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The map label for this gene is nadE [H]

Identifier: 103488420

GI number: 103488420

Start: 3086293

End: 3087963

Strand: Direct

Name: nadE [H]

Synonym: Sala_2943

Alternate gene names: 103488420

Gene position: 3086293-3087963 (Clockwise)

Preceding gene: 103488415

Following gene: 103488421

Centisome position: 92.26

GC content: 66.91

Gene sequence:

>1671_bases
ATGACCGACGCCGCTGCAACGCTGACCATCGTGCTTTCCCAGATGACCCAGGCGGTCGGCGACCTCGCCGCCAATGCCGA
TGCGATGCGCAGCGTTCGCGCGCGGCACCGCGATGCCGACCTGATCCTTTATCCCGAGCTGCAATTGATTGGCTATCCGC
CCGAGGATCTGGTGCTGAAACCCGCGCTGGCGGAGCGCGCCGCAACGCTGCTCGCCGAACTGGCCGCCGACACCGCCGAT
GGCGGCCCGGCGATGCTGGTGGGATCGGTCGAGCGCGACGCTGACGGCAGCCTCTATAATATCGTCGCGCTGCTCGACGG
CGGGCGGGTGGTCGCGGTGCGGCGCAAGCATGAACTGCCCAATTACGGCACCTTCGACGAAAAGCGCGTCTTCGCACCCG
GCCCGCTGCCCGACATCGTCGAATGGCGCGGGGTGAAGCTGGGCCTGCCGATCTGCGAGGATGGCTGGCTGCCGAAGGTG
AGCGCGCATCTCGCCGCGCAGGGCGCCGAACTGCTGATCTCGGTCAACGGCAGTCCCTATGAAATCGACAAGGACGAGCG
GCGGCTCTCGCAGGTGTTCGCGCGCCGCGTCGCCGAAACCGGATTGCCGCTGATCTTTCTCAACCGCATCGGCGGACAGG
ACGAGCTGGTGTTCGATGGCTGCTCCTTCGTGCTGAACGGCGACGGCACCGCCGCGCACCGCCTGACCGACTGGGAAGCG
GAGGAGCGGGTGACGCGCTGGACGAAGGGCGCGGCGGGTTGGGCCTGCGACGCGGGGGCGATCGCCGAATGGGAGGCGCA
CCCCGCCGACATCTACAGCGCGATGATCCTCAGCTTGCGCGACTATGTGACACGCAACCGCTTCCCCGGCGTCGTGCTCG
GCCTGTCGGGCGGGATCGATTCGGCGATCTGCGCGGCGATCGCCGCCGACGCGCTGGGCCCCGACAAGGTCTGGTGCGTG
ATGCTGCCGAGCCGCTTCACCAGCCAGGCAAGCCTCGATGACGCGGCTGGCTGCGCGCGGATGATCGGATGCCGGCTCGA
CACGATTCCCATCGTGCCCGCCGTGGAGGCCTTCGATCAGATGCTCGCGGGCAGCTTTGCCGATGCCGAGGTCGACATCA
CCGAGGAAAATGTCCAGTCGCGCATCCGCGGCGTGACGTTGATGGCGCTCAGCAACAAGTTCGGCCCGATGCTGCTGACG
ACGGGCAACAAGAGCGAGATGAGCGTCGGCTATGCGACCATCTATGGCGACATGGCGGGCGGCTATAATCCGCTGAAGGA
CGCATACAAGATGACGGTCTTTGCGCTCGCGAAATGGCGCAATGAACATGTGCCGCGGCTGTCGCGCAACCCGGTTGCCC
CCGTGATGCCCGATACGATCATCACCAAGCCGCCGAGCGCCGAGCTGCGCCCCGACCAGAAGGATCAGGACAGCCTGCCG
CCCTATGAGGATCTCGACCGGATGCTCCATATGCTCGTCGAGGAGGAAGCGGGCGTTGACGATGTCGTGGCGCGCCACGG
CTTCGACCGCGACGCGGTGGCGCGGATCGAACGGCTGCTCGCCATCGCCGAGTACAAGCGCCGCCAGGCGCCGCCGGGGG
TCAAATTATCGACGCGCAACTTCGGTCGCGACCGGCGCTATCCGATCACGCACGGGTTCCGGACGGGATAG

Upstream 100 bases:

>100_bases
AGGCGATCGGTCCCCGTCGCCGGCGCATTTGACTGCGTCGTCGCGCTCCCTATCTGCTTGGTCGTTGCAATCGCCTGCGC
CGGGGCTAAGTCTCGCCCCC

Downstream 100 bases:

>100_bases
GGTTTTCAGGGGAAGAAAGGGAAAAGGACGATGCTGCTATCGGGGTCGCAGGCACCGGCAGACCGTGCCGAACGGGTGCT
CGACATATTGCGGATCACCG

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 556; Mature: 555

Protein sequence:

>556_residues
MTDAAATLTIVLSQMTQAVGDLAANADAMRSVRARHRDADLILYPELQLIGYPPEDLVLKPALAERAATLLAELAADTAD
GGPAMLVGSVERDADGSLYNIVALLDGGRVVAVRRKHELPNYGTFDEKRVFAPGPLPDIVEWRGVKLGLPICEDGWLPKV
SAHLAAQGAELLISVNGSPYEIDKDERRLSQVFARRVAETGLPLIFLNRIGGQDELVFDGCSFVLNGDGTAAHRLTDWEA
EERVTRWTKGAAGWACDAGAIAEWEAHPADIYSAMILSLRDYVTRNRFPGVVLGLSGGIDSAICAAIAADALGPDKVWCV
MLPSRFTSQASLDDAAGCARMIGCRLDTIPIVPAVEAFDQMLAGSFADAEVDITEENVQSRIRGVTLMALSNKFGPMLLT
TGNKSEMSVGYATIYGDMAGGYNPLKDAYKMTVFALAKWRNEHVPRLSRNPVAPVMPDTIITKPPSAELRPDQKDQDSLP
PYEDLDRMLHMLVEEEAGVDDVVARHGFDRDAVARIERLLAIAEYKRRQAPPGVKLSTRNFGRDRRYPITHGFRTG

Sequences:

>Translated_556_residues
MTDAAATLTIVLSQMTQAVGDLAANADAMRSVRARHRDADLILYPELQLIGYPPEDLVLKPALAERAATLLAELAADTAD
GGPAMLVGSVERDADGSLYNIVALLDGGRVVAVRRKHELPNYGTFDEKRVFAPGPLPDIVEWRGVKLGLPICEDGWLPKV
SAHLAAQGAELLISVNGSPYEIDKDERRLSQVFARRVAETGLPLIFLNRIGGQDELVFDGCSFVLNGDGTAAHRLTDWEA
EERVTRWTKGAAGWACDAGAIAEWEAHPADIYSAMILSLRDYVTRNRFPGVVLGLSGGIDSAICAAIAADALGPDKVWCV
MLPSRFTSQASLDDAAGCARMIGCRLDTIPIVPAVEAFDQMLAGSFADAEVDITEENVQSRIRGVTLMALSNKFGPMLLT
TGNKSEMSVGYATIYGDMAGGYNPLKDAYKMTVFALAKWRNEHVPRLSRNPVAPVMPDTIITKPPSAELRPDQKDQDSLP
PYEDLDRMLHMLVEEEAGVDDVVARHGFDRDAVARIERLLAIAEYKRRQAPPGVKLSTRNFGRDRRYPITHGFRTG
>Mature_555_residues
TDAAATLTIVLSQMTQAVGDLAANADAMRSVRARHRDADLILYPELQLIGYPPEDLVLKPALAERAATLLAELAADTADG
GPAMLVGSVERDADGSLYNIVALLDGGRVVAVRRKHELPNYGTFDEKRVFAPGPLPDIVEWRGVKLGLPICEDGWLPKVS
AHLAAQGAELLISVNGSPYEIDKDERRLSQVFARRVAETGLPLIFLNRIGGQDELVFDGCSFVLNGDGTAAHRLTDWEAE
ERVTRWTKGAAGWACDAGAIAEWEAHPADIYSAMILSLRDYVTRNRFPGVVLGLSGGIDSAICAAIAADALGPDKVWCVM
LPSRFTSQASLDDAAGCARMIGCRLDTIPIVPAVEAFDQMLAGSFADAEVDITEENVQSRIRGVTLMALSNKFGPMLLTT
GNKSEMSVGYATIYGDMAGGYNPLKDAYKMTVFALAKWRNEHVPRLSRNPVAPVMPDTIITKPPSAELRPDQKDQDSLPP
YEDLDRMLHMLVEEEAGVDDVVARHGFDRDAVARIERLLAIAEYKRRQAPPGVKLSTRNFGRDRRYPITHGFRTG

Specific function: It is not known if this NAD synthase uses NH(3) or glutamine as the N donor [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 60478; Mature: 60346

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDAAATLTIVLSQMTQAVGDLAANADAMRSVRARHRDADLILYPELQLIGYPPEDLVLK
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEECCEEEECCCCHHHEEC
PALAERAATLLAELAADTADGGPAMLVGSVERDADGSLYNIVALLDGGRVVAVRRKHELP
HHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEEEEECCCEEEEEEECCCCC
NYGTFDEKRVFAPGPLPDIVEWRGVKLGLPICEDGWLPKVSAHLAAQGAELLISVNGSPY
CCCCCCCCEEECCCCCCHHHHHCCEEECCCCCCCCCCCHHHHHHHCCCCEEEEEECCCCE
EIDKDERRLSQVFARRVAETGLPLIFLNRIGGQDELVFDGCSFVLNGDGTAAHRLTDWEA
ECCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEECEEEEECCCCCCCCCCCCCHH
EERVTRWTKGAAGWACDAGAIAEWEAHPADIYSAMILSLRDYVTRNRFPGVVLGLSGGID
HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCH
SAICAAIAADALGPDKVWCVMLPSRFTSQASLDDAAGCARMIGCRLDTIPIVPAVEAFDQ
HHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHH
MLAGSFADAEVDITEENVQSRIRGVTLMALSNKFGPMLLTTGNKSEMSVGYATIYGDMAG
HHHCCCCCCEEEECHHHHHHHHCCEEEEEECCCCCCEEEECCCCCCCEEEEEEEEECCCC
GYNPLKDAYKMTVFALAKWRNEHVPRLSRNPVAPVMPDTIITKPPSAELRPDQKDQDSLP
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCC
PYEDLDRMLHMLVEEEAGVDDVVARHGFDRDAVARIERLLAIAEYKRRQAPPGVKLSTRN
CHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCC
FGRDRRYPITHGFRTG
CCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDAAATLTIVLSQMTQAVGDLAANADAMRSVRARHRDADLILYPELQLIGYPPEDLVLK
CCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEECCEEEECCCCHHHEEC
PALAERAATLLAELAADTADGGPAMLVGSVERDADGSLYNIVALLDGGRVVAVRRKHELP
HHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEEEEECCCEEEEEEECCCCC
NYGTFDEKRVFAPGPLPDIVEWRGVKLGLPICEDGWLPKVSAHLAAQGAELLISVNGSPY
CCCCCCCCEEECCCCCCHHHHHCCEEECCCCCCCCCCCHHHHHHHCCCCEEEEEECCCCE
EIDKDERRLSQVFARRVAETGLPLIFLNRIGGQDELVFDGCSFVLNGDGTAAHRLTDWEA
ECCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEECEEEEECCCCCCCCCCCCCHH
EERVTRWTKGAAGWACDAGAIAEWEAHPADIYSAMILSLRDYVTRNRFPGVVLGLSGGID
HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCH
SAICAAIAADALGPDKVWCVMLPSRFTSQASLDDAAGCARMIGCRLDTIPIVPAVEAFDQ
HHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHH
MLAGSFADAEVDITEENVQSRIRGVTLMALSNKFGPMLLTTGNKSEMSVGYATIYGDMAG
HHHCCCCCCEEEECHHHHHHHHCCEEEEEECCCCCCEEEECCCCCCCEEEEEEEEECCCC
GYNPLKDAYKMTVFALAKWRNEHVPRLSRNPVAPVMPDTIITKPPSAELRPDQKDQDSLP
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCC
PYEDLDRMLHMLVEEEAGVDDVVARHGFDRDAVARIERLLAIAEYKRRQAPPGVKLSTRN
CHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCC
FGRDRRYPITHGFRTG
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8431308; 8195100 [H]