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The map label for this gene is ubiE [H]

Identifier: 103488661

GI number: 103488661

Start: 3342744

End: 3343511

Strand: Reverse

Name: ubiE [H]

Synonym: Sala_3186

Alternate gene names: 103488661

Gene position: 3343511-3342744 (Counterclockwise)

Preceding gene: NA

Following gene: 103488660

Centisome position: 99.95

GC content: 63.28

Gene sequence:

>768_bases
ATGACCTCCCCCGTAAAAGGCGCGCAAACGATGGCCACTCCCGATACCGTCAGCTTTGGCTATGAGCAGGTTCCCGCGGG
CGCGAAGACCGCGATGGTCGGCGAGGTGTTCAGCCGTGTCGCGCGCAAATATGACATCATGAATGACGCGATGTCGGGCG
GGATGCACCGATTGTGGAAGGACCGTTTCGTGCGCCGCGTCAAGCCGCGCGCGGGCGAGGCGATCCTCGACATGGCGGGC
GGCACGGGCGACATCGCCTTTCGCATGGAGCCGTCGGGGGCGAACATCACGGTCGCGGACATCAACCCCGACATGCTGGG
CGTGGGAATGGAACGGGCGGCGGCGCGCGGTATCGACAGTCTGGTCTGGTCCGAACAGAATGCCGAAAAGCTGACATTCC
CGGATCGCTTTTTCGACGCCTATACGATCGCTTTCGGCATCCGCAACGTCACCGACATTCCGGCGGCGCTGAGCGAAGCG
CACCGCGTGCTGCGCTTTGGCGGGCGCTTTTTCTGCCTCGAGTTTTCGACCAACCAATGGCCGGGGTTCGCCGAGGCCTA
TGACGCCTATTCGCACCATCTGGTGCCGAAGCTCGGCAAGCTGATCGCGGGTGACGAGGAAAGCTATCGTTACCTCATCG
AATCGATCCGCCGCTTTCCGCCGATGCCCGAATTCGCCCGGATGATCGGCGAGGCGGGTTTCACCGCGGTCAAAGTCGAA
CCGATCCTCGGCGGACTCGTCGCGATCCACAGCGGGTGGAAGGCGTGA

Upstream 100 bases:

>100_bases
CGCACGGTGGTTTCGACTTCGGGAAGTTCGGGCATCAGGCTTGCCCCGCTACGCGCCATTTGCTTGCGCGGCAAGGCTTG
GCTAAAGGCCGACGCACCAG

Downstream 100 bases:

>100_bases
GCCGGCACCGTCCCGCGAGCGCCCCTGCGAAGGCAGGGGCCGATCTCCTTGGGACCGGTCATCGCCCCGGCGCCCGCTGG
CTTCCCCCGTTCGCGGGCGC

Product: ubiquinone/menaquinone biosynthesis methyltransferases

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MTSPVKGAQTMATPDTVSFGYEQVPAGAKTAMVGEVFSRVARKYDIMNDAMSGGMHRLWKDRFVRRVKPRAGEAILDMAG
GTGDIAFRMEPSGANITVADINPDMLGVGMERAAARGIDSLVWSEQNAEKLTFPDRFFDAYTIAFGIRNVTDIPAALSEA
HRVLRFGGRFFCLEFSTNQWPGFAEAYDAYSHHLVPKLGKLIAGDEESYRYLIESIRRFPPMPEFARMIGEAGFTAVKVE
PILGGLVAIHSGWKA

Sequences:

>Translated_255_residues
MTSPVKGAQTMATPDTVSFGYEQVPAGAKTAMVGEVFSRVARKYDIMNDAMSGGMHRLWKDRFVRRVKPRAGEAILDMAG
GTGDIAFRMEPSGANITVADINPDMLGVGMERAAARGIDSLVWSEQNAEKLTFPDRFFDAYTIAFGIRNVTDIPAALSEA
HRVLRFGGRFFCLEFSTNQWPGFAEAYDAYSHHLVPKLGKLIAGDEESYRYLIESIRRFPPMPEFARMIGEAGFTAVKVE
PILGGLVAIHSGWKA
>Mature_254_residues
TSPVKGAQTMATPDTVSFGYEQVPAGAKTAMVGEVFSRVARKYDIMNDAMSGGMHRLWKDRFVRRVKPRAGEAILDMAGG
TGDIAFRMEPSGANITVADINPDMLGVGMERAAARGIDSLVWSEQNAEKLTFPDRFFDAYTIAFGIRNVTDIPAALSEAH
RVLRFGGRFFCLEFSTNQWPGFAEAYDAYSHHLVPKLGKLIAGDEESYRYLIESIRRFPPMPEFARMIGEAGFTAVKVEP
ILGGLVAIHSGWKA

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) [H]

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Homo sapiens, GI116063536, Length=273, Percent_Identity=47.6190476190476, Blast_Score=236, Evalue=1e-62,
Organism=Escherichia coli, GI48994983, Length=246, Percent_Identity=44.3089430894309, Blast_Score=208, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI17557039, Length=241, Percent_Identity=46.4730290456432, Blast_Score=204, Evalue=5e-53,
Organism=Saccharomyces cerevisiae, GI6323526, Length=276, Percent_Identity=39.1304347826087, Blast_Score=191, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24641702, Length=271, Percent_Identity=47.9704797047971, Blast_Score=245, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28048; Mature: 27917

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS01183 UBIE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPVKGAQTMATPDTVSFGYEQVPAGAKTAMVGEVFSRVARKYDIMNDAMSGGMHRLWK
CCCCCCCCCCCCCCCCHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRFVRRVKPRAGEAILDMAGGTGDIAFRMEPSGANITVADINPDMLGVGMERAAARGIDS
HHHHHHHCCCCCCCHHCCCCCCCCEEEEECCCCCEEEEEECCCCHHHCCHHHHHHHCHHH
LVWSEQNAEKLTFPDRFFDAYTIAFGIRNVTDIPAALSEAHRVLRFGGRFFCLEFSTNQW
HHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCC
PGFAEAYDAYSHHLVPKLGKLIAGDEESYRYLIESIRRFPPMPEFARMIGEAGFTAVKVE
CCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEH
PILGGLVAIHSGWKA
HHHHHHHHHHCCCCC
>Mature Secondary Structure 
TSPVKGAQTMATPDTVSFGYEQVPAGAKTAMVGEVFSRVARKYDIMNDAMSGGMHRLWK
CCCCCCCCCCCCCCCHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRFVRRVKPRAGEAILDMAGGTGDIAFRMEPSGANITVADINPDMLGVGMERAAARGIDS
HHHHHHHCCCCCCCHHCCCCCCCCEEEEECCCCCEEEEEECCCCHHHCCHHHHHHHCHHH
LVWSEQNAEKLTFPDRFFDAYTIAFGIRNVTDIPAALSEAHRVLRFGGRFFCLEFSTNQW
HHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCC
PGFAEAYDAYSHHLVPKLGKLIAGDEESYRYLIESIRRFPPMPEFARMIGEAGFTAVKVE
CCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEH
PILGGLVAIHSGWKA
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA