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The map label for this gene is proP [H]

Identifier: 103488432

GI number: 103488432

Start: 3097826

End: 3099160

Strand: Direct

Name: proP [H]

Synonym: Sala_2955

Alternate gene names: 103488432

Gene position: 3097826-3099160 (Clockwise)

Preceding gene: 103488431

Following gene: 103488435

Centisome position: 92.61

GC content: 65.77

Gene sequence:

>1335_bases
ATGGACAAGACGCTGCCCCCCCACGCCCCCGGCCATCCGCCGCCGTTTCGCGAACCATCGCCGCTCCACCGCGCGGTCGG
CGCCTCGGCGCTCGGCAATGCGGTCGAATGGTTCGACTATGGCATCTATGCCTATGGCGTGACCTATATCTCGGCGGCGC
TCTTTCCCGGCGAGACCGAGGAGGCGGTGCTGTTCGCGCTCGCGACCTTTGCCATCTCCTTCCTCGTGCGACCGCTCGGC
GGGCTGTTCTGGGGCCCGCTTGGCGACCGCATCGGGCGGAAATCGGTGCTCGCGATGACGATCCTCTTGATGGCGGGCGC
GACCTTTGCCGTCGGACTCATCCCGTCCTATGACAGCATCGGCTTCTGGGCGCCGACCCTGCTCATCATCCTGCGCATGG
TGCAGGGTTTTTCGACCGGCGGCGAATATGGCGGTGCGGCGACCTTCATGGCCGAATATGCGCCCGACCACCGGCGCGGC
TTCTATGGCAGCTTCCTCGAGTTCGGGACGCTCGCGGGCTTTTCCTTCGGCGCGGCGCTGATGCTCGGCTATTCGCTCTA
TCTGGGCGATGCGGCGATGCACGAATGGGGCTGGCGCATCCCCTTCCTCATCGCCGGGCCGATCGGCCTCATCGGCATGT
ATGTGCGCTCGAAGATGGAGGATACGCCAGTGTTCCGCGCGGAAATGGCGTCGGCCGAGCGGCGCGAACCGCCGGGACTG
CTTGCGCTGATGCGCGATTACTGGCGCCCCTTGCTCGTCGTCGGCGGCCTGGTGGTGGCGCTCAACGTCGTCAACTATTC
GCTGCTCAGCTACATGCCCACCTATCTTCAGCGGCGCATCGGCCTGTCGAGCGAGGAAGCGCTGCTCGTGCCGATCATCG
GCATGGTCTTCATGATGTGCTTCCTGCCCTTCGCCGGCGCGCTGTCGGACCGGATCGGGCGGCGGCCGATGTGGCGCTGG
TCGCTGATCGGCCTCTTCCTGCTCGTCGTGCCGCTCTACATGCTCATCGGCACGGGCTTTGCGGGGGCGATCGTCGCCTT
CATGGCGCTCGGCCTTCTCTATGTGCCGCAGCTCGCGACGATCTCGGCGACCTTTCCCGCGATGTTCCCGACCGCGGTGC
GCTTCGCGGGCTTTGCCATCGCCTATAATATCTCCACCTCGATCTTCGGTGGCACCGCGCCGATGATCGGCAGCGGCCTG
ATCAGTCTGACCGGCGATCCGCTGATGCCCGCCTATTATATGATGCTCGCCTGCCTCGTCGGGCTGGTTGCGCTCCGTTT
CATGCCCGAAACCGCGGGGCGTTCGCTGCTCGAAGACCCGTTCCGGCGGGATTGA

Upstream 100 bases:

>100_bases
CGCGGCGCAGCAGGATTTCCAGATCCTGCCGCGCACGACGCCCGTTGCCGCGCTGCTCGACCCCGCCGGCCCCGAACCCA
ACCCGGAAAACAGGTGATCC

Downstream 100 bases:

>100_bases
CGGGGACTCAGCGCCGCCAGCCGCGCGCGCGCGCGGCGCGTTCGGCGGCGGCGTCAAAAGGTTCGCCGACCATCATCGCC
GCCGCGTCGGCCGCCAGCGC

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MDKTLPPHAPGHPPPFREPSPLHRAVGASALGNAVEWFDYGIYAYGVTYISAALFPGETEEAVLFALATFAISFLVRPLG
GLFWGPLGDRIGRKSVLAMTILLMAGATFAVGLIPSYDSIGFWAPTLLIILRMVQGFSTGGEYGGAATFMAEYAPDHRRG
FYGSFLEFGTLAGFSFGAALMLGYSLYLGDAAMHEWGWRIPFLIAGPIGLIGMYVRSKMEDTPVFRAEMASAERREPPGL
LALMRDYWRPLLVVGGLVVALNVVNYSLLSYMPTYLQRRIGLSSEEALLVPIIGMVFMMCFLPFAGALSDRIGRRPMWRW
SLIGLFLLVVPLYMLIGTGFAGAIVAFMALGLLYVPQLATISATFPAMFPTAVRFAGFAIAYNISTSIFGGTAPMIGSGL
ISLTGDPLMPAYYMMLACLVGLVALRFMPETAGRSLLEDPFRRD

Sequences:

>Translated_444_residues
MDKTLPPHAPGHPPPFREPSPLHRAVGASALGNAVEWFDYGIYAYGVTYISAALFPGETEEAVLFALATFAISFLVRPLG
GLFWGPLGDRIGRKSVLAMTILLMAGATFAVGLIPSYDSIGFWAPTLLIILRMVQGFSTGGEYGGAATFMAEYAPDHRRG
FYGSFLEFGTLAGFSFGAALMLGYSLYLGDAAMHEWGWRIPFLIAGPIGLIGMYVRSKMEDTPVFRAEMASAERREPPGL
LALMRDYWRPLLVVGGLVVALNVVNYSLLSYMPTYLQRRIGLSSEEALLVPIIGMVFMMCFLPFAGALSDRIGRRPMWRW
SLIGLFLLVVPLYMLIGTGFAGAIVAFMALGLLYVPQLATISATFPAMFPTAVRFAGFAIAYNISTSIFGGTAPMIGSGL
ISLTGDPLMPAYYMMLACLVGLVALRFMPETAGRSLLEDPFRRD
>Mature_444_residues
MDKTLPPHAPGHPPPFREPSPLHRAVGASALGNAVEWFDYGIYAYGVTYISAALFPGETEEAVLFALATFAISFLVRPLG
GLFWGPLGDRIGRKSVLAMTILLMAGATFAVGLIPSYDSIGFWAPTLLIILRMVQGFSTGGEYGGAATFMAEYAPDHRRG
FYGSFLEFGTLAGFSFGAALMLGYSLYLGDAAMHEWGWRIPFLIAGPIGLIGMYVRSKMEDTPVFRAEMASAERREPPGL
LALMRDYWRPLLVVGGLVVALNVVNYSLLSYMPTYLQRRIGLSSEEALLVPIIGMVFMMCFLPFAGALSDRIGRRPMWRW
SLIGLFLLVVPLYMLIGTGFAGAIVAFMALGLLYVPQLATISATFPAMFPTAVRFAGFAIAYNISTSIFGGTAPMIGSGL
ISLTGDPLMPAYYMMLACLVGLVALRFMPETAGRSLLEDPFRRD

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=429, Percent_Identity=43.8228438228438, Blast_Score=367, Evalue=1e-102,
Organism=Escherichia coli, GI1788942, Length=414, Percent_Identity=32.1256038647343, Blast_Score=195, Evalue=5e-51,
Organism=Escherichia coli, GI1788292, Length=439, Percent_Identity=30.751708428246, Blast_Score=185, Evalue=5e-48,
Organism=Escherichia coli, GI1789941, Length=435, Percent_Identity=31.9540229885057, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI87082231, Length=208, Percent_Identity=29.8076923076923, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87082404, Length=382, Percent_Identity=23.0366492146597, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 48216; Mature: 48216

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKTLPPHAPGHPPPFREPSPLHRAVGASALGNAVEWFDYGIYAYGVTYISAALFPGETE
CCCCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
EAVLFALATFAISFLVRPLGGLFWGPLGDRIGRKSVLAMTILLMAGATFAVGLIPSYDSI
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCC
GFWAPTLLIILRMVQGFSTGGEYGGAATFMAEYAPDHRRGFYGSFLEFGTLAGFSFGAAL
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHH
MLGYSLYLGDAAMHEWGWRIPFLIAGPIGLIGMYVRSKMEDTPVFRAEMASAERREPPGL
HHHHHHHHCCHHHHHCCCCCCCCEECHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHH
LALMRDYWRPLLVVGGLVVALNVVNYSLLSYMPTYLQRRIGLSSEEALLVPIIGMVFMMC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
FLPFAGALSDRIGRRPMWRWSLIGLFLLVVPLYMLIGTGFAGAIVAFMALGLLYVPQLAT
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ISATFPAMFPTAVRFAGFAIAYNISTSIFGGTAPMIGSGLISLTGDPLMPAYYMMLACLV
HHHHHHHHHHHHHHHHHHHEEEECCHHHCCCCCHHHHCCHHCCCCCCCHHHHHHHHHHHH
GLVALRFMPETAGRSLLEDPFRRD
HHHHHHHCCCHHCHHHHCCCCCCC
>Mature Secondary Structure
MDKTLPPHAPGHPPPFREPSPLHRAVGASALGNAVEWFDYGIYAYGVTYISAALFPGETE
CCCCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
EAVLFALATFAISFLVRPLGGLFWGPLGDRIGRKSVLAMTILLMAGATFAVGLIPSYDSI
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCC
GFWAPTLLIILRMVQGFSTGGEYGGAATFMAEYAPDHRRGFYGSFLEFGTLAGFSFGAAL
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHH
MLGYSLYLGDAAMHEWGWRIPFLIAGPIGLIGMYVRSKMEDTPVFRAEMASAERREPPGL
HHHHHHHHCCHHHHHCCCCCCCCEECHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHH
LALMRDYWRPLLVVGGLVVALNVVNYSLLSYMPTYLQRRIGLSSEEALLVPIIGMVFMMC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
FLPFAGALSDRIGRRPMWRWSLIGLFLLVVPLYMLIGTGFAGAIVAFMALGLLYVPQLAT
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ISATFPAMFPTAVRFAGFAIAYNISTSIFGGTAPMIGSGLISLTGDPLMPAYYMMLACLV
HHHHHHHHHHHHHHHHHHHEEEECCHHHCCCCCHHHHCCHHCCCCCCCHHHHHHHHHHHH
GLVALRFMPETAGRSLLEDPFRRD
HHHHHHHCCCHHCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]