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The map label for this gene is 103487869

Identifier: 103487869

GI number: 103487869

Start: 2519256

End: 2521412

Strand: Reverse

Name: 103487869

Synonym: Sala_2388

Alternate gene names: NA

Gene position: 2521412-2519256 (Counterclockwise)

Preceding gene: 103487871

Following gene: 103487868

Centisome position: 75.37

GC content: 67.22

Gene sequence:

>2157_bases
ATGTTGCGACGGATTTTTCCGGGATTCCTGTTGCTTCTGCTTGTTGTCGCCGCGTCGCTCGCGCTGTGGGAACCGCTCAC
TGCCGAGGCGCCGCAGGCGCCCGCCTTCAAGCCCGCCGATGTGCGCATCGCGCGTGACAAGTTCGGCGTGCCGCATATCT
TTGGAAAGACCGACGCCGACGTCGCCTATGGCGTCGCCTATGCGCACGCCGAGGATGATTTTTCGACCTTGCAGGAAGTG
CTGGCGATGACGCGCGGGCGCGCCGGGGCGATGCTCGGGCCGGAGGGCGCCAAGGTCGATTATGCCGAGGCGCTGCTCGG
TGTACGCGCCACCGCGAAGCGCGACTGGCCGCGCCTGCCCGCCGACGTGCGCGCGTTGTTCACCGCCTATGCCGCCGGCC
TCAACCATTATGCCGAGAAACACCCGGACGAGGTGCGGCTGTCGGCATTGTTCCCGGTGACGGGCGAAGACGTCGTTGCG
GGCTTCGTCCTCCGCTCACCCTTTTTCTTCGGGCTCGATTCGGTGCTGGGATCGCTCGTCGAAGGCACGCCGATCGGTCG
CGAGGGCGGCCCCGCGCTTGACGCCACCGGCAAGCTGGTGCCGCGCGCGCTGACGCCGGTCGGCCGTGACCCGGCCGACA
ACGGATCGAACGCGATGGCGGTCGCACCCGCGCGCTCGACCGATGGGGCGACGCGGCTCGTCTCCAATTCGCACCAGCCG
TGGACCGGCGGCGTCGCCTGGTACGAACTGGTCGTCCATTCGGAAGAAGGCTGGGATTTCGCGGGGGCGACCTTCCCCGG
CTCGCCCTATCCCTTCCTTGGTCACAATAAATATCTCGGCTGGACCAATACGGTGAACCGGCCCGACCTGATCGACGTCT
ACAGGCTCGTGCTCGACGCGAGCGGCGAGAAATACCGCTTCGACGGCGGCTGGCGCCCGCTCGAGGAAAAGCGCATCTGG
CTCAAGGTCAAGGTCGGTCCCTTTGTCCTGCCCGTCCCGCGCACCATCTATCGGTCGGTGCATGGACCCGTCGTGAAGAA
CGACAAGGGCGCCTTTGCGATCCGCTATGCGGGGCAGGATCAGGCCAAGATGGTCACCCAATATTACCGGCTGAACAAGG
CCAGGGATTTTGCCCAATGGCGTGCGGCGATGGCGGGGCAGGGTGTGCCCGCGACCAATTTCGTCTATGCCGACGCCAAG
GGCAATATCGGCCTTTTCTATAATGCGATGTTCCCCGACCGGCCCGCAGGGTTCGACTGGCGCGGCGTGCTGCCCGGCGA
CACCTCGGCGGCGCTGTGGACCAGGACATTGCCCTTCGACCGCGTGCCTGCGCTCGTCAATCCCCGCTCGGGCTATGTGA
TGAACGCGAACAACACGCCGTGGGTCGCGGCGGGGCCGGGCGACGAGCTCGATGCCGCCGCATTCTCACCGCTGCTCGGC
ATCGAGGACGACATGACCAACCGCGCGGTGCGGCTGATCGAGCTGTTCGAGGCGTCGGGTCCGATCGACGGAGCGCGGCT
GAAGGCGATCAAATATGACACCGCCTATGCGCGCACCGGCTATGCGAAGGCGTGGATCGACCGGCTGCTTGCGCTCGATA
CCAAGGATGATCGGGCGCTCGCGCAGGCACAGGCGCTGCTCCGAAAATGGGATTGGACGCTCGACGGAAGGGGCAAGGGC
GACGCGCTCGCGCTGATGGTGTTGCGCCCCGCGAACGGCCGGCATTACCAGCGCCGCGCGCCGCCCGATCCGCAGGAGGT
CTTGCGCGACGTCGTTGCGCATCTGCAGGAGCATTTCGGCGGGCTCGACCCCAGGCTCGGGACGGTGCTCCGTCTGCGCC
ACGGTGAGGGGCCGAACCGCATCGACCTGCCGCTCGACGGCGGCAACGACACGGTGCGCGCATCGACCCTGTGGGACGAG
GAGCCCGACGGGCGGCTGAAGGTCCGCCACGGCGACAGCTTCATCATGTTCGTGACGTGGGACCGGGGCGGGCGCGTCCG
CTCCGAATCGATCCAGCCCTTTGGCGCGGCGACCACGCGCCCCGACAGCCCGCATTATAACGATCAGGCGCCGCTGTTCG
TGCGCCATCAGCTCAAGCCCGTGCTGTTCGACCCGGCGGCGCTGAGGGCCAGCGGCGCGCGCTTCTATCGGCCTTGA

Upstream 100 bases:

>100_bases
CAGCCCGTCCGCAAATCGGGCTCGACGAACGACATCGGCTTGCCATTGGACGACATGGCCGCCACAGATGCCGCGGGAGC
CGAAGCGAAGAGGACCAGTG

Downstream 100 bases:

>100_bases
AAGCCGCGCCCCACTGTCCTAAACCATTGATACAGTGCCGTCCGGCACCCGGTTTCCGGCAGGCCATCGCCTGCGCCTTG
ACCCAGGGGATTTTCATGGC

Product: penicillin amidase

Products: NA

Alternate protein names: Glutaryl-7-ACA acylase; 7-beta-(4-carboxybutanamido)cephalosporanic acid acylase; CAD; GL-7-ACA acylase; GCA; Glutaryl-7-ACA acylase subunit alpha; Glutaryl-7-ACA acylase subunit beta [H]

Number of amino acids: Translated: 718; Mature: 718

Protein sequence:

>718_residues
MLRRIFPGFLLLLLVVAASLALWEPLTAEAPQAPAFKPADVRIARDKFGVPHIFGKTDADVAYGVAYAHAEDDFSTLQEV
LAMTRGRAGAMLGPEGAKVDYAEALLGVRATAKRDWPRLPADVRALFTAYAAGLNHYAEKHPDEVRLSALFPVTGEDVVA
GFVLRSPFFFGLDSVLGSLVEGTPIGREGGPALDATGKLVPRALTPVGRDPADNGSNAMAVAPARSTDGATRLVSNSHQP
WTGGVAWYELVVHSEEGWDFAGATFPGSPYPFLGHNKYLGWTNTVNRPDLIDVYRLVLDASGEKYRFDGGWRPLEEKRIW
LKVKVGPFVLPVPRTIYRSVHGPVVKNDKGAFAIRYAGQDQAKMVTQYYRLNKARDFAQWRAAMAGQGVPATNFVYADAK
GNIGLFYNAMFPDRPAGFDWRGVLPGDTSAALWTRTLPFDRVPALVNPRSGYVMNANNTPWVAAGPGDELDAAAFSPLLG
IEDDMTNRAVRLIELFEASGPIDGARLKAIKYDTAYARTGYAKAWIDRLLALDTKDDRALAQAQALLRKWDWTLDGRGKG
DALALMVLRPANGRHYQRRAPPDPQEVLRDVVAHLQEHFGGLDPRLGTVLRLRHGEGPNRIDLPLDGGNDTVRASTLWDE
EPDGRLKVRHGDSFIMFVTWDRGGRVRSESIQPFGAATTRPDSPHYNDQAPLFVRHQLKPVLFDPAALRASGARFYRP

Sequences:

>Translated_718_residues
MLRRIFPGFLLLLLVVAASLALWEPLTAEAPQAPAFKPADVRIARDKFGVPHIFGKTDADVAYGVAYAHAEDDFSTLQEV
LAMTRGRAGAMLGPEGAKVDYAEALLGVRATAKRDWPRLPADVRALFTAYAAGLNHYAEKHPDEVRLSALFPVTGEDVVA
GFVLRSPFFFGLDSVLGSLVEGTPIGREGGPALDATGKLVPRALTPVGRDPADNGSNAMAVAPARSTDGATRLVSNSHQP
WTGGVAWYELVVHSEEGWDFAGATFPGSPYPFLGHNKYLGWTNTVNRPDLIDVYRLVLDASGEKYRFDGGWRPLEEKRIW
LKVKVGPFVLPVPRTIYRSVHGPVVKNDKGAFAIRYAGQDQAKMVTQYYRLNKARDFAQWRAAMAGQGVPATNFVYADAK
GNIGLFYNAMFPDRPAGFDWRGVLPGDTSAALWTRTLPFDRVPALVNPRSGYVMNANNTPWVAAGPGDELDAAAFSPLLG
IEDDMTNRAVRLIELFEASGPIDGARLKAIKYDTAYARTGYAKAWIDRLLALDTKDDRALAQAQALLRKWDWTLDGRGKG
DALALMVLRPANGRHYQRRAPPDPQEVLRDVVAHLQEHFGGLDPRLGTVLRLRHGEGPNRIDLPLDGGNDTVRASTLWDE
EPDGRLKVRHGDSFIMFVTWDRGGRVRSESIQPFGAATTRPDSPHYNDQAPLFVRHQLKPVLFDPAALRASGARFYRP
>Mature_718_residues
MLRRIFPGFLLLLLVVAASLALWEPLTAEAPQAPAFKPADVRIARDKFGVPHIFGKTDADVAYGVAYAHAEDDFSTLQEV
LAMTRGRAGAMLGPEGAKVDYAEALLGVRATAKRDWPRLPADVRALFTAYAAGLNHYAEKHPDEVRLSALFPVTGEDVVA
GFVLRSPFFFGLDSVLGSLVEGTPIGREGGPALDATGKLVPRALTPVGRDPADNGSNAMAVAPARSTDGATRLVSNSHQP
WTGGVAWYELVVHSEEGWDFAGATFPGSPYPFLGHNKYLGWTNTVNRPDLIDVYRLVLDASGEKYRFDGGWRPLEEKRIW
LKVKVGPFVLPVPRTIYRSVHGPVVKNDKGAFAIRYAGQDQAKMVTQYYRLNKARDFAQWRAAMAGQGVPATNFVYADAK
GNIGLFYNAMFPDRPAGFDWRGVLPGDTSAALWTRTLPFDRVPALVNPRSGYVMNANNTPWVAAGPGDELDAAAFSPLLG
IEDDMTNRAVRLIELFEASGPIDGARLKAIKYDTAYARTGYAKAWIDRLLALDTKDDRALAQAQALLRKWDWTLDGRGKG
DALALMVLRPANGRHYQRRAPPDPQEVLRDVVAHLQEHFGGLDPRLGTVLRLRHGEGPNRIDLPLDGGNDTVRASTLWDE
EPDGRLKVRHGDSFIMFVTWDRGGRVRSESIQPFGAATTRPDSPHYNDQAPLFVRHQLKPVLFDPAALRASGARFYRP

Specific function: Catalyzes the deacylation of 7 beta-(4- carboxybutanamido)cephalosporanic acid (glutaryl-7- aminocephalosporanic acid or GL-7-ACA) to 7-aminocephalosporanic acid (7-ACA). Can not efficiently use cephalosporin C (CPC), penicillin G, or ampicillin as substr

COG id: COG2366

COG function: function code R; Protein related to penicillin acylase

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S45 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014395
- InterPro:   IPR002692 [H]

Pfam domain/function: PF01804 Penicil_amidase [H]

EC number: =3.5.1.93 [H]

Molecular weight: Translated: 78846; Mature: 78846

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRRIFPGFLLLLLVVAASLALWEPLTAEAPQAPAFKPADVRIARDKFGVPHIFGKTDAD
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEHHHCCCCEECCCCCCC
VAYGVAYAHAEDDFSTLQEVLAMTRGRAGAMLGPEGAKVDYAEALLGVRATAKRDWPRLP
HHEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
ADVRALFTAYAAGLNHYAEKHPDEVRLSALFPVTGEDVVAGFVLRSPFFFGLDSVLGSLV
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHH
EGTPIGREGGPALDATGKLVPRALTPVGRDPADNGSNAMAVAPARSTDGATRLVSNSHQP
CCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCEEEEECCCCCCCHHHHHCCCCCC
WTGGVAWYELVVHSEEGWDFAGATFPGSPYPFLGHNKYLGWTNTVNRPDLIDVYRLVLDA
CCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
SGEKYRFDGGWRPLEEKRIWLKVKVGPFVLPVPRTIYRSVHGPVVKNDKGAFAIRYAGQD
CCCEEEECCCCCCCCCCEEEEEEEECCEEECCCHHHHHHHCCCEEECCCCCEEEEECCCH
QAKMVTQYYRLNKARDFAQWRAAMAGQGVPATNFVYADAKGNIGLFYNAMFPDRPAGFDW
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCC
RGVLPGDTSAALWTRTLPFDRVPALVNPRSGYVMNANNTPWVAAGPGDELDAAAFSPLLG
CCCCCCCCCHHEEEECCCCCCCCCEECCCCCEEECCCCCCEEEECCCCCCCHHHHCCCCC
IEDDMTNRAVRLIELFEASGPIDGARLKAIKYDTAYARTGYAKAWIDRLLALDTKDDRAL
CCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHCCHHHHHHHHHHHCCCCCHHHH
AQAQALLRKWDWTLDGRGKGDALALMVLRPANGRHYQRRAPPDPQEVLRDVVAHLQEHFG
HHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
GLDPRLGTVLRLRHGEGPNRIDLPLDGGNDTVRASTLWDEEPDGRLKVRHGDSFIMFVTW
CCCCCCCCEEEEECCCCCCEEEEEECCCCCEEEEEECCCCCCCCEEEEEECCEEEEEEEE
DRGGRVRSESIQPFGAATTRPDSPHYNDQAPLFVRHQLKPVLFDPAALRASGARFYRP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEECCHHHHCCCCCCCCC
>Mature Secondary Structure
MLRRIFPGFLLLLLVVAASLALWEPLTAEAPQAPAFKPADVRIARDKFGVPHIFGKTDAD
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEHHHCCCCEECCCCCCC
VAYGVAYAHAEDDFSTLQEVLAMTRGRAGAMLGPEGAKVDYAEALLGVRATAKRDWPRLP
HHEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
ADVRALFTAYAAGLNHYAEKHPDEVRLSALFPVTGEDVVAGFVLRSPFFFGLDSVLGSLV
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHH
EGTPIGREGGPALDATGKLVPRALTPVGRDPADNGSNAMAVAPARSTDGATRLVSNSHQP
CCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCEEEEECCCCCCCHHHHHCCCCCC
WTGGVAWYELVVHSEEGWDFAGATFPGSPYPFLGHNKYLGWTNTVNRPDLIDVYRLVLDA
CCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
SGEKYRFDGGWRPLEEKRIWLKVKVGPFVLPVPRTIYRSVHGPVVKNDKGAFAIRYAGQD
CCCEEEECCCCCCCCCCEEEEEEEECCEEECCCHHHHHHHCCCEEECCCCCEEEEECCCH
QAKMVTQYYRLNKARDFAQWRAAMAGQGVPATNFVYADAKGNIGLFYNAMFPDRPAGFDW
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCC
RGVLPGDTSAALWTRTLPFDRVPALVNPRSGYVMNANNTPWVAAGPGDELDAAAFSPLLG
CCCCCCCCCHHEEEECCCCCCCCCEECCCCCEEECCCCCCEEEECCCCCCCHHHHCCCCC
IEDDMTNRAVRLIELFEASGPIDGARLKAIKYDTAYARTGYAKAWIDRLLALDTKDDRAL
CCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHCCHHHHHHHHHHHCCCCCHHHH
AQAQALLRKWDWTLDGRGKGDALALMVLRPANGRHYQRRAPPDPQEVLRDVVAHLQEHFG
HHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
GLDPRLGTVLRLRHGEGPNRIDLPLDGGNDTVRASTLWDEEPDGRLKVRHGDSFIMFVTW
CCCCCCCCEEEEECCCCCCEEEEEECCCCCEEEEEECCCCCCCCEEEEEECCEEEEEEEE
DRGGRVRSESIQPFGAATTRPDSPHYNDQAPLFVRHQLKPVLFDPAALRASGARFYRP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEECCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11080627 [H]