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The map label for this gene is mcl2 [H]

Identifier: 103487695

GI number: 103487695

Start: 2325907

End: 2326710

Strand: Reverse

Name: mcl2 [H]

Synonym: Sala_2214

Alternate gene names: 103487695

Gene position: 2326710-2325907 (Counterclockwise)

Preceding gene: 103487698

Following gene: 103487694

Centisome position: 69.55

GC content: 71.02

Gene sequence:

>804_bases
ATGACCGACTTCGCCCCCCGCTCGCTGCTCTATGTCCCCGGTTCGAACGCTCGCGCGCTGGAAAAGGCGCAAGCGCTCGC
CGCCGATATGCTGATTATCGACCTCGAGGATGCGGTGCCGGCCGACCGCAAGGACGACGCGCGCGCGGCGATGCGCGCGG
CGGTGACGGCGGGCTATCCGGGCAAGCGCGTCGCGGTGCGGATCAACGGGGCGGGGACCGCGCATCAGGCGGCGGACATC
GACGCGCTCGCGGGACTGCCGCTCGATGCCGTCGTGCTGCCCAAGGTCGACGCCATCGCCGACCTCGCCCCAGCGCGCGG
GCTCGGCTTGCCGCTGCTCGCGATGATCGAGACGCCCGCCGCCATCTATGCCGCGCGCGACATCGCTGCGGACCCCGGCG
TGGCGGGTCTGATTGCGGGGCTCAACGATCTCGCGCACGAATTGAAACTGCCCGACGGGACCGATCGCGACGCGATGAGC
CATGCGATCCAGGCGATCGTACTGGCCGCGCGCGCGGGCGGGGTGTGGTGCTTCGACGGGGTTTATAATGCGATCGACGA
TGTCGCAGGTTTCGCCGCCGAAGCCGCCGAGGGGCGGCGGCTGGGCTTTGACGGCAAGACGCTGATCCACCCGTCGCAGG
TCGATCCGTGCAACGCCGCCTTTGCTCCGACGGTGCGCGAGATTGCGGCGGCCGAGGCGCTGGTCGCGGCGGCGACCGGC
GGCGCACAGCGGCACGAAGGCCGGATGATCGAGGATATGCACGTCGCCGCGGCGCGGGCGCTGCTGGCGCGGGCAAGGGG
ATAA

Upstream 100 bases:

>100_bases
GAGTGCGAATTCCATCGATCATCCCCTCTTGCAAACAAGCGTCTTTGCAAACCCGCCTTCCTTATTGCGGCAATGGCGCG
GCTGCGCCACAGAAAAGCGC

Downstream 100 bases:

>100_bases
CCTCAATTCGTCACCCCGGCGGAGGCCGGGGTCTCGACCTAGCGTTACAATGCACCGGCGAGATCCCGGCCTTCGCCGGG
ATGACGAATTGAGAAAACGG

Product: HpcH/HpaI aldolase

Products: acetate; oxaloacetate

Alternate protein names: (3S)-malyl-CoA thioesterase [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MTDFAPRSLLYVPGSNARALEKAQALAADMLIIDLEDAVPADRKDDARAAMRAAVTAGYPGKRVAVRINGAGTAHQAADI
DALAGLPLDAVVLPKVDAIADLAPARGLGLPLLAMIETPAAIYAARDIAADPGVAGLIAGLNDLAHELKLPDGTDRDAMS
HAIQAIVLAARAGGVWCFDGVYNAIDDVAGFAAEAAEGRRLGFDGKTLIHPSQVDPCNAAFAPTVREIAAAEALVAAATG
GAQRHEGRMIEDMHVAAARALLARARG

Sequences:

>Translated_267_residues
MTDFAPRSLLYVPGSNARALEKAQALAADMLIIDLEDAVPADRKDDARAAMRAAVTAGYPGKRVAVRINGAGTAHQAADI
DALAGLPLDAVVLPKVDAIADLAPARGLGLPLLAMIETPAAIYAARDIAADPGVAGLIAGLNDLAHELKLPDGTDRDAMS
HAIQAIVLAARAGGVWCFDGVYNAIDDVAGFAAEAAEGRRLGFDGKTLIHPSQVDPCNAAFAPTVREIAAAEALVAAATG
GAQRHEGRMIEDMHVAAARALLARARG
>Mature_266_residues
TDFAPRSLLYVPGSNARALEKAQALAADMLIIDLEDAVPADRKDDARAAMRAAVTAGYPGKRVAVRINGAGTAHQAADID
ALAGLPLDAVVLPKVDAIADLAPARGLGLPLLAMIETPAAIYAARDIAADPGVAGLIAGLNDLAHELKLPDGTDRDAMSH
AIQAIVLAARAGGVWCFDGVYNAIDDVAGFAAEAAEGRRLGFDGKTLIHPSQVDPCNAAFAPTVREIAAAEALVAAATGG
AQRHEGRMIEDMHVAAARALLARARG

Specific function: Catalyzes the hydrolysis of (3S)-malyl-CoA to (3S)- malate and free CoA. Inactive towards beta-methylmalyl-CoA and other CoA esters [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=276, Percent_Identity=27.1739130434783, Blast_Score=90, Evalue=2e-18,
Organism=Escherichia coli, GI87081764, Length=240, Percent_Identity=35.8333333333333, Blast_Score=114, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17557458, Length=281, Percent_Identity=28.1138790035587, Blast_Score=81, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 27387; Mature: 27255

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDFAPRSLLYVPGSNARALEKAQALAADMLIIDLEDAVPADRKDDARAAMRAAVTAGYP
CCCCCCCEEEEECCCCHHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHCCCC
GKRVAVRINGAGTAHQAADIDALAGLPLDAVVLPKVDAIADLAPARGLGLPLLAMIETPA
CCEEEEEECCCCCCCHHCCHHHHCCCCCCEEECCCHHHHHHHCCCCCCCCHHHHHHCCCH
AIYAARDIAADPGVAGLIAGLNDLAHELKLPDGTDRDAMSHAIQAIVLAARAGGVWCFDG
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEHHH
VYNAIDDVAGFAAEAAEGRRLGFDGKTLIHPSQVDPCNAAFAPTVREIAAAEALVAAATG
HHHHHHHHHHHHHHHCCCCEECCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
GAQRHEGRMIEDMHVAAARALLARARG
CCHHHCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDFAPRSLLYVPGSNARALEKAQALAADMLIIDLEDAVPADRKDDARAAMRAAVTAGYP
CCCCCCEEEEECCCCHHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHCCCC
GKRVAVRINGAGTAHQAADIDALAGLPLDAVVLPKVDAIADLAPARGLGLPLLAMIETPA
CCEEEEEECCCCCCCHHCCHHHHCCCCCCEEECCCHHHHHHHCCCCCCCCHHHHHHCCCH
AIYAARDIAADPGVAGLIAGLNDLAHELKLPDGTDRDAMSHAIQAIVLAARAGGVWCFDG
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEHHH
VYNAIDDVAGFAAEAAEGRRLGFDGKTLIHPSQVDPCNAAFAPTVREIAAAEALVAAATG
HHHHHHHHHHHHHHHCCCCEECCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
GAQRHEGRMIEDMHVAAARALLARARG
CCHHHCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} 0.0565 {oxaoacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA