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The map label for this gene is pucI [H]

Identifier: 107023145

GI number: 107023145

Start: 1768910

End: 1770418

Strand: Reverse

Name: pucI [H]

Synonym: Bcen_1594

Alternate gene names: 107023145

Gene position: 1770418-1768910 (Counterclockwise)

Preceding gene: 107023146

Following gene: 107023144

Centisome position: 53.74

GC content: 66.93

Gene sequence:

>1509_bases
ATGAAACCAGCAGCGATTCCCGCCGATCCCGACAGCGCCGCGGCGCACGGCGGCAGCCTGTACAACGACGACCTCGCGCC
GACGACGCCGGCGCAGCGCACGTGGAAGTGGTACCACTTCGCGGCGCTGTGGGTCGGGATGGTGATGAACATCGCGTCGT
ACATGCTCGCGGCCGGCTTGATCCAGGAAGGCATGTCGCCGTGGCAGGCGGTGATGACGGTGCTGCTCGGCAACCTGATC
GTGCTCGTGCCGATGCTGTTGATCGGCCATGCAGGCGCGAAGCACGGGATTCCGTATGCGGTGCTCGTGCGCGCGTCGTT
CGGTACGCAGGGCGCGAAGCTGCCCGCGCTGCTGCGCGCGATCGTCGCCTGCGGCTGGTACGGCATCCAGACCTGGCTCG
GCGGCAGCGCGATCTACACGCTGCTGAACATCCTGACCGGCAACGCGCTGCACGGCGCGGCGCTGCCTGTCGTTGGCATC
TCGTTCGGGCAACTCGCGTGCTTCCTCGTGTTCTGGGCGCTGCAGCTGTATTTCATCTGGCACGGCACCGATTCGATCCG
CTGGCTCGAAAGCTGGTCTGCGCCGATCAAGGTCGTGATGTGCGTGGCGCTCGTGTGGTGGGCGACGTCGCAGGCGGGCG
GTGTCGGCTCGATGCTGTCGGCGCCGTCGCAGTTCGCGGCGGGCGGCAAGAAGGCCGGACTCTTCTGGGCGACCTTCTGG
CCGGGCCTGACCGCGATGGTCGGCTTCTGGGCGACGCTCGCGCTGAACATTCCCGATTTCACGCGCTTCGCGCATTCGCA
GCGCGACCAGATGATCGGGCAGTCGATCGGGCTGCCGCTGCCGATGGCGCTGCTGTCGGTGGTGTCGGTCGTCGTGACGT
CGGCGACCGTCGTGATCTACGGCAACGCGATCTGGGATCCGATCGACCTGACGAGCCGGATGACCGGCATCGGCGTGGGC
ATCGCGCTCGTGATCCTCACGCTCGACACGATGTGCTGCAACCTCGCGGCGAACCTGGTCGGTCCCGCATACGATTTTTC
GAGCCTGTGGCCGAAGGCGATCTCGTACCGCGCGGGCGGGATGATCACCGCGACGATCGCGATCGTGATGATGCCGTGGA
AGATCCTCGCGACGACGGACGGCTACATCTTCACCTGGCTCGTCGGTTATTCGGCGCTGCTCGGGCCGGTCGCGGGCATC
CTGATGGTCGACTATTTCCTGATTCGCGGCACGCAGCTCGACACACGCGCACTGTTCGACGAGCGCGGCGACTTCAGCTA
TGCGCGCGGCTGGAACCCGGCCGCGCTGGTCGCGCTCGCGGTCGGCGTGCTGCCGAACCTGCCGGGCTTCCTGCATACGG
CGTTTCCGGCGTCGTTCCCGAACGTGCCGGCGTTCTTCAACACGCTTTACACGTACGCGTGGTTCGTCGGCCTCGTGCTG
GCGTCGGGCGTGTACGGCACCTGGATGAAGTGGCGCGCCGGACAGCGCGCGCAGATCGCGAGCGCATGA

Upstream 100 bases:

>100_bases
CCGAGGTCCGGCGGTGCGGCATGGACCGCGCCGCCGCTTTTCGTTTCCCCGGGCCGGCATCGCACGATGCCGGCGCCGAC
GTTTTCGTGGAGCGCGTTCA

Downstream 100 bases:

>100_bases
CGCACGCGCGGGCCGGATGCGGCTCGCGCGCGGCATGCGGCACCGACAGTCAACGAGGAGGCAACGAAATGGCAATCCTG
ATTCGTGGCGGCACCGTGGT

Product: NCS1 nucleoside transporter

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MKPAAIPADPDSAAAHGGSLYNDDLAPTTPAQRTWKWYHFAALWVGMVMNIASYMLAAGLIQEGMSPWQAVMTVLLGNLI
VLVPMLLIGHAGAKHGIPYAVLVRASFGTQGAKLPALLRAIVACGWYGIQTWLGGSAIYTLLNILTGNALHGAALPVVGI
SFGQLACFLVFWALQLYFIWHGTDSIRWLESWSAPIKVVMCVALVWWATSQAGGVGSMLSAPSQFAAGGKKAGLFWATFW
PGLTAMVGFWATLALNIPDFTRFAHSQRDQMIGQSIGLPLPMALLSVVSVVVTSATVVIYGNAIWDPIDLTSRMTGIGVG
IALVILTLDTMCCNLAANLVGPAYDFSSLWPKAISYRAGGMITATIAIVMMPWKILATTDGYIFTWLVGYSALLGPVAGI
LMVDYFLIRGTQLDTRALFDERGDFSYARGWNPAALVALAVGVLPNLPGFLHTAFPASFPNVPAFFNTLYTYAWFVGLVL
ASGVYGTWMKWRAGQRAQIASA

Sequences:

>Translated_502_residues
MKPAAIPADPDSAAAHGGSLYNDDLAPTTPAQRTWKWYHFAALWVGMVMNIASYMLAAGLIQEGMSPWQAVMTVLLGNLI
VLVPMLLIGHAGAKHGIPYAVLVRASFGTQGAKLPALLRAIVACGWYGIQTWLGGSAIYTLLNILTGNALHGAALPVVGI
SFGQLACFLVFWALQLYFIWHGTDSIRWLESWSAPIKVVMCVALVWWATSQAGGVGSMLSAPSQFAAGGKKAGLFWATFW
PGLTAMVGFWATLALNIPDFTRFAHSQRDQMIGQSIGLPLPMALLSVVSVVVTSATVVIYGNAIWDPIDLTSRMTGIGVG
IALVILTLDTMCCNLAANLVGPAYDFSSLWPKAISYRAGGMITATIAIVMMPWKILATTDGYIFTWLVGYSALLGPVAGI
LMVDYFLIRGTQLDTRALFDERGDFSYARGWNPAALVALAVGVLPNLPGFLHTAFPASFPNVPAFFNTLYTYAWFVGLVL
ASGVYGTWMKWRAGQRAQIASA
>Mature_502_residues
MKPAAIPADPDSAAAHGGSLYNDDLAPTTPAQRTWKWYHFAALWVGMVMNIASYMLAAGLIQEGMSPWQAVMTVLLGNLI
VLVPMLLIGHAGAKHGIPYAVLVRASFGTQGAKLPALLRAIVACGWYGIQTWLGGSAIYTLLNILTGNALHGAALPVVGI
SFGQLACFLVFWALQLYFIWHGTDSIRWLESWSAPIKVVMCVALVWWATSQAGGVGSMLSAPSQFAAGGKKAGLFWATFW
PGLTAMVGFWATLALNIPDFTRFAHSQRDQMIGQSIGLPLPMALLSVVSVVVTSATVVIYGNAIWDPIDLTSRMTGIGVG
IALVILTLDTMCCNLAANLVGPAYDFSSLWPKAISYRAGGMITATIAIVMMPWKILATTDGYIFTWLVGYSALLGPVAGI
LMVDYFLIRGTQLDTRALFDERGDFSYARGWNPAALVALAVGVLPNLPGFLHTAFPASFPNVPAFFNTLYTYAWFVGLVL
ASGVYGTWMKWRAGQRAQIASA

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=483, Percent_Identity=29.6066252587992, Blast_Score=206, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6319429, Length=481, Percent_Identity=27.6507276507277, Blast_Score=191, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6322219, Length=451, Percent_Identity=27.7161862527716, Blast_Score=187, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6319495, Length=450, Percent_Identity=26.6666666666667, Blast_Score=179, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6324645, Length=463, Percent_Identity=24.1900647948164, Blast_Score=144, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6323266, Length=449, Percent_Identity=23.3853006681514, Blast_Score=131, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6324766, Length=465, Percent_Identity=22.7956989247312, Blast_Score=127, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 53855; Mature: 53855

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPAAIPADPDSAAAHGGSLYNDDLAPTTPAQRTWKWYHFAALWVGMVMNIASYMLAAGL
CCCCCCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IQEGMSPWQAVMTVLLGNLIVLVPMLLIGHAGAKHGIPYAVLVRASFGTQGAKLPALLRA
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHHHH
IVACGWYGIQTWLGGSAIYTLLNILTGNALHGAALPVVGISFGQLACFLVFWALQLYFIW
HHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHE
HGTDSIRWLESWSAPIKVVMCVALVWWATSQAGGVGSMLSAPSQFAAGGKKAGLFWATFW
ECCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHCCCCCCCEEEEHHH
PGLTAMVGFWATLALNIPDFTRFAHSQRDQMIGQSIGLPLPMALLSVVSVVVTSATVVIY
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCEEEEE
GNAIWDPIDLTSRMTGIGVGIALVILTLDTMCCNLAANLVGPAYDFSSLWPKAISYRAGG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
MITATIAIVMMPWKILATTDGYIFTWLVGYSALLGPVAGILMVDYFLIRGTQLDTRALFD
HHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
ERGDFSYARGWNPAALVALAVGVLPNLPGFLHTAFPASFPNVPAFFNTLYTYAWFVGLVL
CCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
ASGVYGTWMKWRAGQRAQIASA
HCCCHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKPAAIPADPDSAAAHGGSLYNDDLAPTTPAQRTWKWYHFAALWVGMVMNIASYMLAAGL
CCCCCCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IQEGMSPWQAVMTVLLGNLIVLVPMLLIGHAGAKHGIPYAVLVRASFGTQGAKLPALLRA
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHHHH
IVACGWYGIQTWLGGSAIYTLLNILTGNALHGAALPVVGISFGQLACFLVFWALQLYFIW
HHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHE
HGTDSIRWLESWSAPIKVVMCVALVWWATSQAGGVGSMLSAPSQFAAGGKKAGLFWATFW
ECCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHCCCCCCCEEEEHHH
PGLTAMVGFWATLALNIPDFTRFAHSQRDQMIGQSIGLPLPMALLSVVSVVVTSATVVIY
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCEEEEE
GNAIWDPIDLTSRMTGIGVGIALVILTLDTMCCNLAANLVGPAYDFSSLWPKAISYRAGG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
MITATIAIVMMPWKILATTDGYIFTWLVGYSALLGPVAGILMVDYFLIRGTQLDTRALFD
HHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
ERGDFSYARGWNPAALVALAVGVLPNLPGFLHTAFPASFPNVPAFFNTLYTYAWFVGLVL
CCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
ASGVYGTWMKWRAGQRAQIASA
HCCCHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]