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The map label for this gene is queA [H]

Identifier: 107021799

GI number: 107021799

Start: 255280

End: 256347

Strand: Reverse

Name: queA [H]

Synonym: Bcen_0239

Alternate gene names: 107021799

Gene position: 256347-255280 (Counterclockwise)

Preceding gene: 107021806

Following gene: 107021798

Centisome position: 7.78

GC content: 69.85

Gene sequence:

>1068_bases
ATGTTCACGCTTTCCGATTTCGATTTCAATCTACCGCCCGAGCTGATTGCCCAAACCGCGCTGCCCGATCGCACCGCGAG
CCGCCTGCTCGAGGTCGACGGCAGCGTCGCGCCCGCACGCCTCGTCGACCGCCATTTCGCCGAGCTGCCGTCGTGCATCG
CGCCGGGCGACCTGCTCGTGTTCAACGATACCAAGGTGCTGAAGGCGCGTTTCTTCGGCCAGAAGGCCAGCGGCGGCAAG
ATCGAGGTGCTGATCGAGCGCGTGACCGGCACGCACACGGCGCTCGCGCAGATCCGCGCGAGCAAGTCGCCGGGCGCCGG
CACGACGCTGCGCCTGGCCGACGCGTTCGACGTGACGGTCGGCGAGCGCGTCGAGCCGTTCTTCACGCTGCACTTCCCCG
CGCCGTGCCTCGACCTGATCGAGCAGCACGGCCGGCTGCCGCTGCCGCCGTATATCGAGCACGACGCCGATGCGACCGAC
GAGACGCGCTACCAGACCGTCTATGCGAGCAACCCGGGCGCGGTCGCCGCGCCCACGGCCGGGCTCCATTTCGACCAGCC
GCTGCTGGATAAGCTCGACGCGATGGGCGTCGAGCGCGCGACGCTCACGCTGCACGTCGGCGCCGGCACGTTCCAGCCGG
TGCGCGTCGAGAACATCGCCGAGCACAAGATGCACAGCGAGTGGTACGACCTGCCGCAGTCGCTGGTCGACAAGATCGCC
GCGACCCGCGCGCGCGGCGGCAACGTGATCGCGGTCGGCACGACGTCGATGCGTGCGCTCGAAGCCGCGGCGCGCAGTGC
CGACGAGGCCGGCCGCCCGCTCGCGGCGACGCAGGCCGAGACCGACATCTTCATCACGCCCGGCTACCGTTTCCGCGTGG
TCGACCGGCTCGTCACGAACTTCCACCTGCCGAAGTCGACGCTGCTGATGCTGGTGTCCGCGTTCGCGGGCGTCGAGACG
ATCCGCGCCGCGTACCGGCACGCGATCGAAGAACGCTACCGCTTCTTCAGCTACGGCGACGCGATGCTGCTCACGCGGCG
CGACACGCCGGAGGCGCCGGGCGCGTAA

Upstream 100 bases:

>100_bases
CGGCCAGGCCGCGCATCCCGTCATCATAGCGGCTCGCGCGCCCCGATTACGCGTTTCCGCCCTACCGGCGACGCCCGCCG
GCCGCTTCGAACCAGCCCGC

Downstream 100 bases:

>100_bases
GCGCAGCCGACGGCATCGGCTCGATGCGCACGATGGCCGGCGCGTCGCACTGCCGCCGTGCCGTTTTTTACGTTGTGCGC
GCTACGCTGGCGCGCACCCG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MFTLSDFDFNLPPELIAQTALPDRTASRLLEVDGSVAPARLVDRHFAELPSCIAPGDLLVFNDTKVLKARFFGQKASGGK
IEVLIERVTGTHTALAQIRASKSPGAGTTLRLADAFDVTVGERVEPFFTLHFPAPCLDLIEQHGRLPLPPYIEHDADATD
ETRYQTVYASNPGAVAAPTAGLHFDQPLLDKLDAMGVERATLTLHVGAGTFQPVRVENIAEHKMHSEWYDLPQSLVDKIA
ATRARGGNVIAVGTTSMRALEAAARSADEAGRPLAATQAETDIFITPGYRFRVVDRLVTNFHLPKSTLLMLVSAFAGVET
IRAAYRHAIEERYRFFSYGDAMLLTRRDTPEAPGA

Sequences:

>Translated_355_residues
MFTLSDFDFNLPPELIAQTALPDRTASRLLEVDGSVAPARLVDRHFAELPSCIAPGDLLVFNDTKVLKARFFGQKASGGK
IEVLIERVTGTHTALAQIRASKSPGAGTTLRLADAFDVTVGERVEPFFTLHFPAPCLDLIEQHGRLPLPPYIEHDADATD
ETRYQTVYASNPGAVAAPTAGLHFDQPLLDKLDAMGVERATLTLHVGAGTFQPVRVENIAEHKMHSEWYDLPQSLVDKIA
ATRARGGNVIAVGTTSMRALEAAARSADEAGRPLAATQAETDIFITPGYRFRVVDRLVTNFHLPKSTLLMLVSAFAGVET
IRAAYRHAIEERYRFFSYGDAMLLTRRDTPEAPGA
>Mature_355_residues
MFTLSDFDFNLPPELIAQTALPDRTASRLLEVDGSVAPARLVDRHFAELPSCIAPGDLLVFNDTKVLKARFFGQKASGGK
IEVLIERVTGTHTALAQIRASKSPGAGTTLRLADAFDVTVGERVEPFFTLHFPAPCLDLIEQHGRLPLPPYIEHDADATD
ETRYQTVYASNPGAVAAPTAGLHFDQPLLDKLDAMGVERATLTLHVGAGTFQPVRVENIAEHKMHSEWYDLPQSLVDKIA
ATRARGGNVIAVGTTSMRALEAAARSADEAGRPLAATQAETDIFITPGYRFRVVDRLVTNFHLPKSTLLMLVSAFAGVET
IRAAYRHAIEERYRFFSYGDAMLLTRRDTPEAPGA

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=347, Percent_Identity=53.6023054755043, Blast_Score=352, Evalue=2e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38721; Mature: 38721

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTLSDFDFNLPPELIAQTALPDRTASRLLEVDGSVAPARLVDRHFAELPSCIAPGDLLV
CCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHCCCCCEEE
FNDTKVLKARFFGQKASGGKIEVLIERVTGTHTALAQIRASKSPGAGTTLRLADAFDVTV
ECCCHHHHHHHCCCCCCCCEEEEEEEECCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCC
GERVEPFFTLHFPAPCLDLIEQHGRLPLPPYIEHDADATDETRYQTVYASNPGAVAAPTA
CCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEECCCC
GLHFDQPLLDKLDAMGVERATLTLHVGAGTFQPVRVENIAEHKMHSEWYDLPQSLVDKIA
CCCCCCHHHHHHHHCCCCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
ATRARGGNVIAVGTTSMRALEAAARSADEAGRPLAATQAETDIFITPGYRFRVVDRLVTN
HHHCCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEECCCCCEEEECCCCHHHHHHHHHHH
FHLPKSTLLMLVSAFAGVETIRAAYRHAIEERYRFFSYGDAMLLTRRDTPEAPGA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCC
>Mature Secondary Structure
MFTLSDFDFNLPPELIAQTALPDRTASRLLEVDGSVAPARLVDRHFAELPSCIAPGDLLV
CCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHCCCCCEEE
FNDTKVLKARFFGQKASGGKIEVLIERVTGTHTALAQIRASKSPGAGTTLRLADAFDVTV
ECCCHHHHHHHCCCCCCCCEEEEEEEECCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCC
GERVEPFFTLHFPAPCLDLIEQHGRLPLPPYIEHDADATDETRYQTVYASNPGAVAAPTA
CCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEECCCC
GLHFDQPLLDKLDAMGVERATLTLHVGAGTFQPVRVENIAEHKMHSEWYDLPQSLVDKIA
CCCCCCHHHHHHHHCCCCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
ATRARGGNVIAVGTTSMRALEAAARSADEAGRPLAATQAETDIFITPGYRFRVVDRLVTN
HHHCCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEECCCCCEEEECCCCHHHHHHHHHHH
FHLPKSTLLMLVSAFAGVETIRAAYRHAIEERYRFFSYGDAMLLTRRDTPEAPGA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA