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The map label for this gene is mrcA [H]

Identifier: 103488651

GI number: 103488651

Start: 3333073

End: 3335589

Strand: Direct

Name: mrcA [H]

Synonym: Sala_3176

Alternate gene names: 103488651

Gene position: 3333073-3335589 (Clockwise)

Preceding gene: 103488650

Following gene: 103488652

Centisome position: 99.64

GC content: 65.87

Gene sequence:

>2517_bases
ATGGCCGCCGACCGTTCGACCGATTTCCGCCTGCGGCTGCGCCGCGACAGCAACGCCATCATCGCCTGGGTCCGCGAGAT
GTGGACGCGGCGCTGGTTCCGCTGGCTGGGCTATCTTGCGCTCGCGGGCCTCGCCGGTATCGCGCTCATCTGGGCGATCT
TCGCGCGCGACCTGCCCTCGGTCGACCAGCTGCGCACCTATGAACCGCCGCTGCCGACCATGGTGCGCGACGGTGAAGGC
AAGCCGGTGCACAGCTATGCGCGCGAGCGCCGCGTCCAGCTCGACTATAGCGAATATCCGCCGCTGCTCGTGCGCGCTTT
CCTTGCGGCGGAGGACAAAACCTTCTTCAGCCACGGCGGCATCGACTATCCCGGCATCGTGTCGGCGGTCATCACCAACC
TGTCGAACAGCGGCCGTCCGGTCGGCGCGTCGACGATCACCCAGCAGGTCGCGAAGAACCTGCTGCTCACCAACGAGCTG
AGCTATCGCCGCAAGATTCGCGAGGCGATTCTCGCGATGCGGATCGAGGATGCGCTGACCAAGGAACAGATCCTCGAACT
CTATCTCAACGAAATCCCCCTCGGGCGACGCAGCTTCGGCGTCCAGGCGGCGAGCCGCGCCTATTTCGACAAGGATGTCG
ACGAGTTGGCGCTGCACGAAATGGCCTTCCTCGCCATTCTGCCCAAGGCGCCCGAACGCTATGGCCGCGCGCGTTTCGAG
CGCGACGCGATCGAGCGGCGCAACTTCGTGCTCGGTGCGATGGAGGACAATGGCTGGATCACCACGGCGCAGCGCGACGC
CGCGCGCGCCATGCCGCTCGGCCTCACGACCAGCGGCAACCGAGCCGTGGCCCAGGTCGGCGGCTATTATATGGAAGAGG
TGCGGCGCCAGCTGATCGCCGAGTTCGGCGAAACCGCCGAGGACGGCCCGCACAGCGTCTATGCTGGCGGCCTCTGGGTG
CGCACGCCCTATGACGGCAAGATGCAGGCGGCGGCGACGCGCGCGCTGCGCAAGGGATTGCAACGCTATGATGCGGGCAA
GGGCTGGTCAGGGCCGATCGCGACGATCGAGGCCGACGACCAGTGGCAGAGCCGCCTCGCGTCGAGTTTCATCGGCATCG
ACTATGACAATTGGCGCATTGCCGCGGTGCTGTCGAAAAGCGCGGGCGCGGCGCGCATCGGCTTCGCCGATGGCGACACC
GGCACGCTTCCTGCCAGCGCCGCGACGATGGGCTATCGCAAGACCGGCGGCAGCGCCTTTTCGGCGCTCCGTCCGGGCGA
TCTCATCGCGGTCAAATCGACCGGCGGCAGCAGCTACGCCCTGCGGAACATTCCCGAAGTGTCGGGCGGCATGGTCGTCG
AAAGTCCGCATTCGGGGCGCATCTATGCGATGCAGGGCGGTTTCGACGTCCGCCTGTCGCCCTTCAACCGCGCGACCCAG
GCCGAGCGCCAGCCGGGATCGACGATAAAGCCCTTCGTTTACGCCGCCGCGCTCGACAATGGCATGACCCCCGCGACGAT
GATCGTCGACGGGCCCTTCTGCGTTTATCAGGGCGCGGCGCTGGGCAACAAATGTTTCCGCAACTTCGGCAATTCGGGAG
GCAGCGGCGAACATACGATGCGCTGGGGGCTCGAACAGTCGCGCAACCTGATGACGGTGCGCGCGGCGAGCCAGATCGGC
ATGGAGCCGGTTGTCGAAATGATCGCCCGGATGGGCATCGGCCAGCACGAACCCTATCTGTCGACCGCGCTCGGTGCCGG
GTCCACGACGGTCGAAAAGATGACCAACGCCTATGCCATGCTTGCAAACCATGGACGCGAACTCAAACCGCGGGTGATAG
ACTATGCGCAGGATCGCCGCGGCAAGGTGATCTTTCCCCGGAACTGGAAACCGTGCGACGGCTGCAACATGAAGGATTGG
GACGGCCGCCCGATGCCGCGCTTCGCGCGCTCGGGACGGCAGCTGATGGACCCGATGACCGCCTATCAGGTCGTCCACAT
GCTCGAAGGCGTCGTCCAGCGCGGCACAGCAGTGCGGCTGCGCGACCTTGGCGTTCCCCTATTCGGCAAGACCGGCACAA
CCTCGGGACCGAATGATGTCTGGTTCGTCGGGGGCACGCCCGATGTCGTCGCGGGCATGTATATCGGCTTTGACCAGCCG
CGCAGCATGGGTGGTTATGCGCAGGGCAGCAGCTATGCGGCGCCGATTTTCAAGGATTTCGCGCTCGAGGCGCTCGCCGA
TCGTCAGCCGATTCCCTTTGCAGCGCCCAAGGGGATACGAATGGTCCGCATCGATCGCCGCTCGGGGCGCCGCGTCTATG
GCAGCTGGCCGGGGACCGATCCCCTGGCGGCAATTATCTGGGAAGCCTTCAAGCCTGAAAGCGAGCCGCGGCGGACGATC
CGCGAGGACGAAATCAGGCCTCCCAAACCGCCGCAGCGGCAGGACGCGCCGGTTCAGCAAACGCCGGGGCGTCGGAGCGA
CAGCGAGTTTCTGGAGGATCGCGGCGGCATTATCTGA

Upstream 100 bases:

>100_bases
ATCTGGCCGGTCAGCGCCCGCGGCTGTGCGCGATGATCCCGGCGCGCTCCGCCGCAAGCACGCTTCCCTTTCGGCGATCC
ACGCTTTAGAGGCGGGACAT

Downstream 100 bases:

>100_bases
CGCCGCGCGCCTTCGTTCGACCCGCCTTTCCTTGAAGGCCGCGAAGCCCTAGGGTCAACATTTTTCAGGAGCAACGACAT
GCGCGCCGAAGCGCTGGATC

Product: glycosyl transferase family protein

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 838; Mature: 837

Protein sequence:

>838_residues
MAADRSTDFRLRLRRDSNAIIAWVREMWTRRWFRWLGYLALAGLAGIALIWAIFARDLPSVDQLRTYEPPLPTMVRDGEG
KPVHSYARERRVQLDYSEYPPLLVRAFLAAEDKTFFSHGGIDYPGIVSAVITNLSNSGRPVGASTITQQVAKNLLLTNEL
SYRRKIREAILAMRIEDALTKEQILELYLNEIPLGRRSFGVQAASRAYFDKDVDELALHEMAFLAILPKAPERYGRARFE
RDAIERRNFVLGAMEDNGWITTAQRDAARAMPLGLTTSGNRAVAQVGGYYMEEVRRQLIAEFGETAEDGPHSVYAGGLWV
RTPYDGKMQAAATRALRKGLQRYDAGKGWSGPIATIEADDQWQSRLASSFIGIDYDNWRIAAVLSKSAGAARIGFADGDT
GTLPASAATMGYRKTGGSAFSALRPGDLIAVKSTGGSSYALRNIPEVSGGMVVESPHSGRIYAMQGGFDVRLSPFNRATQ
AERQPGSTIKPFVYAAALDNGMTPATMIVDGPFCVYQGAALGNKCFRNFGNSGGSGEHTMRWGLEQSRNLMTVRAASQIG
MEPVVEMIARMGIGQHEPYLSTALGAGSTTVEKMTNAYAMLANHGRELKPRVIDYAQDRRGKVIFPRNWKPCDGCNMKDW
DGRPMPRFARSGRQLMDPMTAYQVVHMLEGVVQRGTAVRLRDLGVPLFGKTGTTSGPNDVWFVGGTPDVVAGMYIGFDQP
RSMGGYAQGSSYAAPIFKDFALEALADRQPIPFAAPKGIRMVRIDRRSGRRVYGSWPGTDPLAAIIWEAFKPESEPRRTI
REDEIRPPKPPQRQDAPVQQTPGRRSDSEFLEDRGGII

Sequences:

>Translated_838_residues
MAADRSTDFRLRLRRDSNAIIAWVREMWTRRWFRWLGYLALAGLAGIALIWAIFARDLPSVDQLRTYEPPLPTMVRDGEG
KPVHSYARERRVQLDYSEYPPLLVRAFLAAEDKTFFSHGGIDYPGIVSAVITNLSNSGRPVGASTITQQVAKNLLLTNEL
SYRRKIREAILAMRIEDALTKEQILELYLNEIPLGRRSFGVQAASRAYFDKDVDELALHEMAFLAILPKAPERYGRARFE
RDAIERRNFVLGAMEDNGWITTAQRDAARAMPLGLTTSGNRAVAQVGGYYMEEVRRQLIAEFGETAEDGPHSVYAGGLWV
RTPYDGKMQAAATRALRKGLQRYDAGKGWSGPIATIEADDQWQSRLASSFIGIDYDNWRIAAVLSKSAGAARIGFADGDT
GTLPASAATMGYRKTGGSAFSALRPGDLIAVKSTGGSSYALRNIPEVSGGMVVESPHSGRIYAMQGGFDVRLSPFNRATQ
AERQPGSTIKPFVYAAALDNGMTPATMIVDGPFCVYQGAALGNKCFRNFGNSGGSGEHTMRWGLEQSRNLMTVRAASQIG
MEPVVEMIARMGIGQHEPYLSTALGAGSTTVEKMTNAYAMLANHGRELKPRVIDYAQDRRGKVIFPRNWKPCDGCNMKDW
DGRPMPRFARSGRQLMDPMTAYQVVHMLEGVVQRGTAVRLRDLGVPLFGKTGTTSGPNDVWFVGGTPDVVAGMYIGFDQP
RSMGGYAQGSSYAAPIFKDFALEALADRQPIPFAAPKGIRMVRIDRRSGRRVYGSWPGTDPLAAIIWEAFKPESEPRRTI
REDEIRPPKPPQRQDAPVQQTPGRRSDSEFLEDRGGII
>Mature_837_residues
AADRSTDFRLRLRRDSNAIIAWVREMWTRRWFRWLGYLALAGLAGIALIWAIFARDLPSVDQLRTYEPPLPTMVRDGEGK
PVHSYARERRVQLDYSEYPPLLVRAFLAAEDKTFFSHGGIDYPGIVSAVITNLSNSGRPVGASTITQQVAKNLLLTNELS
YRRKIREAILAMRIEDALTKEQILELYLNEIPLGRRSFGVQAASRAYFDKDVDELALHEMAFLAILPKAPERYGRARFER
DAIERRNFVLGAMEDNGWITTAQRDAARAMPLGLTTSGNRAVAQVGGYYMEEVRRQLIAEFGETAEDGPHSVYAGGLWVR
TPYDGKMQAAATRALRKGLQRYDAGKGWSGPIATIEADDQWQSRLASSFIGIDYDNWRIAAVLSKSAGAARIGFADGDTG
TLPASAATMGYRKTGGSAFSALRPGDLIAVKSTGGSSYALRNIPEVSGGMVVESPHSGRIYAMQGGFDVRLSPFNRATQA
ERQPGSTIKPFVYAAALDNGMTPATMIVDGPFCVYQGAALGNKCFRNFGNSGGSGEHTMRWGLEQSRNLMTVRAASQIGM
EPVVEMIARMGIGQHEPYLSTALGAGSTTVEKMTNAYAMLANHGRELKPRVIDYAQDRRGKVIFPRNWKPCDGCNMKDWD
GRPMPRFARSGRQLMDPMTAYQVVHMLEGVVQRGTAVRLRDLGVPLFGKTGTTSGPNDVWFVGGTPDVVAGMYIGFDQPR
SMGGYAQGSSYAAPIFKDFALEALADRQPIPFAAPKGIRMVRIDRRSGRRVYGSWPGTDPLAAIIWEAFKPESEPRRTIR
EDEIRPPKPPQRQDAPVQQTPGRRSDSEFLEDRGGII

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=813, Percent_Identity=32.2263222632226, Blast_Score=338, Evalue=8e-94,
Organism=Escherichia coli, GI1786343, Length=278, Percent_Identity=36.3309352517986, Blast_Score=131, Evalue=2e-31,
Organism=Escherichia coli, GI1789601, Length=161, Percent_Identity=39.1304347826087, Blast_Score=94, Evalue=4e-20,
Organism=Escherichia coli, GI1788867, Length=201, Percent_Identity=34.3283582089552, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 92494; Mature: 92363

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAADRSTDFRLRLRRDSNAIIAWVREMWTRRWFRWLGYLALAGLAGIALIWAIFARDLPS
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VDQLRTYEPPLPTMVRDGEGKPVHSYARERRVQLDYSEYPPLLVRAFLAAEDKTFFSHGG
HHHHCCCCCCCCCCEECCCCCCHHHHHHHCCEEECHHHCCHHHHHHHHHHCCCCHHHCCC
IDYPGIVSAVITNLSNSGRPVGASTITQQVAKNLLLTNELSYRRKIREAILAMRIEDALT
CCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEQILELYLNEIPLGRRSFGVQAASRAYFDKDVDELALHEMAFLAILPKAPERYGRARFE
HHHHHHHHHHHCCCCCHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
RDAIERRNFVLGAMEDNGWITTAQRDAARAMPLGLTTSGNRAVAQVGGYYMEEVRRQLIA
HHHHHHCCEEEEEEECCCEEEECCHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHH
EFGETAEDGPHSVYAGGLWVRTPYDGKMQAAATRALRKGLQRYDAGKGWSGPIATIEADD
HHCCCCCCCCCEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCH
QWQSRLASSFIGIDYDNWRIAAVLSKSAGAARIGFADGDTGTLPASAATMGYRKTGGSAF
HHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEECCCCCCCCCCHHHHCCCCCCCCCHH
SALRPGDLIAVKSTGGSSYALRNIPEVSGGMVVESPHSGRIYAMQGGFDVRLSPFNRATQ
HCCCCCCEEEEECCCCCCEEECCCCCCCCCEEEECCCCCEEEEEECCEEEEECCCCHHHH
AERQPGSTIKPFVYAAALDNGMTPATMIVDGPFCVYQGAALGNKCFRNFGNSGGSGEHTM
HHCCCCCCCCHHHEEEHHCCCCCCEEEEECCCHHEECCCHHHHHHHHHCCCCCCCCCHHH
RWGLEQSRNLMTVRAASQIGMEPVVEMIARMGIGQHEPYLSTALGAGSTTVEKMTNAYAM
HHCHHHCCCEEEEEHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHH
LANHGRELKPRVIDYAQDRRGKVIFPRNWKPCDGCNMKDWDGRPMPRFARSGRQLMDPMT
HHHCCCCCCCHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHH
AYQVVHMLEGVVQRGTAVRLRDLGVPLFGKTGTTSGPNDVWFVGGTPDVVAGMYIGFDQP
HHHHHHHHHHHHHCCCEEEEHHCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHCCCCCC
RSMGGYAQGSSYAAPIFKDFALEALADRQPIPFAAPKGIRMVRIDRRSGRRVYGSWPGTD
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCC
PLAAIIWEAFKPESEPRRTIREDEIRPPKPPQRQDAPVQQTPGRRSDSEFLEDRGGII
HHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCC
>Mature Secondary Structure 
AADRSTDFRLRLRRDSNAIIAWVREMWTRRWFRWLGYLALAGLAGIALIWAIFARDLPS
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VDQLRTYEPPLPTMVRDGEGKPVHSYARERRVQLDYSEYPPLLVRAFLAAEDKTFFSHGG
HHHHCCCCCCCCCCEECCCCCCHHHHHHHCCEEECHHHCCHHHHHHHHHHCCCCHHHCCC
IDYPGIVSAVITNLSNSGRPVGASTITQQVAKNLLLTNELSYRRKIREAILAMRIEDALT
CCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEQILELYLNEIPLGRRSFGVQAASRAYFDKDVDELALHEMAFLAILPKAPERYGRARFE
HHHHHHHHHHHCCCCCHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
RDAIERRNFVLGAMEDNGWITTAQRDAARAMPLGLTTSGNRAVAQVGGYYMEEVRRQLIA
HHHHHHCCEEEEEEECCCEEEECCHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHH
EFGETAEDGPHSVYAGGLWVRTPYDGKMQAAATRALRKGLQRYDAGKGWSGPIATIEADD
HHCCCCCCCCCEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCH
QWQSRLASSFIGIDYDNWRIAAVLSKSAGAARIGFADGDTGTLPASAATMGYRKTGGSAF
HHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEECCCCCCCCCCHHHHCCCCCCCCCHH
SALRPGDLIAVKSTGGSSYALRNIPEVSGGMVVESPHSGRIYAMQGGFDVRLSPFNRATQ
HCCCCCCEEEEECCCCCCEEECCCCCCCCCEEEECCCCCEEEEEECCEEEEECCCCHHHH
AERQPGSTIKPFVYAAALDNGMTPATMIVDGPFCVYQGAALGNKCFRNFGNSGGSGEHTM
HHCCCCCCCCHHHEEEHHCCCCCCEEEEECCCHHEECCCHHHHHHHHHCCCCCCCCCHHH
RWGLEQSRNLMTVRAASQIGMEPVVEMIARMGIGQHEPYLSTALGAGSTTVEKMTNAYAM
HHCHHHCCCEEEEEHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHH
LANHGRELKPRVIDYAQDRRGKVIFPRNWKPCDGCNMKDWDGRPMPRFARSGRQLMDPMT
HHHCCCCCCCHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHH
AYQVVHMLEGVVQRGTAVRLRDLGVPLFGKTGTTSGPNDVWFVGGTPDVVAGMYIGFDQP
HHHHHHHHHHHHHCCCEEEEHHCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHCCCCCC
RSMGGYAQGSSYAAPIFKDFALEALADRQPIPFAAPKGIRMVRIDRRSGRRVYGSWPGTD
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCC
PLAAIIWEAFKPESEPRRTIREDEIRPPKPPQRQDAPVQQTPGRRSDSEFLEDRGGII
HHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA