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The map label for this gene is tgt [H]

Identifier: 107021798

GI number: 107021798

Start: 253913

End: 255256

Strand: Reverse

Name: tgt [H]

Synonym: Bcen_0238

Alternate gene names: 107021798

Gene position: 255256-253913 (Counterclockwise)

Preceding gene: 107021799

Following gene: 107021797

Centisome position: 7.75

GC content: 65.55

Gene sequence:

>1344_bases
ATGCGCACGATGGCCGGCGCGTCGCACTGCCGCCGTGCCGTTTTTTACGTTGTGCGCGCTACGCTGGCGCGCACCCGCTT
TTCCCCGCCGCCGATTTCCCGGGCGGCGTTTCCAACATGCGCCGGACTGTTTTCCGGTAGCAAGGAGAGCAACACGATGA
CCGAAGGTTCATCCCACGATACGCACGCCCCCGTGCAGGACCGCCCGGCCAACGGGCTCGAATTCGAATTGCTGACGACC
GACGGCCACGCGCGCCGCGGCCGCGTGAAGCTCAATCACGGCACGGTCGAGACACCGATCTTCATGCCGGTCGGCACGTA
CGGCACCGTGAAGGCGATCCAGCCGCGCGAGCTGCACGAGATCAAGGCCCAGATCATCCTCGGCAACACGTTCCACCTGT
GGCTGCGCCCGGGCCTCGACACGATCGACGCGCACGGCGGCCTGCACGGCTTCATGGGCTGGAACAAGCCGATCCTGACC
GATTCGGGTGGCTTCCAGGTGTTCTCGCTCGGCGACCTGCGCAAGATCACCGAGGACGGCGTCACGTTCGCGTCGCCGAT
CAACGGCGACAAGCTGTTCCTGTCGCCGGAAGTGTCGATGCAGATCCAGAAGGTGCTGAACTCCGACGTCGTGATGCAGT
TCGACGAGTGCACGCCGTACGCGACCAACGGCGTGCCGACGACGCACCAGGAAGCGGCCGACTCGATGCGCATGTCGCTG
CGCTGGGCGAAGCGCTCGCTCGACGAATTCCACCGGCTCGGCAACCCGAATGCGCTGTTCGGGATCGTCCAGGGCGGGAT
GTTCGAGGACCTGCGCGACGAATCGCTCGCGGGCCTCGCGGAACTCGGCTTCCACGGCCTCGCGATCGGCGGGCTGTCGG
TGGGCGAGCCGAAGGAAGACATGATGCGCGTGCTGCAGCACGTGGGCCCGAAGCTGCCGGCCAACAAGCCGCACTACCTG
ATGGGCGTGGGCACGCCGGAGGATCTGGTCGAGGGCGTCGCGAACGGCATCGACATGTTCGACTGCGTGATGCCGACCCG
CAACGCGCGCAACGGGTGGCTCTTCACGCGCTTCGGCGACGTGAAGATCCGCAACGCGACGCACAAGAACTCGTTGAAGC
CGCTCGATTCGACCTGCACCTGCTACACGTGCCAGAACTTCTCGCGCGGCTACCTGCATCACCTGCACCGGGTCGGCGAA
ATCCTCGGCGCGCAGCTGAACACGATCCACAACCTGCACTACTACCTGCAGCTGATGAGCGAGATCCGCGACGCGATCGA
AACGCATACGTTCGACGCATTCCGGCAGCGCTTCGCGGAGGATCGCGCCAGAGGGGTCGACTGA

Upstream 100 bases:

>100_bases
AACGCTACCGCTTCTTCAGCTACGGCGACGCGATGCTGCTCACGCGGCGCGACACGCCGGAGGCGCCGGGCGCGTAAGCG
CAGCCGACGGCATCGGCTCG

Downstream 100 bases:

>100_bases
CGGGCCGGCCGGCGGCCCGCACCGCGGCCAGTCACATAGGACGCGTACTAATTGGCACACTCAAATCGCCAGTCGCGACC
GCCAAACATTACAATTACCT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MRTMAGASHCRRAVFYVVRATLARTRFSPPPISRAAFPTCAGLFSGSKESNTMTEGSSHDTHAPVQDRPANGLEFELLTT
DGHARRGRVKLNHGTVETPIFMPVGTYGTVKAIQPRELHEIKAQIILGNTFHLWLRPGLDTIDAHGGLHGFMGWNKPILT
DSGGFQVFSLGDLRKITEDGVTFASPINGDKLFLSPEVSMQIQKVLNSDVVMQFDECTPYATNGVPTTHQEAADSMRMSL
RWAKRSLDEFHRLGNPNALFGIVQGGMFEDLRDESLAGLAELGFHGLAIGGLSVGEPKEDMMRVLQHVGPKLPANKPHYL
MGVGTPEDLVEGVANGIDMFDCVMPTRNARNGWLFTRFGDVKIRNATHKNSLKPLDSTCTCYTCQNFSRGYLHHLHRVGE
ILGAQLNTIHNLHYYLQLMSEIRDAIETHTFDAFRQRFAEDRARGVD

Sequences:

>Translated_447_residues
MRTMAGASHCRRAVFYVVRATLARTRFSPPPISRAAFPTCAGLFSGSKESNTMTEGSSHDTHAPVQDRPANGLEFELLTT
DGHARRGRVKLNHGTVETPIFMPVGTYGTVKAIQPRELHEIKAQIILGNTFHLWLRPGLDTIDAHGGLHGFMGWNKPILT
DSGGFQVFSLGDLRKITEDGVTFASPINGDKLFLSPEVSMQIQKVLNSDVVMQFDECTPYATNGVPTTHQEAADSMRMSL
RWAKRSLDEFHRLGNPNALFGIVQGGMFEDLRDESLAGLAELGFHGLAIGGLSVGEPKEDMMRVLQHVGPKLPANKPHYL
MGVGTPEDLVEGVANGIDMFDCVMPTRNARNGWLFTRFGDVKIRNATHKNSLKPLDSTCTCYTCQNFSRGYLHHLHRVGE
ILGAQLNTIHNLHYYLQLMSEIRDAIETHTFDAFRQRFAEDRARGVD
>Mature_447_residues
MRTMAGASHCRRAVFYVVRATLARTRFSPPPISRAAFPTCAGLFSGSKESNTMTEGSSHDTHAPVQDRPANGLEFELLTT
DGHARRGRVKLNHGTVETPIFMPVGTYGTVKAIQPRELHEIKAQIILGNTFHLWLRPGLDTIDAHGGLHGFMGWNKPILT
DSGGFQVFSLGDLRKITEDGVTFASPINGDKLFLSPEVSMQIQKVLNSDVVMQFDECTPYATNGVPTTHQEAADSMRMSL
RWAKRSLDEFHRLGNPNALFGIVQGGMFEDLRDESLAGLAELGFHGLAIGGLSVGEPKEDMMRVLQHVGPKLPANKPHYL
MGVGTPEDLVEGVANGIDMFDCVMPTRNARNGWLFTRFGDVKIRNATHKNSLKPLDSTCTCYTCQNFSRGYLHHLHRVGE
ILGAQLNTIHNLHYYLQLMSEIRDAIETHTFDAFRQRFAEDRARGVD

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=355, Percent_Identity=44.5070422535211, Blast_Score=296, Evalue=2e-80,
Organism=Escherichia coli, GI1786607, Length=374, Percent_Identity=64.1711229946524, Blast_Score=494, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI17544680, Length=371, Percent_Identity=41.7789757412399, Blast_Score=289, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI25146502, Length=164, Percent_Identity=29.2682926829268, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19920510, Length=355, Percent_Identity=44.5070422535211, Blast_Score=308, Evalue=7e-84,
Organism=Drosophila melanogaster, GI21355311, Length=166, Percent_Identity=29.5180722891566, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 49555; Mature: 49555

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTMAGASHCRRAVFYVVRATLARTRFSPPPISRAAFPTCAGLFSGSKESNTMTEGSSHD
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHHHHHHHCCCCCCCCCCCCCCCC
THAPVQDRPANGLEFELLTTDGHARRGRVKLNHGTVETPIFMPVGTYGTVKAIQPRELHE
CCCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCCCCCEEEECCCCCCEECCCCHHHHH
IKAQIILGNTFHLWLRPGLDTIDAHGGLHGFMGWNKPILTDSGGFQVFSLGDLRKITEDG
HEEEEEECCEEEEEEECCCCCCCCCCCCEECCCCCCCEEECCCCEEEEECCHHHHHHHCC
VTFASPINGDKLFLSPEVSMQIQKVLNSDVVMQFDECTPYATNGVPTTHQEAADSMRMSL
CEEECCCCCCEEEECCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHH
RWAKRSLDEFHRLGNPNALFGIVQGGMFEDLRDESLAGLAELGFHGLAIGGLSVGEPKED
HHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHH
MMRVLQHVGPKLPANKPHYLMGVGTPEDLVEGVANGIDMFDCVMPTRNARNGWLFTRFGD
HHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEEEECC
VKIRNATHKNSLKPLDSTCTCYTCQNFSRGYLHHLHRVGEILGAQLNTIHNLHYYLQLMS
EEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIRDAIETHTFDAFRQRFAEDRARGVD
HHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRTMAGASHCRRAVFYVVRATLARTRFSPPPISRAAFPTCAGLFSGSKESNTMTEGSSHD
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHHHHHHHCCCCCCCCCCCCCCCC
THAPVQDRPANGLEFELLTTDGHARRGRVKLNHGTVETPIFMPVGTYGTVKAIQPRELHE
CCCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCCCCCEEEECCCCCCEECCCCHHHHH
IKAQIILGNTFHLWLRPGLDTIDAHGGLHGFMGWNKPILTDSGGFQVFSLGDLRKITEDG
HEEEEEECCEEEEEEECCCCCCCCCCCCEECCCCCCCEEECCCCEEEEECCHHHHHHHCC
VTFASPINGDKLFLSPEVSMQIQKVLNSDVVMQFDECTPYATNGVPTTHQEAADSMRMSL
CEEECCCCCCEEEECCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHH
RWAKRSLDEFHRLGNPNALFGIVQGGMFEDLRDESLAGLAELGFHGLAIGGLSVGEPKED
HHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHH
MMRVLQHVGPKLPANKPHYLMGVGTPEDLVEGVANGIDMFDCVMPTRNARNGWLFTRFGD
HHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEEEECC
VKIRNATHKNSLKPLDSTCTCYTCQNFSRGYLHHLHRVGEILGAQLNTIHNLHYYLQLMS
EEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIRDAIETHTFDAFRQRFAEDRARGVD
HHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA