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The map label for this gene is metK

Identifier: 103488614

GI number: 103488614

Start: 3291137

End: 3292357

Strand: Direct

Name: metK

Synonym: Sala_3139

Alternate gene names: 103488614

Gene position: 3291137-3292357 (Clockwise)

Preceding gene: 103488613

Following gene: 103488615

Centisome position: 98.38

GC content: 65.68

Gene sequence:

>1221_bases
ATGCGCAACAGCTTCCTCTTCACCTCCGAAAGCGTGTCCGAAGGACATCCCGACAAGGTGGCCGACCAGATCAGCGATTC
GATCGTCGATCTGTTCCTGGCGAAAGATCCCGAAGCGCGTGTGGCGTGCGAGACGCTGACGACGACGCAGCTCGTCGTGC
TCGCGGGCGAAATCCGCTGCAAGGGCGTGTTCGAGGATGGCGAATGGGCGCCCGGCGCGCTCGACGAGATCGAGGCGACG
GTGCGCCGGACGGTGCGCGAGATCGGCTATGAACAGGCGGGGTTCCACTGGAACCGGTTTCGCTTCGAGAATAATCTGCA
CGGCCAGTCGCCGCAGATCGCGCAGGGCGTTGACGAAGGCGCGGGCAAGGACGAAGGCGCGGGCGACCAGGGGATCATGT
TCGGCTATGCGTCCGACGAAACGCCCGACTTCATGCCCGCGACGCTCGACTACAGCCACAAGATCCTCGAGCGCATGGCA
TCCGACCGCAAGGCGGGGATCGCGCCCTTCCTCGAACCCGATGCGAAAAGCCAGGTCACGTTGCGCTATGCCAACGAACG
CCCGGTCGAGGCGACCGCGATCGTCGTGTCGACGCAGCACGCGCCGGGCTATTATTTCCACAATGGCGAAGGCGACGAGG
CGAAATATACCGAGCTGCGCAAATATGTGCTCGGCGTGATCGCCGACGTGCTGCCCGCCGAACTGCTCACCGCGAACACC
GTCTATCACATCAACCCGACCGGCCGCTTCGAGATCGGCGGGCCCGACGGCGACGCGGGCCTCACGGGGCGCAAGATCAT
CGTCGACACCTATGGCGGCGCGAGCCCGCACGGCGGCGGCGCGTTCAGCGGCAAGGATCCCACCAAGGTCGACCGCTCGG
CGGCCTATATCACGCGCTATCTGGCGAAGAATATCGTCGCCGCGGGCCTCGCGCGCCGCTGCACGATCCAATTGAGCTAC
GCGATCGGCGTCGCCGAGCCGCTGTCGATCTATGTCGACCTCCACGGCACCGGCACGGTCGACGAAGGCCGCATCGAAGC
CGTTCTCCCACAGCTCGTCCGCCTGACGCCCAAGGGCATCCGCACCCACCTTGGCCTCAACAAGCCGATCTATCGCCAGA
CCGCCGCCTATGGCCATTTCGGCCGCCAGGCCGACGGCGACGCCTTCCCGTGGGAACGCACCGACCTGGTGGACAAGCTG
AAAGCCGCGCTCGCGGTGTGA

Upstream 100 bases:

>100_bases
GCGCTCGCGCTGCTCGCGCTGGCCATTGCCTTTCGGCGGCGCGGACGCTAACAGCCACCCAACATAAAGCTTCCTTTATA
TCCCCTCGCAAAGGCCCCCC

Downstream 100 bases:

>100_bases
ACCGGGCCGGGGTGCCTTGTCCCGCCCCGCCACGGACCCACTGCGCTTGCCGCCCCCGCTTGCCCGCGCTAACGCGCGCG
CCATGACTGCGCACAAACCC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MRNSFLFTSESVSEGHPDKVADQISDSIVDLFLAKDPEARVACETLTTTQLVVLAGEIRCKGVFEDGEWAPGALDEIEAT
VRRTVREIGYEQAGFHWNRFRFENNLHGQSPQIAQGVDEGAGKDEGAGDQGIMFGYASDETPDFMPATLDYSHKILERMA
SDRKAGIAPFLEPDAKSQVTLRYANERPVEATAIVVSTQHAPGYYFHNGEGDEAKYTELRKYVLGVIADVLPAELLTANT
VYHINPTGRFEIGGPDGDAGLTGRKIIVDTYGGASPHGGGAFSGKDPTKVDRSAAYITRYLAKNIVAAGLARRCTIQLSY
AIGVAEPLSIYVDLHGTGTVDEGRIEAVLPQLVRLTPKGIRTHLGLNKPIYRQTAAYGHFGRQADGDAFPWERTDLVDKL
KAALAV

Sequences:

>Translated_406_residues
MRNSFLFTSESVSEGHPDKVADQISDSIVDLFLAKDPEARVACETLTTTQLVVLAGEIRCKGVFEDGEWAPGALDEIEAT
VRRTVREIGYEQAGFHWNRFRFENNLHGQSPQIAQGVDEGAGKDEGAGDQGIMFGYASDETPDFMPATLDYSHKILERMA
SDRKAGIAPFLEPDAKSQVTLRYANERPVEATAIVVSTQHAPGYYFHNGEGDEAKYTELRKYVLGVIADVLPAELLTANT
VYHINPTGRFEIGGPDGDAGLTGRKIIVDTYGGASPHGGGAFSGKDPTKVDRSAAYITRYLAKNIVAAGLARRCTIQLSY
AIGVAEPLSIYVDLHGTGTVDEGRIEAVLPQLVRLTPKGIRTHLGLNKPIYRQTAAYGHFGRQADGDAFPWERTDLVDKL
KAALAV
>Mature_406_residues
MRNSFLFTSESVSEGHPDKVADQISDSIVDLFLAKDPEARVACETLTTTQLVVLAGEIRCKGVFEDGEWAPGALDEIEAT
VRRTVREIGYEQAGFHWNRFRFENNLHGQSPQIAQGVDEGAGKDEGAGDQGIMFGYASDETPDFMPATLDYSHKILERMA
SDRKAGIAPFLEPDAKSQVTLRYANERPVEATAIVVSTQHAPGYYFHNGEGDEAKYTELRKYVLGVIADVLPAELLTANT
VYHINPTGRFEIGGPDGDAGLTGRKIIVDTYGGASPHGGGAFSGKDPTKVDRSAAYITRYLAKNIVAAGLARRCTIQLSY
AIGVAEPLSIYVDLHGTGTVDEGRIEAVLPQLVRLTPKGIRTHLGLNKPIYRQTAAYGHFGRQADGDAFPWERTDLVDKL
KAALAV

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=397, Percent_Identity=48.3627204030227, Blast_Score=337, Evalue=1e-92,
Organism=Homo sapiens, GI4557737, Length=396, Percent_Identity=48.989898989899, Blast_Score=335, Evalue=3e-92,
Organism=Escherichia coli, GI1789311, Length=401, Percent_Identity=52.8678304239402, Blast_Score=390, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17538494, Length=400, Percent_Identity=49, Blast_Score=347, Evalue=4e-96,
Organism=Caenorhabditis elegans, GI17538498, Length=400, Percent_Identity=48.75, Blast_Score=345, Evalue=3e-95,
Organism=Caenorhabditis elegans, GI17551082, Length=401, Percent_Identity=48.8778054862843, Blast_Score=343, Evalue=9e-95,
Organism=Caenorhabditis elegans, GI25145633, Length=400, Percent_Identity=48.25, Blast_Score=342, Evalue=3e-94,
Organism=Caenorhabditis elegans, GI32565905, Length=398, Percent_Identity=45.4773869346734, Blast_Score=304, Evalue=7e-83,
Organism=Caenorhabditis elegans, GI32565911, Length=351, Percent_Identity=46.1538461538462, Blast_Score=273, Evalue=8e-74,
Organism=Caenorhabditis elegans, GI25145635, Length=351, Percent_Identity=45.2991452991453, Blast_Score=268, Evalue=5e-72,
Organism=Caenorhabditis elegans, GI32565903, Length=306, Percent_Identity=49.6732026143791, Blast_Score=263, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI32565909, Length=93, Percent_Identity=47.3118279569892, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI32565907, Length=42, Percent_Identity=69.0476190476191, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323209, Length=401, Percent_Identity=47.3815461346633, Blast_Score=327, Evalue=2e-90,
Organism=Saccharomyces cerevisiae, GI6320710, Length=399, Percent_Identity=46.3659147869674, Blast_Score=322, Evalue=5e-89,
Organism=Drosophila melanogaster, GI24580527, Length=397, Percent_Identity=47.6070528967254, Blast_Score=319, Evalue=2e-87,
Organism=Drosophila melanogaster, GI24580525, Length=397, Percent_Identity=47.6070528967254, Blast_Score=319, Evalue=2e-87,
Organism=Drosophila melanogaster, GI45552159, Length=397, Percent_Identity=47.6070528967254, Blast_Score=315, Evalue=3e-86,
Organism=Drosophila melanogaster, GI24580529, Length=397, Percent_Identity=47.6070528967254, Blast_Score=315, Evalue=3e-86,
Organism=Drosophila melanogaster, GI24580537, Length=397, Percent_Identity=47.6070528967254, Blast_Score=315, Evalue=3e-86,
Organism=Drosophila melanogaster, GI24580533, Length=397, Percent_Identity=47.6070528967254, Blast_Score=315, Evalue=3e-86,
Organism=Drosophila melanogaster, GI24580535, Length=397, Percent_Identity=47.6070528967254, Blast_Score=315, Evalue=3e-86,
Organism=Drosophila melanogaster, GI24580531, Length=397, Percent_Identity=47.6070528967254, Blast_Score=315, Evalue=3e-86,
Organism=Drosophila melanogaster, GI24580541, Length=397, Percent_Identity=45.3400503778337, Blast_Score=294, Evalue=8e-80,
Organism=Drosophila melanogaster, GI24580539, Length=130, Percent_Identity=46.1538461538462, Blast_Score=108, Evalue=7e-24,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_SPHAL (Q1GND0)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_618175.1
- ProteinModelPortal:   Q1GND0
- SMR:   Q1GND0
- GeneID:   4082395
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_3139
- NMPDR:   fig|317655.9.peg.2988
- HOGENOM:   HBG443662
- OMA:   TQHAPGY
- ProtClustDB:   PRK05250
- BioCyc:   SALA317655:SALA_3139-MONOMER
- BRENDA:   2.5.1.6
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 44100; Mature: 44100

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNSFLFTSESVSEGHPDKVADQISDSIVDLFLAKDPEARVACETLTTTQLVVLAGEIRC
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHEEEHHHHHEEEEEEECCEEE
KGVFEDGEWAPGALDEIEATVRRTVREIGYEQAGFHWNRFRFENNLHGQSPQIAQGVDEG
EEECCCCCCCCCHHHHHHHHHHHHHHHCCCHHCCCEEEEEEECCCCCCCCCHHHHCCHHC
AGKDEGAGDQGIMFGYASDETPDFMPATLDYSHKILERMASDRKAGIAPFLEPDAKSQVT
CCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEE
LRYANERPVEATAIVVSTQHAPGYYFHNGEGDEAKYTELRKYVLGVIADVLPAELLTANT
EEECCCCCCEEEEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCE
VYHINPTGRFEIGGPDGDAGLTGRKIIVDTYGGASPHGGGAFSGKDPTKVDRSAAYITRY
EEEECCCCEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LAKNIVAAGLARRCTIQLSYAIGVAEPLSIYVDLHGTGTVDEGRIEAVLPQLVRLTPKGI
HHHHHHHHHHHHEEEEEEEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHH
RTHLGLNKPIYRQTAAYGHFGRQADGDAFPWERTDLVDKLKAALAV
HHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRNSFLFTSESVSEGHPDKVADQISDSIVDLFLAKDPEARVACETLTTTQLVVLAGEIRC
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHEEEHHHHHEEEEEEECCEEE
KGVFEDGEWAPGALDEIEATVRRTVREIGYEQAGFHWNRFRFENNLHGQSPQIAQGVDEG
EEECCCCCCCCCHHHHHHHHHHHHHHHCCCHHCCCEEEEEEECCCCCCCCCHHHHCCHHC
AGKDEGAGDQGIMFGYASDETPDFMPATLDYSHKILERMASDRKAGIAPFLEPDAKSQVT
CCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEE
LRYANERPVEATAIVVSTQHAPGYYFHNGEGDEAKYTELRKYVLGVIADVLPAELLTANT
EEECCCCCCEEEEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCE
VYHINPTGRFEIGGPDGDAGLTGRKIIVDTYGGASPHGGGAFSGKDPTKVDRSAAYITRY
EEEECCCCEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LAKNIVAAGLARRCTIQLSYAIGVAEPLSIYVDLHGTGTVDEGRIEAVLPQLVRLTPKGI
HHHHHHHHHHHHEEEEEEEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHH
RTHLGLNKPIYRQTAAYGHFGRQADGDAFPWERTDLVDKLKAALAV
HHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA