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The map label for this gene is mnmG

Identifier: 103488331

GI number: 103488331

Start: 3003081

End: 3004940

Strand: Direct

Name: mnmG

Synonym: Sala_2854

Alternate gene names: 103488331

Gene position: 3003081-3004940 (Clockwise)

Preceding gene: 103488330

Following gene: 103488332

Centisome position: 89.77

GC content: 68.82

Gene sequence:

>1860_bases
ATGCACAGCAGCGCAACATTCGATGTCATCGTCGTTGGCGGTGGACATGCCGGGACCGAGGCTGCCGCGGCCGCGGCGCG
GCTGGGCGCGCGGGTGGCGCTGGTCAGTTTCGACCCGACGCTGATCGGAACGATGTCGTGCAACCCGGCGATCGGCGGGC
TCGGCAAGGGCCATTTGATGCGCGAGGTCGACGCGCTCGACGGGTGGATGGCGCGCGCGGCCGACGCCGCCGCGATCCAT
TACCGGATGCTCAACGCATCGAAGGGTGCGGCGGTGCAGGGTCCGCGCATCCAGGCCGATCGCAAGCTCTATCGCGCCGC
GATCCAGACGCTGCTGGCAGCGGAGGAGGGGATCACGGTCGTTGCAGGCGAAGCGGCGGCTCTGCGACTATCCAACGGGG
GGCGGATCGCAGGACTCGATCTCGCCGACGGGACGGCGCTCACGGCGCGCGCGGTTGTGCTCGCGACGGGTACTTTCCTC
GGCGGACGACTGTTCCGCGGCGAAGAACGGATGGAGGGTGGCCGGATCGGCGAAGCGGGGTCGCACCGTCTCGCCGGGCA
GCTTCGCGCCGCCCACCTGCCGATGGCGCGGCTCAAGACGGGCACGCCGCCGCGCCTCGATGGCCGCACGATCGACTGGG
CGCGGTTGCAGGAACAGCCGTCGGATGGCGGACGCTGGACCTGTTCCACGTGGAACAACGCGCGGACGGTGCCGCAGATT
TTCTGCGCAATCACGCGCACCAACGCCGAATCGCACCGGATCATCGCCGAAAACCTGCATCGCTCGCCGCTCTTTACCGG
CGCGATCGGTGCGGCGGGGCCGCGTTATTGCCCGTCGATCGAGGACAAGATCCACCGCTTTGCCGATCGCGACGGGCATC
AGGTGTTTCTGGAGCCCGAGGGGCTTGATACGCATCTGGTCTATCCCAATGGCATTTCGACTTCGCTGCCCGCCGATGTG
CAACTGGCGATGCTGCGCACGATCGAGGGGCTGGAGGCGGTCGAGATGGTCGTGCCCGGCTATGCGGTCGAATATGACCA
TATCGACCCGCGTGCGCTCGACCGGACGTTGCAGGTGCGCGCGATGCCGGGGGTCTGGTGTGCGGGACAGATCAACGGCA
CGACGGGATATGAGGAGGCCGCGGCGCAGGGGCTGGTCGCGGGCGCCAATGCCGCGCTCGCCGTGCAGGGGCGCGAGCCG
CTGATCCTCGACCGGAGCGAATCCTATATCGGGGTGATGGTCGACGACCTGGTGTTGCAGGGCGTGACCGAGCCGTACCG
GATGCTCACCGCGCGCGCCGAATATCGGCTGCGGCTGCGCGCCGACAATGCGGCGACCCGGCTGACGCCGAAGGGGATTG
CACTGGGGCTGGTGCGGCCGGCGACCGCGGCGCTGTTTGCCAGGAGGATGGCCGAGCGGGCGCGGGCCGGGGCGCTGCTC
GAAGCGCCGGTGGCGACCGCCGATTATGGGGCGATCGGCTTGCCGCTGCCCGGCGACGGGATCGCGCGGCGGCGAATCGA
CCTGTTGCGCTTTCCCGGTGCGACGGTCGAGCGGTTGACGATTCTGGTTCCCGAGATCGAATCGATCGATCGCGCGATCC
TGTCCGAAGTCGTCGAAGATGCGCATTATGCGCCCTATATCGCGCGGCACGAAGCCGAGCTGCGCGCGCTCGCAGCAAAT
GAGGCGATCCTGCTCGATCCCGCGCTCGACTATGCCGCGATCGGCGGCCTGTCGCGCGAAATGGTCGAGCGGCTGAGCAA
GGCACGCCCCGAAACGCTGGGACAGGCGGCGCGAATTGATGGCGTGACGCCCGCGGCGCTGACGGCGATCATGGTGCATA
GCCGGCGGCGCGCGGCCTGA

Upstream 100 bases:

>100_bases
TGGGCGATTCTGCATCGGCAAATGACGTTCCACGTGAAACATCGTGCAGATCGCAGCGCAGTGGATTCGCCGTCGGCTTT
ACGCTAAGGGCGCCCCGATC

Downstream 100 bases:

>100_bases
ATCGGCGAAGACAGGTTCTGGAATCTCACGCAAAGGCGCAAAGGCGCGAAGAAGGATGATAATCCCGCATCGTGCGGATG
GTGGAAAGAACGATGTGAGC

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 619; Mature: 619

Protein sequence:

>619_residues
MHSSATFDVIVVGGGHAGTEAAAAAARLGARVALVSFDPTLIGTMSCNPAIGGLGKGHLMREVDALDGWMARAADAAAIH
YRMLNASKGAAVQGPRIQADRKLYRAAIQTLLAAEEGITVVAGEAAALRLSNGGRIAGLDLADGTALTARAVVLATGTFL
GGRLFRGEERMEGGRIGEAGSHRLAGQLRAAHLPMARLKTGTPPRLDGRTIDWARLQEQPSDGGRWTCSTWNNARTVPQI
FCAITRTNAESHRIIAENLHRSPLFTGAIGAAGPRYCPSIEDKIHRFADRDGHQVFLEPEGLDTHLVYPNGISTSLPADV
QLAMLRTIEGLEAVEMVVPGYAVEYDHIDPRALDRTLQVRAMPGVWCAGQINGTTGYEEAAAQGLVAGANAALAVQGREP
LILDRSESYIGVMVDDLVLQGVTEPYRMLTARAEYRLRLRADNAATRLTPKGIALGLVRPATAALFARRMAERARAGALL
EAPVATADYGAIGLPLPGDGIARRRIDLLRFPGATVERLTILVPEIESIDRAILSEVVEDAHYAPYIARHEAELRALAAN
EAILLDPALDYAAIGGLSREMVERLSKARPETLGQAARIDGVTPAALTAIMVHSRRRAA

Sequences:

>Translated_619_residues
MHSSATFDVIVVGGGHAGTEAAAAAARLGARVALVSFDPTLIGTMSCNPAIGGLGKGHLMREVDALDGWMARAADAAAIH
YRMLNASKGAAVQGPRIQADRKLYRAAIQTLLAAEEGITVVAGEAAALRLSNGGRIAGLDLADGTALTARAVVLATGTFL
GGRLFRGEERMEGGRIGEAGSHRLAGQLRAAHLPMARLKTGTPPRLDGRTIDWARLQEQPSDGGRWTCSTWNNARTVPQI
FCAITRTNAESHRIIAENLHRSPLFTGAIGAAGPRYCPSIEDKIHRFADRDGHQVFLEPEGLDTHLVYPNGISTSLPADV
QLAMLRTIEGLEAVEMVVPGYAVEYDHIDPRALDRTLQVRAMPGVWCAGQINGTTGYEEAAAQGLVAGANAALAVQGREP
LILDRSESYIGVMVDDLVLQGVTEPYRMLTARAEYRLRLRADNAATRLTPKGIALGLVRPATAALFARRMAERARAGALL
EAPVATADYGAIGLPLPGDGIARRRIDLLRFPGATVERLTILVPEIESIDRAILSEVVEDAHYAPYIARHEAELRALAAN
EAILLDPALDYAAIGGLSREMVERLSKARPETLGQAARIDGVTPAALTAIMVHSRRRAA
>Mature_619_residues
MHSSATFDVIVVGGGHAGTEAAAAAARLGARVALVSFDPTLIGTMSCNPAIGGLGKGHLMREVDALDGWMARAADAAAIH
YRMLNASKGAAVQGPRIQADRKLYRAAIQTLLAAEEGITVVAGEAAALRLSNGGRIAGLDLADGTALTARAVVLATGTFL
GGRLFRGEERMEGGRIGEAGSHRLAGQLRAAHLPMARLKTGTPPRLDGRTIDWARLQEQPSDGGRWTCSTWNNARTVPQI
FCAITRTNAESHRIIAENLHRSPLFTGAIGAAGPRYCPSIEDKIHRFADRDGHQVFLEPEGLDTHLVYPNGISTSLPADV
QLAMLRTIEGLEAVEMVVPGYAVEYDHIDPRALDRTLQVRAMPGVWCAGQINGTTGYEEAAAQGLVAGANAALAVQGREP
LILDRSESYIGVMVDDLVLQGVTEPYRMLTARAEYRLRLRADNAATRLTPKGIALGLVRPATAALFARRMAERARAGALL
EAPVATADYGAIGLPLPGDGIARRRIDLLRFPGATVERLTILVPEIESIDRAILSEVVEDAHYAPYIARHEAELRALAAN
EAILLDPALDYAAIGGLSREMVERLSKARPETLGQAARIDGVTPAALTAIMVHSRRRAA

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=632, Percent_Identity=40.6645569620253, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI19882217, Length=657, Percent_Identity=39.117199391172, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI183227703, Length=672, Percent_Identity=38.2440476190476, Blast_Score=410, Evalue=1e-114,
Organism=Escherichia coli, GI2367273, Length=626, Percent_Identity=47.7635782747604, Blast_Score=535, Evalue=1e-153,
Organism=Caenorhabditis elegans, GI17534255, Length=616, Percent_Identity=39.9350649350649, Blast_Score=423, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6321202, Length=628, Percent_Identity=41.2420382165605, Blast_Score=471, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24658174, Length=629, Percent_Identity=42.4483306836248, Blast_Score=430, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_SPHAL (Q1GP63)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_617892.1
- ProteinModelPortal:   Q1GP63
- SMR:   Q1GP63
- GeneID:   4080647
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_2854
- NMPDR:   fig|317655.9.peg.2717
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- ProtClustDB:   PRK05192
- BioCyc:   SALA317655:SALA_2854-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 65873; Mature: 65873

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSSATFDVIVVGGGHAGTEAAAAAARLGARVALVSFDPTLIGTMSCNPAIGGLGKGHLM
CCCCCEEEEEEEECCCCCHHHHHHHHHHCCEEEEEEECCEEEEEECCCCCCCCCCCCHHH
REVDALDGWMARAADAAAIHYRMLNASKGAAVQGPRIQADRKLYRAAIQTLLAAEEGITV
HHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEE
VAGEAAALRLSNGGRIAGLDLADGTALTARAVVLATGTFLGGRLFRGEERMEGGRIGEAG
EECCEEEEEECCCCEEEEEECCCCCHHHHEEEEEEECHHHCCEEECCHHHCCCCCCCCCC
SHRLAGQLRAAHLPMARLKTGTPPRLDGRTIDWARLQEQPSDGGRWTCSTWNNARTVPQI
CCHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCHHHHHCCCCCCCCEEEECCCCCCHHHHH
FCAITRTNAESHRIIAENLHRSPLFTGAIGAAGPRYCPSIEDKIHRFADRDGHQVFLEPE
HEEHEECCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECC
GLDTHLVYPNGISTSLPADVQLAMLRTIEGLEAVEMVVPGYAVEYDHIDPRALDRTLQVR
CCCEEEECCCCCCCCCCCHHHHHHHHHHCCHHHHHHHCCCEEEEECCCCHHHHCCEEEEE
AMPGVWCAGQINGTTGYEEAAAQGLVAGANAALAVQGREPLILDRSESYIGVMVDDLVLQ
ECCCEEEEEEECCCCCHHHHHHCCEEECCCEEEEECCCCCEEEECCCCEEEHHHHHHHHH
GVTEPYRMLTARAEYRLRLRADNAATRLTPKGIALGLVRPATAALFARRMAERARAGALL
CCCHHHHHHHHCCEEEEEEEECCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEE
EAPVATADYGAIGLPLPGDGIARRRIDLLRFPGATVERLTILVPEIESIDRAILSEVVED
ECCCCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHH
AHYAPYIARHEAELRALAANEAILLDPALDYAAIGGLSREMVERLSKARPETLGQAARID
CCCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHCCCHHHHHHHHHHCCCHHHCHHHHCC
GVTPAALTAIMVHSRRRAA
CCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHSSATFDVIVVGGGHAGTEAAAAAARLGARVALVSFDPTLIGTMSCNPAIGGLGKGHLM
CCCCCEEEEEEEECCCCCHHHHHHHHHHCCEEEEEEECCEEEEEECCCCCCCCCCCCHHH
REVDALDGWMARAADAAAIHYRMLNASKGAAVQGPRIQADRKLYRAAIQTLLAAEEGITV
HHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEE
VAGEAAALRLSNGGRIAGLDLADGTALTARAVVLATGTFLGGRLFRGEERMEGGRIGEAG
EECCEEEEEECCCCEEEEEECCCCCHHHHEEEEEEECHHHCCEEECCHHHCCCCCCCCCC
SHRLAGQLRAAHLPMARLKTGTPPRLDGRTIDWARLQEQPSDGGRWTCSTWNNARTVPQI
CCHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCHHHHHCCCCCCCCEEEECCCCCCHHHHH
FCAITRTNAESHRIIAENLHRSPLFTGAIGAAGPRYCPSIEDKIHRFADRDGHQVFLEPE
HEEHEECCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECC
GLDTHLVYPNGISTSLPADVQLAMLRTIEGLEAVEMVVPGYAVEYDHIDPRALDRTLQVR
CCCEEEECCCCCCCCCCCHHHHHHHHHHCCHHHHHHHCCCEEEEECCCCHHHHCCEEEEE
AMPGVWCAGQINGTTGYEEAAAQGLVAGANAALAVQGREPLILDRSESYIGVMVDDLVLQ
ECCCEEEEEEECCCCCHHHHHHCCEEECCCEEEEECCCCCEEEECCCCEEEHHHHHHHHH
GVTEPYRMLTARAEYRLRLRADNAATRLTPKGIALGLVRPATAALFARRMAERARAGALL
CCCHHHHHHHHCCEEEEEEEECCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEE
EAPVATADYGAIGLPLPGDGIARRRIDLLRFPGATVERLTILVPEIESIDRAILSEVVED
ECCCCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHH
AHYAPYIARHEAELRALAANEAILLDPALDYAAIGGLSREMVERLSKARPETLGQAARID
CCCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHCCCHHHHHHHHHHCCCHHHCHHHHCC
GVTPAALTAIMVHSRRRAA
CCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA