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The map label for this gene is prfB [H]

Identifier: 103488652

GI number: 103488652

Start: 3335668

End: 3336795

Strand: Direct

Name: prfB [H]

Synonym: Sala_3177

Alternate gene names: 103488652

Gene position: 3335668-3336795 (Clockwise)

Preceding gene: 103488651

Following gene: 103488653

Centisome position: 99.72

GC content: 64.27

Gene sequence:

>1128_bases
ATGCGCGCCGAAGCGCTGGATCACATCGACAAGATTACCGCCGCTCTCGCGTTGCTGCGCCGCTTTCTCGACTGGGACCG
CGCGGTGCGGCGCCTCGACGAGCTCAATGCCAAGGTCGAAGACCCGACGCTGTGGGACAATCCGAAGGCCGCCCAGGACG
TGATGCGCGAACGGCGTCGACTCGACGAGGCGATCACCGCGACGCGCGCGATCGAGCGCGAATGCGCCGACACCGCCGAG
CTGATCGAGCTGGCCGAGATGGAAGGTGACGAGGCGATGGTCGACGAGGCGGTCGCCAGCCTCGCCGCGCTCGCCGCGCG
CGCCGAAGAGGATAAGGTAAAGGCGCTGCTCGCCGGCGAGGCCGACGGCAACGACGCCTATATCGAAGTCCACGCGGGCG
CGGGCGGCACCGAAAGCCAGGATTGGGCCGAAATGCTCCAGCGCATGTATATGCGCTGGGCCGAAAAGCGCGGAATGAAG
GTCGAGTTGATCGAATATCAGGCGGGCGAACAGGCGGGCATCAAGTCGGCGACGATGCTGGTGAAGGGCGAGAATGCCTA
TGGCTATGCCAAGACCGAAAGCGGCGTCCACCGTCTTGTCCGCATCTCGCCCTATGACAGCTCGGCGCGCCGTCACACCA
GCTTTTCCAGCGTGTGGGTCTATCCGGTGATCGACGATAATATCGAGATCGAGATCAACGAGGGCGACCTCAAGATCGAC
ACCTATCGTGCCTCGGGTGCGGGCGGCCAGCATGTCAACACGACCGATTCGGCGGTGCGCATCACGCACATCCCGACGGG
CATCGTCGTCGCCAGCCAGAATGATCGTTCGCAGCACAAGAACCGCGCGACCGCGATGGGAATGCTGAAAGCGCGGCTCT
ATGAGGCCGAACTGCAAAAGCGCGAGGCCGCGGCATCGGGCGAATATCAGGCGAAGACCGAGATCGGATGGGGCCACCAG
ATCCGTTCCTATGTGCTCCAGCCCTATCAGCTGGTGAAGGATCTGCGCACCGGCGTCACCTCGACCGCGCCGGGCGACGT
TCTCGATGGCGCCCTCGATCCCTTCATGGCGGCGGCGCTGTCGCAGAAGGTGACGGGCGAAAAGGTCGAGGTGGAGGACA
TCGACTGA

Upstream 100 bases:

>100_bases
GGATCGCGGCGGCATTATCTGACGCCGCGCGCCTTCGTTCGACCCGCCTTTCCTTGAAGGCCGCGAAGCCCTAGGGTCAA
CATTTTTCAGGAGCAACGAC

Downstream 100 bases:

>100_bases
TGGTGCGCGGCGCCCTCGCTGCGCTGGCGCTGCTGGCGCTGCTCGGTGGCTGCGACGGCCCGTGGAGCGAGGATCGCAGC
CGCATCGAAACCGCGCGCGA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MRAEALDHIDKITAALALLRRFLDWDRAVRRLDELNAKVEDPTLWDNPKAAQDVMRERRRLDEAITATRAIERECADTAE
LIELAEMEGDEAMVDEAVASLAALAARAEEDKVKALLAGEADGNDAYIEVHAGAGGTESQDWAEMLQRMYMRWAEKRGMK
VELIEYQAGEQAGIKSATMLVKGENAYGYAKTESGVHRLVRISPYDSSARRHTSFSSVWVYPVIDDNIEIEINEGDLKID
TYRASGAGGQHVNTTDSAVRITHIPTGIVVASQNDRSQHKNRATAMGMLKARLYEAELQKREAAASGEYQAKTEIGWGHQ
IRSYVLQPYQLVKDLRTGVTSTAPGDVLDGALDPFMAAALSQKVTGEKVEVEDID

Sequences:

>Translated_375_residues
MRAEALDHIDKITAALALLRRFLDWDRAVRRLDELNAKVEDPTLWDNPKAAQDVMRERRRLDEAITATRAIERECADTAE
LIELAEMEGDEAMVDEAVASLAALAARAEEDKVKALLAGEADGNDAYIEVHAGAGGTESQDWAEMLQRMYMRWAEKRGMK
VELIEYQAGEQAGIKSATMLVKGENAYGYAKTESGVHRLVRISPYDSSARRHTSFSSVWVYPVIDDNIEIEINEGDLKID
TYRASGAGGQHVNTTDSAVRITHIPTGIVVASQNDRSQHKNRATAMGMLKARLYEAELQKREAAASGEYQAKTEIGWGHQ
IRSYVLQPYQLVKDLRTGVTSTAPGDVLDGALDPFMAAALSQKVTGEKVEVEDID
>Mature_375_residues
MRAEALDHIDKITAALALLRRFLDWDRAVRRLDELNAKVEDPTLWDNPKAAQDVMRERRRLDEAITATRAIERECADTAE
LIELAEMEGDEAMVDEAVASLAALAARAEEDKVKALLAGEADGNDAYIEVHAGAGGTESQDWAEMLQRMYMRWAEKRGMK
VELIEYQAGEQAGIKSATMLVKGENAYGYAKTESGVHRLVRISPYDSSARRHTSFSSVWVYPVIDDNIEIEINEGDLKID
TYRASGAGGQHVNTTDSAVRITHIPTGIVVASQNDRSQHKNRATAMGMLKARLYEAELQKREAAASGEYQAKTEIGWGHQ
IRSYVLQPYQLVKDLRTGVTSTAPGDVLDGALDPFMAAALSQKVTGEKVEVEDID

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=225, Percent_Identity=42.2222222222222, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI34577120, Length=273, Percent_Identity=36.2637362637363, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI166795305, Length=147, Percent_Identity=44.8979591836735, Blast_Score=123, Evalue=2e-28,
Organism=Escherichia coli, GI2367172, Length=354, Percent_Identity=52.2598870056497, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI1787462, Length=235, Percent_Identity=44.6808510638298, Blast_Score=187, Evalue=9e-49,
Organism=Caenorhabditis elegans, GI17542784, Length=231, Percent_Identity=32.034632034632, Blast_Score=97, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6321295, Length=299, Percent_Identity=34.1137123745819, Blast_Score=140, Evalue=3e-34,
Organism=Drosophila melanogaster, GI19921226, Length=285, Percent_Identity=37.5438596491228, Blast_Score=161, Evalue=6e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 41451; Mature: 41451

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAEALDHIDKITAALALLRRFLDWDRAVRRLDELNAKVEDPTLWDNPKAAQDVMRERRR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
LDEAITATRAIERECADTAELIELAEMEGDEAMVDEAVASLAALAARAEEDKVKALLAGE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHEEEECC
ADGNDAYIEVHAGAGGTESQDWAEMLQRMYMRWAEKRGMKVELIEYQAGEQAGIKSATML
CCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEE
VKGENAYGYAKTESGVHRLVRISPYDSSARRHTSFSSVWVYPVIDDNIEIEINEGDLKID
EECCCCCCCCCCHHHHEEEEEECCCCCCHHHCCCCCCEEEEEEECCCEEEEECCCCEEEE
TYRASGAGGQHVNTTDSAVRITHIPTGIVVASQNDRSQHKNRATAMGMLKARLYEAELQK
EEEECCCCCCCCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
REAAASGEYQAKTEIGWGHQIRSYVLQPYQLVKDLRTGVTSTAPGDVLDGALDPFMAAAL
HHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
SQKVTGEKVEVEDID
HHHCCCCEEEEECCC
>Mature Secondary Structure
MRAEALDHIDKITAALALLRRFLDWDRAVRRLDELNAKVEDPTLWDNPKAAQDVMRERRR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
LDEAITATRAIERECADTAELIELAEMEGDEAMVDEAVASLAALAARAEEDKVKALLAGE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHEEEECC
ADGNDAYIEVHAGAGGTESQDWAEMLQRMYMRWAEKRGMKVELIEYQAGEQAGIKSATML
CCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEE
VKGENAYGYAKTESGVHRLVRISPYDSSARRHTSFSSVWVYPVIDDNIEIEINEGDLKID
EECCCCCCCCCCHHHHEEEEEECCCCCCHHHCCCCCCEEEEEEECCCEEEEECCCCEEEE
TYRASGAGGQHVNTTDSAVRITHIPTGIVVASQNDRSQHKNRATAMGMLKARLYEAELQK
EEEECCCCCCCCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
REAAASGEYQAKTEIGWGHQIRSYVLQPYQLVKDLRTGVTSTAPGDVLDGALDPFMAAAL
HHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
SQKVTGEKVEVEDID
HHHCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA