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The map label for this gene is emrB [H]

Identifier: 103488592

GI number: 103488592

Start: 3266093

End: 3267643

Strand: Direct

Name: emrB [H]

Synonym: Sala_3117

Alternate gene names: 103488592

Gene position: 3266093-3267643 (Clockwise)

Preceding gene: 103488591

Following gene: 103488593

Centisome position: 97.64

GC content: 66.92

Gene sequence:

>1551_bases
ATGGCCAGCGTCGCCGTCCCCGCCACCGCGATCTCCGCCGCGCCACAACCGCTCACCGGCGTGCGGCTGCTCGTCGCGGC
CTTTGCGCTCGCGCTCGCCAATTTCGTCGTCGTGCTCGACACGACGATCGCCAATGTGTCGGTGCCACATATCGCGGGCG
GGCTGGCCGTGTCGCCGACGCAGGGGACGTGGGTGATCACCAGCTATGCGGTCGCCGACGCGATCAGCGTGCCGCTCACC
GGCTGGCTCGCCTATCGCTTCGGCACGGTGCGCTGGTTCCTCTATTCGATCTTTGGCTTCGGCCTGTTTTCGCTGCTCTG
CGGCGTCGCGCAGACGCTCGACGCGCTCGTCCTCTTCCGCGTCCTTCAGGGCCTGTCGGGCGGACCGCTGATGCCGCTCT
CGCAGATATTGCTGCTGCGCATCTTCCCCAGGGAAAAGGCCGGCATCGCGCTCGCCATCTGGGCGATGACGACGACCACC
GCGCCGATCCTTGGCCCGATTCTGGGCGGGCTGATCAGCGATAATTGGGGGTGGCACTGGATCTTCTTCATCAACCTGCC
GGTCGTCGCGATCTGCGCCTTTGGCGTCGCGACGCTGCTCACGCCGTTCGAGACGCAGCGCGTCAAGGCGCCGATCGACG
TCGTCGGGCTGATCCTGCTCGTCGTCTCGGTCGGCGCCTTCCAGATCATGCTCGACACGGGGCGCGAACATGACTGGTTC
GGGTCGGCGTGGATCGTCACGCTGGCGATCGTCGCCGCGATCGGCTTTGCCGCCTTTGTCATATGGGAACTCACCGACGC
CAACCCGATCGTCAATCTGCGCATCTTCCGCTTTCGCGGTTTCACTTTCGGCACAATCGCGCTGTCGCTGGGGTTCGGCG
CCTTTTTCGCGCAAGTCGTGCTCACGCCGCTGTGGCTGCAACAGGTCGCGGGCTATACCGCGACCGAGACGGGCTATGTC
GTCGCGTGGCTCGGCTGTTTCGCGGTGCTGTTTTCGCCGATCGCAGCCGGAACGCTGGGCAAACTCGACATCCGGCTGAC
GGTGAGCGCGGGCATATTGTGGATGGCGGCGATGTCGATCCTGCGCGCCGGCTGGAACGCCGACGTCGATTATTGGACGC
TCGCGCTGCCGCATGTGTTGCAGGGGATGGGCATGCCCTTCTTCTTCGTTGGGCTGACCGCGCTGGTGCTGAGTTCGGTG
CCGGTCAAGGACCAGACGTCGGCGGCGGGGCTGATGAGCTTCCTGCGTACGCTGAGCGGGGCGATCGGCACTGCGGTCGC
GACGACCGCATGGGACGAGGCGGGGCGGACCTCGCGGTCGGAGCTTGTCGCCTCGCTCAACGATCCGGGCGGGTTCATGG
CCTCGCTCGAACGCGCCGGGCTGACGCTCGAACAGGCGCGCGCGCTGCTCGACCGGCTGGTCGAGGTGCAGGCGCTGACG
CTGGGCGTGATCCACATCTTCCTCGCCGCCGCGGTGGTGTTCGTGATCGCCGCGGCAAGCGTGTGGATCGCGCCCAGACC
CAGGCAGGTGTCGATGGGCGCCGCGCACTAG

Upstream 100 bases:

>100_bases
ACGATCTGGATGGGCGCTCGCAGGAGATTGCCGCGTCGCCCCGGATCAAGTCCGGGGCTCCCCGCAATGACGACGCCTTT
GTCGGGAACGGTAGCCTGAC

Downstream 100 bases:

>100_bases
GGTAGGGTCAGGACGCTGCGCCGCAGGGCTTGGGTCCGCGCCCTTGCCATGCTAGGCGCGCGGCCATGTTGACGATCAAC
GGCCTCACCGTGCGCCTGGG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MASVAVPATAISAAPQPLTGVRLLVAAFALALANFVVVLDTTIANVSVPHIAGGLAVSPTQGTWVITSYAVADAISVPLT
GWLAYRFGTVRWFLYSIFGFGLFSLLCGVAQTLDALVLFRVLQGLSGGPLMPLSQILLLRIFPREKAGIALAIWAMTTTT
APILGPILGGLISDNWGWHWIFFINLPVVAICAFGVATLLTPFETQRVKAPIDVVGLILLVVSVGAFQIMLDTGREHDWF
GSAWIVTLAIVAAIGFAAFVIWELTDANPIVNLRIFRFRGFTFGTIALSLGFGAFFAQVVLTPLWLQQVAGYTATETGYV
VAWLGCFAVLFSPIAAGTLGKLDIRLTVSAGILWMAAMSILRAGWNADVDYWTLALPHVLQGMGMPFFFVGLTALVLSSV
PVKDQTSAAGLMSFLRTLSGAIGTAVATTAWDEAGRTSRSELVASLNDPGGFMASLERAGLTLEQARALLDRLVEVQALT
LGVIHIFLAAAVVFVIAAASVWIAPRPRQVSMGAAH

Sequences:

>Translated_516_residues
MASVAVPATAISAAPQPLTGVRLLVAAFALALANFVVVLDTTIANVSVPHIAGGLAVSPTQGTWVITSYAVADAISVPLT
GWLAYRFGTVRWFLYSIFGFGLFSLLCGVAQTLDALVLFRVLQGLSGGPLMPLSQILLLRIFPREKAGIALAIWAMTTTT
APILGPILGGLISDNWGWHWIFFINLPVVAICAFGVATLLTPFETQRVKAPIDVVGLILLVVSVGAFQIMLDTGREHDWF
GSAWIVTLAIVAAIGFAAFVIWELTDANPIVNLRIFRFRGFTFGTIALSLGFGAFFAQVVLTPLWLQQVAGYTATETGYV
VAWLGCFAVLFSPIAAGTLGKLDIRLTVSAGILWMAAMSILRAGWNADVDYWTLALPHVLQGMGMPFFFVGLTALVLSSV
PVKDQTSAAGLMSFLRTLSGAIGTAVATTAWDEAGRTSRSELVASLNDPGGFMASLERAGLTLEQARALLDRLVEVQALT
LGVIHIFLAAAVVFVIAAASVWIAPRPRQVSMGAAH
>Mature_515_residues
ASVAVPATAISAAPQPLTGVRLLVAAFALALANFVVVLDTTIANVSVPHIAGGLAVSPTQGTWVITSYAVADAISVPLTG
WLAYRFGTVRWFLYSIFGFGLFSLLCGVAQTLDALVLFRVLQGLSGGPLMPLSQILLLRIFPREKAGIALAIWAMTTTTA
PILGPILGGLISDNWGWHWIFFINLPVVAICAFGVATLLTPFETQRVKAPIDVVGLILLVVSVGAFQIMLDTGREHDWFG
SAWIVTLAIVAAIGFAAFVIWELTDANPIVNLRIFRFRGFTFGTIALSLGFGAFFAQVVLTPLWLQQVAGYTATETGYVV
AWLGCFAVLFSPIAAGTLGKLDIRLTVSAGILWMAAMSILRAGWNADVDYWTLALPHVLQGMGMPFFFVGLTALVLSSVP
VKDQTSAAGLMSFLRTLSGAIGTAVATTAWDEAGRTSRSELVASLNDPGGFMASLERAGLTLEQARALLDRLVEVQALTL
GVIHIFLAAAVVFVIAAASVWIAPRPRQVSMGAAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=503, Percent_Identity=43.5387673956262, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI1788710, Length=501, Percent_Identity=39.7205588822355, Blast_Score=343, Evalue=1e-95,
Organism=Escherichia coli, GI1790195, Length=412, Percent_Identity=32.5242718446602, Blast_Score=168, Evalue=8e-43,
Organism=Escherichia coli, GI1788392, Length=401, Percent_Identity=29.1770573566085, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI87081983, Length=402, Percent_Identity=25.3731343283582, Blast_Score=111, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6325455, Length=127, Percent_Identity=29.9212598425197, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321663, Length=125, Percent_Identity=28, Blast_Score=63, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 54732; Mature: 54601

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASVAVPATAISAAPQPLTGVRLLVAAFALALANFVVVLDTTIANVSVPHIAGGLAVSPT
CCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEEHHHHCCCCCHHCCCEEECCC
QGTWVITSYAVADAISVPLTGWLAYRFGTVRWFLYSIFGFGLFSLLCGVAQTLDALVLFR
CCCEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLQGLSGGPLMPLSQILLLRIFPREKAGIALAIWAMTTTTAPILGPILGGLISDNWGWHW
HHCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEHHHCCHHHHHHHHHHHHCCCCCEEE
IFFINLPVVAICAFGVATLLTPFETQRVKAPIDVVGLILLVVSVGAFQIMLDTGREHDWF
EEEEHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCC
GSAWIVTLAIVAAIGFAAFVIWELTDANPIVNLRIFRFRGFTFGTIALSLGFGAFFAQVV
CHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHH
LTPLWLQQVAGYTATETGYVVAWLGCFAVLFSPIAAGTLGKLDIRLTVSAGILWMAAMSI
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHH
LRAGWNADVDYWTLALPHVLQGMGMPFFFVGLTALVLSSVPVKDQTSAAGLMSFLRTLSG
HHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
AIGTAVATTAWDEAGRTSRSELVASLNDPGGFMASLERAGLTLEQARALLDRLVEVQALT
HHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LGVIHIFLAAAVVFVIAAASVWIAPRPRQVSMGAAH
HHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCC
>Mature Secondary Structure 
ASVAVPATAISAAPQPLTGVRLLVAAFALALANFVVVLDTTIANVSVPHIAGGLAVSPT
CCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEEHHHHCCCCCHHCCCEEECCC
QGTWVITSYAVADAISVPLTGWLAYRFGTVRWFLYSIFGFGLFSLLCGVAQTLDALVLFR
CCCEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLQGLSGGPLMPLSQILLLRIFPREKAGIALAIWAMTTTTAPILGPILGGLISDNWGWHW
HHCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEHHHCCHHHHHHHHHHHHCCCCCEEE
IFFINLPVVAICAFGVATLLTPFETQRVKAPIDVVGLILLVVSVGAFQIMLDTGREHDWF
EEEEHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCC
GSAWIVTLAIVAAIGFAAFVIWELTDANPIVNLRIFRFRGFTFGTIALSLGFGAFFAQVV
CHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHH
LTPLWLQQVAGYTATETGYVVAWLGCFAVLFSPIAAGTLGKLDIRLTVSAGILWMAAMSI
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHH
LRAGWNADVDYWTLALPHVLQGMGMPFFFVGLTALVLSSVPVKDQTSAAGLMSFLRTLSG
HHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
AIGTAVATTAWDEAGRTSRSELVASLNDPGGFMASLERAGLTLEQARALLDRLVEVQALT
HHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LGVIHIFLAAAVVFVIAAASVWIAPRPRQVSMGAAH
HHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]