIGRPSTYAPTISL
CCHHHHCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHH
LQNRDYIKVEKKQISALESAFKVIEILEKHFEEIVDSKFSASLEEELDNIAQNKADYQQV
HCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
LKDFYYPFMDKIEAGKKNIISQKVHEKTGESCPKCGGELVKKNSRYGEFIACNNYPKCKY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEECCCCCCCE
VKQTENANDGANQELCEKCGGEMVQKFSRNGAFLACNNYPECKNTKSLKNTPNAKEIIEG
EECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHCC
VKCPECGGDIALKRSRKGSFYGCNNYPKCNFLSNHKPINKRCEKCHYLMSERIYRKKKVH
CCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ECIQCKERVFLEEENG
HHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKHLIIVESPAKAKTIKNFLDKNYEVIASKGHVRDLSKFALGIKIDETGFTPNYVVDKDH
CCCEEEEECCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHEEEEEECCCCCCCCEECCHH
KELVKQIIELSKKASTTYIATDEDREGEAIGYHVACLIGGKLESYPRIVFHEITQNAILN
HHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEEEEECCCHHHCCHHHHHHHHHHHHHH
ALKTPRKIDMSKVNAQQARRFLDRIVGFKLSSLIASKITKGLSAGRVQSAALKLVIDKER
HHCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCHHHHHHHEEEECCCH
EIKAFKPLTYFTLDAYFESHLEAQLISYKGNKLKAQELIDEKKAQEIKNELEKESYIISS
HHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVKKSKKSPTPPPFMTSTLQQSASSLLGFSPTKTMSIAQKLYEGVATPQGVMGVITYMRT
HHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHC
DSLNIAKEALEEARNKILKDYGKDYLPPKAKVYSSKNKNAQEAHEAIRPTSIILEPNALK
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHCCCEEEECCHHHH
DYLKPEELKLYTLIYKRFLASQMQDALFESQSVVVACEKGEFKASGRKLLFDGYYKILGN
HHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEHHHHHHCCC
DDKDKLLPNLKENDPIKLEKLESNAHVTEPPARYSEASLIKVLESLGIGRPSTYAPTISL
CCHHHHCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHH
LQNRDYIKVEKKQISALESAFKVIEILEKHFEEIVDSKFSASLEEELDNIAQNKADYQQV
HCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
LKDFYYPFMDKIEAGKKNIISQKVHEKTGESCPKCGGELVKKNSRYGEFIACNNYPKCKY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEECCCCCCCE
VKQTENANDGANQELCEKCGGEMVQKFSRNGAFLACNNYPECKNTKSLKNTPNAKEIIEG
EECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHCC
VKCPECGGDIALKRSRKGSFYGCNNYPKCNFLSNHKPINKRCEKCHYLMSERIYRKKKVH
CCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ECIQCKERVFLEEENG
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]