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The map label for this gene is miaA [H]

Identifier: 107021840

GI number: 107021840

Start: 306170

End: 307144

Strand: Reverse

Name: miaA [H]

Synonym: Bcen_0280

Alternate gene names: 107021840

Gene position: 307144-306170 (Counterclockwise)

Preceding gene: 107021841

Following gene: 107021838

Centisome position: 9.32

GC content: 71.69

Gene sequence:

>975_bases
ATGAGCGCTTCTTCGCAGTCCCGCCCGACGACGATCGCCTGCCTGCTCGGCCCCACCGCTTCGGGCAAGACGGCCGCGGC
GCTGGCGCTCGCCGCGCGCCGCCCGATCGAGATCGTCAGCGTCGATTCGGCGCTCGTCTACCGCGACATGGATATCGGCA
CCGCGAAACCGTCGCGCGAGGAGCGTGCGAGCGTGCCGCATCACCTGATCGACATCATCGACCCGGCGGACGCGTATTCG
GCCGCGGAATTCCGCGCCGACACGCTCCGGCTGATCGGCGAGATCGTCGCGCGGGGCCGCACGCCGCTGCTCGCCGGCGG
CACGATGCTGTACTACAAGGCGCTGACGCAAGGGCTCAACGACTTGCCGGGCGCCGACCCGGAAGTCCGCGCGGCACTCG
ACGCCGACGCCGCGCGCGACGGCTGGCCCGCGCTGCACGCGCGGCTCGCGCAGGTCGACCCCGACACGGCCGCGCGGCTC
GCGCCGAACGATTCGCAGCGAATCCAGCGTGCACTGGAAATCTTCATGCTGAGCGGGCAGCCGATGTCGGCGCTGCTCGC
CGCGCCGCGCCGCACCGACGATGCGGCCGCCGCGTACCGCTTCGTGCCGGTCGCGCTCGAGCCGTCGGACCGCGCGGTGC
TGCACACGCGGATCGCGCAGCGCTTCGACGCGATGCTCGATGCGGGCTTCATCGACGAAGTCGAACGGCTGCGGCGCCGC
GACGATCTCCATCCCGACCTGCCGTCGATGCGGTGCGTCGGCTACCGCCAGGCGTGGGAATACCTCGACGGCGATACCGA
CTACCGGACGATGCGCGACAAGGGCATCTTCGCGACCCGCCAGCTGTGCAAGCGCCAGATCACATGGCTGCGCGCGATGC
CGGAGCGGATCGTGGTCGACTGCATCGCGCCGGATGCGACGGCCCGCGCGCTCGACGCGCTCGAACGCGTGCTCGACGGC
CGCACGCCGGACTAG

Upstream 100 bases:

>100_bases
AGATGGAGGCGACCGAGCGCGCGGACCAGTGCAATCACGGCCGGCCGACCTGGTATCAGCTCACGCTGAACGATCTCGAC
CGTCTCTTCATGCGCGGGCA

Downstream 100 bases:

>100_bases
CCGCTCAGCCCGCCCGACGCGCCGCAAGGCGCGCGCGCGCGCCGTCGACGATCCATGCGATACCGCGCTCGAACGCTTCG
TCGCCGCTGCGCTCGCGCAG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MSASSQSRPTTIACLLGPTASGKTAAALALAARRPIEIVSVDSALVYRDMDIGTAKPSREERASVPHHLIDIIDPADAYS
AAEFRADTLRLIGEIVARGRTPLLAGGTMLYYKALTQGLNDLPGADPEVRAALDADAARDGWPALHARLAQVDPDTAARL
APNDSQRIQRALEIFMLSGQPMSALLAAPRRTDDAAAAYRFVPVALEPSDRAVLHTRIAQRFDAMLDAGFIDEVERLRRR
DDLHPDLPSMRCVGYRQAWEYLDGDTDYRTMRDKGIFATRQLCKRQITWLRAMPERIVVDCIAPDATARALDALERVLDG
RTPD

Sequences:

>Translated_324_residues
MSASSQSRPTTIACLLGPTASGKTAAALALAARRPIEIVSVDSALVYRDMDIGTAKPSREERASVPHHLIDIIDPADAYS
AAEFRADTLRLIGEIVARGRTPLLAGGTMLYYKALTQGLNDLPGADPEVRAALDADAARDGWPALHARLAQVDPDTAARL
APNDSQRIQRALEIFMLSGQPMSALLAAPRRTDDAAAAYRFVPVALEPSDRAVLHTRIAQRFDAMLDAGFIDEVERLRRR
DDLHPDLPSMRCVGYRQAWEYLDGDTDYRTMRDKGIFATRQLCKRQITWLRAMPERIVVDCIAPDATARALDALERVLDG
RTPD
>Mature_323_residues
SASSQSRPTTIACLLGPTASGKTAAALALAARRPIEIVSVDSALVYRDMDIGTAKPSREERASVPHHLIDIIDPADAYSA
AEFRADTLRLIGEIVARGRTPLLAGGTMLYYKALTQGLNDLPGADPEVRAALDADAARDGWPALHARLAQVDPDTAARLA
PNDSQRIQRALEIFMLSGQPMSALLAAPRRTDDAAAAYRFVPVALEPSDRAVLHTRIAQRFDAMLDAGFIDEVERLRRRD
DLHPDLPSMRCVGYRQAWEYLDGDTDYRTMRDKGIFATRQLCKRQITWLRAMPERIVVDCIAPDATARALDALERVLDGR
TPD

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=315, Percent_Identity=32.3809523809524, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1790613, Length=302, Percent_Identity=53.3112582781457, Blast_Score=311, Evalue=4e-86,
Organism=Caenorhabditis elegans, GI25144712, Length=281, Percent_Identity=28.4697508896797, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324848, Length=294, Percent_Identity=27.5510204081633, Blast_Score=134, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24649830, Length=272, Percent_Identity=29.7794117647059, Blast_Score=121, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 35554; Mature: 35423

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASSQSRPTTIACLLGPTASGKTAAALALAARRPIEIVSVDSALVYRDMDIGTAKPSRE
CCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCEEECCCCCCCCCCHH
ERASVPHHLIDIIDPADAYSAAEFRADTLRLIGEIVARGRTPLLAGGTMLYYKALTQGLN
HHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHH
DLPGADPEVRAALDADAARDGWPALHARLAQVDPDTAARLAPNDSQRIQRALEIFMLSGQ
HCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHCCCC
PMSALLAAPRRTDDAAAAYRFVPVALEPSDRAVLHTRIAQRFDAMLDAGFIDEVERLRRR
CHHHHHHCCCCCCHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
DDLHPDLPSMRCVGYRQAWEYLDGDTDYRTMRDKGIFATRQLCKRQITWLRAMPERIVVD
CCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHEEE
CIAPDATARALDALERVLDGRTPD
ECCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SASSQSRPTTIACLLGPTASGKTAAALALAARRPIEIVSVDSALVYRDMDIGTAKPSRE
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCEEECCCCCCCCCCHH
ERASVPHHLIDIIDPADAYSAAEFRADTLRLIGEIVARGRTPLLAGGTMLYYKALTQGLN
HHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHH
DLPGADPEVRAALDADAARDGWPALHARLAQVDPDTAARLAPNDSQRIQRALEIFMLSGQ
HCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHCCCC
PMSALLAAPRRTDDAAAAYRFVPVALEPSDRAVLHTRIAQRFDAMLDAGFIDEVERLRRR
CHHHHHHCCCCCCHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
DDLHPDLPSMRCVGYRQAWEYLDGDTDYRTMRDKGIFATRQLCKRQITWLRAMPERIVVD
CCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHEEE
CIAPDATARALDALERVLDGRTPD
ECCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA