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The map label for this gene is hisF

Identifier: 103488621

GI number: 103488621

Start: 3297558

End: 3298325

Strand: Reverse

Name: hisF

Synonym: Sala_3146

Alternate gene names: 103488621

Gene position: 3298325-3297558 (Counterclockwise)

Preceding gene: 103488622

Following gene: 103488619

Centisome position: 98.6

GC content: 72.14

Gene sequence:

>768_bases
ATGACCGTCCGCGTCCGCGTCATCCCCTGCCTCGACGTCGCCGACGGGCGCGTGGTCAAGGGCGTCAATTTCGTCGACCT
GCGCGACGCGGGCGATCCGGTCGAGGCCGCACGCGCCTATGACGCCGCGGGCGCCGACGAGCTTTGCTTCCTCGATATCA
GCGCCAGCCACCAAGGCCGCGGCACCCTGCTCGACCTGGTCAGCCGCACCGCCGAAGTCTGTTTCATGCCGCTCACCGTC
GGCGGCGGGGTGCGCAGCGCCGAGGATGCACGCGCGCTGCTGCTCGCGGGCGCCGACAAGGTGGCGGTGAACAGCGCCGC
GGTCGCGCGGCCCGAACTGGTCGCCGACATTGCCGACCGTTTCGGCAGCCAGTGCGCGGTGGGCAGCATCGACGCGCGCC
GCGTCGAGGATGGCCGCTGGGAAATCTTCACCCACGGCGGGCGCAAGCCCACCGGGATCGACGCAGTGGAGCACGCCGTC
CGCCTTGCGACGCTCGGCGCGGGCGAGCTGCTCGTGACCAGCATGGATCGCGACGGCACCAGGGACGGCTACGACCTCGC
GCTTACCCGCGCGATCGCCGATGCGGTGAGCGTGCCGGTGATCGCGAGCGGCGGCGTCGGCAATCTCGACCATCTGGTCG
CCGGCGTGATCGAGGGCGGCGCGAGCGCGGTGCTCGCGGCGAGCATCTTCCACTTCGGCGAAGCGACGATTGCCGAGGCG
CACGCGCGGCTGGCGGCGGCGGGGTTGCCGGTGCGGTCGCCCATTTGA

Upstream 100 bases:

>100_bases
CCGTTCGTGTCGAGCGAAGTCGAGACACCTCGACGGACAGCGCCATGCTGCCGGGTGTCTCGATTTCGCTCGACACGAAC
GGATTTGGTGGGGTGACCCA

Downstream 100 bases:

>100_bases
TCGGCAGGCTGTGATATGCAGCCTGTGCCCCCGCGAAGGCGGGGGCCGCCAGCAACCTTGCGCTACGCCGACAGCGGTCC
CCGCCTTCGCGGGGACGACG

Product: imidazole glycerol phosphate synthase subunit HisF

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MTVRVRVIPCLDVADGRVVKGVNFVDLRDAGDPVEAARAYDAAGADELCFLDISASHQGRGTLLDLVSRTAEVCFMPLTV
GGGVRSAEDARALLLAGADKVAVNSAAVARPELVADIADRFGSQCAVGSIDARRVEDGRWEIFTHGGRKPTGIDAVEHAV
RLATLGAGELLVTSMDRDGTRDGYDLALTRAIADAVSVPVIASGGVGNLDHLVAGVIEGGASAVLAASIFHFGEATIAEA
HARLAAAGLPVRSPI

Sequences:

>Translated_255_residues
MTVRVRVIPCLDVADGRVVKGVNFVDLRDAGDPVEAARAYDAAGADELCFLDISASHQGRGTLLDLVSRTAEVCFMPLTV
GGGVRSAEDARALLLAGADKVAVNSAAVARPELVADIADRFGSQCAVGSIDARRVEDGRWEIFTHGGRKPTGIDAVEHAV
RLATLGAGELLVTSMDRDGTRDGYDLALTRAIADAVSVPVIASGGVGNLDHLVAGVIEGGASAVLAASIFHFGEATIAEA
HARLAAAGLPVRSPI
>Mature_254_residues
TVRVRVIPCLDVADGRVVKGVNFVDLRDAGDPVEAARAYDAAGADELCFLDISASHQGRGTLLDLVSRTAEVCFMPLTVG
GGVRSAEDARALLLAGADKVAVNSAAVARPELVADIADRFGSQCAVGSIDARRVEDGRWEIFTHGGRKPTGIDAVEHAVR
LATLGAGELLVTSMDRDGTRDGYDLALTRAIADAVSVPVIASGGVGNLDHLVAGVIEGGASAVLAASIFHFGEATIAEAH
ARLAAAGLPVRSPI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=252, Percent_Identity=41.6666666666667, Blast_Score=190, Evalue=6e-50,
Organism=Escherichia coli, GI87082028, Length=240, Percent_Identity=31.6666666666667, Blast_Score=97, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6319725, Length=310, Percent_Identity=30.9677419354839, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_SPHAL (Q1GNC3)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_618182.1
- ProteinModelPortal:   Q1GNC3
- SMR:   Q1GNC3
- GeneID:   4082482
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_3146
- NMPDR:   fig|317655.9.peg.2995
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- ProtClustDB:   PRK02083
- BioCyc:   SALA317655:SALA_3146-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.- [C]

Molecular weight: Translated: 26215; Mature: 26084

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: ACT_SITE 12-12 ACT_SITE 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVRVRVIPCLDVADGRVVKGVNFVDLRDAGDPVEAARAYDAAGADELCFLDISASHQGR
CEEEEEEEEEEECCCCCEECCCCEEEECCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCC
GTLLDLVSRTAEVCFMPLTVGGGVRSAEDARALLLAGADKVAVNSAAVARPELVADIADR
HHHHHHHHHHHHHEEEEEEECCCCCCCCHHEEEEEECCCHHEECCHHHCCHHHHHHHHHH
FGSQCAVGSIDARRVEDGRWEIFTHGGRKPTGIDAVEHAVRLATLGAGELLVTSMDRDGT
CCCCCEECCCCCEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCC
RDGYDLALTRAIADAVSVPVIASGGVGNLDHLVAGVIEGGASAVLAASIFHFGEATIAEA
CCCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHH
HARLAAAGLPVRSPI
HHHHHHCCCCCCCCC
>Mature Secondary Structure 
TVRVRVIPCLDVADGRVVKGVNFVDLRDAGDPVEAARAYDAAGADELCFLDISASHQGR
EEEEEEEEEEECCCCCEECCCCEEEECCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCC
GTLLDLVSRTAEVCFMPLTVGGGVRSAEDARALLLAGADKVAVNSAAVARPELVADIADR
HHHHHHHHHHHHHEEEEEEECCCCCCCCHHEEEEEECCCHHEECCHHHCCHHHHHHHHHH
FGSQCAVGSIDARRVEDGRWEIFTHGGRKPTGIDAVEHAVRLATLGAGELLVTSMDRDGT
CCCCCEECCCCCEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCC
RDGYDLALTRAIADAVSVPVIASGGVGNLDHLVAGVIEGGASAVLAASIFHFGEATIAEA
CCCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHH
HARLAAAGLPVRSPI
HHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA