Click here to switch to the map view.

The map label for this gene is proB

Identifier: 103487700

GI number: 103487700

Start: 2329466

End: 2330572

Strand: Direct

Name: proB

Synonym: Sala_2219

Alternate gene names: 103487700

Gene position: 2329466-2330572 (Clockwise)

Preceding gene: 103487697

Following gene: 103487701

Centisome position: 69.64

GC content: 72.27

Gene sequence:

>1107_bases
ATGAGCCTGTTTCCGCCCGCCTCCTGCCCGCGACTGATCGTCAAGATCGGGTCGGCGCTGCTCGTCGATCCGGGCGGCGC
GGTGCGGCGCGACTGGCTCACAGGCATCGCCGCCGACATCGCGGAACGCGCCCGCGCCGGGCAACAGGTCGCGGTCGTCT
CGTCGGGTGCGATCGCGCTCGGCGCGCGGCGGCTGGGGCTTGCCAAGGGCGGCCGCGCGAGCCTCGAAGATGCACAGGCG
GCCGCCGCGACGGGGCAGATCGCGCTCAGCCAGGTGTGGGCCGAGGTGCTCGGCGCCGAGGGGCTGACGGCGGCACAGAT
GCTTGTGACGCTCGGTGACCTCGAACATCGGCGCCGCTACCTCAACGCCGCGGCGACGCTCGATCGCCTGCTCAGCCTGG
GCGTCGTCCCGATCATCAACGAGAATGACAGCGTCGCGACCGAGGAAATCCGCTTCGGCGACAACGACCGGCTCGCCGCG
CGCGTCGCGCAGGCCGCCGCGGCGCGCGGCGTCATCCTTCTCTCTGACATCGACGGACTCTACGACCGCAACCCGGCGCA
ACCGGGCGCCGTACATATCGAGCGAATCGAGCGGATTGACGCAGCGATCGAAGCGATGGCCGACACGGGGTCCGCATCGG
GCATGGGATCGGGCGGCATGGTGTCGAAAATCGCCGCCGCGCGCATCGCCAACGCCGCGGGCGCGCATCTGGCCATCGCT
TCGGGTCGGATCGACCGTCCGCTGTCGACCGCGGCGCGGCACAGCCTGTTCGTCGCCGAGCGCGGCGCGAGCGCGCGCAA
GGCGTGGCTCGCGGGCGGGCTGACCGCCGAAGGGCGGCTGACGATCGACGCGGGCGCGGTCAAGGCGCTGGTGGGCGGCG
CGAGCCTGCTCGCCGCCGGGGTAACTGCGGTATCAGGCAGCTTCGCGCGCGGCGACATCCTCGATATCGTCGGACCCGAC
GGGCGCGTCGTCGCGCGCGGCCTGTCCGAATATCCTGCGGCCGATGCGGCTGCAATCGTCGGGCTCGGCCGCGACGCGCA
GGAGGCCGCGCTCGGCTATGCGCCGCGCAGCGCCATCGTCCACCGCGACCATATGGTGCTGCTGTGA

Upstream 100 bases:

>100_bases
GGCAAACCTATGGGACCGCGCCGCGCGCGTCCAGCGGCGGCGCGCGGGGCAAACGACATTCGCATTGGCGCGCGGGCGTC
GCCGCGCTATCGGCGGCGGC

Downstream 100 bases:

>100_bases
TCCTCCCCTGCAAGGGGAGGGGGACCACCCGGAGGGTGGTGGAGGGGCACGCGCAGCCAAATGCGGCGTTCGACCTCCTG
CGCCCCTCCCCCATGCTTCG

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MSLFPPASCPRLIVKIGSALLVDPGGAVRRDWLTGIAADIAERARAGQQVAVVSSGAIALGARRLGLAKGGRASLEDAQA
AAATGQIALSQVWAEVLGAEGLTAAQMLVTLGDLEHRRRYLNAAATLDRLLSLGVVPIINENDSVATEEIRFGDNDRLAA
RVAQAAAARGVILLSDIDGLYDRNPAQPGAVHIERIERIDAAIEAMADTGSASGMGSGGMVSKIAAARIANAAGAHLAIA
SGRIDRPLSTAARHSLFVAERGASARKAWLAGGLTAEGRLTIDAGAVKALVGGASLLAAGVTAVSGSFARGDILDIVGPD
GRVVARGLSEYPAADAAAIVGLGRDAQEAALGYAPRSAIVHRDHMVLL

Sequences:

