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The map label for this gene is amtB [H]

Identifier: 103487808

GI number: 103487808

Start: 2453609

End: 2455078

Strand: Direct

Name: amtB [H]

Synonym: Sala_2327

Alternate gene names: 103487808

Gene position: 2453609-2455078 (Clockwise)

Preceding gene: 103487807

Following gene: 103487809

Centisome position: 73.35

GC content: 65.24

Gene sequence:

>1470_bases
ATGACGTTCGCAAACAAGATTGCGGCGGGCGCCGGGGCCGTGGGCCTTTCGCTGTTCGCCGCGCTGCCCGCCTGGGCGCA
AGAGGCCGCAACCGCGGTGGCCGCACCAGCAGCGCCCGCGGCCGAAGCCGCGGCGCCCGCCGCTTTTGTGCCGACCGCCG
AAATGGTCAACAAGGGCGACGTGGCCTGGATGCTCGTCGCCTCGGCGCTGGTGCTGATGATGTCGGTGCCTGCGCTGGCG
CTGTTCTACGGCGGCCTGGTCCGCGCTAAGAATATGCTGTCGGTGCTGATGCAGGTGCTGACCATCGTCTGCGTCGCCGC
GCTGGTCTGGTTCAGCTGGGGCTATTCGATGGCCTTCACCTCCACCGGTACGCCCTGGCCGACGATTGTCGGCGGTCTCG
ACAAGGCGTTCCTTGCCGGCGTCGATGTGACGACCTTTGCCGCCACCTTCTCGAACGGGGTGTATCTCCCCGAATATGTC
TTTGTGATCTTCCAGATGACCTTTGCCTGCATCACCCCGGCACTGATCGTCGGGGCGTTTGCGGAGCGCGTGAAGTTCAC
CCCGCTGATCATCTTCACCGTGCTCTGGCTGACGTTCGCCTATTTCCCGATCGCGCACATGGTGTGGTACTGGGCCGGTC
CGGACTTCCTGCACGCCGCGCCGACCGACATGGGTCTGCTGTGGGGCTGGGGTGCGCTCGACTTTGCCGGCGGCACCGTC
GTCCACATCAACGCGGGTATTGCCGGTCTGGTCGGCTGCCTCGTGATCGGTCCGCGCATGGGTTACAACACCGAACCGAT
GCCCCCGCACAATCTGGTCATGACGATGATCGGCGCCTCGTTGCTGTGGATCGGCTGGTTCGGTTTCAACGCCGGTTCGG
GTCTCGAAGCCAACGTCTTTGGCGCGCTGGCCTTCATCAATACGCTCGTCGCAACTGCGGCTGCGGGCGCGACCTGGGCG
ATCATCGAACAGATCGTACACAAGAAACCGTCGTTGCTGGGCGCTGCGTCCGGCGTGGTCGCCGGTCTCGTCGCGATCAC
CCCGGCGGCGGGCTTCGCCCATCCCGGCACCGCGATCCTGCTCGGCGCCGTCGCCTCGCTGGGCTGCTTCTTCTTCGTCA
CCACGGTGAAAAAGAAGTTCAAATATGACGACTCGCTCGACGTGTTCGGCATTCATGCGGTTGGCGGCATCATCGGCGCG
ATCGGCACCGGCTTCGTCGCCAATCCGGCGTGGGGCGGTCAGGGCTGGATCGATTACACCGCGCCCGTCGCCGTGGCCGG
CGAGTTCGACCTGACTGGCCAGGTGATGACCCAGATCTGGGCCGTCGGCACCACCGTGCTGTGGACCGGTGTGGTCAGCG
CGGTGCTGTTCCTCGGCCTCAAATACACCATCGGCCTGCGCCCGACGAAGGACGTCGAGCAGGAGGGACTCGACCTCGCC
GAACATGGGGAGCGCGCCTACAATCTTTGA

Upstream 100 bases:

>100_bases
CGGCGATGGCAAGATTTTCGTGCTCGACGTGGGTCAGGCGGTGCGCATCCGCACCGGCGAGACCGGCGAGGCGGCTCTCT
AAATGACGAAGGGGGAAACT

Downstream 100 bases:

>100_bases
CGAGACAGGATACCCGTCGCCGCACGTCTCTCTCCTTTCAAAAGACGGCGGCGGGATCCTCACCAGGTTCCTCCTGCGAA
CCACTGGCCGGGGTTTATCC

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MTFANKIAAGAGAVGLSLFAALPAWAQEAATAVAAPAAPAAEAAAPAAFVPTAEMVNKGDVAWMLVASALVLMMSVPALA
LFYGGLVRAKNMLSVLMQVLTIVCVAALVWFSWGYSMAFTSTGTPWPTIVGGLDKAFLAGVDVTTFAATFSNGVYLPEYV
FVIFQMTFACITPALIVGAFAERVKFTPLIIFTVLWLTFAYFPIAHMVWYWAGPDFLHAAPTDMGLLWGWGALDFAGGTV
VHINAGIAGLVGCLVIGPRMGYNTEPMPPHNLVMTMIGASLLWIGWFGFNAGSGLEANVFGALAFINTLVATAAAGATWA
IIEQIVHKKPSLLGAASGVVAGLVAITPAAGFAHPGTAILLGAVASLGCFFFVTTVKKKFKYDDSLDVFGIHAVGGIIGA
IGTGFVANPAWGGQGWIDYTAPVAVAGEFDLTGQVMTQIWAVGTTVLWTGVVSAVLFLGLKYTIGLRPTKDVEQEGLDLA
EHGERAYNL

Sequences:

>Translated_489_residues
MTFANKIAAGAGAVGLSLFAALPAWAQEAATAVAAPAAPAAEAAAPAAFVPTAEMVNKGDVAWMLVASALVLMMSVPALA
LFYGGLVRAKNMLSVLMQVLTIVCVAALVWFSWGYSMAFTSTGTPWPTIVGGLDKAFLAGVDVTTFAATFSNGVYLPEYV
FVIFQMTFACITPALIVGAFAERVKFTPLIIFTVLWLTFAYFPIAHMVWYWAGPDFLHAAPTDMGLLWGWGALDFAGGTV
VHINAGIAGLVGCLVIGPRMGYNTEPMPPHNLVMTMIGASLLWIGWFGFNAGSGLEANVFGALAFINTLVATAAAGATWA
IIEQIVHKKPSLLGAASGVVAGLVAITPAAGFAHPGTAILLGAVASLGCFFFVTTVKKKFKYDDSLDVFGIHAVGGIIGA
IGTGFVANPAWGGQGWIDYTAPVAVAGEFDLTGQVMTQIWAVGTTVLWTGVVSAVLFLGLKYTIGLRPTKDVEQEGLDLA
EHGERAYNL
>Mature_488_residues
TFANKIAAGAGAVGLSLFAALPAWAQEAATAVAAPAAPAAEAAAPAAFVPTAEMVNKGDVAWMLVASALVLMMSVPALAL
FYGGLVRAKNMLSVLMQVLTIVCVAALVWFSWGYSMAFTSTGTPWPTIVGGLDKAFLAGVDVTTFAATFSNGVYLPEYVF
VIFQMTFACITPALIVGAFAERVKFTPLIIFTVLWLTFAYFPIAHMVWYWAGPDFLHAAPTDMGLLWGWGALDFAGGTVV
HINAGIAGLVGCLVIGPRMGYNTEPMPPHNLVMTMIGASLLWIGWFGFNAGSGLEANVFGALAFINTLVATAAAGATWAI
IEQIVHKKPSLLGAASGVVAGLVAITPAAGFAHPGTAILLGAVASLGCFFFVTTVKKKFKYDDSLDVFGIHAVGGIIGAI
GTGFVANPAWGGQGWIDYTAPVAVAGEFDLTGQVMTQIWAVGTTVLWTGVVSAVLFLGLKYTIGLRPTKDVEQEGLDLAE
HGERAYNL

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=449, Percent_Identity=44.097995545657, Blast_Score=350, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI17550182, Length=455, Percent_Identity=27.032967032967, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17550180, Length=448, Percent_Identity=26.3392857142857, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI32564042, Length=449, Percent_Identity=25.1670378619154, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI133931100, Length=451, Percent_Identity=26.6075388026608, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6325396, Length=448, Percent_Identity=33.0357142857143, Blast_Score=216, Evalue=9e-57,
Organism=Saccharomyces cerevisiae, GI6321559, Length=447, Percent_Identity=31.9910514541387, Blast_Score=210, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6324187, Length=437, Percent_Identity=35.2402745995423, Blast_Score=210, Evalue=4e-55,
Organism=Drosophila melanogaster, GI161078314, Length=439, Percent_Identity=26.4236902050114, Blast_Score=101, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 51000; Mature: 50869

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCHHHCCC
>Mature Secondary Structure 
TFANKIAAGAGAVGLSLFAALPAWAQEAATAVAAPAAPAAEAAAPAAFVPTAEMVNKGD
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCHHHHCCCC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HCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]