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The map label for this gene is gltX

Identifier: 103488422

GI number: 103488422

Start: 3088458

End: 3089777

Strand: Direct

Name: gltX

Synonym: Sala_2945

Alternate gene names: 103488422

Gene position: 3088458-3089777 (Clockwise)

Preceding gene: 103488421

Following gene: 103488423

Centisome position: 92.33

GC content: 69.32

Gene sequence:

>1320_bases
ATGACCGTCACCACCCGCTTCGCTCCCTCGCCGACCGGCCATCTGCACGTCGGAAACGTCCGTACCGCGCTGCACAACTG
GCTGTGGGCAAGGAAGCATGGCGGGCGTTTCCTGCTGCGCATCGACGATACCGATGTCGAGCGGTCGAAGGAAGACTATG
TCGCGGGCATCCGCGCCGACCTTGCGTGGCTCGGCCTCGACATTGACGCCGAGGAGCGGCAGTCGGCGCGCTTCGCGCTC
TATGAGGCCGAGTTCGAGAAATTGAAGGCGGCCGGCCGCGTCTATGCCTGTTACGAAACCCCCGACGAGCTGGAGGTCCG
CCGCAAGATCCTGCTGTCGCGCGGGCTGCCACCCGTTTACGAACGCAAGCCCGCCGATGCGCCGGTGCCCGAAGGCGTTG
CGCCGCACTGGCGCTTCCGGCTCGATCATGACGCGCCGATCGAATGGACCGACCTCATTCGCGGACCGCAGCATTTCGAG
CCCACGACGATGTCGGACCCGGTGGTGCGCCGCGCCGACGGCAGCTGGCTTTATCTGCTGCCGAGCGTGATCGACGACAT
CGCGATGGGCATCAGCCATGTCGTGCGCGGCGAGGATCATGTGTCGAACACCGCCGCGCAGGTTCAGATGTTCGCCGCGC
TCGGCGCATCGCCCCCCGCCTTTGCCCACGAGGCGCTGCTCGTCGGGACCGAGGGCAAGTTGTCGAAGCGATTGGGCTCG
CTCGGCATGGCGAGCCTGCGCGAACAGGGGATCGAGCCGATCGCGCTCGCCGCCCTGCTTGCGCGCCTCGGCACCAGCGA
TCCGGTCGAGCCGGTGACCGATCTGGCGCCGCTGGTCGCGAGCATCGATTTTGCGCGTTTCGGCCGCGCGCCCGCGCGCT
TCGACGAGGCCGAACTGGCGCTGCTCAATCAGAAGATCCTGCACCATATCGATCATGCCGCGGTGGCGGATCGCCTGCCC
GCGGCGATCGACGAGGCGCGCTGGAAGGCGATTCGCCCCAATCTGACGACCGTTGCCGAGGCCGCCGACTGGCTGCCGGT
ATTCGACGGGCCGTTCGTCCCGACCGCGACCGAGGCCGCCGACCGTCCGGTGCTGCGTGCCGCGGCGAAGGCGGCGCTGG
ACATCGACTGGAGCGCCGACCCCTGGCACGCGCTCACCGCCGCGGTGAAGGAGGCGACGGGGGCGAAGGGCCGGGCGCTG
TTCCTGCCGCTGCGCCGCGCGCTCACCGGGCGCGACCATGGCCCCGACATGGCCGAGCTGCTGCCGCTGATCGCGAAGGA
AGCGGCGATCGCGCGCCTGACCTCGGCCGGGGGTTCGTAA

Upstream 100 bases:

>100_bases
TCTCGCTGCTCGCGATCGCCTGGGCCTTTTGGCCGGTGCGCGACGGCGCGGCGCGATAAATATCGCCATCCGGCGGGCAA
TGCGGTTATAGGCGCGCGCC

Downstream 100 bases:

>100_bases
GGAACCCGCCATTGCGTCCCTGCCTTTGCAGGGGCACAGGGGAAGGCCTCCCCCAAGCAATTCACTTGACAATGACGTGA
TGTTATAACATCATTATCTC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 2; GluRS 2

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MTVTTRFAPSPTGHLHVGNVRTALHNWLWARKHGGRFLLRIDDTDVERSKEDYVAGIRADLAWLGLDIDAEERQSARFAL
YEAEFEKLKAAGRVYACYETPDELEVRRKILLSRGLPPVYERKPADAPVPEGVAPHWRFRLDHDAPIEWTDLIRGPQHFE
PTTMSDPVVRRADGSWLYLLPSVIDDIAMGISHVVRGEDHVSNTAAQVQMFAALGASPPAFAHEALLVGTEGKLSKRLGS
LGMASLREQGIEPIALAALLARLGTSDPVEPVTDLAPLVASIDFARFGRAPARFDEAELALLNQKILHHIDHAAVADRLP
AAIDEARWKAIRPNLTTVAEAADWLPVFDGPFVPTATEAADRPVLRAAAKAALDIDWSADPWHALTAAVKEATGAKGRAL
FLPLRRALTGRDHGPDMAELLPLIAKEAAIARLTSAGGS

Sequences:

>Translated_439_residues
MTVTTRFAPSPTGHLHVGNVRTALHNWLWARKHGGRFLLRIDDTDVERSKEDYVAGIRADLAWLGLDIDAEERQSARFAL
YEAEFEKLKAAGRVYACYETPDELEVRRKILLSRGLPPVYERKPADAPVPEGVAPHWRFRLDHDAPIEWTDLIRGPQHFE
PTTMSDPVVRRADGSWLYLLPSVIDDIAMGISHVVRGEDHVSNTAAQVQMFAALGASPPAFAHEALLVGTEGKLSKRLGS
LGMASLREQGIEPIALAALLARLGTSDPVEPVTDLAPLVASIDFARFGRAPARFDEAELALLNQKILHHIDHAAVADRLP
AAIDEARWKAIRPNLTTVAEAADWLPVFDGPFVPTATEAADRPVLRAAAKAALDIDWSADPWHALTAAVKEATGAKGRAL
FLPLRRALTGRDHGPDMAELLPLIAKEAAIARLTSAGGS
>Mature_438_residues
TVTTRFAPSPTGHLHVGNVRTALHNWLWARKHGGRFLLRIDDTDVERSKEDYVAGIRADLAWLGLDIDAEERQSARFALY
EAEFEKLKAAGRVYACYETPDELEVRRKILLSRGLPPVYERKPADAPVPEGVAPHWRFRLDHDAPIEWTDLIRGPQHFEP
TTMSDPVVRRADGSWLYLLPSVIDDIAMGISHVVRGEDHVSNTAAQVQMFAALGASPPAFAHEALLVGTEGKLSKRLGSL
GMASLREQGIEPIALAALLARLGTSDPVEPVTDLAPLVASIDFARFGRAPARFDEAELALLNQKILHHIDHAAVADRLPA
AIDEARWKAIRPNLTTVAEAADWLPVFDGPFVPTATEAADRPVLRAAAKAALDIDWSADPWHALTAAVKEATGAKGRALF
LPLRRALTGRDHGPDMAELLPLIAKEAAIARLTSAGGS

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=283, Percent_Identity=36.7491166077738, Blast_Score=162, Evalue=5e-40,
Organism=Escherichia coli, GI1788743, Length=467, Percent_Identity=32.762312633833, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI226510925, Length=235, Percent_Identity=32.7659574468085, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786895, Length=140, Percent_Identity=31.4285714285714, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71993394, Length=309, Percent_Identity=34.3042071197411, Blast_Score=156, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6324540, Length=329, Percent_Identity=31.9148936170213, Blast_Score=155, Evalue=8e-39,
Organism=Saccharomyces cerevisiae, GI6321192, Length=235, Percent_Identity=26.8085106382979, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324742, Length=108, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24665321, Length=491, Percent_Identity=29.7352342158859, Blast_Score=181, Evalue=9e-46,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE2_SPHAL (Q1GNX2)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_617983.1
- ProteinModelPortal:   Q1GNX2
- SMR:   Q1GNX2
- GeneID:   4080987
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_2945
- NMPDR:   fig|317655.9.peg.2802
- HOGENOM:   HBG628189
- OMA:   PHWRFKL
- ProtClustDB:   PRK12558
- BioCyc:   SALA317655:SALA_2945-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 47853; Mature: 47722

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 236-236

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTTRFAPSPTGHLHVGNVRTALHNWLWARKHGGRFLLRIDDTDVERSKEDYVAGIRAD
CCEECCCCCCCCCCEEECHHHHHHHHHHHHEECCCEEEEEECCCCCCCCHHHHHHHHHHH
LAWLGLDIDAEERQSARFALYEAEFEKLKAAGRVYACYETPDELEVRRKILLSRGLPPVY
HEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCCHH
ERKPADAPVPEGVAPHWRFRLDHDAPIEWTDLIRGPQHFEPTTMSDPVVRRADGSWLYLL
CCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHCCCCCCCCCCCCCCHHEECCCCEEEEH
PSVIDDIAMGISHVVRGEDHVSNTAAQVQMFAALGASPPAFAHEALLVGTEGKLSKRLGS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHCCEEEECCCCHHHHHHHH
LGMASLREQGIEPIALAALLARLGTSDPVEPVTDLAPLVASIDFARFGRAPARFDEAELA
HHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
LLNQKILHHIDHAAVADRLPAAIDEARWKAIRPNLTTVAEAADWLPVFDGPFVPTATEAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCHHH
DRPVLRAAAKAALDIDWSADPWHALTAAVKEATGAKGRALFLPLRRALTGRDHGPDMAEL
CCHHHHHHHHHHEECCCCCCHHHHHHHHHHHHCCCCCCEEHHHHHHHHCCCCCCCCHHHH
LPLIAKEAAIARLTSAGGS
HHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TVTTRFAPSPTGHLHVGNVRTALHNWLWARKHGGRFLLRIDDTDVERSKEDYVAGIRAD
CEECCCCCCCCCCEEECHHHHHHHHHHHHEECCCEEEEEECCCCCCCCHHHHHHHHHHH
LAWLGLDIDAEERQSARFALYEAEFEKLKAAGRVYACYETPDELEVRRKILLSRGLPPVY
HEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCCHH
ERKPADAPVPEGVAPHWRFRLDHDAPIEWTDLIRGPQHFEPTTMSDPVVRRADGSWLYLL
CCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHCCCCCCCCCCCCCCHHEECCCCEEEEH
PSVIDDIAMGISHVVRGEDHVSNTAAQVQMFAALGASPPAFAHEALLVGTEGKLSKRLGS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHCCEEEECCCCHHHHHHHH
LGMASLREQGIEPIALAALLARLGTSDPVEPVTDLAPLVASIDFARFGRAPARFDEAELA
HHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
LLNQKILHHIDHAAVADRLPAAIDEARWKAIRPNLTTVAEAADWLPVFDGPFVPTATEAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCHHH
DRPVLRAAAKAALDIDWSADPWHALTAAVKEATGAKGRALFLPLRRALTGRDHGPDMAEL
CCHHHHHHHHHHEECCCCCCHHHHHHHHHHHHCCCCCCEEHHHHHHHHCCCCCCCCHHHH
LPLIAKEAAIARLTSAGGS
HHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA