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The map label for this gene is gspO [H]

Identifier: 107021654

GI number: 107021654

Start: 98268

End: 99179

Strand: Reverse

Name: gspO [H]

Synonym: Bcen_0094

Alternate gene names: 107021654

Gene position: 99179-98268 (Counterclockwise)

Preceding gene: 107021655

Following gene: 107021653

Centisome position: 3.01

GC content: 70.07

Gene sequence:

>912_bases
ATGACGCCAGTGCCCCTGTCCACCGTTTCCGATTCGCCCGAGGGCACGCTGCTCGCGCTGGCGATGCTGCCGCCCGCGCT
GCAGTATGCGTTCGCGGTCGTCATCGGCCTTTGCATCGGCAGCTTCCTGAACGTGGTCGTGCACCGGTTGCCCGTGATGA
TGCAGCGCGCGTGGCAGGCCGAGATCGCCGAAGCGACCGGCGCCGCGGGCGCCCCGCCCGACGACGGTTATCCGCCGCGC
TACGATCTGTGGCGCCCGCGCAGCGCCTGCCCGCACTGCGGCCACGTGTTGCGCGCCTGGGAAAACATTCCGCTCCTTAG
CTACCTGATGCTGCGCGGCCGCTGCCGGCGCTGCGGCCATGCAATCGGCATCCGCTATCCGCTCGTCGAACTCGCGTGCG
CACTGCTCGCGGCCGGCTCGCTCGCGGCGTTCGGCCCGACCGTCGCCGCGTTCGCCGCGTTCGCGCTGTGCGCGGCGCTG
CTCGCGATGAGTGCGATCGATATCCGGACCGGCTACCTGCCCGACTCGATGACGCTGCCATTGCTGTGGGCCGGGCTCGT
GCTGAACCTCGGCGGCACTTTCACGTCGCTGCGCTCGGCCGTGATCGGCGCGATGGCCGGCTACCTGTTCCTGTGGTCGA
TCTACTGGCTGTTCAAATGGCTGCGCGGCATCGAGGGCATCGGTTTCGGCGACCTGAAGCTGCTTGCCGCGCTCGGCGCA
TGGATGGGCTGGGCCGCACTGCCGCAGGTCGTGCTGTTCGCGGCGGTGACCGGCGCGGTCGTCGGGCTCGTCGCGACCTG
GCGCGGCCGCATGCGCTTCGAGGAGCCGATTCCATTCGGCCCGTTCCTCGCGGCCGGCGGCGTCGCCACGCTGTTTTTCG
GCACCCCTTTCTATGCGGCGCTCGGAGGCTGA

Upstream 100 bases:

>100_bases
GCGGCCTTGTGATCGCGATGTACCTGCCGATCCTTCAGCTCGGCAACGTGGTGTAGCATCGCAACCGATTCTGTCGCCTT
TTAGTCCAACCTCGAGCCTC

Downstream 100 bases:

>100_bases
CATGTTTTCAGTAGGACTCACGGGCGGCATCGGCAGCGGCAAGACGACCGTTGCCGACCTGTTCGGCGCCCGTGGCGCAT
CGCTCGTCGATACCGACCTG

Product: prepilin peptidase

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MTPVPLSTVSDSPEGTLLALAMLPPALQYAFAVVIGLCIGSFLNVVVHRLPVMMQRAWQAEIAEATGAAGAPPDDGYPPR
YDLWRPRSACPHCGHVLRAWENIPLLSYLMLRGRCRRCGHAIGIRYPLVELACALLAAGSLAAFGPTVAAFAAFALCAAL
LAMSAIDIRTGYLPDSMTLPLLWAGLVLNLGGTFTSLRSAVIGAMAGYLFLWSIYWLFKWLRGIEGIGFGDLKLLAALGA
WMGWAALPQVVLFAAVTGAVVGLVATWRGRMRFEEPIPFGPFLAAGGVATLFFGTPFYAALGG

Sequences:

>Translated_303_residues
MTPVPLSTVSDSPEGTLLALAMLPPALQYAFAVVIGLCIGSFLNVVVHRLPVMMQRAWQAEIAEATGAAGAPPDDGYPPR
YDLWRPRSACPHCGHVLRAWENIPLLSYLMLRGRCRRCGHAIGIRYPLVELACALLAAGSLAAFGPTVAAFAAFALCAAL
LAMSAIDIRTGYLPDSMTLPLLWAGLVLNLGGTFTSLRSAVIGAMAGYLFLWSIYWLFKWLRGIEGIGFGDLKLLAALGA
WMGWAALPQVVLFAAVTGAVVGLVATWRGRMRFEEPIPFGPFLAAGGVATLFFGTPFYAALGG
>Mature_302_residues
TPVPLSTVSDSPEGTLLALAMLPPALQYAFAVVIGLCIGSFLNVVVHRLPVMMQRAWQAEIAEATGAAGAPPDDGYPPRY
DLWRPRSACPHCGHVLRAWENIPLLSYLMLRGRCRRCGHAIGIRYPLVELACALLAAGSLAAFGPTVAAFAAFALCAALL
AMSAIDIRTGYLPDSMTLPLLWAGLVLNLGGTFTSLRSAVIGAMAGYLFLWSIYWLFKWLRGIEGIGFGDLKLLAALGAW
MGWAALPQVVLFAAVTGAVVGLVATWRGRMRFEEPIPFGPFLAAGGVATLFFGTPFYAALGG

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=274, Percent_Identity=37.5912408759124, Blast_Score=161, Evalue=6e-41,
Organism=Escherichia coli, GI1789732, Length=148, Percent_Identity=45.2702702702703, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 32228; Mature: 32096

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPVPLSTVSDSPEGTLLALAMLPPALQYAFAVVIGLCIGSFLNVVVHRLPVMMQRAWQA
CCCCCCCCCCCCCCCCEEHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIAEATGAAGAPPDDGYPPRYDLWRPRSACPHCGHVLRAWENIPLLSYLMLRGRCRRCGH
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
AIGIRYPLVELACALLAAGSLAAFGPTVAAFAAFALCAALLAMSAIDIRTGYLPDSMTLP
HHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
LLWAGLVLNLGGTFTSLRSAVIGAMAGYLFLWSIYWLFKWLRGIEGIGFGDLKLLAALGA
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
WMGWAALPQVVLFAAVTGAVVGLVATWRGRMRFEEPIPFGPFLAAGGVATLFFGTPFYAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCHHHHHHCCHHHHH
LGG
CCC
>Mature Secondary Structure 
TPVPLSTVSDSPEGTLLALAMLPPALQYAFAVVIGLCIGSFLNVVVHRLPVMMQRAWQA
CCCCCCCCCCCCCCCEEHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIAEATGAAGAPPDDGYPPRYDLWRPRSACPHCGHVLRAWENIPLLSYLMLRGRCRRCGH
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
AIGIRYPLVELACALLAAGSLAAFGPTVAAFAAFALCAALLAMSAIDIRTGYLPDSMTLP
HHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
LLWAGLVLNLGGTFTSLRSAVIGAMAGYLFLWSIYWLFKWLRGIEGIGFGDLKLLAALGA
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
WMGWAALPQVVLFAAVTGAVVGLVATWRGRMRFEEPIPFGPFLAAGGVATLFFGTPFYAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCHHHHHHCCHHHHH
LGG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10419967 [H]