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The map label for this gene is speE [H]

Identifier: 107021714

GI number: 107021714

Start: 159641

End: 160498

Strand: Reverse

Name: speE [H]

Synonym: Bcen_0154

Alternate gene names: 107021714

Gene position: 160498-159641 (Counterclockwise)

Preceding gene: 107021717

Following gene: 107021713

Centisome position: 4.87

GC content: 69.93

Gene sequence:

>858_bases
GTGAGCACCACCCTTCTCTTTCACCCGACGCCCGACGCCACCTACGGCTTCCCGAACGCGCGGCGGCTCGCGCACGTCGC
GTCCCCGCACCAGCAGATCGAAGTCTGGGAAACCCCGCAGCTCGGCCGGCTGTTCACGCTGGACGGCCGGCCGATGACGT
CGGTCGGCGACGAATTCGTCTATCACGAGTGCATGACGCACCCGGCCGCGCTCGCGCATCCGGCGCCGAGGAAGGCGCTC
GTGCTCGGCGGCGGCGACGGCGGCGCGGCGCGCCAGCTGCTCAAGCACGCGTGCATTGAACGGATCGTGATCGCCGAACT
CGACGACGAGGTGGTCGGAATGGCGCGCCGCTATCTCGACGACGTGCACCAGGGCGCACTCGACGACCCGCGCGTCGAGG
TCGTGATCGGCGACGCCGCGCACTTCGTCGATTCGACGATCGAACATTTCGATCTTGTCGTGTTCGACCTCACGCCGCCC
GATTCGCCGGCGGCCGGCCTCTATACGCGCGCGTTCTACGCGCGGCTCAAGCGAATCCTCACGCGCTGCGGCGCGATCTC
GATGCATCTCGGCTCGCCGGTGTTCCATGCCGCGCGCATCGCCGCGCTGCTCGACGACCTGCGCGCGAGCTTCGCGGTCG
TCGATCCGCTGTCCGCGCACGTGCCGCTGTACGGCTCGCAGTGGCTGATGGCCATCGCGAGCGACACGCTCGATGCGGCC
GCGTTGTTCGCGCACGACGTCGACGCACGGCTCGCGGCCCGCCGCGTCGACGGCCTGCGCTACTACGATGCGCGGCTGCA
TGCGTCCCTCTTTGCCCTGCCGCGCGCGCTGCGCGATACACTGGGCGTTCGCCGCTGA

Upstream 100 bases:

>100_bases
ATTTCCCTTGTCGGCGCGTAGTGTTCCGGCACGGTTTTCCGCCAAACCGCCTGCCTCCTATAATTCAGGCGTCGGAACTG
CCTGCCCCAGCGGCCCGCTC

Downstream 100 bases:

>100_bases
ACAGCCGGACGGTCGCAACGTTCCGTTGTTCCAGCATGAGACCGGCGCAGGCGCGAGAAGCGCCGACACCGTGCAACAGG
AGATTTTCATGACCGATCCA

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MSTTLLFHPTPDATYGFPNARRLAHVASPHQQIEVWETPQLGRLFTLDGRPMTSVGDEFVYHECMTHPAALAHPAPRKAL
VLGGGDGGAARQLLKHACIERIVIAELDDEVVGMARRYLDDVHQGALDDPRVEVVIGDAAHFVDSTIEHFDLVVFDLTPP
DSPAAGLYTRAFYARLKRILTRCGAISMHLGSPVFHAARIAALLDDLRASFAVVDPLSAHVPLYGSQWLMAIASDTLDAA
ALFAHDVDARLAARRVDGLRYYDARLHASLFALPRALRDTLGVRR

Sequences:

>Translated_285_residues
MSTTLLFHPTPDATYGFPNARRLAHVASPHQQIEVWETPQLGRLFTLDGRPMTSVGDEFVYHECMTHPAALAHPAPRKAL
VLGGGDGGAARQLLKHACIERIVIAELDDEVVGMARRYLDDVHQGALDDPRVEVVIGDAAHFVDSTIEHFDLVVFDLTPP
DSPAAGLYTRAFYARLKRILTRCGAISMHLGSPVFHAARIAALLDDLRASFAVVDPLSAHVPLYGSQWLMAIASDTLDAA
ALFAHDVDARLAARRVDGLRYYDARLHASLFALPRALRDTLGVRR
>Mature_284_residues
STTLLFHPTPDATYGFPNARRLAHVASPHQQIEVWETPQLGRLFTLDGRPMTSVGDEFVYHECMTHPAALAHPAPRKALV
LGGGDGGAARQLLKHACIERIVIAELDDEVVGMARRYLDDVHQGALDDPRVEVVIGDAAHFVDSTIEHFDLVVFDLTPPD
SPAAGLYTRAFYARLKRILTRCGAISMHLGSPVFHAARIAALLDDLRASFAVVDPLSAHVPLYGSQWLMAIASDTLDAAA
LFAHDVDARLAARRVDGLRYYDARLHASLFALPRALRDTLGVRR

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=262, Percent_Identity=27.4809160305344, Blast_Score=119, Evalue=4e-27,
Organism=Escherichia coli, GI1786312, Length=284, Percent_Identity=34.1549295774648, Blast_Score=159, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI25154106, Length=278, Percent_Identity=29.136690647482, Blast_Score=117, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI212645616, Length=216, Percent_Identity=29.6296296296296, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6323175, Length=261, Percent_Identity=28.735632183908, Blast_Score=132, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6325326, Length=257, Percent_Identity=28.4046692607004, Blast_Score=126, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24645443, Length=270, Percent_Identity=29.2592592592593, Blast_Score=131, Evalue=4e-31,
Organism=Drosophila melanogaster, GI21355941, Length=209, Percent_Identity=30.1435406698565, Blast_Score=107, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 31224; Mature: 31093

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTLLFHPTPDATYGFPNARRLAHVASPHQQIEVWETPQLGRLFTLDGRPMTSVGDEFV
CCCEEEEECCCCCCCCCCCHHHHHHHCCCCHHEEEECCCCCCCEEEECCCCCHHHHHHHH
YHECMTHPAALAHPAPRKALVLGGGDGGAARQLLKHACIERIVIAELDDEVVGMARRYLD
HHHHHCCCHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
DVHQGALDDPRVEVVIGDAAHFVDSTIEHFDLVVFDLTPPDSPAAGLYTRAFYARLKRIL
HHHHCCCCCCCEEEEECCHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
TRCGAISMHLGSPVFHAARIAALLDDLRASFAVVDPLSAHVPLYGSQWLMAIASDTLDAA
HHHCCEEECCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHH
ALFAHDVDARLAARRVDGLRYYDARLHASLFALPRALRDTLGVRR
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
STTLLFHPTPDATYGFPNARRLAHVASPHQQIEVWETPQLGRLFTLDGRPMTSVGDEFV
CCEEEEECCCCCCCCCCCHHHHHHHCCCCHHEEEECCCCCCCEEEECCCCCHHHHHHHH
YHECMTHPAALAHPAPRKALVLGGGDGGAARQLLKHACIERIVIAELDDEVVGMARRYLD
HHHHHCCCHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
DVHQGALDDPRVEVVIGDAAHFVDSTIEHFDLVVFDLTPPDSPAAGLYTRAFYARLKRIL
HHHHCCCCCCCEEEEECCHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
TRCGAISMHLGSPVFHAARIAALLDDLRASFAVVDPLSAHVPLYGSQWLMAIASDTLDAA
HHHCCEEECCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHH
ALFAHDVDARLAARRVDGLRYYDARLHASLFALPRALRDTLGVRR
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]