>Translated_368_residues
MSLFPPASCPRLIVKIGSALLVDPGGAVRRDWLTGIAADIAERARAGQQVAVVSSGAIALGARRLGLAKGGRASLEDAQA
AAATGQIALSQVWAEVLGAEGLTAAQMLVTLGDLEHRRRYLNAAATLDRLLSLGVVPIINENDSVATEEIRFGDNDRLAA
RVAQAAAARGVILLSDIDGLYDRNPAQPGAVHIERIERIDAAIEAMADTGSASGMGSGGMVSKIAAARIANAAGAHLAIA
SGRIDRPLSTAARHSLFVAERGASARKAWLAGGLTAEGRLTIDAGAVKALVGGASLLAAGVTAVSGSFARGDILDIVGPD
GRVVARGLSEYPAADAAAIVGLGRDAQEAALGYAPRSAIVHRDHMVLL
>Mature_367_residues
SLFPPASCPRLIVKIGSALLVDPGGAVRRDWLTGIAADIAERARAGQQVAVVSSGAIALGARRLGLAKGGRASLEDAQAA
AATGQIALSQVWAEVLGAEGLTAAQMLVTLGDLEHRRRYLNAAATLDRLLSLGVVPIINENDSVATEEIRFGDNDRLAAR
VAQAAAARGVILLSDIDGLYDRNPAQPGAVHIERIERIDAAIEAMADTGSASGMGSGGMVSKIAAARIANAAGAHLAIAS
GRIDRPLSTAARHSLFVAERGASARKAWLAGGLTAEGRLTIDAGAVKALVGGASLLAAGVTAVSGSFARGDILDIVGPDG
RVVARGLSEYPAADAAAIVGLGRDAQEAALGYAPRSAIVHRDHMVLL

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI62912457, Length=264, Percent_Identity=32.5757575757576, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI21361368, Length=266, Percent_Identity=32.3308270676692, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1786437, Length=362, Percent_Identity=38.121546961326, Blast_Score=200, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17569969, Length=250, Percent_Identity=33.2, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17569967, Length=250, Percent_Identity=33.2, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6320506, Length=417, Percent_Identity=31.4148681055156, Blast_Score=170, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6321822, Length=414, Percent_Identity=28.9855072463768, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI21357643, Length=274, Percent_Identity=32.8467153284672, Blast_Score=111, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_SPHAL (Q1GQZ4)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_617261.1
- ProteinModelPortal:   Q1GQZ4
- SMR:   Q1GQZ4
- GeneID:   4080290
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_2219
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- ProtClustDB:   PRK05429
- BioCyc:   SALA317655:SALA_2219-MONOMER
- BRENDA:   2.7.2.11
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 37406; Mature: 37275

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFPPASCPRLIVKIGSALLVDPGGAVRRDWLTGIAADIAERARAGQQVAVVSSGAIAL
CCCCCCCCCHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEE
GARRLGLAKGGRASLEDAQAAAATGQIALSQVWAEVLGAEGLTAAQMLVTLGDLEHRRRY
CHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LNAAATLDRLLSLGVVPIINENDSVATEEIRFGDNDRLAARVAQAAAARGVILLSDIDGL
HHHHHHHHHHHHCCCEEEECCCCCCCHHHEECCCCHHHHHHHHHHHHHCCEEEEECCCCH
YDRNPAQPGAVHIERIERIDAAIEAMADTGSASGMGSGGMVSKIAAARIANAAGAHLAIA
HCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEE
SGRIDRPLSTAARHSLFVAERGASARKAWLAGGLTAEGRLTIDAGAVKALVGGASLLAAG
CCCCCCCHHHHHHHHEEEECCCCCHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHH
VTAVSGSFARGDILDIVGPDGRVVARGLSEYPAADAAAIVGLGRDAQEAALGYAPRSAIV
HHHHCCCCCCCCEEEEECCCCHHHHCCHHHCCCCCHHHHEECCCCHHHHHHCCCCCHHHE
HRDHMVLL
ECCCEEEC
>Mature Secondary Structure 
SLFPPASCPRLIVKIGSALLVDPGGAVRRDWLTGIAADIAERARAGQQVAVVSSGAIAL
CCCCCCCCHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEE
GARRLGLAKGGRASLEDAQAAAATGQIALSQVWAEVLGAEGLTAAQMLVTLGDLEHRRRY
CHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LNAAATLDRLLSLGVVPIINENDSVATEEIRFGDNDRLAARVAQAAAARGVILLSDIDGL
HHHHHHHHHHHHCCCEEEECCCCCCCHHHEECCCCHHHHHHHHHHHHHCCEEEEECCCCH
YDRNPAQPGAVHIERIERIDAAIEAMADTGSASGMGSGGMVSKIAAARIANAAGAHLAIA
HCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEE
SGRIDRPLSTAARHSLFVAERGASARKAWLAGGLTAEGRLTIDAGAVKALVGGASLLAAG
CCCCCCCHHHHHHHHEEEECCCCCHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHH
VTAVSGSFARGDILDIVGPDGRVVARGLSEYPAADAAAIVGLGRDAQEAALGYAPRSAIV
HHHHCCCCCCCCEEEEECCCCHHHHCCHHHCCCCCHHHHEECCCCHHHHHHCCCCCHHHE
HRDHMVLL
ECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA