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The map label for this gene is trmB [H]

Identifier: 103488615

GI number: 103488615

Start: 3292374

End: 3293153

Strand: Direct

Name: trmB [H]

Synonym: Sala_3140

Alternate gene names: 103488615

Gene position: 3292374-3293153 (Clockwise)

Preceding gene: 103488614

Following gene: 103488617

Centisome position: 98.42

GC content: 68.21

Gene sequence:

>780_bases
TTGTCCCGCCCCGCCACGGACCCACTGCGCTTGCCGCCCCCGCTTGCCCGCGCTAACGCGCGCGCCATGACTGCGCACAA
ACCCGGCGATCCGACGACCCTCAACCGCCTTTACGGCCGATCGAAGGGCAAGCCGCTGCGCGCCGGCCAGCAGGATCTGG
TCGATACGCTGTTGCCACAGATCGCGGTGCCGCTGGACGGCGAGGTCACCGCGCGGCGGTTGTTCGGCTTCGACCGCCCG
CTGCATTTCGAAATCGGCTTTGGCGGCGGCGAGCATATGGCGGCGCGCGCCGACATGCTGCCCGACCATGGTTTCATCGG
CGCCGAACCCTTTATCAACGGCGTTGCGCAGGCGCTCGTCCATGTCGCGGGCGATCATGGCCAGCACCCGCCGCTCGGCA
ATGTCCGCATCCACCATGGCGACGCGCTGGAGGTGCTGCGGCGCATCCCCGACGGCGCCCTCGCCTTCGTCTATCTGCTG
CACCCCGACCCCTGGCCCAAGGCGCGCCATGCCAAGCGGCGGATGATGAACGACGGTCCGCTCGACCTGATCGCCGCGAA
GCTCAAACCCGGCGGCGAGTTCCGTTTCGGCACCGACCACCCCATTTACCTCCGGCACGCGCTGATGGTGATGCGCCGTC
ATCGGCATCAGTTCGAATGGCTGGCAAAGGATGCGCGCGATTTTCAGGTGCGGCCCGGCGGCTGGCCCGAAACGCGCTAC
GAAGCCAAGGCGCGCGCACAGGGGCACGAAGTCTGGTATTTCCGCTGGCGGCGTCGCTGA

Upstream 100 bases:

>100_bases
TTCGGCCGCCAGGCCGACGGCGACGCCTTCCCGTGGGAACGCACCGACCTGGTGGACAAGCTGAAAGCCGCGCTCGCGGT
GTGAACCGGGCCGGGGTGCC

Downstream 100 bases:

>100_bases
TCGCCCCGGCCACGCACAAATAAATGGGGCGGCCTCGTATCGAGTGCCGCCCCAGTTTGTGGACCCGTCGGGGGAGAGGG
AGAGGACGGTCCGGAGATTC

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MSRPATDPLRLPPPLARANARAMTAHKPGDPTTLNRLYGRSKGKPLRAGQQDLVDTLLPQIAVPLDGEVTARRLFGFDRP
LHFEIGFGGGEHMAARADMLPDHGFIGAEPFINGVAQALVHVAGDHGQHPPLGNVRIHHGDALEVLRRIPDGALAFVYLL
HPDPWPKARHAKRRMMNDGPLDLIAAKLKPGGEFRFGTDHPIYLRHALMVMRRHRHQFEWLAKDARDFQVRPGGWPETRY
EAKARAQGHEVWYFRWRRR

Sequences:

>Translated_259_residues
MSRPATDPLRLPPPLARANARAMTAHKPGDPTTLNRLYGRSKGKPLRAGQQDLVDTLLPQIAVPLDGEVTARRLFGFDRP
LHFEIGFGGGEHMAARADMLPDHGFIGAEPFINGVAQALVHVAGDHGQHPPLGNVRIHHGDALEVLRRIPDGALAFVYLL
HPDPWPKARHAKRRMMNDGPLDLIAAKLKPGGEFRFGTDHPIYLRHALMVMRRHRHQFEWLAKDARDFQVRPGGWPETRY
EAKARAQGHEVWYFRWRRR
>Mature_258_residues
SRPATDPLRLPPPLARANARAMTAHKPGDPTTLNRLYGRSKGKPLRAGQQDLVDTLLPQIAVPLDGEVTARRLFGFDRPL
HFEIGFGGGEHMAARADMLPDHGFIGAEPFINGVAQALVHVAGDHGQHPPLGNVRIHHGDALEVLRRIPDGALAFVYLLH
PDPWPKARHAKRRMMNDGPLDLIAAKLKPGGEFRFGTDHPIYLRHALMVMRRHRHQFEWLAKDARDFQVRPGGWPETRYE
AKARAQGHEVWYFRWRRR

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=232, Percent_Identity=34.9137931034483, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 29296; Mature: 29165

Theoretical pI: Translated: 11.02; Mature: 11.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRPATDPLRLPPPLARANARAMTAHKPGDPTTLNRLYGRSKGKPLRAGQQDLVDTLLPQ
CCCCCCCCCCCCCCHHHCCCCEEECCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
IAVPLDGEVTARRLFGFDRPLHFEIGFGGGEHMAARADMLPDHGFIGAEPFINGVAQALV
HCCCCCCCHHHHHHHCCCCCEEEEEECCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
HVAGDHGQHPPLGNVRIHHGDALEVLRRIPDGALAFVYLLHPDPWPKARHAKRRMMNDGP
HHHCCCCCCCCCCCEEEECCHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCC
LDLIAAKLKPGGEFRFGTDHPIYLRHALMVMRRHRHQFEWLAKDARDFQVRPGGWPETRY
HHEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHH
EAKARAQGHEVWYFRWRRR
HHHHHCCCCEEEEEEEECC
>Mature Secondary Structure 
SRPATDPLRLPPPLARANARAMTAHKPGDPTTLNRLYGRSKGKPLRAGQQDLVDTLLPQ
CCCCCCCCCCCCCHHHCCCCEEECCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
IAVPLDGEVTARRLFGFDRPLHFEIGFGGGEHMAARADMLPDHGFIGAEPFINGVAQALV
HCCCCCCCHHHHHHHCCCCCEEEEEECCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
HVAGDHGQHPPLGNVRIHHGDALEVLRRIPDGALAFVYLLHPDPWPKARHAKRRMMNDGP
HHHCCCCCCCCCCCEEEECCHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCC
LDLIAAKLKPGGEFRFGTDHPIYLRHALMVMRRHRHQFEWLAKDARDFQVRPGGWPETRY
HHEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHH
EAKARAQGHEVWYFRWRRR
HHHHHCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA