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The map label for this gene is trmE

Identifier: 103488330

GI number: 103488330

Start: 3001746

End: 3003005

Strand: Direct

Name: trmE

Synonym: Sala_2853

Alternate gene names: 103488330

Gene position: 3001746-3003005 (Clockwise)

Preceding gene: 103488329

Following gene: 103488331

Centisome position: 89.73

GC content: 68.89

Gene sequence:

>1260_bases
GTGCGCATCAGCGGCATCGATGCCGCCAAGGCTTTACAGGCACTGGCGGGGCGCCTCCCCACACCCCGCCGCGCATCGCT
CGCCCGGTTGACGGACACCGACGGCGGGGCGCTCGATCATGCGCTCATCCTCTGGTTTCCGGGGCCAGCGACCGCGACCG
GCGAAGACCTCGCCGAACTGCACCTGCACGGCGGACGCGCGGTGGTTGCGGCGGTCGAAGCGGCGCTGGCGGCGATGCCG
GGACTGCGGCGTGCCGAAGCGGGTGAGTTTACGCGGCGGGCGTTTGCGAATGGGCGGATCGATCTGGCCGAGGCCGAGGG
GCTCGCCGACCTGCTCGCCGCCGAAACCGAGAGCCAGCGTGTGCAGGCGCTGGATCATGCGAGCGGGCATGTGTCGCGCG
CGGTGGCGGGGTGGCAGGCGCGGCTACTCGCGCTGATGGCGGCGGCCGAGGCCGAACTTAACTTCGCGGATGAGGATGAC
GTCGAAGTTGGCGAAGGTGTCGCGCAGCGCGTGTCGGAGGGGATGGCGGCCCTGGCGGGGGAGCTCGGCGAGTGGCTGGC
GCGGCCGGCGGCGGAGGTGATTGCCGAGGGGCTGTCGGTGGTGATCGCTGGGCCGCCGAACGCAGGTAAATCTACTCTTA
TCAATGCTCTAGCTCAGAGGGAGCTGGCGATCGTGTCGCCGGTCGCCGGGACGACGCGCGATGTGATCGAAACGCCGCTC
GCGCTGGACGGAATTGCAATGCGCTTTTCGGATACTGCCGGCCTGCGCGGCGAAAGTGCCGACGCGATCGAAATGATTGG
AATCGATCGCGCGAAGGCGGCAGTGGAGGGTGCGGACATCCTGTTGTGGTTGGGCGCGCCGAAGGAGGCGCCCGAGCATC
CTCGCACGATCCTGATCGCCGCGCAGGCCGACCGCTGGCGCGGCGATGCCGCGGCCGAAGCCGAGGCCGCGCACTGCGAT
CTTATATTGTCCGCCGCCACGGGGGAGGGGATGGACCGGTTGCACACGATGATCGTCGAGATGGCGCGAACCTTATTGCC
GCGCGAGGGCGAGGCTACGCTCCGGCAGCGACAGCGTGATGCGCTCGCCGAGGCAAAGGGGTGGCTGGAAGTTGAGACGG
GATCGCGCGAGGCGGGCGATCTGATCCTGCTGGCCGAGCGGCTGCGGCTTGCCGGGGCCACGCTGGACCGTATCACCGGG
CGCGGGGGCGTTGAAGATATGCTCGACACGCTGTTTGGGCGATTCTGCATCGGCAAATGA

Upstream 100 bases:

>100_bases
ACGACAAGCCCGCCAAGGGACGCAAGAGCTGAACCAAACACCCCCAAGCGACACGATTTTCGCGCTATCGAGCGGGATAC
CGCCGGCGGCGATTGCGGTC

Downstream 100 bases:

>100_bases
CGTTCCACGTGAAACATCGTGCAGATCGCAGCGCAGTGGATTCGCCGTCGGCTTTACGCTAAGGGCGCCCCGATCATGCA
CAGCAGCGCAACATTCGATG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MRISGIDAAKALQALAGRLPTPRRASLARLTDTDGGALDHALILWFPGPATATGEDLAELHLHGGRAVVAAVEAALAAMP
GLRRAEAGEFTRRAFANGRIDLAEAEGLADLLAAETESQRVQALDHASGHVSRAVAGWQARLLALMAAAEAELNFADEDD
VEVGEGVAQRVSEGMAALAGELGEWLARPAAEVIAEGLSVVIAGPPNAGKSTLINALAQRELAIVSPVAGTTRDVIETPL
ALDGIAMRFSDTAGLRGESADAIEMIGIDRAKAAVEGADILLWLGAPKEAPEHPRTILIAAQADRWRGDAAAEAEAAHCD
LILSAATGEGMDRLHTMIVEMARTLLPREGEATLRQRQRDALAEAKGWLEVETGSREAGDLILLAERLRLAGATLDRITG
RGGVEDMLDTLFGRFCIGK

Sequences:

>Translated_419_residues
MRISGIDAAKALQALAGRLPTPRRASLARLTDTDGGALDHALILWFPGPATATGEDLAELHLHGGRAVVAAVEAALAAMP
GLRRAEAGEFTRRAFANGRIDLAEAEGLADLLAAETESQRVQALDHASGHVSRAVAGWQARLLALMAAAEAELNFADEDD
VEVGEGVAQRVSEGMAALAGELGEWLARPAAEVIAEGLSVVIAGPPNAGKSTLINALAQRELAIVSPVAGTTRDVIETPL
ALDGIAMRFSDTAGLRGESADAIEMIGIDRAKAAVEGADILLWLGAPKEAPEHPRTILIAAQADRWRGDAAAEAEAAHCD
LILSAATGEGMDRLHTMIVEMARTLLPREGEATLRQRQRDALAEAKGWLEVETGSREAGDLILLAERLRLAGATLDRITG
RGGVEDMLDTLFGRFCIGK
>Mature_419_residues
MRISGIDAAKALQALAGRLPTPRRASLARLTDTDGGALDHALILWFPGPATATGEDLAELHLHGGRAVVAAVEAALAAMP
GLRRAEAGEFTRRAFANGRIDLAEAEGLADLLAAETESQRVQALDHASGHVSRAVAGWQARLLALMAAAEAELNFADEDD
VEVGEGVAQRVSEGMAALAGELGEWLARPAAEVIAEGLSVVIAGPPNAGKSTLINALAQRELAIVSPVAGTTRDVIETPL
ALDGIAMRFSDTAGLRGESADAIEMIGIDRAKAAVEGADILLWLGAPKEAPEHPRTILIAAQADRWRGDAAAEAEAAHCD
LILSAATGEGMDRLHTMIVEMARTLLPREGEATLRQRQRDALAEAKGWLEVETGSREAGDLILLAERLRLAGATLDRITG
RGGVEDMLDTLFGRFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082993, Length=426, Percent_Identity=42.2535211267606, Blast_Score=278, Evalue=8e-75,
Organism=Homo sapiens, GI193082991, Length=447, Percent_Identity=40.9395973154362, Blast_Score=275, Evalue=4e-74,
Organism=Homo sapiens, GI193082988, Length=479, Percent_Identity=38.6221294363257, Blast_Score=259, Evalue=4e-69,
Organism=Escherichia coli, GI2367268, Length=443, Percent_Identity=38.8261851015801, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI87082120, Length=89, Percent_Identity=37.0786516853933, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17507259, Length=435, Percent_Identity=31.7241379310345, Blast_Score=197, Evalue=9e-51,
Organism=Saccharomyces cerevisiae, GI6323665, Length=472, Percent_Identity=30.2966101694915, Blast_Score=228, Evalue=1e-60,
Organism=Drosophila melanogaster, GI45550813, Length=457, Percent_Identity=34.7921225382932, Blast_Score=243, Evalue=2e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_SPHAL (Q1GP64)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_617891.1
- ProteinModelPortal:   Q1GP64
- SMR:   Q1GP64
- GeneID:   4080646
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_2853
- NMPDR:   fig|317655.9.peg.2716
- HOGENOM:   HBG746795
- OMA:   LIDNAMV
- ProtClustDB:   PRK05291
- BioCyc:   SALA317655:SALA_2853-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR003593
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- SMART:   SM00382
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 43906; Mature: 43906

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: BINDING 2-2 BINDING 59-59 BINDING 99-99 BINDING 419-419

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRISGIDAAKALQALAGRLPTPRRASLARLTDTDGGALDHALILWFPGPATATGEDLAEL
CCCCCCCHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHH
HLHGGRAVVAAVEAALAAMPGLRRAEAGEFTRRAFANGRIDLAEAEGLADLLAAETESQR
HHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEHHHCCHHHHHHHCCHHHH
VQALDHASGHVSRAVAGWQARLLALMAAAEAELNFADEDDVEVGEGVAQRVSEGMAALAG
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ELGEWLARPAAEVIAEGLSVVIAGPPNAGKSTLINALAQRELAIVSPVAGTTRDVIETPL
HHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHEECCCCCCHHHHHHCCC
ALDGIAMRFSDTAGLRGESADAIEMIGIDRAKAAVEGADILLWLGAPKEAPEHPRTILIA
HHCCCCEEECCCCCCCCCCCCHHHHHCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEE
AQADRWRGDAAAEAEAAHCDLILSAATGEGMDRLHTMIVEMARTLLPREGEATLRQRQRD
ECCCCCCCCCCCCCHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
ALAEAKGWLEVETGSREAGDLILLAERLRLAGATLDRITGRGGVEDMLDTLFGRFCIGK
HHHHHCCCEEEECCCCCCCCCHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRISGIDAAKALQALAGRLPTPRRASLARLTDTDGGALDHALILWFPGPATATGEDLAEL
CCCCCCCHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHH
HLHGGRAVVAAVEAALAAMPGLRRAEAGEFTRRAFANGRIDLAEAEGLADLLAAETESQR
HHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEHHHCCHHHHHHHCCHHHH
VQALDHASGHVSRAVAGWQARLLALMAAAEAELNFADEDDVEVGEGVAQRVSEGMAALAG
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ELGEWLARPAAEVIAEGLSVVIAGPPNAGKSTLINALAQRELAIVSPVAGTTRDVIETPL
HHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHEECCCCCCHHHHHHCCC
ALDGIAMRFSDTAGLRGESADAIEMIGIDRAKAAVEGADILLWLGAPKEAPEHPRTILIA
HHCCCCEEECCCCCCCCCCCCHHHHHCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEE
AQADRWRGDAAAEAEAAHCDLILSAATGEGMDRLHTMIVEMARTLLPREGEATLRQRQRD
ECCCCCCCCCCCCCHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
ALAEAKGWLEVETGSREAGDLILLAERLRLAGATLDRITGRGGVEDMLDTLFGRFCIGK
HHHHHCCCEEEECCCCCCCCCHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA