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The map label for this gene is merA [H]

Identifier: 103487991

GI number: 103487991

Start: 2647410

End: 2648849

Strand: Direct

Name: merA [H]

Synonym: Sala_2512

Alternate gene names: 103487991

Gene position: 2647410-2648849 (Clockwise)

Preceding gene: 103487990

Following gene: 103487992

Centisome position: 79.14

GC content: 63.82

Gene sequence:

>1440_bases
ATGAGTGATTGCTGCAATCGGGGCGACGGCTCTCAGGCCAAGAAGTACGACCTTATCGTCGTGGGCGGCGGCTCGGCTGG
GTTCTCGGCCGCGATTACCGCGGCGGAGCAAGGAGCCCAAGTCGCCGTTATCGGTGCCGGCACCATCGGCGGAACCTGCG
TCAACGTCGGCTGCGTTCCTTCCAAGGCGCTGATCCGCGCGGTCGAGAGCATTCACCACGCGAATGCCGCGCCGATGCGG
TTCAACGGTGTCGAGGCCGGGGCACGAATGGCCGATTGGGGCAAGGTGATCGCCGAAAAGGACTCGCTCGTCTCGGGCCT
TCGTCAGGCGAAATATGCGGATCTCCTGCCTCTCTACAATAATATCGCCTACCATGAAGGCACGGCGCGGCTCGTCGAAA
ATGGCGTCGAGGCCGGTGGAAGGCGGTTCACCGCTGACCGGATCGTGATTGCGACAGGTACGCGTCCGGCGGTGCCGGCT
ATCCCGGGCCTTCCGGACGTCGATGCGCTCGACAGCACTACCGCGCTCGACCTGACCGAGTTGCCGAAATCGATGATCGT
CCTCGGGGGCGGCTATATCGGTGTGGAACTCGCCCAGATGTTCTCCCGCGCCGGCGTCGACGTGACGCTCGTTTTCCGCA
GCCGCCTTCTCCCCGATATGGAGCCGGAGATCGGTGCCGCGCTGACCGACTATCTTTCGAGCGAAGGCATCACGGTTCTG
GGCGACCTCGCCTATCAGTCGGCCCACAAGACGGCGGAAGGGGGCACTGCGCTCACCGTCCTGCGCAACGGGGTCGCCGA
AACCATCGTCGCGGAGCGCCTTCTCGTAGCGACCGGGCGCGCCCCGAATGTGGAGGACCTCGGTCTCATCGAGGCAGGGG
TGAAGCAGACGCTCAGCGGTGCCATCATTGTCGATGACCATATGCGAACCTCGGTGCGCGGCGTCTATGCCGCCGGCGAT
GTGACGGGCCGAGACCAGTTCGTTTACATGGCAGCCTATGGCGCAAAGATCGCCGCCAAGAACGCCTTGAACGGCGATAG
CCTGCGGTACGACAATTCGGCCATGCCGGCCGTCGTATTCAGCGATCCGCAGGTAGCCAGTGTCGGCTTCACCGAAGCGC
AGGCTATCGCGGCAGGATATGCAACGCGAACCTCGACCCTCCCGCTCGAGAATGTTCCGCGCGCATTGGCGGCTCGCGAC
ACGCGCGGCCTGATCAAGCTGGTTGCGGATGGCCGGACCCGCAAACTGCTGGGCGCGCATATCTTGGCGCCGGAAGGTGC
GGACAGTATCCAGACCGCCGCCATGGCGATCCGCTGCGGCCTCACGATCGACGATCTGGCGGAGATGATCTTCCCCTATC
TGACCACGGTCGAAGGACTGAAGCTGGCTGCGCAGACATTCGATCGGGATGTGAAAAGGCTGTCCTGCTGTGCAGGATAA

Upstream 100 bases:

>100_bases
ACGGTCACCTATGACGACGCCAAGACCAGCACCGCGGCGTTGATCGCGGCGACCACCAACGCTGGCTACCCTTCCCGAGT
CCGCAAGTGAAAGTCGTATC

Downstream 100 bases:

>100_bases
GATTGCTCGGTCGCGTCGCGGCGGTGAGATGACATGAGTAGCCCGCGCGGTTCCGATCCCGCCAGCGGCGCCGGGGATTG
GTCGGGTAACTGGCGCACGC

Product: mercuric reductase MerA

Products: NA

Alternate protein names: Hg(II) reductase [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MSDCCNRGDGSQAKKYDLIVVGGGSAGFSAAITAAEQGAQVAVIGAGTIGGTCVNVGCVPSKALIRAVESIHHANAAPMR
FNGVEAGARMADWGKVIAEKDSLVSGLRQAKYADLLPLYNNIAYHEGTARLVENGVEAGGRRFTADRIVIATGTRPAVPA
IPGLPDVDALDSTTALDLTELPKSMIVLGGGYIGVELAQMFSRAGVDVTLVFRSRLLPDMEPEIGAALTDYLSSEGITVL
GDLAYQSAHKTAEGGTALTVLRNGVAETIVAERLLVATGRAPNVEDLGLIEAGVKQTLSGAIIVDDHMRTSVRGVYAAGD
VTGRDQFVYMAAYGAKIAAKNALNGDSLRYDNSAMPAVVFSDPQVASVGFTEAQAIAAGYATRTSTLPLENVPRALAARD
TRGLIKLVADGRTRKLLGAHILAPEGADSIQTAAMAIRCGLTIDDLAEMIFPYLTTVEGLKLAAQTFDRDVKRLSCCAG

Sequences:

>Translated_479_residues
MSDCCNRGDGSQAKKYDLIVVGGGSAGFSAAITAAEQGAQVAVIGAGTIGGTCVNVGCVPSKALIRAVESIHHANAAPMR
FNGVEAGARMADWGKVIAEKDSLVSGLRQAKYADLLPLYNNIAYHEGTARLVENGVEAGGRRFTADRIVIATGTRPAVPA
IPGLPDVDALDSTTALDLTELPKSMIVLGGGYIGVELAQMFSRAGVDVTLVFRSRLLPDMEPEIGAALTDYLSSEGITVL
GDLAYQSAHKTAEGGTALTVLRNGVAETIVAERLLVATGRAPNVEDLGLIEAGVKQTLSGAIIVDDHMRTSVRGVYAAGD
VTGRDQFVYMAAYGAKIAAKNALNGDSLRYDNSAMPAVVFSDPQVASVGFTEAQAIAAGYATRTSTLPLENVPRALAARD
TRGLIKLVADGRTRKLLGAHILAPEGADSIQTAAMAIRCGLTIDDLAEMIFPYLTTVEGLKLAAQTFDRDVKRLSCCAG
>Mature_478_residues
SDCCNRGDGSQAKKYDLIVVGGGSAGFSAAITAAEQGAQVAVIGAGTIGGTCVNVGCVPSKALIRAVESIHHANAAPMRF
NGVEAGARMADWGKVIAEKDSLVSGLRQAKYADLLPLYNNIAYHEGTARLVENGVEAGGRRFTADRIVIATGTRPAVPAI
PGLPDVDALDSTTALDLTELPKSMIVLGGGYIGVELAQMFSRAGVDVTLVFRSRLLPDMEPEIGAALTDYLSSEGITVLG
DLAYQSAHKTAEGGTALTVLRNGVAETIVAERLLVATGRAPNVEDLGLIEAGVKQTLSGAIIVDDHMRTSVRGVYAAGDV
TGRDQFVYMAAYGAKIAAKNALNGDSLRYDNSAMPAVVFSDPQVASVGFTEAQAIAAGYATRTSTLPLENVPRALAARDT
RGLIKLVADGRTRKLLGAHILAPEGADSIQTAAMAIRCGLTIDDLAEMIFPYLTTVEGLKLAAQTFDRDVKRLSCCAG

Specific function: Resistance to Hg(2+) in bacteria appears to be governed by a specialized system which includes mercuric reductase. MerA protein is responsible for volatilizing mercury as Hg(0) [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=470, Percent_Identity=30.8510638297872, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI50301238, Length=457, Percent_Identity=31.9474835886214, Blast_Score=182, Evalue=6e-46,
Organism=Homo sapiens, GI22035672, Length=454, Percent_Identity=29.9559471365639, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI33519430, Length=447, Percent_Identity=25.0559284116331, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI33519428, Length=447, Percent_Identity=25.0559284116331, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI33519426, Length=447, Percent_Identity=25.0559284116331, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI148277071, Length=447, Percent_Identity=25.0559284116331, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI148277065, Length=447, Percent_Identity=25.0559284116331, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI291045266, Length=422, Percent_Identity=27.0142180094787, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI291045268, Length=416, Percent_Identity=25.9615384615385, Blast_Score=99, Evalue=8e-21,
Organism=Escherichia coli, GI1786307, Length=469, Percent_Identity=30.2771855010661, Blast_Score=194, Evalue=9e-51,
Organism=Escherichia coli, GI87081717, Length=459, Percent_Identity=31.1546840958606, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1789915, Length=454, Percent_Identity=32.15859030837, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI87082354, Length=467, Percent_Identity=26.9807280513919, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1789065, Length=223, Percent_Identity=30.9417040358744, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI32565766, Length=471, Percent_Identity=30.7855626326964, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17557007, Length=498, Percent_Identity=27.710843373494, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI71983429, Length=460, Percent_Identity=30.2173913043478, Blast_Score=151, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI71983419, Length=460, Percent_Identity=30.2173913043478, Blast_Score=151, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI71982272, Length=501, Percent_Identity=25.9481037924152, Blast_Score=115, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6321091, Length=477, Percent_Identity=30.8176100628931, Blast_Score=187, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6325166, Length=473, Percent_Identity=29.1754756871036, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6325240, Length=490, Percent_Identity=27.5510204081633, Blast_Score=118, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21358499, Length=466, Percent_Identity=30.68669527897, Blast_Score=192, Evalue=4e-49,
Organism=Drosophila melanogaster, GI17737741, Length=492, Percent_Identity=29.4715447154472, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24640553, Length=493, Percent_Identity=29.0060851926978, Blast_Score=152, Evalue=6e-37,
Organism=Drosophila melanogaster, GI24640549, Length=493, Percent_Identity=29.0060851926978, Blast_Score=151, Evalue=8e-37,
Organism=Drosophila melanogaster, GI24640551, Length=494, Percent_Identity=29.3522267206478, Blast_Score=151, Evalue=8e-37,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000815
- InterPro:   IPR021179
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00403 HMA; PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.16.1.1 [H]

Molecular weight: Translated: 49838; Mature: 49707

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDCCNRGDGSQAKKYDLIVVGGGSAGFSAAITAAEQGAQVAVIGAGTIGGTCVNVGCVP
CCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHCCCCEEEEEECCCCCCEEEEECCCC
SKALIRAVESIHHANAAPMRFNGVEAGARMADWGKVIAEKDSLVSGLRQAKYADLLPLYN
HHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIAYHEGTARLVENGVEAGGRRFTADRIVIATGTRPAVPAIPGLPDVDALDSTTALDLTE
CHHHCCCHHHHHHHHHHCCCCEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHH
LPKSMIVLGGGYIGVELAQMFSRAGVDVTLVFRSRLLPDMEPEIGAALTDYLSSEGITVL
CCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCHHHHHHHHHHCCCCCEEE
GDLAYQSAHKTAEGGTALTVLRNGVAETIVAERLLVATGRAPNVEDLGLIEAGVKQTLSG
EHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCC
AIIVDDHMRTSVRGVYAAGDVTGRDQFVYMAAYGAKIAAKNALNGDSLRYDNSAMPAVVF
EEEEECCHHHHHCEEEEECCCCCCCCEEEEEECCCHHHHCCCCCCCCEEECCCCCCEEEE
SDPQVASVGFTEAQAIAAGYATRTSTLPLENVPRALAARDTRGLIKLVADGRTRKLLGAH
CCCCEEECCCHHHHHHHHCCCCCCCCCCHHHCCHHHHHCCCCCCEEEECCCCHHHHHHHE
ILAPEGADSIQTAAMAIRCGLTIDDLAEMIFPYLTTVEGLKLAAQTFDRDVKRLSCCAG
EECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDCCNRGDGSQAKKYDLIVVGGGSAGFSAAITAAEQGAQVAVIGAGTIGGTCVNVGCVP
CCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHCCCCEEEEEECCCCCCEEEEECCCC
SKALIRAVESIHHANAAPMRFNGVEAGARMADWGKVIAEKDSLVSGLRQAKYADLLPLYN
HHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIAYHEGTARLVENGVEAGGRRFTADRIVIATGTRPAVPAIPGLPDVDALDSTTALDLTE
CHHHCCCHHHHHHHHHHCCCCEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHH
LPKSMIVLGGGYIGVELAQMFSRAGVDVTLVFRSRLLPDMEPEIGAALTDYLSSEGITVL
CCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCHHHHHHHHHHCCCCCEEE
GDLAYQSAHKTAEGGTALTVLRNGVAETIVAERLLVATGRAPNVEDLGLIEAGVKQTLSG
EHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCC
AIIVDDHMRTSVRGVYAAGDVTGRDQFVYMAAYGAKIAAKNALNGDSLRYDNSAMPAVVF
EEEEECCHHHHHCEEEEECCCCCCCCEEEEEECCCHHHHCCCCCCCCEEECCCCCCEEEE
SDPQVASVGFTEAQAIAAGYATRTSTLPLENVPRALAARDTRGLIKLVADGRTRKLLGAH
CCCCEEECCCHHHHHHHHCCCCCCCCCCHHHCCHHHHHCCCCCCEEEECCCCHHHHHHHE
ILAPEGADSIQTAAMAIRCGLTIDDLAEMIFPYLTTVEGLKLAAQTFDRDVKRLSCCAG
EECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3037534 [H]