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The map label for this gene is metB [H]

Identifier: 108562531

GI number: 108562531

Start: 110879

End: 112021

Strand: Reverse

Name: metB [H]

Synonym: HPAG1_0106

Alternate gene names: 108562531

Gene position: 112021-110879 (Counterclockwise)

Preceding gene: 108562532

Following gene: 108562530

Centisome position: 7.02

GC content: 46.63

Gene sequence:

>1143_bases
ATGCGCATGCAAACAAAACTAATCCATGGGGGCATTAGTGAGGATACGACAACGGGGGCGGTGAGCGTGCCTATTTATCA
AACTTCCACCTACCGCCAAGACGCCATAGGCCACCATAAGGGTTATGAATACTCTCGCTCAGGCAACCCCACACGCTTTG
CTTTAGAAGAACTCATCGCCGATTTAGAAGGGGGGGTTAAGGGGTTTGCTTTTGCCTCTGGACTAGCTGGGATCCACGCT
GTTTTTTCCCTTTTACAATCAGGCGATCATGTGTTATTGGGCGATGATGTTTATGGGGGGACTTTTCGCTTGTTTAATAA
AGTGCTTGTCAAAAATGGGCTTTCTTGCACCATCATAGACACTAGCGATCTATCCCAAATCAAAAAGGCTATCAAGCAAA
ACACCAAAGCCCTTTATTTAGAAACCCCTAGTAACCCCTTGCTTAAAATCACGGATTTAGCGCAATGCGCGAGTGTTGCT
AAAGATCATGGTTTGCTCACTATCGTGGATAACACCTTTGCCACCCCCTATTATCAAAACCCGCTTCTTTTGGGAGCGGA
TATTGTGGTGCATAGCGGCACGAAATACTTAGGCGGGCATAGCGATGTGGTCGCTGGGCTTGTAACCACTAATAATGAAG
CGCTATCCCAAGAGATCGCTTTTTTCCAAAACGCTATCGGTGGGGTTTTAGGCCCTCAAGACAGCTGGCTGTTGCAAAGA
GGGATTAAAACGCTAGCATTACGCATGGAGGCCCATCAAAAAAACGCTCTTTGCGTGGCTGAGTTTTTAGAAAAACACCC
TAAAGTAGAAAAGGTTTATTACCCGGGCTTGCCCACCCACCCTAATTACGAGCTAGCCAAAGCCCAAATGCGTGGCTTTA
GCGGGATGCTCTCTTTCACTCTCAAAAATGACAGCGAGGCGACTCTTTTTGTAGAAAGCCTTAAATTATTCATTTTAGGC
GAGAGTTTGGGCGGGGTGGAAAGTTTGGTAGGGATTCCGGCATTTATGACCCATGCGTGTATCCCTAAAGAACAACGAGA
AGCTGCAGGGATTAGAGATGGCTTGGTGCGCTTGTCTGTGGGGATTGAGCATGAACAGGATTTGTTAGAAGATTTAGAGC
AAGCGTTCGCTAAAATAGGCTAA

Upstream 100 bases:

>100_bases
GCCTCCCCGAAGGCAGCCAGGTTTTAACCATTTTCCCGGATGTTGCCGATCGTTATCTTTCAAAAGGTATTTACTTATAA
ATTCATCAAAAGGAAAAATC

Downstream 100 bases:

>100_bases
AGTTTCATTACAATTTATGAATGAAGGAGTTAAAAACATGAAAACGCCAAAAATGAATGTAGAAAGTTTTAATTTGGATC
ACACCAAAGTCAAAGCCCCT

Product: cystathionine gamma-synthase/cystathionine beta-lyase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MRMQTKLIHGGISEDTTTGAVSVPIYQTSTYRQDAIGHHKGYEYSRSGNPTRFALEELIADLEGGVKGFAFASGLAGIHA
VFSLLQSGDHVLLGDDVYGGTFRLFNKVLVKNGLSCTIIDTSDLSQIKKAIKQNTKALYLETPSNPLLKITDLAQCASVA
KDHGLLTIVDNTFATPYYQNPLLLGADIVVHSGTKYLGGHSDVVAGLVTTNNEALSQEIAFFQNAIGGVLGPQDSWLLQR
GIKTLALRMEAHQKNALCVAEFLEKHPKVEKVYYPGLPTHPNYELAKAQMRGFSGMLSFTLKNDSEATLFVESLKLFILG
ESLGGVESLVGIPAFMTHACIPKEQREAAGIRDGLVRLSVGIEHEQDLLEDLEQAFAKIG

Sequences:

>Translated_380_residues
MRMQTKLIHGGISEDTTTGAVSVPIYQTSTYRQDAIGHHKGYEYSRSGNPTRFALEELIADLEGGVKGFAFASGLAGIHA
VFSLLQSGDHVLLGDDVYGGTFRLFNKVLVKNGLSCTIIDTSDLSQIKKAIKQNTKALYLETPSNPLLKITDLAQCASVA
KDHGLLTIVDNTFATPYYQNPLLLGADIVVHSGTKYLGGHSDVVAGLVTTNNEALSQEIAFFQNAIGGVLGPQDSWLLQR
GIKTLALRMEAHQKNALCVAEFLEKHPKVEKVYYPGLPTHPNYELAKAQMRGFSGMLSFTLKNDSEATLFVESLKLFILG
ESLGGVESLVGIPAFMTHACIPKEQREAAGIRDGLVRLSVGIEHEQDLLEDLEQAFAKIG
>Mature_380_residues
MRMQTKLIHGGISEDTTTGAVSVPIYQTSTYRQDAIGHHKGYEYSRSGNPTRFALEELIADLEGGVKGFAFASGLAGIHA
VFSLLQSGDHVLLGDDVYGGTFRLFNKVLVKNGLSCTIIDTSDLSQIKKAIKQNTKALYLETPSNPLLKITDLAQCASVA
KDHGLLTIVDNTFATPYYQNPLLLGADIVVHSGTKYLGGHSDVVAGLVTTNNEALSQEIAFFQNAIGGVLGPQDSWLLQR
GIKTLALRMEAHQKNALCVAEFLEKHPKVEKVYYPGLPTHPNYELAKAQMRGFSGMLSFTLKNDSEATLFVESLKLFILG
ESLGGVESLVGIPAFMTHACIPKEQREAAGIRDGLVRLSVGIEHEQDLLEDLEQAFAKIG

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=360, Percent_Identity=49.7222222222222, Blast_Score=358, Evalue=6e-99,
Organism=Homo sapiens, GI299473759, Length=360, Percent_Identity=45, Blast_Score=308, Evalue=7e-84,
Organism=Homo sapiens, GI25453487, Length=359, Percent_Identity=44.5682451253482, Blast_Score=292, Evalue=4e-79,
Organism=Escherichia coli, GI1790375, Length=378, Percent_Identity=42.5925925925926, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI1789383, Length=399, Percent_Identity=30.8270676691729, Blast_Score=188, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI17538055, Length=364, Percent_Identity=48.9010989010989, Blast_Score=353, Evalue=8e-98,
Organism=Caenorhabditis elegans, GI17560056, Length=364, Percent_Identity=48.3516483516484, Blast_Score=350, Evalue=5e-97,
Organism=Caenorhabditis elegans, GI71980708, Length=370, Percent_Identity=41.8918918918919, Blast_Score=285, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6319307, Length=387, Percent_Identity=47.2868217054264, Blast_Score=330, Evalue=2e-91,
Organism=Saccharomyces cerevisiae, GI6321254, Length=402, Percent_Identity=33.5820895522388, Blast_Score=202, Evalue=6e-53,
Organism=Saccharomyces cerevisiae, GI6323333, Length=421, Percent_Identity=33.2541567695962, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6321170, Length=330, Percent_Identity=36.6666666666667, Blast_Score=183, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6321904, Length=395, Percent_Identity=29.3670886075949, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6322970, Length=363, Percent_Identity=27.5482093663912, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323557, Length=343, Percent_Identity=27.1137026239067, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6322590, Length=276, Percent_Identity=29.3478260869565, Blast_Score=83, Evalue=9e-17,
Organism=Drosophila melanogaster, GI19922554, Length=378, Percent_Identity=50.2645502645503, Blast_Score=378, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 41206; Mature: 41206

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMQTKLIHGGISEDTTTGAVSVPIYQTSTYRQDAIGHHKGYEYSRSGNPTRFALEELIA
CCCCHHHHCCCCCCCCCCCEEEEEEEECCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
DLEGGVKGFAFASGLAGIHAVFSLLQSGDHVLLGDDVYGGTFRLFNKVLVKNGLSCTIID
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCEEEEEC
TSDLSQIKKAIKQNTKALYLETPSNPLLKITDLAQCASVAKDHGLLTIVDNTFATPYYQN
CCCHHHHHHHHHCCCCEEEEECCCCCCEEHHHHHHHHHHHHCCCEEEEECCCCCCCCCCC
PLLLGADIVVHSGTKYLGGHSDVVAGLVTTNNEALSQEIAFFQNAIGGVLGPQDSWLLQR
CEEEECEEEEECCCCCCCCCHHHEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHH
GIKTLALRMEAHQKNALCVAEFLEKHPKVEKVYYPGLPTHPNYELAKAQMRGFSGMLSFT
HHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEE
LKNDSEATLFVESLKLFILGESLGGVESLVGIPAFMTHACIPKEQREAAGIRDGLVRLSV
ECCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHCCHHHCEEEEEE
GIEHEQDLLEDLEQAFAKIG
CCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRMQTKLIHGGISEDTTTGAVSVPIYQTSTYRQDAIGHHKGYEYSRSGNPTRFALEELIA
CCCCHHHHCCCCCCCCCCCEEEEEEEECCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
DLEGGVKGFAFASGLAGIHAVFSLLQSGDHVLLGDDVYGGTFRLFNKVLVKNGLSCTIID
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCEEEEEC
TSDLSQIKKAIKQNTKALYLETPSNPLLKITDLAQCASVAKDHGLLTIVDNTFATPYYQN
CCCHHHHHHHHHCCCCEEEEECCCCCCEEHHHHHHHHHHHHCCCEEEEECCCCCCCCCCC
PLLLGADIVVHSGTKYLGGHSDVVAGLVTTNNEALSQEIAFFQNAIGGVLGPQDSWLLQR
CEEEECEEEEECCCCCCCCCHHHEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHH
GIKTLALRMEAHQKNALCVAEFLEKHPKVEKVYYPGLPTHPNYELAKAQMRGFSGMLSFT
HHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEE
LKNDSEATLFVESLKLFILGESLGGVESLVGIPAFMTHACIPKEQREAAGIRDGLVRLSV
ECCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHCCHHHCEEEEEE
GIEHEQDLLEDLEQAFAKIG
CCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]