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The map label for this gene is lpd3 [H]

Identifier: 103487706

GI number: 103487706

Start: 2336317

End: 2337714

Strand: Reverse

Name: lpd3 [H]

Synonym: Sala_2225

Alternate gene names: 103487706

Gene position: 2337714-2336317 (Counterclockwise)

Preceding gene: 103487707

Following gene: 103487704

Centisome position: 69.88

GC content: 65.02

Gene sequence:

>1398_bases
ATGGCTGATTACGACTACGACGTCCTTGTCATTGGTGCCGGTCCCGGCGGTTATGTCGCGGCGATCCGCGCGGCGCAGCT
GGGGCTCAAGACCGCGTGCGCCGAGGGGCGCGCGACGCTGGGCGGCACCTGCCTCAACGTCGGCTGTATCCCGTCGAAGG
CGATGCTGCACGCGTCGGAGTATTTTGACGCCGCGGCGAACGGGTCGATGGCGGCGATGGGCATCAGGGTGAAGCCCGAA
CTTGACCTGGACACCATGCACGGCCAGCGCCGCGATGCGGTCAAGGGGCTGACCGGCGGCATCGAGTTCCTGTTCAGGAA
GAACAAGGTCGACTGGCTGAAGGGCTATGCCCGGTTCACGTCGAAGGACAGTGTCGAGGTCGCGGGCAAGAGTTATCGCG
CGAAGAATATCATCATCGCGACGGGATCGTCGGTGACGCCGCTGCCGGGCGTCGAGGTCGATAACGACAAGGGCGTGATC
GTCGATTCGACCGGTGCGCTCGAACTCGCCAAGGTGCCGGGGCATATGGTCGTGATCGGCGGCGGCGTGATCGGGCTGGA
GCTGGGCAGCGTGTGGCGCCGCCTGGGCGCCAAGGTCACCTGCGTCGAGTTTCTCGACCAGATCCTGCCCGGCATGGACG
GCGATGTGCGCAAGGAAGCGAACAAGATCTTCAAGAAGCAGGGGATCGAGTTCAAACTGAAAACCAAGGTGACGAAGGCC
GAGGTCAAGGGCAAGAAGGCCGTCCTGACGCTCGAACCCGCGGCGGGTGGCGAGTCCGAAACGCTGGAGGCCGACGTCGT
GCTCGTGTCGATCGGACGGCGGCCGAACACCGACGGCCTTGCGCTCGACAAGGCGGGGCTGGAGGTCAACCAGCGCGGCC
AGATCGAGATCGACCATGATTTTCGCACCGCGATCGACGGCATCTGGGCGATCGGCGACGTCGTGCCGGGGCCGATGCTC
GCGCACAAGGCCGAGGATGAAGGCATCGCCTGCGCCGAGAATATCGCAGGGCAGACGGGGATCGTGAACCATGATGTCAT
CCCGTCGGTCGTCTATACCTGGCCTGAAATCGCCGGCGTCGGGCTGACCGAGGAACAGGCGAAGGAAAAGGGTGAGGTCA
GGGTCGGCAAGTTCCCGATGCTCGCGAACAGCCGCGCCAAGACCAACCACGAACCCGACGGTTTCGTGAAGGTGATCGCC
GATGCCAGGACCGACCGCGTGCTGGGCGTGTGGTGCATCGCCAGCGTCGCGGGCACGATGATCGCGCAGGCGGCGCAGGC
GATGGAGTTCGGCGCGACGTCCGAAGACATCGCCTATACCTGCCACGCGCACCCGACGCACAGCGAGGCGATCAAGGAAG
CCGCGATGGCGGTGACAGGCAAGCCGATCCACATCTGA

Upstream 100 bases:

>100_bases
TGATCGAACGCGATAATCCGCAATGGCTGGATTTGTTCGACAGGCTATCCCTTTGACGCCCTGGGTTCCTGCGTTCGCAG
GAACGCATATGGAGTAAATC

Downstream 100 bases:

>100_bases
CCGGCGCACATCTGGTCGGTGCGGGAGAGCGGCGGCGCGTTACGATGCCATCATCGCGTGCCACCGGTCCATCGCCGCGG
CGCTCCAGACATCTTCCCGC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase 3; LPD-3 [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MADYDYDVLVIGAGPGGYVAAIRAAQLGLKTACAEGRATLGGTCLNVGCIPSKAMLHASEYFDAAANGSMAAMGIRVKPE
LDLDTMHGQRRDAVKGLTGGIEFLFRKNKVDWLKGYARFTSKDSVEVAGKSYRAKNIIIATGSSVTPLPGVEVDNDKGVI
VDSTGALELAKVPGHMVVIGGGVIGLELGSVWRRLGAKVTCVEFLDQILPGMDGDVRKEANKIFKKQGIEFKLKTKVTKA
EVKGKKAVLTLEPAAGGESETLEADVVLVSIGRRPNTDGLALDKAGLEVNQRGQIEIDHDFRTAIDGIWAIGDVVPGPML
AHKAEDEGIACAENIAGQTGIVNHDVIPSVVYTWPEIAGVGLTEEQAKEKGEVRVGKFPMLANSRAKTNHEPDGFVKVIA
DARTDRVLGVWCIASVAGTMIAQAAQAMEFGATSEDIAYTCHAHPTHSEAIKEAAMAVTGKPIHI

Sequences:

>Translated_465_residues
MADYDYDVLVIGAGPGGYVAAIRAAQLGLKTACAEGRATLGGTCLNVGCIPSKAMLHASEYFDAAANGSMAAMGIRVKPE
LDLDTMHGQRRDAVKGLTGGIEFLFRKNKVDWLKGYARFTSKDSVEVAGKSYRAKNIIIATGSSVTPLPGVEVDNDKGVI
VDSTGALELAKVPGHMVVIGGGVIGLELGSVWRRLGAKVTCVEFLDQILPGMDGDVRKEANKIFKKQGIEFKLKTKVTKA
EVKGKKAVLTLEPAAGGESETLEADVVLVSIGRRPNTDGLALDKAGLEVNQRGQIEIDHDFRTAIDGIWAIGDVVPGPML
AHKAEDEGIACAENIAGQTGIVNHDVIPSVVYTWPEIAGVGLTEEQAKEKGEVRVGKFPMLANSRAKTNHEPDGFVKVIA
DARTDRVLGVWCIASVAGTMIAQAAQAMEFGATSEDIAYTCHAHPTHSEAIKEAAMAVTGKPIHI
>Mature_464_residues
ADYDYDVLVIGAGPGGYVAAIRAAQLGLKTACAEGRATLGGTCLNVGCIPSKAMLHASEYFDAAANGSMAAMGIRVKPEL
DLDTMHGQRRDAVKGLTGGIEFLFRKNKVDWLKGYARFTSKDSVEVAGKSYRAKNIIIATGSSVTPLPGVEVDNDKGVIV
DSTGALELAKVPGHMVVIGGGVIGLELGSVWRRLGAKVTCVEFLDQILPGMDGDVRKEANKIFKKQGIEFKLKTKVTKAE
VKGKKAVLTLEPAAGGESETLEADVVLVSIGRRPNTDGLALDKAGLEVNQRGQIEIDHDFRTAIDGIWAIGDVVPGPMLA
HKAEDEGIACAENIAGQTGIVNHDVIPSVVYTWPEIAGVGLTEEQAKEKGEVRVGKFPMLANSRAKTNHEPDGFVKVIAD
ARTDRVLGVWCIASVAGTMIAQAAQAMEFGATSEDIAYTCHAHPTHSEAIKEAAMAVTGKPIHI

Specific function: LPD-3 may substitute for lipoamide dehydrogenase of the 2-oxoglutarate dehydrogenase and pyruvate multienzyme complexes when the latter is inactive or missing [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=471, Percent_Identity=53.927813163482, Blast_Score=471, Evalue=1e-133,
Organism=Homo sapiens, GI50301238, Length=476, Percent_Identity=29.6218487394958, Blast_Score=175, Evalue=7e-44,
Organism=Homo sapiens, GI148277065, Length=491, Percent_Identity=27.6985743380855, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI33519430, Length=491, Percent_Identity=27.6985743380855, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI33519428, Length=491, Percent_Identity=27.6985743380855, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI33519426, Length=491, Percent_Identity=27.6985743380855, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI148277071, Length=491, Percent_Identity=27.6985743380855, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI291045266, Length=475, Percent_Identity=26.9473684210526, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI22035672, Length=470, Percent_Identity=28.5106382978723, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI291045268, Length=470, Percent_Identity=25.1063829787234, Blast_Score=101, Evalue=2e-21,
Organism=Escherichia coli, GI1786307, Length=459, Percent_Identity=42.0479302832244, Blast_Score=320, Evalue=8e-89,
Organism=Escherichia coli, GI87082354, Length=465, Percent_Identity=30.3225806451613, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI1789915, Length=435, Percent_Identity=32.183908045977, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI87081717, Length=467, Percent_Identity=29.9785867237687, Blast_Score=180, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI32565766, Length=468, Percent_Identity=52.5641025641026, Blast_Score=468, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17557007, Length=476, Percent_Identity=29.8319327731092, Blast_Score=148, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI71983429, Length=465, Percent_Identity=31.1827956989247, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71983419, Length=465, Percent_Identity=31.1827956989247, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI71982272, Length=488, Percent_Identity=27.0491803278689, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17559934, Length=201, Percent_Identity=30.3482587064677, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6321091, Length=475, Percent_Identity=51.3684210526316, Blast_Score=441, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6325240, Length=484, Percent_Identity=35.7438016528926, Blast_Score=253, Evalue=4e-68,
Organism=Saccharomyces cerevisiae, GI6325166, Length=471, Percent_Identity=29.0870488322718, Blast_Score=158, Evalue=2e-39,
Organism=Drosophila melanogaster, GI21358499, Length=473, Percent_Identity=53.4883720930233, Blast_Score=489, Evalue=1e-138,
Organism=Drosophila melanogaster, GI24640553, Length=477, Percent_Identity=29.559748427673, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24640549, Length=477, Percent_Identity=29.559748427673, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24640551, Length=477, Percent_Identity=29.559748427673, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI17737741, Length=486, Percent_Identity=26.3374485596708, Blast_Score=119, Evalue=4e-27,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49149; Mature: 49018

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADYDYDVLVIGAGPGGYVAAIRAAQLGLKTACAEGRATLGGTCLNVGCIPSKAMLHASE
CCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHH
YFDAAANGSMAAMGIRVKPELDLDTMHGQRRDAVKGLTGGIEFLFRKNKVDWLKGYARFT
HHHHCCCCCEEEEEEEECCCCCCHHHCCCHHHHHHHHHHHHHEEEECCCHHHHHHHHHHC
SKDSVEVAGKSYRAKNIIIATGSSVTPLPGVEVDNDKGVIVDSTGALELAKVPGHMVVIG
CCCCEEECCCCCCCCEEEEECCCCCCCCCCEEECCCCCEEEECCCCEEEEECCCEEEEEE
GGVIGLELGSVWRRLGAKVTCVEFLDQILPGMDGDVRKEANKIFKKQGIEFKLKTKVTKA
CCEEEEHHHHHHHHHCCEEEHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEECEEHH
EVKGKKAVLTLEPAAGGESETLEADVVLVSIGRRPNTDGLALDKAGLEVNQRGQIEIDHD
HCCCCEEEEEEECCCCCCCCCEEEEEEEEEECCCCCCCCEEEECCCCCCCCCCCEEECCC
FRTAIDGIWAIGDVVPGPMLAHKAEDEGIACAENIAGQTGIVNHDVIPSVVYTWPEIAGV
HHHHHHHHHHHHHCCCCCHHEECCCCCCCCHHHHCCCCCCCCCCCCCCHHHHCCHHHCCC
GLTEEQAKEKGEVRVGKFPMLANSRAKTNHEPDGFVKVIADARTDRVLGVWCIASVAGTM
CCCHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
IAQAAQAMEFGATSEDIAYTCHAHPTHSEAIKEAAMAVTGKPIHI
HHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ADYDYDVLVIGAGPGGYVAAIRAAQLGLKTACAEGRATLGGTCLNVGCIPSKAMLHASE
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHH
YFDAAANGSMAAMGIRVKPELDLDTMHGQRRDAVKGLTGGIEFLFRKNKVDWLKGYARFT
HHHHCCCCCEEEEEEEECCCCCCHHHCCCHHHHHHHHHHHHHEEEECCCHHHHHHHHHHC
SKDSVEVAGKSYRAKNIIIATGSSVTPLPGVEVDNDKGVIVDSTGALELAKVPGHMVVIG
CCCCEEECCCCCCCCEEEEECCCCCCCCCCEEECCCCCEEEECCCCEEEEECCCEEEEEE
GGVIGLELGSVWRRLGAKVTCVEFLDQILPGMDGDVRKEANKIFKKQGIEFKLKTKVTKA
CCEEEEHHHHHHHHHCCEEEHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEECEEHH
EVKGKKAVLTLEPAAGGESETLEADVVLVSIGRRPNTDGLALDKAGLEVNQRGQIEIDHD
HCCCCEEEEEEECCCCCCCCCEEEEEEEEEECCCCCCCCEEEECCCCCCCCCCCEEECCC
FRTAIDGIWAIGDVVPGPMLAHKAEDEGIACAENIAGQTGIVNHDVIPSVVYTWPEIAGV
HHHHHHHHHHHHHCCCCCHHEECCCCCCCCHHHHCCCCCCCCCCCCCCHHHHCCHHHCCC
GLTEEQAKEKGEVRVGKFPMLANSRAKTNHEPDGFVKVIADARTDRVLGVWCIASVAGTM
CCCHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
IAQAAQAMEFGATSEDIAYTCHAHPTHSEAIKEAAMAVTGKPIHI
HHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]