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The map label for this gene is fliA [H]

Identifier: 103488379

GI number: 103488379

Start: 3052440

End: 3053186

Strand: Reverse

Name: fliA [H]

Synonym: Sala_2902

Alternate gene names: 103488379

Gene position: 3053186-3052440 (Counterclockwise)

Preceding gene: 103488380

Following gene: 103488371

Centisome position: 91.27

GC content: 66.53

Gene sequence:

>747_bases
ATGAGAATTGAGCCCGCCGAGCAATTTGCCGGGGCACCCGGCCGCGCGCTGCGCCCGCACGGATACGGCGAAAGCGTCGA
TGCGCTGGTGACGGAATATGCCCCGCTGGTGCGCAAGATCGCGTGGCAGGTCTTTTCGCGCGTGTCGCGAACGAGCGAGC
TCGACGACCTGATCCAGACCGGGCTGATCGCGCTGATCGAGGCGAGCCGCCATTATGAAGAGCGCGGCTTTGCCTTTGCC
ACCTATGCCTCGACGCGCATTCGCGGCGCGATGATCGACCAGCTGCGCCGCGAGGCGGACGTCGGGCGGTCGGCGATGGT
GGCGGCGAAACGGCTTCAGGCGACGCGCGCCGCGCTCGAACAGCAATTGCTGCGCGCGCCGACCGCGGCCGAAATGGCGG
TTGCGCTCGACATGGCGGCGGAAGATTATTTTGCGCTCGAGCGCAATGCGACGCACGGCCGCTCGACCTCGCTCGACGAA
CTCACCGACATCGGCGCCTTCCTCGCGGCCGACGAGGATGCGCTCGCCGACGACCGCTGCGAGCAGGAGGATCTGATGGG
CGCGCTGCGCCAGTGCGTCGGGCGGCTGTCCGAGCGCGAGCAGCTGGTGCTCCAGCTCTATTTCTTCGAGGAACTGAACC
TCCACGAAATCGGCCTGACGCTCGACGTCAGTGCGGCGCGCATCTGCCAGATCAAGCGCGAGGCGATGATCAAGGTCGAA
CGCATGATGCGCGAGATGACCGACTAG

Upstream 100 bases:

>100_bases
GAGATCCCGGCGACCAAGCAGACCGAAATCGTCGCGACGATCGGCGCCGACGTGCCGCGCCTCGCGACCGGACCCGATGC
CCCGATGGAGGATCATCTAA

Downstream 100 bases:

>100_bases
CGAGCCCCGAGGGATGACCGGGTGCAGCGCAGGGTTGCGCCGCACCCGGTCGGGGGGGGCAAGGCGAAGTATCAGGCGGC
CAGCGGTTGGCCGCCCCGGC

Product: RNA polymerase, sigma 28 subunit

Products: NA

Alternate protein names: Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MRIEPAEQFAGAPGRALRPHGYGESVDALVTEYAPLVRKIAWQVFSRVSRTSELDDLIQTGLIALIEASRHYEERGFAFA
TYASTRIRGAMIDQLRREADVGRSAMVAAKRLQATRAALEQQLLRAPTAAEMAVALDMAAEDYFALERNATHGRSTSLDE
LTDIGAFLAADEDALADDRCEQEDLMGALRQCVGRLSEREQLVLQLYFFEELNLHEIGLTLDVSAARICQIKREAMIKVE
RMMREMTD

Sequences:

>Translated_248_residues
MRIEPAEQFAGAPGRALRPHGYGESVDALVTEYAPLVRKIAWQVFSRVSRTSELDDLIQTGLIALIEASRHYEERGFAFA
TYASTRIRGAMIDQLRREADVGRSAMVAAKRLQATRAALEQQLLRAPTAAEMAVALDMAAEDYFALERNATHGRSTSLDE
LTDIGAFLAADEDALADDRCEQEDLMGALRQCVGRLSEREQLVLQLYFFEELNLHEIGLTLDVSAARICQIKREAMIKVE
RMMREMTD
>Mature_248_residues
MRIEPAEQFAGAPGRALRPHGYGESVDALVTEYAPLVRKIAWQVFSRVSRTSELDDLIQTGLIALIEASRHYEERGFAFA
TYASTRIRGAMIDQLRREADVGRSAMVAAKRLQATRAALEQQLLRAPTAAEMAVALDMAAEDYFALERNATHGRSTSLDE
LTDIGAFLAADEDALADDRCEQEDLMGALRQCVGRLSEREQLVLQLYFFEELNLHEIGLTLDVSAARICQIKREAMIKVE
RMMREMTD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for the flagellin gene (fliC) expression [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=218, Percent_Identity=37.1559633027523, Blast_Score=109, Evalue=2e-25,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27755; Mature: 27755

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIEPAEQFAGAPGRALRPHGYGESVDALVTEYAPLVRKIAWQVFSRVSRTSELDDLIQT
CCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLIALIEASRHYEERGFAFATYASTRIRGAMIDQLRREADVGRSAMVAAKRLQATRAALE
HHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
QQLLRAPTAAEMAVALDMAAEDYFALERNATHGRSTSLDELTDIGAFLAADEDALADDRC
HHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHCCCCC
EQEDLMGALRQCVGRLSEREQLVLQLYFFEELNLHEIGLTLDVSAARICQIKREAMIKVE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCEEEEHHHHHHHHHHHHHHHHHH
RMMREMTD
HHHHHHCC
>Mature Secondary Structure
MRIEPAEQFAGAPGRALRPHGYGESVDALVTEYAPLVRKIAWQVFSRVSRTSELDDLIQT
CCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLIALIEASRHYEERGFAFATYASTRIRGAMIDQLRREADVGRSAMVAAKRLQATRAALE
HHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
QQLLRAPTAAEMAVALDMAAEDYFALERNATHGRSTSLDELTDIGAFLAADEDALADDRC
HHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHCCCCC
EQEDLMGALRQCVGRLSEREQLVLQLYFFEELNLHEIGLTLDVSAARICQIKREAMIKVE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCEEEEHHHHHHHHHHHHHHHHHH
RMMREMTD
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1560774; 10984043 [H]